Фотометр для микропланшет модель 680 & 680 XR (Bio-Rad) СИ PDF

Life Science Group
Model 680 and 680 XR

Microplate Reader
Service Manual
Part Number 100-2414 Revision E
PROPRIETARY NOTICE
Information contained in this manual is copyrighted by Bio-Rad Laboratories, Inc. None of the contents may be
reproduced in full or in part by any person without the prior written approval of Bio-Rad Laboratories, Inc. This
manual is intended to provide information to assist the qualified field or instrument service technician to repair,
maintain, and order replace parts for the instrument supplied. Every reasonable effort has been made to keep
the information in this manual current and accurate as of the date of publication or revision. However, no guarantee is given or implied that the manual is error-free or accurate about any specification.
Copyright 2002-2006 by Bio-Rad Laboratories, Incorporated. All rights reserved.

Bio-Rad Laboratories Inc, Life Science Group
2000 Alfred Nobel Drive
Hercules, California 94547
Telephone: 1-800-424-6723
100-2414E 02/06
About this manual
This
document is part of Life Science Groups commitment to provide

you, the service professional, with adequate information to effectively
support Bio-Rad instruments. Additional copies of this service document
may be ordered using part number 100-2414.
We welcome and appreciate any suggestions you may have to improve

our service documentation. Please address your comments or suggestions to:
Technical Editor, Global Service Support Dept.
Bio-Rad Laboratories, Life Science Group
2000 Alfred Nobel Drive
Hercules, CA 94547
FAX Number: 510 741-5802
ii
100-2414E 02/06
Revision Control List

Document: Model 680 and 680 XR Microplate Reader Service Manual
Part Number: 100-2414
Date of Latest Revision: February 2006
Each page of this manual has the catalog number, revision level and revision date for that page. Subsequent
revisions to a page will be indicated by changes to the revision level and revision date for that page. A summary of changes for all revisions is listed in the following table.
Revision
Page
Number
4-11 to 4-12
Initial Release
A. Gandy
Feb. 2002
Updated Checkmark Plate procedure
A. Gandy
June 2002
A. Gandy
Mar 2004
A. Gandy
Sept 2005
A. Gandy
Feb 2006
Added optics cleaning procedure
6-1 to 6-25
Added part numbers to sections; Updated Figure 6-9
7-1 to 7-2
Added Checkmark Plate; Updated Figure 7-3

Added Section AdjustingPlate Carrier Belt Tension
7-6
Added Section Alignment of Plate Carrier
8-5
Added Table 8-4 Checkmark Plate Failures
Sec 1 thru 10
Manual Updated to Add Model 680XR
5-1
Added Note
6-3
Added 6.3.3 Model 680 XR Lamp
6-12
Added Table 6-1

Added 6.3.11 Model 680 XR Lamp Control PCB
6-22
Added 6.3.17 Model 680 XR Light Balancing Filter
7-8
Added Table 2 Firmware History
8-1
Updated Table 8-1
9-3 to 9-11
App D
D
Added check of plate position
7-4
6-15 to 6-16
Date
5-1 to 5-4
6-16
Changed
By
Description
Updated parts information

Added Appendix D
6-12
Added Backlit LCD to Table 6-1
7-6
Updated to reference Section 7.7
7-9
Updated Firmware Table 7-2 and added 7.6.3
7-10 to 7-11
Added Section 7.7 QC Software
9-5 to 9-11
Updated parts
7-8 to 7-9
Updated Firmware version 1.71; Table 7-2 update
7-11 to 7-15
100-2414E 02/06
Updated 7.6.4 Firmware Downloading
7-17
Added Section 7-8 External Printer Setup
8-1
Updated troubleshooting printer
9-11
Added Shield Cover
iii
Table of Contents
Section 1 Introduction
1.1 General Inforation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2 Service Philosophy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.3 Safety and Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.4 Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Section 2 System Overview

