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IS 15740 (2007): OIL OF ROLSE [PCD 18: Natural and

Synthetic Fragrance Materials]

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IS 15740:2007

,.

Indian Standard
OIL OF ROSE SPECIFICATION

ICS 71.100.60

@BIS2007

BUREAU

OF

INDIAN

STANDARDS

MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG

NEW DELHI 110002
January 2007

Price Group 2

Natural and Synthetic Fragrance Materials Sectional Committee, PCD 18

FOREWORD
This Indian Standard was adopted by the Bureau of Indian Standards, after the draft finalized by the Natural and
Synthetic Fragrance Materials Sectional Committee had been approved by the Petroleum, Coal and Related Products
Division Council.
Rose plant is grown in Bulgaria, Turkey, Morocco, USSR, Spain, Germany, France, Iran and India. In India, the main
areas of its cultivation are located chiefly in Uttar Pradesh, Rajasthan and to some extent in Punjab. Alkaline saline soils
with pH range of 8-9 are quite suitable for the growth of rose plant. This plant flowers during the month of March and
April in North Indian Plains and May and June in hilly areas. Rose oil is used extensively in flowering, perfumery and
medicinal purposes.
In the formulation of this standard, considerable assistance has been derived ffom testing experience of fragrance and
flavour industries.
An International Standard ISO 9842:2003 Oil of rose (Rosa damuscena Miller) also exists, which is not equivalent to
Indian variety.
For the purpose of deciding whether a particular requirement of this standard is complied with the final value, observed
or calculated, expressing the result of a test or analysis shall be rounded off in accordance with IS 2: 1960 Rules for
rounding off numerical values (revised). The number of significant places retained in the rounded off value shoulp be
the same as that of the specified value in this standard.

u!!!!!
IS 15740:2007

Indian Standard
OIL OF ROSE SPECIFICATION

1 SCOPE
This standard specifies the requirements and the methods
of sampling of essential oil of rose (Rosa damascena
Miller),
2 REFERENCES

Chromatography (see Annex A). In the chromatogram

obtained, the representative
and characteristic
components shown in Table 2 shall be identified. The
proportions of these components indicated by the
integrator shall be as shown in Table 2. This constitutes
the chromatographic profile of the essential oil.

The following standards are necessary adjuncts to this

standard:

5.1 Packing

Title

IS No.
326

5 PACKING AND MARKING

The material shall be supplied in airtight containers,

preferably glass, tin-lined or aluminum, permitting a
minimum of airspace, as agreed to between the purchaser
and the supplier.

Methods of sampling and test for

natural and synthetic perfumery
materials:

(Part1):1984 Sampling(second revision)

(Part2) :1980

Preliminary examination of perfhmery

materials
and samples (second
revision)

5.1.1 Direct contact of the material with the galvanized or

mild steel containers shall be avoided.
5,1.2 The material shall be protected from light and stored
in a cool and dry place.

(Part 3): 2006/ Determination of relative density (third

1S0279 :1998 revision)
(Part4): 2005/ Determination of optical rotation (third
ISO 592:1998 revision)

5.2 Marking
Each container so packed shall bear legibly and indelibly
the following information:
a) Name of the material;
b) Manufacturers name and address and/or his
recognized trade-mark, if any;
c) Net and gross mass of the material;
d) Indication of source of manufacture;
e) Batch number;
f) Month and year of manufacture; and
g) Cautionary notice, if any.

(Part 5): 2006/ Determination ofrefiactive index (third

ISO280:1998 revision)
(Part 18): 1984/ Determination of fieezingpoint (second
ISO 1041:1973 revision)
(Part 19): 1998 Gas chromatographic
perfimery materials

analysis

of

1070:1992

Reagent grade water (third revision)

2284:1988

Method for olfactory assessment of

natural and synthetic perfumery
materials (first revision)

6597:2001

Glossary of terms relating to flagrance

and fiavotir industry (second revision)

5.3 BIS Certification Ma,rking

The containers may also be marked with the Standard
Mark.

3 TERMINOLOGY

5.3.1 The use of the Standard Mark is governed by the

provisions of the Bureau,of Indian Standards Act, 1986
and the Rules and Regulations made thereunder. The
details of conditions under which the licence for the use
of the Standard Mark may be granted to manufacturer or
producers may be obtained from the Bureau of Indian
Standards.

For the purpose of this standard, the definitions given in

IS 6597 shall apply.
4 REQUIREMENTS
4.1 The material shall comply with the requirements
given in Table 1.

6 SAMPLING

4.2 Chromatographic Profile

Analysis of the essential oil shall be carried out by Gas

Representative samples of the materials, each sample

1

E
IS 15740:2007
f,,

Table 1 Requirements for Oil of Rose

(Clauses 4.1 and 7.1)
SI No.

