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PERIODONTOLOGY 2000
Prostanoids, including prostaglandins and thromboxane, have a variety of roles in physiological and
pathological conditions including inflammation,
immunological function, ovulation, implantation,
cardiovascular disease, and tumorigenesis (153).
Prostanoids contribute to the signs and symptoms of
acute and chronic inflammation including pain,
fever, swelling, and vasodilatation. The physiological
importance of prostanoids is highlighted by the use
of the cyclooxygenase-inhibiting non-steroidal antiinflammatory drugs in the clinical treatment of disorders. In response to various stimuli, arachidonic
acid released from membrane phospholipids is
metabolized to prostaglandins and thromboxane by
cyclooxygenase. In the early 1990s, two isoforms of
cyclooxygenase, cyclooxygenase-1 and cyclooxygenase-2, were identified (62, 63, 154). Generally,
whereas cyclooxygenase-1 is constitutively expressed
in many tissues and supports the prostaglandin biosynthesis required for maintaining organ and tissue
homeostasis, cyclooxygenase-2 is induced after stimulation with proinflammatory molecules including
interleukin-1, tumor necrosis factor-a, and lipopolysaccharide, and is up-regulated during inflammation.
Traditional non-steroidal anti-inflammatory drugs
such as aspirin, naproxen, and indomethacin inhibit
both cyclooxygenase-1 and cyclooxygenase-2 activities and possess adverse side effects that include
gastrointestinal toxicity. Selective cyclooxygenase-2
inhibitors have been developed with the expectation
of safer and fewer adverse side effects; some of them
have already been used for the clinical treatment of
pain associated with osteoarthritis, rheumatoid
arthritis, and menstruation. Furthermore, recent
molecular and pharmacological analysis has demonstrated that prostanoids exert their biological
actions via specific prostanoid receptors on target
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Prostanoid synthesis
Prostanoids are ubiquitous bioactive lipid molecules
derived from the unsaturated 20-carbon fatty acid
arachidonic acid. Prostanoid synthesis is carried out
in three steps: (i) the mobilization of a fatty
acid substrate, typically arachidonic acid, from
membranous phospholipids, through the action of
phospholipase A2, (ii) the formation of prostaglandin
H2 from arachidonic acid by cyclooxygenase, and
(iii) the conversion of prostaglandin H2 to specific
prostanoids by the action of various prostaglandin
synthases, generating five primary bioactive prostanoids including prostaglandin D2, prostaglandin E2,
prostaglandin F2a, prostaglandin I2 (prostacyclin),
and thromboxane A2 (Fig. 1). These prostanoids act
within tissues and cells via specific G-protein-coupled prostanoid receptors, a family of rhodopsin-like
seven transmembrane spanning receptors. They are
designated EP for prostaglandin E2 receptors and FP,
DP, IP, and TP for prostaglandin F2a, prostaglandin
D2, prostaglandin I2 and thromboxane A2 receptors,
respectively (19, 87).
Since 1971, when Vane (140) demonstrated that the
mechanism for the anti-inflammatory effects of nonsteroidal anti-inflammatory drugs is dependent on
the inhibition of prostaglandin synthesis, numerous
studies have focused on cyclooxygenase to develop
anti-inflammatory drugs. In the early 1990s, it was
revealed that there are two types of cyclooxygenase,
cyclooxygenase-1 and cyclooxygenase-2 (62, 63, 154).
Recently, it has been suggested that there is another
cyclooxygenase protein formed as a splice variant of
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Cyclooxygenase-2
Enzyme expression
Constitutive
Inducible
Character of gene
House-keeping gene
Locus
9q32q33.3
1q25.2q25.3
Size of gene
22 kb
8.3 kb
5-flanking region
Size of RNA
2.8 kb
4.6 kb
Expressing cells
Most cells
Glucocorticoid effect
Inhibition of transcription
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Prostaglandin E2 receptors
Prostaglandin E2 is a major product of cyclooxygenase-initiated arachidonic acid metabolism. Prostaglandin E2 has multiple and at times apparently
opposing functional effects including fever, pain,
vasodilatation, bone resorption, and formation, on a
given target tissue and cell (50, 153). The diverse effects of prostaglandin E2 are now explained by evidence for the existence of multiple prostaglandin E2
receptors (EP receptors) on plasma membranes.
Pharmacological analysis and molecular cloning have
revealed the existence of four EP receptor subtypes,
each coded by distinct genes. These receptors are
designated EP1, EP2, EP3, and EP4. The binding
affinities of prostaglandin E2 to the EP receptors have
the following rank order: EP3 > EP4 >> EP2 > EP1,
with Kd values ranging 100-fold from 0.33 to 25 nM (1).