2.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2 Product Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.3 Product Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.4 Regulatory Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.5 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.5.1
Calculations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.5.2
Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.5.3
Memory Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.5.4
Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2.5.5
RS-232 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2.6 Instrument Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

2.6.1
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.6.2
Optical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.6.3
Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.6.4
Thermal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2.6.5
Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2.6.6
Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Section 3 Installation
3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Visual check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.3 Installation Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.4 Install Paper Roll (Optional Internal Printer) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.5 Computer Interface (Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Section 4 System Checkout

4.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.2 Tools and Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.3 Summary of Tests and Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.4 System Checkout Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.4.1
General. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.4.2
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.4.3
Preparing Yellow Dye Test Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Table of Contents
4.4.4
Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.4.5
Touch Pad/Mixing Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.4.6
Verify Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
4.4.7
Start Microplate Manager . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
4.4.8
Air Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.4.9
Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.4.10 Channel-to-Channel Variation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8

4.4.11 Stability and Drift . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
4.4.12 Verify Incubator Temperature (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
4.5 Checkmark Validation Program (Optional Method) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11
4.5.1
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11
4.5.2
Procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11
Section 5 Preventative Maintenance

5.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2 Tools and materials
5.3 Preventative Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.3.1
Firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.3.2
Inspection of Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.3.3
Perform System Checkout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Section 6 Removal and Replacement

6.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.3 Removal and Replacement Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.3.1
Interference Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.3.2
Model 680 Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.3.3
Model 680 XR Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
6.3.4
Top Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4
6.3.5
Printer Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5
6.3.6
Keypad Membrane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-7
6.3.7
Inlet Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-8
6.3.8
Plate Carrier Assembly and Analog Board (#282) . . . . . . . . . . . . . . . . . . . . . . . . 6-9
6.3.9
CPU Board (#281) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-12
6.3.10 Incubator Control Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-13

6.3.11 Model 680 XR Lamp Control PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-15
6.3.12 Power Supply, 12 Volt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-17
6.3.13 Power Supply, 24 Volt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-18
6.3.14 Photo Sensor (Analog Board) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-19
Table of Contents
6.3.15 Photo Sensor (Filter Wheel) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-20

6.3.16 Model 680 Light Balancing Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-21
6.3.17 Model 680 XR Light Balancing Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-22
6.3.18 Plate Carrier Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-23
6.3.19 Optical Fiber Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-25
6.3.20 Filter Wheel/Filter Wheel Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-27
6.3.21 Fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-28
6.3.22 Lithium Battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-29
Section 7 Adjustment and Calibration

7.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.3 Electronics Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.3.1
Setting of Jumper on the CPU Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.3.2
Setting of Jumpers on the Printer Control Board . . . . . . . . . . . . . . . . . . . . . . . . . 7-2
7.3.3
Incubator Electronics Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3
7.4 Adjusting Plate Carrier Belt Tension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4

7.5 Plate Carrier Alignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6
7.5.1
Checking of Plate Alignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6
7.5.2 Adjusting Plate Alignment - Y Direction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-7

7.5.3 Adjusting Plate Alignment - X Direction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-7
7.6 Firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-8
7.6.1
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-8
7.6.2
Firmware History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-9
7.6.3
Integration of English and Chinese Firmware Version . . . . . . . . . . . . . . . . . . . . . 7-10
7.6.4
Downloading Firmware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-11
7.7 QC Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16

7.7.1
Entering QC Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16
7.7.2
Monitor Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16
7.7.3
Plate Positioning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-17
7.8 External Printer Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-17
Section 8 Troubleshooting
8.1 About this Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
8.2 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
Table 8-1 General Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
Table 8-2 Error Codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-2
Table 8-3 Firmware Downloading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4
Table of Contents
Section 9 Parts Information

9.1 About this Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
9.2 Parts Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
9.2.1
US Domestic Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
9.2.2
International Subsidiaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
9.3 Returning Defective Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

9.3.1
US Domestic Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
9.3.2
International Subsidiaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
9.4 Service Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-3

