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Cite this article as: Chin J Anal Chem, 2014, 42(1), 2127.
RESEARCH PAPER
MicroreagentDispensingMethodBasedonPulseDriving
&ControllingofMicrofluidsTechnologyandApplication
Research
ZHENG Yue*, HOU Li-Ya, ZHU Li, WANG Hong-Cheng, HE Jia-Qiao, ZHANG Wei-Yi
School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China
Abstract: With the development of bioanalytical technique and high throughput screening techniques, the amount of reagent was
decreased to nanoliter and even picoliter level. To achieve high precision of micro-reagent dispensing, a two-channel micro-reagent
dispensing system based on pulse driving & controlling technique of micro-fluids was developed. Then, taking glycerine solution as
dispensing reagent, influences of viscosities, inner diameter of the micro-nozzle, driving frequency, and voltage amplitude on the
dispensing volume were investigated. With the voltage amplitude of 70 V, driving frequency of 4 Hz, and 100 m inner diameter of
the micro-nozzle, different ratios of Na2HPO4 to KH2PO4 solution were prepared to study the mixing-reaction. In the experiment, a 3
3 phosphate buffer arrays solution with a pH gradient was first prepared. pH indicator was then added to detect the acidity and
alkalinity of the mixed solution. The results showed that the relative standard deviation (n = 9) of spots diameter of the prepared pH
gradient arrays was 0.8%; the spots were fully reacted, and uniform colors and significantly changed gradients were achieved. By the
micro-reagent dispensing method based on pulse driving & controlling of micro-fluids technique, parallel, automated and micro
volume (picoliter) dispensing with higher dispensing precision and higher repeated accuracy could be realized, and especially, micro
reaction with different ratios could be achieved without separate reaction pools.
Key Words:
Introduction
ZHENG Yue et al. / Chinese Journal of Analytical Chemistry, 2014, 42(1): 2127
ZHENG Yue et al. / Chinese Journal of Analytical Chemistry, 2014, 42(1): 2127
ZHENG Yue et al. / Chinese Journal of Analytical Chemistry, 2014, 42(1): 2127
3.4
Dispensing volume
(pL)
RSD
(%, n = 50)
810
746
696
628
533
0
2.5
2.1
2.0
1.9
1.7
-
1
5
15
25
35
> 35
Table 2 Relation between different micro-nozzle inner diameters and dispensing volumes
Micro-nozzle inner diameter (m)
25
50
75
100
125
150
175
200
24
105
242
715
1210
2146
3343
2041
10
15
20
25
30
> 30
564
1.9
554
1.6
536
1.4
521
2.2
502
2.1
486
3.2
470
3.5
458
4.1
443
5.8
Unstable
-
ZHENG Yue et al. / Chinese Journal of Analytical Chemistry, 2014, 42(1): 2127
< 20
20
30
40
50
60
70
80
0
122
1.3
159
1.6
214
2.2
280
1.8
395
2.1
536
1.4
708
2.0
ZHENG Yue et al. / Chinese Journal of Analytical Chemistry, 2014, 42(1): 2127
Conclusions
Table 5 Spot diameters, ratios, the corresponding pH and the color change rule
No.
Spot diameter(m)
Ratio
pH
Color change rule
A1
A2
A3
B1
438
1:9
440
2:8
439
3:7
444
4:6
B2
444
5:5
Acidity
Yellow
B3
C1
C2
C3
RSD (%, n = 9)
444
6:4
448
7:3
444
8:2
Basicity
Blue
448
9:1
0.8
Green
ZHENG Yue et al. / Chinese Journal of Analytical Chemistry, 2014, 42(1): 2127
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