Beruflich Dokumente
Kultur Dokumente
Abstract
We have used synchrotron X-ray diffraction to study the texture and the change in lattice parameter as a function of position in a cross
section of human dental enamel. Our study is the rst to map changes in preferred orientation and lattice parameter as a function of
position within enamel across a whole tooth section with such high resolution. Synchrotron X-ray diffraction with a micro-focused beam
spot was used to collect two-dimensional (2D) diffraction images at 150 mm spatial resolution over the entire tooth crown. Contour maps
of the texture and lattice parameter distribution of the hydroxyapatite phase were produced from Rietveld renement of diffraction
patterns generated by azimuthally sectioning and integrating the 2D images. The 002 Debye ring showed the largest variation in
intensity. This variation is indicative of preferred orientation. Areas of high crystallite alignment on the tooth cusps match the expected
biting surfaces. Additionally we found a large variation in lattice parameter when travelling from the enamel surface to the enameldentine junction. We believe this to be due to a change in the chemical composition within the tooth. The results provide a new insight on
the texture and lattice parameter proles within enamel.
r 2007 Elsevier Ltd. All rights reserved.
Keywords: Enamel; Hydroxyapatite; Apatite structure; Synchrotron X-ray diffraction; Texture; Preferred orientation
1. Introduction
Dental enamel is the most highly mineralised and
hardest biological tissue. It is comprised of approximately
96% mineral, 3% water, and 1% organic matter (noncollagenous protein) by weight [1]. The mineral is nonstoichiometric calcium hydroxyapatite (Ca10(PO4)6OH2)
with carbonate, uoride, sodium, and magnesium ions
frequently found within the structure. These hydroxyapatite (HA) crystallites are laid down as nanorods with crosssectional dimensions of 50 nm 25 nm and up to 1 mm
long [2]. Clusters of these nanorods, known as prisms,
contain around 1000 crystallites. They are approximately
5 mm in diameter and may be up to several millimetres long,
and the majority are arranged with their long axes at
approximately 901 to the enamel-dentine junction (EDJ).
Corresponding author. Tel.: +441133438331.
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3. Results
3.1. Preferred orientation in enamel
Preferred orientation has both a magnitude and a
direction. Through our analyses of the X-ray diffraction
data we have been able to quantify both these parameters.
In order to obtain an overview of the preferred orientation
in a tooth section the 2D X-ray scans were arranged to
form a composite map of CCD images of the tooth, as
shown in Fig. 3. Each small square in the image is one 2D
diffraction pattern. The centres of adjacent diffraction
patterns are 150 mm apart. The shape of the tooth can
clearly be seen from this composite image. The darker
patterns in the middle of the tooth are from dentine and the
lighter patterns covering these are from the enamel. At the
surface the enamel is thinner, therefore there is a halo of
darker images along the edge of the tooth where there is
partial air scattering. This can also be seen in the ssure
where there is a gap between the two cusps. Additionally, it
can be seen that below the ssure there is a circular region
of enamel which is darker than the surrounding patterns. It
is likely that this is caused by a ssure caries lesion which
has partially demineralised the enamel in that area.
In Figs. 4ad four individual diffraction scans from
different parts of the tooth are shown. Figs. 4a, c and d
illustrate the change in texture direction in the 002 plane at
different positions within the enamel. Variations in
intensity around diffraction rings are indicative of texture
in the tooth enamel. The strongest texture (the most
extreme variation in intensity) was found in the 002
reection (2y 13.71)labelled in Fig. 4a. A line through
zero degree has also been marked in Fig. 4a. Fig. 4b shows
the diffraction pattern from dentine. Here the peaks are
much broader indicating that the crystallites are smaller.
There is also much less variation in intensity around the
Fig. 4. (a), (c) and (d) Illustrate the change in texture direction at
difference positions within the enamel. (b) Shows the poorly crystalline
nature of dentine.
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Fig. 5. Typical intensity versus Azimuthal angle curve for the 002
reection showing the pronounced texture in this sample. The left hand
peak has been tted to a Gaussian.
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open circles are the observed data points, and the solid line
is the calculated diffraction pattern. Below the pattern is a
plot of the difference (observedcalculated), and beneath
are the tick marks for the 2y peak positions for the
calculated diffraction pattern of HA. The difference plot
shows that the agreement between observed and calculated
data is generally very good with a typical value for w2 of
1.5. The nal parameters obtained from this renement
are given in Table 1. Similar renements were carried out
on all diffraction patterns.
A contour map showing the change in magnitude of
preferred orientation in the 002 diffraction peak has been
plotted in Fig. 8. The 002 preferred orientation parameters
have been extracted for the zero degree slice of each 2D
diffraction image (see Fig. 4a). Areas with higher values of
preferred orientation parameter are more strongly textured
i.e. the crystallites are more aligned to the zero degree
direction in these areas. Areas with low texture coefcient
have less well-aligned crystallites.
3.3. Change in lattice parameters
Both the a- and c-lattice parameters of HA were rened
in each diffraction pattern and after carrying out Rietveld
renements of 1095 data sets it was noticed that neither the
a- nor c-lattice parameters were constant as a function of
position. Note that the variation was not due to X-ray
wavelength drift as a function of time, but was clearly
dependent on the position within the tooth. Trends in the
lattice-parameter changes have been plotted in Fig. 9
relative to the average lattice parameters. The relative
percentage change in the a-lattice parameter (Ra) across the
tooth section was calculated using:
an a0
Ra
100%,
a0
Fig. 6. Texture direction of the 002 reection of hydroxyapatite crystallites in enamel, calculated using 2D diffraction images.
Fig. 7. Typical diffraction pattern including the raw data (circles), the calculated diffraction pattern (solid line), the difference, and the tick marks for the
2y peak positions for the calculated diffraction pattern of hydroxyapatite.
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Table 1
Rened structural parameters for typical diffraction pattern of dental enamel (HA)
Phase hydroxyapatite
Space group
a (A)
c (A)
a, b, g
V (A3)
Y(particle)
002(SH)
w2
P63/m (#176)
9.4660(9)
6.9018(3)
a b 901, g 1201
535.49(8)
7.83(9)
1.9(1)
1.5
Y(particle) is the coefcient for Lorentzian particle size broadening, and 002(SH) is the 002 spherical harmonic preferred orientation term.
Fig. 9. (a) a-lattice parameter and (b) c-lattice parameter contour maps
showing the change in lattice parameter value at different positions
around the tooth.
4. Discussion
It has been seen previously that there is a higher degree
of crystallite alignment in surface enamel compared to
enamel close to the EDJ [6]. However, in that work, only
linear slices from EDJ to surface were probed. The results
from our study show that the texture distribution is
much more complex than previously thought. We can see
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Fig. 10. Four tracks through the tooth section going from enamel surface
to EDJ showing the change in lattice parameter as a function of distance
from the enamel surface. The tracks are indicated in Fig. 1.
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