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There are two basic types of epithelial tissues: covering and lining epithelia and glandular
epithelia
Lining epithelial cells form a continuous layer over all the free surfaces of the body:
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The walls and the organs of the closed ventral body cavities.
General Features:
Derived from all embryonic germ layers, including endoderm, mesoderm and
ectoderm
Cilia: are membrane-covered extensions of the entire apical surface. They beat in
waves, often moving a surface coat of mucus and trapped materials. Ciliated epithelia
include ciliated pseudostratified columnar (respiratory) epithelium and the ciliated
simple columnar epithelium of the oviducts.
Flagella: are also concerned with movement. Spermatozoa, derived from seminiferous
epithelia, are the only flagellated human cells.
Stereocilia: are not true cilia but very long microvilli. They are found in the male
reproductive tract (epididymis. ductus deferens). where they have an absorptive
function, and in the internal ear (hair cells of the maculae and organ of Corti). where
they have a sensory function.
2- Lateral specializations (junctions and folding): cells attach tightly to one another by
specialized intercellular junctions. Several types of junctions can be seen, such as:o Zonula occludens (tight junctions, occluding junctions): are located near the cell apex
and seal off the intercellular space, allowing the epithelium to isolate certain body
compartments (they help keep intestinal bacteria and toxins out of the bloodstream).
Their structure, best seen in freeze-fracture preparations, results from the fusion of 2
trilaminar plasma membranes of adjacent cells to form a pentalaminar structure; this
fusion may require specific "tight-junction proteins." In some tissues, tight junctions
can be disrupted by removing calcium ions or treating with protease.
o Zonula adherens (sometimes called belt desmosomes): are usually just basal to the
tight junctions. The membranes of the adhering cells are typically 20-90 nm apart at a
zonula adherens. An electron-dense plaque containing myosin, tropomyosin, alpha
actinin, and vinculin is found on the cytoplasmic surface of each of the membranes
participating in the junction. Actin containing microfilaments arising from each cell's
terminal web insert into the plaques and appear to stabilize the junction.
o Macula adherens or desmosome, consists of 2 dense, granular attachment plaques
composed of several proteins and borne on the cytoplasmic surfaces of the opposing
cell membranes. Desmosomes, distributed in patches along the lateral membranes of
most epithelial cells. Transverse thin EM sections show dense arrays of tonofilaments
(cytokeratin intermediate filaments) that insert into the plaques or make hairpin turns
and return to the cytoplasm. The gap between the attached membranes is often over 30
nm. Sometimes fibrillar or granular material (probably glycoprotein) is seen as a dense
central line in the intercellular space
o Junctional complex: combination of zonula occludens, zonula adherens and
desmosomes.
o Communicating junctions (gap or nexus junctions): is a disk- or patch-shaped
structure, best appreciated by viewing both freeze-fracture and transverse thin EM
sections. The intercellular gap is 2 nm, and the membrane on each side contains a
circular patch of connexons, the connexons in one membrane link with those in the
other to form continuous pores that bridge the intercellular gap, allowing passage of
ions and small molecules (<800 daltons). As sites of electrotonic coupling (reduced
resistance to ion flow), gap junctions are important in intercellular communication and
coordination; they are found in most tissues.
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3- Basal specialization The basal surface contacts the basal lamina. Because it is the surface
closest to the underlying blood supply, it often contains receptors for blood borne factors
such as hormones.
1. A basal lamina underlies all true epithelial tissues. The basal lamina is a sheet-like
structure, usually composed of type IV collagen, proteoglycan, and laminin, a
glycoprotein that aids in binding cells to the basal lamina. The basal lamina exhibits
electron-lucent and electron-dense layers termed the lamina lucida (lamina rara) and
the lamina dense, respectively. Basal lamina components are contributed by the
epithelial cells, the underlying connective tissue cells, and (in some locations) muscle,
adipose, and Schwann cells. In some sites, a layer of type III collagen fibers (reticular
fibers), produced by the connective tissue cells and termed the reticular lamina,
underlies the basal lamina. Basal laminae accompanied by reticular laminae are often
thick enough to be seen with the light microscope as PAS- positive layers and are
sometimes termed basement membranes. The basal lamina forms a sieve-like barrier
between the epithelium and connective tissue. It aids in tissue organization and cell
adhesion and (through trans membrane linkages with cytoskeletal components) helps
maintain cell shape. it has a role in maintaining specific cell functions, probably
through its effect on shape. Muscle basal laminae are critical in establishing
neuromuscular junctions.
2. Hemidesmosomes: are located on the inner surface of basal plasma membranes in
contact with the basal lamina. They help to attach epithelial cells to the basal lamina.
The best examples are found in the basal layers of stratified squamous epithelium.
3. Sodium-potassium ATPase is a plasma membrane-bound enzyme localized
preferentially in the basal and basolateral regions of epithelial cells. It transports
sodium out of and potassium into the cell.
