Lahore University of Management Sciences

Fundamentals of Molecular Techniques (BIO511)

Fall 2013
INSTRUCTORS
Room No.
Office Hours
Email
Telephone
Secretary/TA
TA Office Hours
Course URL (if any)

Drs. Sohail A. Qureshi, Zakir Ullah, Muhammad Tariq

Room 10-507 (SAQ); 9-413A (ZU); 9-415A (MT)
TBA
Sohail.qureshi@lums.edu.pk; Zakirullah@lums.edu.pk; m.tariq@lums.edu.pk
X-8349 (SAQ); X-8217 (ZU); X-8218 (MT)
Haris Manzoor (SAQ); Rana Sarfraz Ilyas (ZU & MT)
NA
None

COURSE BASICS
Credit Hours
Lecture(s)
Schedule
Recitation/Lab (per week)
Tutorial (per week)

Three (3)
Nbr of Lec(s) Per Week
Tuesdays & Thursdays
Nbr of Lec(s) Per Week
Nbr of Lec(s) Per Week

COURSE DISTRIBUTION
Core
Elective
Open for Student Category
Close for Student Category

Yes (for graduate students)

Yes (for undergraduates)
Biology majors and minors
Others

Two (2)
Time: 11:00 12:15
NA
NA

Duration
Venue
Duration
Duration

75 minutes
SDSB 102

COURSE DESCRIPTION
Over the past two decades a number of cutting-edge technologies have been introduced which permit investigators to study cellular processes
and function. As a result there are numerous tools available to modern day molecular and cell biologists who are interested in studying genes,
proteins as well as cell and organismal biology. This course has been designed to cover the modern experimental aspects of molecular and cellular
biology.

COURSE PREREQUISITE(S)
Students enrolled in this course are expected to have taken an introductory level course in molecular biology and a firm grasp of
structure and function of nucleic acids and proteins.

COURSE OBJECTIVES
The objective of this course is to impart theoretical as well as experimental aspects of various molecular and cell biology
techniques to students with emphasis on how to design experiments and interpret data.

Lahore University of Management Sciences

Learning Outcomes
After taking this course students should be:

Knowledgeable about methods used for manipulating genes, and analyzing proteins and nucleic acids

Knowledgeable about the various approaches and instruments used for studying cells

Able to understand how experiments are designed and how their results interpreted

Able to critically evaluate raw experimental data and draw conclusions

Capable of troubleshooting technical problems

Grading Breakup and Policy
Midterm Examination: 40%
Project: 20%
Final Examination: 40%

Examination Detail
Yes/No: Yes
Combine Separate: Combined
Midterm
Duration: 3 hours
Exam
Preferred Date: None
Exam Specifications: Short answer questions
Yes/No: Yes
Combine Separate: Combined
Duration: 3 hours
Final Exam
Exam Specifications: Short answer questions

WEEKS
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6

Week 7
Week 8
Week 9
Week 10

Week 11
Week 12
Week 13
Week 14

TOPICS
DNA isolation
Electrophoresis
Polymerase chain reaction
Restriction & Modifying enzymes; DNA Cloning
Recombinant protein production and
purification
Radiolabeling of nucleic acids
DNA sequencing
Detection and quantification of RNA
Analysis of DNA
Analysis of proteins
Generation of monoclonal and polyclonal
antibodies
Culturing cells and organs
Transient and stable transfections
Reporter genes and assays
Stem cells - purification
Generation of induced pluripotent stem cells
Transgenic animals
Methods of studying gene expression
Protein-protein interactions
Protein-DNA interactions
Studying enzymatic activity in vivo and in
vitro activity
Methods for studying signaling pathways
Methods for studying epigenetics
Identification of disease causing genes
Recent advances in gene therapy

INSTRUCTOR(S)
MT; SAQ
SAQ
SAQ
SAQ

SAQ
SAQ

ZA

ZA
MT
SAQ

SAQ
MT
SAQ
SAQ