Beruflich Dokumente
Kultur Dokumente
Michael A. Wilson,1 Timothy R. Brubaker,2 Andrea M. Mastro,3 Michael M. Micci,4 Sven G. Biln,5 and Sean D. Knecht6
1Department
of Electrical Engineering; 2Department of Electrical Engineering; 3Department of Biochemistry and Molecular Biology; 4Department of Aerospace
Engineering; 5Department of Electrical Engineering; 6Applied Research Laboratory
Abstract
Results
Experimental Design
The 22-gauge needle is 2 long and serves as the high voltage electrode.
The grounding electrode is placed approximately 1/8 from the tip of the syringe.
The syringe is sheathed with PEEK (polyether ether ketone).
PEEK has a high dielectric strength used as a barrier to prevent arc production.
The diameter of the device with the PEEK is equivalent to a 16-gauge needle.
Helium flows axially through the syringe.
Gas is controlled by the mass flow controller calibrated at 70 F, and 14.7 psia.
The flow rate can be controlled over the range of 16775 mL/min.
An Agilent 33220A Function Generator generates a 5-kHz, 2-Vpp sine wave.
This wave is amplified 1000 to 2 kV by a Trek 10-40 amplifier.
Increasing the voltage or mass flow rate increases the length and visible intensity of
the plasma plume.
(a)
(b)
(c)
(d)
Three cell culture wells were exposed to helium and three wells exposed to plasma.
Two wells were exposed for 60 seconds and four were exposed for 180 seconds.
The mass flow rate was set to 775 mL/min.
Plasma exposed cultures had voids in the cell culture that were 23 the diameter of the
voids in the helium-exposed cultures.
Cell destruction was observed to occur for a larger area in the case of the plasma jet.
This indicates an additional mechanism of cell destruction is active in the lowtemperature plasma beyond the kinetic energy of the jet molecules.
Syringe
within
PEEK
Ground wire
wrapped around
syringe
The hypothesis is that the reactive oxygen species generated in the plasma plume are
responsible for the destructive effects due to increased oxidative stress on the cells.
The generation of reactive oxygen species in the plasma plume and the subsequent
oxidative stress on the cells is the proposed method of action.
Further experimentation is planned to quantify the concentration of reactive chemical
species and evaluate this hypothesis.
We will determine why pure helium exposure results in cell destruction.
Different gases will be used in future experiments as well as longer exposure times.
Larger diameter wells and a greater volume of PBS will also be used.
Acknowledgments
Plasma
Discharge
The authors wish to thank D. Sosnoski, for helping prepare the cell cultures. The BC
line (MDA-MB-231, ATCC-HTB 26 presumptive equivalent) was initially provided by
Dr. D. Welch, University of Alabama at Birmingham.
Contacts:
Michael A. Wilson
Timothy R. Brubaker
Andrea M. Mastro
Michael M. Micci
Sven G. Biln
Sean D. Knecht
maw5595@psu.edu
trb5084@psu.edu
a36@psu.edu
x0c@engr.psu.edu
sbilen@psu.edu
sdk149@psu.edu