Beruflich Dokumente
Kultur Dokumente
http://www.elsevier.com/locate/biombioe
INRA, UR50, Laboratoire de Biotechnologie de LEnvironnement, Avenues des Etangs, 11100 Narbonne, France
Naskeo, Avenues des Etangs, 11100 Narbonne, France
article info
abstract
Article history:
Research into the development of renewable and sustainable fuels has been a major
concern during last decades. Microalgae, as a potential resource, have gained great
attention for energy purposes. In this context, anaerobic digestion seems to be the most
8 February 2012
direct energy generation process. Nevertheless, the efficiency of this process is hampered
due to the hard cell wall of some microalgae. In order to enhance its anaerobic biode-
gradability, the present research investigated the effect of thermal pretreatment at two
temperatures (70 and 90 C) applied to Scenedesmus biomass. No differences were detected
Keywords:
in terms of organic matter or ammonium release upon the two tested temperatures.
Microalgae
Nevertheless, a different fact was observed for their anaerobic biodegradability. While raw
Photobioreactor
Anaerobic digestion
Pretreatment
were obtained for both temperatures along the pretreatment period, the damage caused in
Cell wall
the cell wall at 90 C seemed to be greater and rendered this substrate readily degradable
Biofuel
1.
Introduction
* Corresponding author.
E-mail address: cgonfer@iq.uva.es (C. Gonzalez-Fernandez).
0961-9534/$ e see front matter 2012 Elsevier Ltd. All rights reserved.
doi:10.1016/j.biombioe.2012.02.008
106
b i o m a s s a n d b i o e n e r g y 4 0 ( 2 0 1 2 ) 1 0 5 e1 1 1
2.
2.1.
Scenedesmus biomass and photobioreactor culture
conditions
2.2.
2.3.
The microalga Scenedesmus sp. was collected during spring
from an open pond of the urban wastewater treatment plant
located at the geo-coordinates 43 250 4000 North, 3 360 2100 East
(Meze, France). The biomass (substrate) was mainly composed
of Scenedesmus (identified microscopically) but also bacteria in
Anaerobic digesters
In order to avoid any misleading effect of water vapour pressure, an open to the atmosphere reactor was used. Two mild
temperatures were tested, namely 70 and 90 C. Temperatures
were achieved by means of a water bath. This water was
b i o m a s s a n d b i o e n e r g y 4 0 ( 2 0 1 2 ) 1 0 5 e1 1 1
2.4.
yields on COD basis ranged 110 and 180 dm3 kg1 for 16 and 23
days HRT [16]. According to these findings, it could be
concluded that Scenedesmus sp. presents a higher resistance to
anaerobic degradation when compared with C. vulgaris.
Indeed, those results are in agreement with data presented by
Mussgnung et al. [14]. Batch anaerobic biodegradability test
carried out by those authors revealed methane production on
VS basis of 178 dm3 kg1 for S. obliquus whereas Chlorella kessleri digestion attained 217 dm3 kg1. The difference on
methane production was attributed to the presence of
sporopollenin-like polymers (hardly biodegradable), hence
hampering organic matter hydrolysis and subsequent
methane conversion.
3.2.
Effect of thermal pretreatment on Scenedesmus sp.
biomass
Analysis
3.
107
3.1.
Methane production of not-pretreated
Scenedesmus sp. biomass under semi-continuous
conditions
Anaerobic digestion of Scenedesmus sp. biomass under semicontinuous conditions resulted in low methane production.
More specifically, methane yield on COD basis was around
84 dm3 kg1 while methane content in the biogas ranged
between 58 and 60%. Based on the methane production it can
be calculated that only 24% of the biomass was converted in
biomass. This fact proved the high resistance of Scenedesmus
sp. to anaerobic bacterial attack. Similar conditions were
employed to anaerobically digest C. vulgaris and methane
108
b i o m a s s a n d b i o e n e r g y 4 0 ( 2 0 1 2 ) 1 0 5 e1 1 1
b i o m a s s a n d b i o e n e r g y 4 0 ( 2 0 1 2 ) 1 0 5 e1 1 1
109
Fig. 2 e Sytox staining Scenedesmus ecosystem of not thermally pretreated biomass (A) and thermally treated at 90 C after
(B) 30 min, (C) 60 min and (D) 180 min.
200
150
100
50
0
0
10
20
30
Digestion time (days)
40
110
b i o m a s s a n d b i o e n e r g y 4 0 ( 2 0 1 2 ) 1 0 5 e1 1 1
4.
Conclusions
Acknowledgements
This research was financially supported by the French
National Research Agency for the Symbiose project (ANR-08BIO-E11). The authors thank Emilie Picon from Naskeo
(France) for the microscopic analysis of Sytox green.
references
b i o m a s s a n d b i o e n e r g y 4 0 ( 2 0 1 2 ) 1 0 5 e1 1 1
111