Table 9-1 Replaceable Parts for the Model 680 Microplate Reader . . . . . . . . . . . . . . . . 9-3
Table 9-2 Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-11
9.5 Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-12
Table 9-3 Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-12
9.6 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-13
Table 9-4 Special Service Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-13
9.7 Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-14
Table 9-5 Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-14
Section 10 Drawings
10.1 About this Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1
10.2 Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1
Block Diagram
Wiring Diagram
Appendix
Appendix A
Appendix B
Appendix C
Appendix D
Model 680 System Checkout Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1

Model 680 XR System Checkout Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
Checkmark Check Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
Error Flow Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-1
Introduction
Section 1
Introduction
1.1
General Information
This manual is the reference to be used for troubleshooting, repair, and maintenance of the Model 680 and
Model 680 XR Microplate Readers. It is intended to be used by trained technicians and engineers familiar with
safety precautions relative to working with instrumentation. The procedures in this manual assume that the
reader is familiar with the operation of the Model 680 and 680 XR Microplate Readers. Complete operating
instructions are contained in the Model 680 and 680 XR Microplate Reader instruction manual. Information
presented in the instruction manual is not repeated in this Service Manual unless there is a compelling reason
to do so.
1.2
Service Philosophy
This manual is designed to support mail in products. Regional service centers are the first level of service,
where faulty assemblies can be most effectively repaired at a central repair facility. The second level of service
is performed at our factory instrument service facility in Richmond, California, where additional service procedures requiring resources not available at regional service centers are performed.
1.3
Safety and Precautions
The Model 680 and 680 XR Microplate Reader contains the following Hazards:
Hazardous voltages
Thermally hot components
The following should be observed when servicing this unit:
When power is required, the unit should be plugged into an outlet with a properly grounded receptacle.
The use of 2-prong plug adapters is not recommended and will void the safety certification of this
product.
The use of extension cords may compromise the safety of the operator and is not recommended.
Ensure that fuses installed in the unit are of the correct rating.
Ensure that none of the ventilation openings in the unit are blocked. Excessive heat buildup in the unit
may cause failures.
Bio-Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other
than for which it is intended or by modifications of the instrument not performed by Bio-Rad or an authorized
agent.
1.4
Conventions
The following conventions are used throughout this manual.:

Left and right sides of the unit are as viewed from the front, operators position, unless otherwise stated.
Dimensions are in millimeters, unless otherwise stated.
100-2414C 03/04
1-1
System Overview
Section 2
System Overview
2.1
Introduction
The Model 680 and Model 680 XR Microplate Readers are an eight-channel, vertical pathlength photometer
that measures absorbance of the contents of 96 well mictotitration plates. They can perform single or dual
wavelength measurements, and can report absorbance values to three decimal places.
The Model 680 and 680 XR Microplate Readers can be programed by entering commands through the keypad
to define plate reading conditions and data analysis conditions as a protocol data, and select report types. A
printed report is produced by an optional on-board thermal printer or external printer. An optional incubator is
also available.
The Model 680 and 680 XR Microplate Readers feature a built-in RS-232C serial interface device for convenient computer interfacing. The Microplate Manager software for personal computers offers a complete analysis program. Microplate Manager supports Windows 95, Windows NT and Windows 2000.
Microplate Manager Version 5.2.1 added support for the Model 680XR reader.
2.2
Product Description
Figure 1: Model 680/680 XR Microplate Reader

The following items are included with the Model 680/680 XR Microplate Reader system:
Microplate Reader with 415 nm, 450 nm, 490 nm and 655 nm interference filters installed
Power Cord
Spare Fuse
Dust Cover
Instruction Manual
Warranty Card
100-2414C 03/04
2-1
System Overview
2.3
Product Configurations
The Model 680 Microplate Reader is available in four configurations.
Table 1: Product Configurations

