Course:

MSL4010 Certificate IV in Lab Techniques

Unit(s):

MSL924001A Process & Interpret Data

Unit Co-ordinator:

Tina Currie

Assessment:

Assignment 2

Percentage of Unit: 10%

___________________________________________________________________
Student Name: Carolina Gonzalez

Date: 11-05-2015

Student Number: 100523715

Group: B

Instructions:

Type your Name, Student Number, Group and Date in the space above.

Type ALL your solutions into the space provided (create extra space for
required.)

Insert excel charts as indicated.

Round off final answers as specified (dont round off during your calculations).

Once completed submit your Assignment via Moodle.

Total =

37

Holmesglen: TC 28 May 2015 http://online.tafevc.com.au/holmesglen/pluginfile.php/154409/mod_assign/intro/ASSIGNMENT 2.docx

working as

Question 1: 18marks
The following set of data shows the Birth weight of babies (in oz.) and the %increase in their weight 100
days after birth.
Birth Weight
(Oz)
72
112
111
107
119
92
126
80
81
84
115
118
128
128
123
116
125
126
122
126
127
86
142
132
87
123
133
106
103
118
114
94

1.

% Increase in Weight after 100

day of birth
68
63
66
72
52
75
76
118
120
114
29
42
48
50
69
59
27
60
71
88
63
88
53
50
111
59
76
72
90
68
93
91

Which is the independent variable? Which is the dependent variable?

Why?

2 marks

The independent variable is the Birth Weight and the dependent

variable is % Increase in Weight after 100 day of birth, because the
% would be affected if the birth weight changes.
2.

Use excel to create a scatterplot of this data and insert a trendline. Make sure you
include the following:
Axis labels
Chart title
Equation of the trend line
Coefficient of determination (r2)

Holmesglen: TC 28 May 2015 http://online.tafevc.com.au/holmesglen/pluginfile.php/154409/mod_assign/intro/ASSIGNMENT 2.docx

4 marks

Birth Weight vs. % Increase in Weight after 100 day of birth

140
120
100
f(x) = - 0.86x + 167.87
80
R = 0.45
% Increase in Weight after 100 day of birth 60
40
20
0
50 100 150
Birth Weight (Oz)

3.

What does the value of r2 tell you about the reliability of this trendline at making
predictions?

1 mark

The trendline is only 45% reliable at making predictions.

4.

a) Use excel to calculate persons correlation coefficient

(r) and display your answer below. (Round off your
answer to 2 decimal places).

2 marks

r = - 0.67
b) What does this value tell you about the type & strength
of relationship that exists between the two variables?
Negative and medium linear relationship between Birth Weight and
% Increase in Weight after 100 day of birth.

5.

What is the gradient and Y-intercept of the trendline? (Round off your answer
to 3 sig figs).

2 marks

m= -0.864
y= 168
6.

Calculate the x-intercept of the trendline from the equation of the trendline.
Round off your FINAL answer to 3 sig figs.
y = -0.864x + 167.87
0 = -0.864x + 167.87
-167.87 = - 0.864x

x=

167.87
0.864

194.294

x= 194

Holmesglen: TC 28 May 2015 http://online.tafevc.com.au/holmesglen/pluginfile.php/154409/mod_assign/intro/ASSIGNMENT 2.docx

3 marks

7.

Use excel to display the x intercept of your trendline by extrapolating it back to

the x axis.

2 marks

Change the scale on the X axis so that it goes up by smaller units and begins
closer to the original value.

Birth Weight vs. % Increase in Weight after 100 day of birth

140
120
100

f(x) = - 0.86x + 167.87

R = 0.45

80
% Increase in Weight after 100 day of birth

60
40
20
0

Birth Weight (Oz)

8.

a) What is the x intercept according to your chart?

3 marks

The x intercept according to the chart is 194.

b) What does the value of the x-intercept tell us about birth weight in this
case?
When the birth weight is 194 Oz after 100 day of birth its not going to
increase the weight.
c) Would you consider this a reliable prediction? Why?
Im not consider this a reliable prediction, because is only 45% reliable at
making predictions.

Background Information for Question 2:

This question applies scatterplots and trendlines to a calibration method known as Standard Addition Method.