Characteristic

Requirements
(3)

Method of Test,
Ref to 1S
(4)

Appearance

Mobile and clear liquid

326 (Part 2)

ii)

Ccdour

Colorless

326 (Part 2)

Iii)

Odour

Characteristic

iv)

Relative

(1)
1)

(2)

v)

VI)
vii)
viii)

to light yellow
rosy

density l]:

a) at 20C

0.8700

to 0.8800

b) at 27C

0.8500

to 0.8750

Refractive

326 (Part 3)

indexll

a) at 20C

1,4520

to 1.4680

b) at 27C

1.4560

to 1.4700

Optical

rotation

Congealing

326 (Part 4)

9 to 12

20 to

point, C

density is synonymous

326 (Part 5)

2 to -4.5

Freezing point, C

II Re[atlve

2284

with specific

degree Celcius change in temperature

gravity.

are 0.00064

containing not less than 50 ml shall be drawn as prescribed

is [S326 (Part 1).

for relative density and refractive index for each

factors

respectively,

water (see IS 1070) shall be employed in tests.

NOTE

Pure chemicals

contain impurities,
7

18)

30

The correction

and 0.00038

326 (Part

shall mean chemicals

which affects the result of

that do not

analysis.

NUMBER OF TESTS

7.1 Test for the determination of all the characteristics

shall be conducted on the composite sample as prescribed
in 4.1,4.2, Table I and Table 2.
7.2

Test Methods

7.2.1 Tests shall be carried out as per

Table 2 Chromatographic Profile

(Clauses 4.2 and7. 1)
S1 No.

Components

Minimum,

Maximum,

Percent

Percent

(3)

(4)

l-Citronellol

22

32

Nerol

10

14

iii)

Geraniol

23

32

iv)

Phenylethyl

(1)
the relevant

Indian

Standard.

i)

ii)

(2)

7.3 Quality of Reagents

Unless specified otherwise, pure chemicals and distilled

alcohol

1S 15740:2007

ANNEX A
(Ckum 4.2)
GAS CHROMATOGRAPHIC ANALYSIS OF OIL OF ROSE

I,

A-1 GENERAL

Ramp/Temperature
programme

4.5 OC/min

The chromatographic analysis is given on capillary column.

The chromatographic conditions given here are for
information and guidance.

Final temperature

200 c

Initialtime

Smin

A-1.1 Outline of the Method

Injector port temperature:

A sample of the material is dissolved in a suitable solvent

(for example, hexane, cyclohexane or petroleum ether) and
is injected into the gas chromatographywhere it is carried
by the carrier gas from one end of the column to the other.
During its movement, the constituents of the sample
undergo distribution at different rates and ultimately get
separated from one another. The separated constituents

210 T

Detector
a) Type

: Flame ionization

b) Temperature

220C

Volumeinjected

0.2 pl

emergefromthe end of the columnone afieranotherand

are detectedby suitablemeanswhoseresponseis related

A-3 CALCULATION

to the amount of a specific component leaving the column.

A-3.1 Area Measurements (see Note 1)

A-2 PROCEDURE
A-2.1 The analysis shall be done as per IS 326 (Part 19).
The typical chromatograms for oil of rose in capillary
column with the following chromatographic conditions is
shown in Fig. 1.
A-2.1.1 Gas Chromatographic Conditions of Capillary
Column
Column

: Fused silica capillary

column

a) Size

Length, 30 m
lntemal diameter, 0.23 m

b) Carrier gas

Nitrogen

Stationery phase

Carbowax, 20 m

Since normal peaks approximate a triangle, the area is

measured by multiplying the peak height times the width
of the half height. The normal peak base is not taken since
large deviations may be observed due to tailing or
adsorption. This technique is rapid, simple and fairly
accurate when peaks are symmetrical and of reasonable
width.
A-3.2 Area Normalization (see Note 2)
By normalizing, it is meant calculating the area of each and
dividing the individual areas by total areas,
Percentage ofA =

Area of A x 100
Total area

NOTES

1 Other

methods of area measurements,

disc integrator

and electronic

digital

namely, triangulation,
integrator,

if fixed with

GC machine, would be of great advantage.

Conditions
Initial temperature

2 Internal

50c

standardization

internal standard is available.

or indirect calibration.

can be used, if pure appropriate

This method is known as relative

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Is 15740:2007

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Bureau of Indian Standards

,
.,

BIS is a statutory institution established under the Bureau of Indian Standards Act, 1986 to promote
harmonious development of the activities of standardization, marking and quality certification of goods and
attending to connected matters in the country.
Copyright
BIS has the copyright of all its publications. No part of these publications may be reproduced in any form
without the prior permission in writing of BIS. This does not preclude the free use, in the course of implementing
the standard, of necessary details, such as symbols and sizes, type or grade designations. Enquiries relating to
copyright be addressed to the Director (Publications), BIS.
Review of Indian Standards
Amendments are issued to standards as the need arises on the basis of comments. Standards are also reviewed
periodically; a standard along with amendments is reaffimed when such review indicates that no changes are
needed; if the review indicates that changes are needed, it is taken up for revision. Users of Indian Standards
should ascertain that they are in possession of the latest amendments or edition by referring to the Iated issue of
BIS Catalogue and ktandards: Monthly Additions.
This Indian Standard has been developed from

DOC:

No.

PCD 18 (1855).

Amendments Issued Since Publication

Date of Issue

Amend No.

Text Aflected

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