Catalog Number
2.4
Description
168-1000
Model 680 Microplate Reader

No Temperature Control and No Internal Printer
168-1001

With Temperature Control and No Internal Printer
168-1002

No Temperature Control and With Internal Printer
168-1003

With Temperature Control and With Internal Printer
168-1100
Model 680 XR Microplate Reader

No Temperature Control and No Internal Printer
168-1101

With Temperature Control and With Internal Printer
Regulatory Notices
This instrument is designed to meet Class A emissions and immunity levels of EN61326 product family standard for CE mark. This rating requires that it only be used in a laboratory environment.
This instrument is designed and certified to meet EN61010 requirements which are internationally accepted
electrical safety standards. Certified products are safe to use when operated in accordance with the Instruction
Manual. This instrument should not be modified or altered in any way. Alteration of this instrument will result in
voiding of the manufacturers warranty, void regulatory certification and create a potential safety hazard.
This equipment has been tested and found to comply with the limits for Class A digital device, pursuant to Part
15 of FCC rules. These limits are designed to provide a reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This equipment generates, and can radiate
radio frequency energy and if not installed and used in accordance with the Instruction Manual, may cause
harmful interference to radio communications. Operation of equipment in a residential area is likely to cause
harmful interference in which case the user will be required to correct the interference at their expense.
2-2
100-2414C 03/04
System Overview
2.5
Product Overview
2.5.1
Calculations
The Model 680 and 680 XR Microplate Readers uses Beers Law to calculate the absorbance value of each
well. Beers Law states that absorbance is equal to the Log10 of the ratio on the baseline measurement
intensity (Io) to the Sample measurement intensity (I).
Beers Law:
Absorbance = Log10 (Io/I)
Before measuring the plate, the reader takes a reading for all eight photodiode channels. These values are
recorded as the baseline measurement (Io) values for each channel, respectively. The reader then records
the sample measurement (I) value for each well, and calculates the absorbance using these values. Channel-to-channel error is significantly reduced because the Io value for a given channel is used only in determining the absorbance of the wells of that channel.
2.5.2
Limits
The Model 680 and 680 XR Microplate Readers display absorbance readings with absolute values up to
3.500. Absolute values greater than 3.500 are out of range and are displayed as either *.*** or -*.***.
2.5.3
Memory Backup
The battery backup provides memory even after the reader is turned off. The following information will be
saved in memory until new reading parameters are set by the user or a new plate is read by the instrument.
Note: If reading a new plate is aborted before it is finished, all the previous plate reading date will remain in
memory and none of the new data from the new plate is retained.
1.
10 latest plate reading data for End point protocol
2.
2 latest sets of Kinetic plate reading data (maximum 30 series of readings for each Kinetic)
3.
End Point and kinetic protocol data
4.
A set of Checkmark protocol data and the latest set of Checkmark read data
5.
8 wavelength values for filters
6.
Laboratory name
7.
2 security passwords (Administrator and Common user)
The first time the instrument is turned on, or after a battery replacement, the following default information will
hold in memory:
1.
Both Administrator and Common user security password are set to 00000
2.
Laboratory name is Bio-Rad Laboratories
3.
All wavelength values of the filters are set to --- nm
4.
The date and time of the calendar chip are set to 1 day, 1 month, 00 year, 0 hour and 0 minutes
5.
None of the plate reading data is available
6.
End Point protocol is chosen and the protocol number is set to #1
100-2414C 03/04
2-3
System Overview
2.5.4
Reports
Eight types of reports for End Point protocol can be generated by the Model 680 Microplate Reader:
1.
2.
3.
4.
5.
6.
7.
8.
2.5.5
Raw Data Report