The EP1 receptor was originally described as a
smooth muscle constrictor. The cloned human EP1
receptor cDNA encodes a 402-amino-acid polypeptide (31). Activation of the EP1 receptor leads to
signals via inositol trisphosphate generation and
increased intracellular Ca2+ levels. The human EP2
receptor cDNA encodes a 358-amino-acid polypeptide (115). Activation of the EP2 receptor leads to
an increase in cyclic AMP levels. EP2 receptors are
selectively activated by butaprost and butaprost
activation is considered diagnostic for their characterization. EP3 receptors have multiple splice variants
generated by alternative splicing of the C-terminal
tail. In humans at least eight EP3 receptor isoforms
have been identified (61), and multiple splice variants exist for other species including mouse, rabbit,
and cow. Originally, the EP3 receptor was described
as coupling to a Gi-type G protein leading to inhibition of intracellular cyclic AMP (130), but subsequently it was shown that individual splice variants
could also couple to enhancement of cyclic AMP and
inositol trisphosphate generation (46, 86). It is true
that important functional differences exist between
the EP3 receptor splice variants in cell culture systems, but the physiological significance of these
different C-terminal splice variants remains unclear.
The EP4 receptor couples to a Gs-type G protein
leading to stimulation of adenylyl cyclase and increased intracellular cyclic AMP levels. The human
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EP4 receptor cDNA encodes a 488-amino acid polypeptide with a predicted molecular mass of 53 kDa
(4). Before 1995, this receptor cDNA was generally
referred to as the EP2 receptor (91). Functional differences in EP2 versus EP4 receptor signaling may
also arise from differential agonist-induced desensitization and internalization. Compared to the EP2
receptor, the EP4 receptor has a much longer C-terminal tail that is required for rapid agonist-induced
desensitization (3) and undergoes agonist-induced
internalization (21).
Recently, the expression of functional EP receptors
(EP1, EP3, and EP4) on the nuclear membranes of
cells has been documented (6, 7). Further studies are
necessary to determine the processes that govern the
expression and function of the nuclear-localized
receptors.
Involvement of cyclooxygenase-2
in prostaglandin E2 production in
periodontal disease
Cavanaugh et al. (13) first performed the immunohistochemical analysis of cyclooxygenase-2 protein
expression in inflamed gingival tissues. They found
that cyclooxygenase-1 and cyclooxygenase-2 proteins
were expressed in fibroblasts, gingival epithelial cells,
endothelial cells, and inflammatory mononuclear
cells. We examined the expression of cyclooxygenase-1
and cyclooxygenase-2 proteins in clinically healthy
and inflamed human gingiva. In both types of
gingiva, cyclooxygenase-1-immunoreactive cells were
detected in subepithelial connective tissue including
fibroblasts and endothelial cells and some gingival
epithelial cells were slightly immunopositive for
cyclooxygenase-1 (Fig. 2A,C). In inflamed gingiva,
inflammatory cells were also immunopositive for
cyclooxygenase-1. However, cyclooxygenase-2 protein was detected in fibroblasts, gingival epithelial
cells, endothelial cells, and inflammatory cells in
inflamed gingiva, whereas in clinically healthy gingiva,
it was detected at low levels in only gingival epithelial
cells and fibroblasts (Fig. 2B,D). Zhang et al. (161) have
demonstrated by quantitative polymerase chain
reaction and Western blot that cyclooxygenase-2
messenger ribonucleic acid and protein levels are
elevated in inflamed gingival tissues from periodontitis patients compared with in uninflamed tissues from
healthy subjects. Furthermore, Morton & DongariBagtzoglou (84) have reported that the levels of cyclooxygenase-2 protein are higher in more inflamed
gingival tissues. Recently, Miyauchi et al. (79) have
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There are several endogenous inhibitors to suppress prostaglandin production in vivo. Glucocorticoid can down-regulate cyclooxygenase-2 expression
to reduce prostaglandin production (74). In addition
to glucocorticoid, interleukin-4, interleukin-10, and
interleukin-13 are well known as anti-inflammatory
cytokines. The cytokines can inhibit the production
of proinflammatory cytokines such as interleukin-1,
interleukin-6, interleukin-8, and tumor necrosis
factor-a by monocytes (16, 38) and suppress bone
resorption (145). Interleukin-4, interleukin-10, and
interleukin-13 can inhibit prostaglandin production
via down-regulation of cyclooxygenase-2 expression
in human monocytes and neutrophils (25, 90, 89). In
human gingival fibroblasts and periodontal ligament
cells, interleukin-4 decreased interleukin-1-induced
prostaglandin production via inhibition of cyclooxygenase-2 expression with no effect on cyclooxygenase-1 expression. Interleukin-13 also inhibits
interleukin-1-induced prostaglandin production via
inhibition of cyclooxygenase-2 expression although it
is less potent compared to interleukin-4 (unpublished data). Recently, it has been shown that
interleukin-4 receptor and interleukin-13 receptor
(interleukin-13 receptor a1 chain) are expressed in
human gingival fibroblasts (64). Interleukin-4, interleukin-10, and interleukin-13 are detected in
inflamed periodontal tissues. Furthermore, interferon-c, a Th1 cytokine, also decreases interleukin-1elicited prostaglandin E2 production in human
gingival fibroblasts and human periodontal ligament cells (39, 95). Hayashi et al. (39) reported that
interferon-c inhibited interleukin-1-induced cyclooxygenase-2 expression, but we could not find the
inhibitory effect of interferon-c on cyclooxygenase-2
expression (95). It is plausible that in periodontal
lesions there are inhibitory systems to regulate
prostaglandin production.