4- Intracellular Polarity: The nucleus and organelles are often found in characteristic
regions of epithelial cells, a feature particularly important to glandular cells. For example,
in protein secreting cells, the RER is preferentially located in the basal cytoplasm, the
nucleus in the basal to middle region just above the RER, and the Golgi complex just
above the nucleus. Mature secretory vesicles collect in the apical cytoplasm.
Cell polarity: the upper, lower and lateral surface modification of epithelium.
Functions of epithelium
1. Protection: Epithelia protect underlying tissues against physical damage, drying out,
chemical injury and infection.
2. Epithelia allow and regulate the passage of materials (diffusion, absorption, filtration,
secretion, excretion) into and out of the tissues of the body which they cover or line.
3. Sensory reception: Specialized epithelia form sensory parts of organs such as the eye,
ear, mouth (taste buds), and nose (olfactory epithelium).
4. Most glands are derived from epithelial cells specialized for producing secretions.
The classification of the surface epithelia is based on:
1. The number of cell layers
- Simple epithelium: Being composed of one layer of cells only, they are very thin.
They are found in areas of minimum wear and tear. Their main function is to allow
passage of substances between the lumen and the surrounding tissues.
- Stratified epithelium: Being composed of several layers of cells, they are very
thick. Their main function is to protect the tissues that they cover. The shape of the
cells closest to the basement membrane is quite different from that of the cells at the
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top, near the lumen. The stratified epithelia are further classified according to the
shape of the cells at the free surface.
Most often, it is very hard to distinguish the cell's boundary by light microscope. To figure out
what type of cells you are dealing with, look at the shape and position of the nuclei:
If the nuclei are flat and parallel to the free surface: squamous epithelium,
If the nuclei are oval and parallel to the axis of the cell and situated at its base:
columnar epithelium,
If the nuclei are round and situated in the middle of the cell: cuboidal epithelium.
- Transitional: urinary tract; accommodates stretching and toxicity of urine; surface cells
larger, pale-staining, scalloped surface outline; luminal surface appears thickened; may
be binucleate; large, round, prominent nucleoli.
Striated ducts: striations due to mitochondria lined up along folds of basal membrane;
transport Na and bicarbonate; cells high cuboidal to columnar
Myoepithelial cells: Many exocrine glands have specialized type of epithelial cells embedded
in basement membrane between basal lamina and the basal part of secretory cells; may aid
secretion of acinus glands
Basement membranes (external lamina) is formed of 3 layers; lamina lucid, lamina densa
and lamina fibroreticularis. The first two layers (are produced by the epithelia)
Neuro-epithelium: special type of epithelium that has sensory functions. It is found in;
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2. Cells that transport by pinocytosis. Epithelial cells specialized for pinocytosis have
tight junctions and abundant pinocytotic vesicles. The vesicles transport substances across
the cell from the luminal surface to the basal surface or vice versa. The best example is the
endothelial cells lining the blood vessels, where trans-cellular transport is rapid (2-3
minutes).
B. Epithelial Cells Specialized for Absorption:
Specialized absorptive cells lining the digestive tract (especially the small intestine) have
numerous microvilli on their apical surfaces to increase the exposed area. Small nutrient
molecules diffuse into the microvilli, and contraction of the microfilaments shortens the
microvilli, bringing the nutrients into the cytoplasm. Other nutrients are pinocytosed between
microvilli. Absorptive cells with similar specializations occur in the proximal tubules of the
kidney.
C. Epithelial Cells Specialized for Secretion:
1. Protein-secreting cells. Cells that synthesize proteins for segregation and secretion have
abundant basophilic RER, a well-developed Golgi complex, and, frequently, an
accumulation of secretory granules in the cell apex. Proteins secreted by epithelial cells
include the digestive enzymes, produced by pancreatic acinar cells and the chief cells of
the stomach: serum albumin, produced by liver hepatocytes; and protein hormones (eg,
parathyroid hormone, produced by the chief cells of the parathyroid gland).
2. Polypeptide-secreting cells. Secreted polypeptides have fewer amino acids than the
secreted proteins. Polypeptide-secreting cells have a small amount of RER, a supra-nuclear
Golgi complex, and an accumulation of secretory granules in their bases.
3. Mucous cells occur as unicellular, sheet, or solid glands. Histologic features include a
light staining, foamy appearance caused by numerous large mucus-containing vesicles
concentrated near the cell apex. PAS-positive staining from an abundance of
oligosaccharide residues, predominantly acidophilic staining with H&E; a large
supranuclear Golgi complex and sparse RER in the base of the cell.
4. Serous cells have characteristics of protein-secreting cells. They are usually smaller,
darker staining, and more basophilic than mucus-secreting cells. Serous cells include
pancreatic acinar cells and secretory cells of the parotid salivary glands.
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