Absorbance report
Limit Report
Matrix Report
Cut Off report
Curve Fit Report
Concentration Report
Difference report
RS-232 Interface
The Model 680 Microplate Reader has a built-in RS232C interface (9 pin D-sub connector). This allows
external computers to control the instrument. When an external computer is in control of the microplate
reader, the printer and the keypad keys (except Start/Stop) are automatically deactivated and the display
will indicate Remote Mode.
The reader will remain in the remote control mode until it receives RL command from the host computer or
until the Start/Stop key is pressed.
RS-232 communication parameters are as follows:
Signals: TDX: Transmit Data
RXD: Receive Data
DTR: Data Terminal Ready
DSR: Data Set Ready
CTS: Clear to Send
RTS: Ready to Send
Transmission: Baud Rate 9600, Data Width 8, Stop Width 1, Parity None
Data: ASCII (no distinction between upper and lower case)
Signal Assignment:
Host Computer
9 Pin D-sub
2-4
Model 680 Reader

9 Pin D-sub
Receive Data 2
------------------------
3 Transmit Data
Transmit Data 3
------------------------
2 Receive Data
Data Terminal Ready 4
------------------------
6 Data Set Ready
Signal Ground 5
------------------------
5 Signal Ground
Data Set Ready 6
------------------------
4 Data Terminal Ready
Request to Send 7
------------------------
8 Clear to Send
Clear to Send 8
------------------------
7 Request to Send
100-2414C 03/04
System Overview
2.6
Instrument Specifications
The Model 680 and 680 XR Microplate Reader specifications are as follows:
2.6.1
Features
Operating Panel: Membrane pad with 4 arrow keys, 7 function keys and 11 numeral keys
Display: LCD, 4 Lines x 20 Alphabet Characters
Computer Interface: Bi-directional RS-232C serial communication port, 9 pin D-sub receptacle
External Printer Port (only when internal printer option is not installed): Centronics interface with ASCII
character code; 25 pin D-sub receptacle; 80 characters output per line
Report Types: Raw, absorbance, matrix, limit, cutoff, concentration, curve fit, difference, kinetic
Applicable Plates: Standard 96 well microtitre plate of polystyrene - flat, U, V rigid bottom
Mixing: 3 speeds (low, medium, high); Programmable time period 0-999 seconds
Warm-up Time: 3 minutes
2.6.2
Optical
Photometric Methods: Single and dual wavelength

Photo detectors: 8 silicon diodes for measurement and 1 silicon photodiode for reference
Light Source: Tungsten halogen lamp, 20 Watts with 3000 hours average lifetime
Model 680 Spectral Range: 400 nm to 750 nm
Model 680 XR Spectral Range: 340 nm to 800 nm
Interference Filters: Wavelength Tolerance +/- 3 nm; Band Width: 10 nm typical
Filter Capacity: 8 filters
Model 680 Standard Filters: 415, 450, 490, 655 nm
Model 680 XR Standard Filters: 340, 405, 415, 655 nm
Optional Filters: 340 through 800 nm (in 5 nm increments)
Fast Reading Speed: 6 seconds with single wavelength reading; 10 seconds with dual wavelength
reading
Standard Reading Speed: 15 seconds with single wavelength reading; 30 seconds with dual wavelength reading
Indication Range: 0.000 to 3.5 OD
2.6.3
Performance
Resolution: 0.001 OD
Accuracy: +/- 1.0% (or 0.010 from 0 to 3.0 OD at 490 nm)
Linearity: +/- 1.0% (from 0 to 2.0 OD); +/- 2.0% (from 2.0 to 3.0 OD)
Reproducibility: 1.0% (or 0.005 from 0 to 2.0 OD); 1.5% (from 2.0 to 3.0 OD)
Inter-Channel Variation: 1.5% (or 0.005 from 0 to 3.0 OD)
Stability and Drift: 0.010 OD at OD=1 with single wavelength reading
100-2414C 03/04
2-5
System Overview
2.6.4
Thermal
Incubator (Optional): Temperature Set Point: 25oC to 45oC (at least 5oC above ambient)
Set Point Accuracy: +/- 0.5oC (set point = 37oC and room temperature = 25oC)
Well-to-Well Uniformity: 0.5oC (set point = 37oC and room temperature = 25oC)
Warm-up Time: 5 minutes (set point = 37oC and room temperature = 25oC)
2.6.5
Electrical
Line Voltage: Universal Input: 100-120 VAC, 200-240 VAC, 50/60 Hz