From these results, it is very likely that cyclooxygenase-2 plays a crucial role in producing prostaglandin production in periodontal lesions. As shown
in Fig. 3, there may be stimulatory and inhibitory
systems to regulate prostaglandin production by cell
cell interaction in periodontal lesions.
Fig. 3. Hypothetical regulatory mechanism of cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2)
production through cellcell interaction in periodontal
lesions. As a stimulatory mechanism for PGE2 production
in periodontal lesions, periodontopathic pathogens may
directly activate monocytes/macrophages (M/ and fibroblasts to induce COX-2 expression, resulting in PGE2
production, or may stimulate monocytes/macrophages
Methods/results
The effect of meloxicam on bone loss in ligature-induced periodontitis in rats and its
post-treatment effect after administration withdrawal. Meloxicam reduced bone loss.
No remaining effect was expected after its withdrawal.
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Effects of prostaglandin E2 on
immune and inflammatory
responses
It has been suggested that prostaglandin E2 plays an
important role at multiple levels within the immune
system. Prostaglandin E2 induces T-cell proliferation
via inhibition of polyamine synthesis, intracellular
calcium release or the activity of p59 protein tyrosine
kinase (17, 18, 117). Prostaglandin E2 causes apoptosis of T cells, depending on the maturation state of
the cells.
Interleukin-12 plays critical roles in the induction
of T helper type 1 (Th1) responses by regulating the
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Fig. 4. Prostaglandin E2 (PGE2) inhibits lipopolysaccharide (LPS)-induced tumor necrosis factor-a (TNF-a) production by human monocytes. (A) Human peripheral
blood monocytes were stimulated with buffer, 1 lg/ml
Actinobacillus actinomycetemcomitans ( A.a.) LPS alone
or A.a LPS + indicated doses of PGE2 in the presence of
1 lM of indomethacin. After 48-h incubation, TNF-a levels
in the culture media were measured by enzyme-linked
as that between lymphocytes and gingival fibroblasts (85). It was shown that prostaglandin E2
down-regulated interleukin-1b-, tumor necrosis factor-a- and lipopolysaccharide-induced intercellular
adhesion molecule-1 expression via EP2/EP4 receptors in human gingival fibroblasts (94, 98, 100).
Interleukin-6 is a pleiotropic cytokine, which
induces B-cell activation and osteoclast formation.
Interleukin-1 can induce interleukin-6 production in
human gingival fibroblasts. Prostaglandin E2 suppresses interleukin-1-induced interleukin-6 production in human gingival fibroblasts derived from
periodontally healthy subjects, but increases interleukin-1-induced interleukin-6 production in human
gingival fibroblasts derived from periodontitis
patients (20, 133), To explain the differential
regulation by prostaglandin E2, we showed that EP1
receptor activation causes an increase in interleukin1-induced interleukin-6 production while EP2/EP4
receptor activation leads to a decrease (101). The
differential regulation of matrix metalloproteinase-3
production by prostaglandin E2 in healthy subjects
and periodontitis patients was also reported (118).
Therefore, the difference of expression and functions
of EP receptors may be involved in the regulation of
host responses.
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Conclusions
It is clear that prostaglandins are involved in the
pathogenesis of periodontal diseases because a lot
of studies have indicated that in both animal
and human models traditional non-steroidal
anti-inflammatory drugs inhibit progression of the
diseases. According to recent researches, cyclooxygenase-2 plays a crucial role in prostaglandin
production in periodontal disease and selective
cyclooxygenase-2 inhibitors are as efficacious as traditional non-steroidal anti-inflammatory drugs for
the inhibition of progression of periodontal disease in
animal models. Therefore, cyclooxygenase-2 inhibitors may be effective for host modulatory therapy,
but careful clinical studies are necessary to prove it,
based on the understanding of the advantages and
disadvantages of cyclooxygenase-2 inhibitors. It has
been shown that cyclooxygenase-2 may be proinflammatory during the early phase of a carrageenaninduced pleurisy, dominated by polymorphonuclear
leukocytes, but may aid resolution at the later,
mononuclear cell-dominated phase by generating an
alternative set of anti-inflammatory prostaglandins
including prostaglandin D2 and 15-deoxyD12,14 prostaglandin J2 (33). To better understand the roles of
prostaglandins in periodontal diseases, further studies about the effects of not only prostaglandin E2 but
also other prostaglandins including prostaglandin
F2a, prostaglandin I2, thromboxane A2, and prostaglandin D2 should be performed. As discussed above,
prostaglandin E2 has proinflammatory and antiinflammatory effects, depending on the receptors
used. Therefore, the precise roles of prostaglandins in
physiologic and pathologic conditions should be
determined by an intricate set of ligandreceptor
interactions that depend on multiple factors such as
ligand affinity, receptor expression profile, differential coupling to signal transduction pathways, and
95
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