plasma darah berada di atas lapisan tersebut[2] dengan kepadatan sekitar 1025 kg/m3, or

1.025 kg/l
Whole Blood vs. Plasma and Serum
Plasma differs from whole blood in that the cellular material has been removed by
centrifugation. Plasma is typically about 55% of the blood volume. Blood is drawn into a
collection tube that contains an anti-coagulant (EDTA or citrate) and placed in a centrifuge to
separate the cells from the plasma. The plasma contains the dissolved proteins, clotting
factors, and other suspended materials in water. Plasma viscosity is tested using a rotating or
capillary viscometer using the separated plasma only.
Serum is similar to plasma, except that the blood is drawn into a collection tube without anticoagulant and allowed to clot for about 20 minutes. Then, after coagulation, the specimen is
centrifuged to separate the serum from the clotted blood. Serum viscosity is tested using a
rotating or capillary viscometer and is marginally lower than plasma because the clotting
factors have been removed.
Plasma and serum viscosity tests are useful to detect an acute phase reaction or severe
inflammatory response such as occurs in sepsis. Plasma and serum viscosity is also used in a
number of clinical diagnoses such as Waldenstrm macroglobulinemia. However, because the
cellular content of blood has been removed prior to testing viscosity, plasma and serum
cannot provide any insights on the actual flow resistance of a patients blood sample. In
particular, the red blood cells, their proportion to plasma volume, and the deformability
(flexibility) of the red blood cells have a profound influence on actual blood viscosity and flow
resistance. Whole blood viscosity is the ability of the blood to flow, which determines the
burden of work to the heart in pumping blood, the physical injury that blood can cause
against the inner lining of the arteries, and the delivery of oxygen to the organ systems. In
order to quantify the actual flow resistance of blood, it is necessary to measure whole blood
viscosity.

Newtonian vs. Non-Newtonian

Whole blood behaves as a non-Newtonian fluid, which means that its viscosity changes as a
function of shear rate. Shear rate is the velocity divided by the vessel diameter. For
convenience sake, we can think of shear rate simply as blood flow velocity. When blood
moves quickly as in peak-systole, it is thinner. When it moves slowly during end-diastole it is
thicker and stickier. This is because the red cells aggregate. The phenomenon is known as the
shear-thinning, non-Newtonian behavior of whole blood [1].
Serum and plasma are Newtonian fluids like water, which means that no matter how fast
these fluids flow their viscosity does not changes (see the figure below).
Inside the body, blood experiences high and low shear rates during each cardiac cyclefrom
peak-systole (high shear) to end-diastole (low shear). The viscosity of blood is changing
dynamically during each cardiac cycle because the red blood cells aggregate during diastole.

Because the blood cells are removed prior to testing, plasma and serum viscosity
measurements cannot provide data that are physiologically meaningful and fail to capture the
dynamic changes of blood flowing in the body.
The Complete Blood Viscosity Profile
Meridian Valley Laboratory uses an automated scanning capillary viscometer that acquires the
complete viscosity profile for each blood sample tested. Systolic blood viscosity (high shear)
and diastolic blood viscosity (low shear) are reported for easy clinical intepretation. A few
reference labs in the U.S. do test the viscosity of whole blood but are only able to measure
high shear viscosity (at a shear rate of 90 s-1). This is like testing blood pressure with a cuff
and only being able to measure a single value for systolic blood pressure, instead of both
systolic and diastolic. Meridian Valley Laboratory is proud to be the only provider delivering a
complete blood viscosity profile for each blood specimen received at our lab.