Beruflich Dokumente
Kultur Dokumente
Dr Debasis Biswas
Lattice formation
Precipitation Reaction
Prozone Zone
Postzone
Zone phenomenon
Precipitation Reaction
Soluble Antigen
Soluble Antibody
Equivalent Proportions
Electrolytes
Temperature
pH
Soluble Antigen
Soluble Antibody
Equivalent Proportions
Electrolytes
Temperature
pH
Antigen- Antibody
Complex Precipitates
Agglutination Reaction
Polyclonal Antibody
Monoclonal Antibody
Particulate Antigen
Soluble Antibody
Equivalent Proportions
Electrolytes
Temperature
pH
Antigen- Antibody
Complex forms Clumps
Immunodiffusion Reaction
Antigen- Antibody
Complex Precipitates
Radial Immunodiffusion
(Mancini)
Antibody incorporated in gel
Antigen diffuses from well
Flocculation Reaction
Soluble Antigen (low sp gr)
Soluble Antibody
Equivalent Proportions
Electrolytes
Temperature
pH
Antigen- Antibody
Complex Floats
11/26/2014
Immunodiffusion Reaction
Immunoelectrophoresis
Double Immunodiffusion
(Ouchterlony)
Antibody & Antigen
diffuse towards each other
Countercurrent Immunoelectrophoresis
Quiz
Rocket
Electrophoresis
Which antigen, H or M, has evoked more antibody formation?
Why?
Agglutination Reaction
Particulate Antigen
Soluble Antibody
Equivalent Proportions
Electrolytes
Temperature
pH
+ +
Antigen- Antibody
Complex forms Clumps
RBC suspension +
S e r i a l D i l u t i o n o f a n t i b o dy
Variants
Latex Agglutination
Hemagglutination
Haemagglutination Reaction
NC: No antibody.
Button formation
Slide Agglutination
Tube Agglutination
11/26/2014
Haemagglutination Inhibition
Coombs Test
Paul Bunnel
Reaction
Cold Agglutinin
Reaction
Streptococcus MG
Agglutination Test
Proteus vulgaris
OX2 OX19
Proteus mirabilis
OXK
Incomplete Antibodies:
Bind to particulate antigen
Cannot agglutinate them
Agglutination effected by adding Ab to the incomplete ab
Direct: Infant
Indirect: Mother
ELISA
11/26/2014
ELISA
Immunofluorescence
Direct IF
Indirect IF
FITC- labelled
Anti- Ig
FITC- labelled Ab
to ag of interest
Incubate antibody
with sample
containing antigen
to be measured
Ab to ag
of interest
Add enzymeConjugated
Secondary
Antibody
Immunofluorescence
Western Blot
Indirect IF
Direct IF
Western Blot
Immunochromatography
Y
: Analyte of interest
: Colloidal gold particles coated with antibody to analyte of interest
: Antibody to analyte of interest
: Antibody to antibody coated on gold particles
T
C
Y
Reaction at T band
Reaction at C band