Beruflich Dokumente
Kultur Dokumente
DOI 10.1007/s13197-012-0648-5
ORIGINAL ARTICLE
Introduction
Free radicals are types of Reactive oxygen species (ROS),
which include all highly reactive, oxygen-containing molecules. Types of ROS include the hydroxyl radical, the super
oxide anion radical, hydrogen peroxide, singlet oxygen,
nitric oxide radical, hypochlorite radical, and various lipid
peroxides. All these are capable of reacting with membrane
lipids, nucleic acids, proteins and enzymes and other small
molecules, resulting in cellular damage (Shahidi and
Wanasundara 1992; Buyukokuroglu et al. 2001).Cell damage
caused by free radicals appears to be a major contributor to
aging and degenerative diseases of aging such as cancer,
cardiovascular disease (Halliwell and Gutteridge 1984; Ames
et al. 1993; Harman 1995; Halliwell 1997), cataract, immune
system decline, liver diseases, diabetes mellitus, inflammation,
renal failure, brain dysfunction, etc. (Miller 1996; Halliwell
1997; Kowaltowski and Vercesi1999). Antioxidants are absolutely critical for maintaining optimal cellular and systemic health and well-being.
Coriandrum sativum L. (coriander) is an annual herb
belonging to the Apiaceae (Umbellifera) family (Evans et
al. 2002). Different parts of the plant, including the fruits
and the green herbs, are used for medicinal purposes such as
dyspeptic complaints and loss of appetite (Blumenthal et al.
2000). Pharmacological studies in animals have shown that
coriander has anti-diabetic (Swanston-Flatt et al. 1990; Gray
and Flatt 1999), hypolipidemic, (Chithra and Leelamma
Estimation of total flavonoid content Total flavonoid content was determined as described by Zou et al. (2004).
0.25 ml of various extracts was diluted with 1.25 ml of
distilled water. 75 l of a 5% NaNO2 solution was added
followed by 150 l of a 10% AlCl3.H2O after 6 min. After
5 min, 0.5 ml of 1 M NaOH was added to this mixture. The
absorbance was measured immediately against the prepared
blank at 510 nm. Catechin was used as a standard and the
results were expressed as mg of catechin equivalents (CE)
per mg of dry extract.
ABTS radical cation scavenging activity The ABTS (2, 2azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) cation
radical scavenging activity was performed with slight modifications described by Roberta et al. (1999). The ABTS+
radicals were produced by the reaction between 7 mM
ABTS in water and 2.45 mM potassium persulphate, stored
in the dark at room temperature for 12 h. Prior to use, the
solution was diluted with ethanol to get an absorbance of
0.7000.025 at 734 nm. Free radical scavenging activity
was assessed by mixing 10 l of test sample with 1.0 ml of
liver homogenate. A 10% (w/v) rat liver sample homogenate was prepared with homogenizer at 04 C with 0.15 M
KCl. The homogenate was centrifuged at 900 x g for
15 min, and clear cell-free supernatant was used for the
study with in vitro lipid peroxidation assay. Different concentrations (01000 g/ml) of extract and standard BHA 0
100 g/ml were added in test tubes and 1 ml of 0.15 M KCl
followed by 0.5 ml of rat liver homogenates were added.
Peroxidation was initiated by adding 1000 l of 200 M
AAPH. After incubation at 37 C for 30 min, the reaction
was stopped by adding 15% TCA, 0.38% TBA, and 0.5%
BHT. The reaction mixtures were heated at 80 C for
60 min. The samples were cooled and centrifuged and the
absorbance of the supernatant was measured at 532 nm.
Calculation The percentage inhibition for all the above
methods was calculated according to the formula:
A0 A1 =A0 100;
Where A0 was the absorbance of the control and A1 was the
absorbance of the sample.
IC50 value was determined from the plotted graph of
scavenging activity using the formula y0m x+c against
the different concentrations of extracts of Coriandrum sativum L. which is defined as the total antioxidant necessary
to inhibit radicals by 50%. The measurements were triplicated and their scavenging effect was calculated based on
the percentage inhibition.
Statistical analysis All assays were carried out in triplicates.
IC50 values were calculated using regression equation in
excel programme.
Conclusion
In order to characterize antioxidant activity of a plant extract, it is desirable to subject it for the tests that evaluate the
range of activities such as scavenging of the reactive oxygen
species, metal ion chelation; inhibition of lipid peroxidation
and DNA damage protection assay. This study demonstrated
a concentration dependent free radical scavenging activity.
As part of our ongoing research in to the phytochemistry
and antioxidant properties of herbs, spices and plant-derived
medicinal preparations, we have investigated a hydroalcohol extract of Coriandrum sativum leaf of India. Linalool, a water soluble monoterpene may be one of the phytochemical, responsible for the antioxidant property. However,
further studies are warranted to ascertain its significant role.
This study indicated the potential of the leaf extract as a
source of natural antioxidants or nutraceuticals that could be
of use in food industry with potential application to reduce
oxidative stress in living system with consequent health
benefits.
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