You will learn more about the standard addition method in chemistry, but I have
included a YouTube clip below for optional viewing (you dont have to watch it to
complete the assignment but it is helpful to further your understanding).

https://www.youtube.com/watch?v=eg4A9PHA9Ps

The standard addition method requires the use of Atomic absorption spectrometry
(AAS) to measure the concentration of elements. (You will also learn all about AAS in

Holmesglen: TC 28 May 2015 http://online.tafevc.com.au/holmesglen/pluginfile.php/154409/mod_assign/intro/ASSIGNMENT 2.docx

chemistry). (If you want to better understand AAS I have included a very helpful link
below.)
http://www.ausetute.com.au/aas.html
In brief: Atomic-absorption spectroscopy (AAS) uses the absorption of light to
measure the concentration of gas-phase atoms. Since samples are usually liquids or
solids, the analyte atoms or ions must be vaporized in a flame or graphite furnace. The
atoms absorb ultraviolet or visible light and make transitions to higher electronic energy
levels. The analyte concentration is determined from the amount of absorption.

Question 2: Total 19 marks

Lets say you wish to measure the amount of strontium (Sr) in tooth enamel by using
atomic absorption spectroscopy (AAS).
Solutions with a constant TOTAL volume of 10.0mL contained 0.750mg of dissolved tooth
enamel.
Solutions were made up to 10ml using a variety of volumes of strontium stock solution:
0ml, 1mL, 2mL, 3mL, 4mL and 5mL of 20ppm strontium stock solution (this is the
concentration of Sr).
1.

Calculate the concentration of added Sr in each solution. (Insert answers

Vol(added Sr )
Conc
(
added
Sr
)
=
Conc(Sr stock solution)
in table below)
Vol(total solution)
Standard
(Vol of added Sr)
Original Stock
solution
(no added
Strontium)
1ml
2ml
3ml
4ml
5ml

Concentration of added Sr
(ppm) (X axis)
0 ppm

Absorbance
(Y axis)
28.0

2 ppm
4 ppm
6 ppm
8 ppm
10 ppm

34.3
42.8
51.5
58.6
64.3

2. AAS was used to measure the absorbance values for each solution (as
shown above).
Use excel to create a scatterplot of this data and insert a trendline.
Make sure you include the following:
Axis labels
Chart title
Equation of the trend line
Coefficient of determination (r2)

Holmesglen: TC 28 May 2015 http://online.tafevc.com.au/holmesglen/pluginfile.php/154409/mod_assign/intro/ASSIGNMENT 2.docx

2 marks

4 marks

3.

a) Use excel to calculate persons correlation coefficient (r) and display your
answer below. (Round off your answer to 2 decimal places).

2 marks

r = 1.00
b) What does this value tell you about the type & strength of relationship that
exists between the two variables?
Positive and perfect relationship between Concentration of added Sr & Absorbance
4.

Calculate the x intercept from the equation of the trendline.

(Round off the final answer to 3 sig figs).

3
marks

y = 3.7586x + 27.79
0 = 3.7586x + 27.79
-27.79 = 3.7586x

x=

27.79
3.7586

x= - 7.39
5.

What was the recorded absorbance for the standard solution with no added Sr?
Based on this answer, do you think strontium is still present in this solution? Why?

2
marks

28 ppm.
Yes, because the concentration is in the tooth paste.

6. Display the x intercept of your trendline by extrapolating it back to the x axis. Change
the scale on the X axis to smaller units in order to enable the x intercept to be easily
seen.

Holmesglen: TC 28 May 2015 http://online.tafevc.com.au/holmesglen/pluginfile.php/154409/mod_assign/intro/ASSIGNMENT 2.docx

2
marks

7.

a) What is the x intercept based on your chart? What is the actual x intercept
based on your calculations?
Aprox. - 7.5 ppm based on the chart.
-7.39 ppm based on the calculations.
b) What does the answer for the x-intercept tell us about the concentration of Sr
in the original tooth enamel solution?
The original tooth enamel solution contain Sr.
c) How much Sr (if any) is present in the original stock solution? How did you
work this out?
Contain 7.39 ppm Sr, Because this could be seen in the X intersection.
d) Would you consider this a reliable prediction? Why?
Yes, because the trendline is 99.95% reliable.

Holmesglen: TC 28 May 2015 http://online.tafevc.com.au/holmesglen/pluginfile.php/154409/mod_assign/intro/ASSIGNMENT 2.docx

4
marks