Beruflich Dokumente
Kultur Dokumente
Results
A) Control
Positive Control
Negative Control
1) Did the reaction give off heat? =The reaction give off heat, but turn
cold immediately because the surrounding temperature was lower.
B) Effect of Temperature on Enzyme Activity
Temperature
(C)
4.0
22.5
37.0
100.0
5
0
2
4
Enzyme Activity
6
5
4
Enzyme Activity
3
2
1
0
4.0C
22.5C
37C
100C
pH 3
pH 7
pH 11
Enzyme Activity
(1-5)
0
5
4
Enzyme Activity
6
5
4
Enzyme Activity
3
2
1
0
pH 3
pH 7
pH 11
Enzyme Activity
(1-5)
4
10%
1%
Description
Highly liver concentrated solution,
vigorous reaction occurred
Medium liver concentrated solution, less
reaction occurred
Low liver concentrated solution, no
reaction occurred
Enzyme Activity
(1-5)
5
Chicken
Apple
Potato
Carrot
3
1
1
1
Description
Rapid effervescence, a lot of bubbles
produced
Turns white, white bubbles produced
Little reaction, color remains the same
Little reaction, slightly to light brown
Little reaction, color remains the same
1) What do your results tell you about the functions of the different types
of tissues?
Different tissues have different amount of various enzymes. Activity of
most enzymes differs from one type tissue to another. As for liver,
with its detoxifying functions, has a higher level of catalase enzyme
compared to chicken tissue, apple tissue, potato tissue and carrot
tissue.
2) Why was it important that the puree of each substance is obtained by
blending the same amount of substance in a similar volume of water?
So that we can make comparison effectively between the level of
reactivity of catalase enzyme in different type of tissues.
Conclusion
s
37C is the best temperature for catalase enzyme activity works optimum,
and pH 7 is another most suitable condition for catalase enzyme to work at
its best. Besides, catalase enzyme will react effectively against hydrogen
peroxide if it is fully presented (100% concentrated). Last but not least,
catalase enzyme activity is higher in liver compared to other different
tissues.
Questions
5) (a) The U.S. Food & Drug Administration recommends that cooked
beef be refrigerated for no more than 3-5 days before it is eaten; for
cooked fish they recommend only 1-2 day(s). Why do you think fish
might not keep as long in the refrigerator?
(b) It is recommended that uncooked ground beef is refrigerated for
7) Lizards and snakes may often be found sitting in sunny spots (on
exposed rocks, in the middle of a road) in the morning. They do not
use the heat generated by their bodies to heat themselves; they
obtain heat from the environment. After a cold night, they are sluggish
and must heat up before they can be active.
(a) What do they need heat for?
They need heat for helping them to metabolize and function as a
living organism.
(b)From where does the body heat from mammals and birds come?
(The Second Law of Thermodynamics might help you answer this
one.)