Fuse: T3.15 Amp, 250 Volts, 20mm x 5mm
Power Consumption: 100 VA maximum
Battery: Lithium, CR2032, 3 Volts; average lifetime 5 years
2.6.6
Physical
Dimensions: 340 x 330 x 152 mm

Weight: 5.5 kg
Operating Environment: 5oC to 35oC, 0 to 95% RH, non condensing
Storage Environment: -20oC to 50oC, 0 to 95% RH, non condensing
2-6
100-2414C 03/04
Installation
Section 3
Installation
3.1
Introduction
The Model 680 and 680 XR Microplate Readers are easy to install. Prior to installation, verify that all system
components have been received.
3.2
Visual Check
1.
Check this instrument for any signs of damage which may have occurred during shipping.
2.
Check the filters: Open the rear cover by pushing the release latch on the top. Check that the filters
are installed into the proper positions per Table 1:
Table 1 - Filter Installation
3.
Filter Position
Model 680
Model 680XR
415 nm
340 nm
450 nm
405 nm
490 nm
415 nm
655 nm
655 nm
User
User
User
User
User
User
User
User
Check to insure filters are properly seated (Figure 1).
Figure 1: Filter Check
100-2414C 03/04
3-1
Installation
3.3
Installation Procedure
1.
Place the instrument on a clean, sturdy table or bench. It is important to keep the instrument in a
clean, relatively dust free environment to ensure maximum performance.
2.
Connect the power cord to the connection on the back if the instrument.
3.
Turn ON the power switch on the rear panel. The LCD will display the version number of the on board
firmware. After about three seconds, the instrument will perform a self test diagnosis that takes about
fifteen seconds.
4.
When prompted by the Log In screen, enter the password (password = 00000). Press Enter.
5.
Allow three minutes for the instrument to warm up prior to reading plates.
3.4
Install Paper Roll (Optional Internal Printer)

1.
Open the rear cover by pushing the release latch on the top.
2.
Tear off a small piece of paper to form a diagonal point on the end of the paper roll.
3.
Place the paper roll in the holder positioned below the printer and orient the roll so that it will feed
from the bottom (Figure 2).
Figure 2: Orientation of Paper Roll

4.
3-2
While pressing the Paper Feed key, direct the pointed end of the paper into the paper feed slot in the
underside of the printer until the printer grabs the paper and feeds it through the slot in the top of the
instrument.
100-2414C 03/04
Installation
3.5
Computer Interface (Optional)

1.
Some customers may utilize the system with a computer and microplate software such as Microplate
Manager (or Checkmark).
2.
Set up computer as required and install software.
3.
Install serial cable from computer to Model 680 oe 680 XR.
4.
Turn on instrument, enter password.
5.
Verify communication with instrument using Microplate Manager.
Note: Microplate Manager version 5.2.1 is required for Model 680XR support.
100-2414C 03/04
3-3
System Checkout
Section 4
System Checkout
4.1
Introduction
This section provides the system checkout procedure for the Model 680 and 680 XR Microplate Reader. Successful completion of the tests in this section represent the Operational Qualification of the instrument. A System Checkout Report is available in Appendix A and B.
The system checkout procedures for the Model 680 and 680 XR Microplate Readers require the use of a
remote computer using Microplate Manager software.
The Model 680 and 680 XR Microplate Reader contains an on board checkout procedure, Checkmark Validation Program, which requires the use of a special Checkmark test plate.
4.2
Tools and Materials
Microplate Manager Software, 170-9520

RS-232 Cable, 931-0184
Printer
Digital Temperature Multi-meter with 0.1 mm diameter bead type K thermoprobe (minimum +/- 0.5 C
temperature meter accuracy)
96 Well Microtitre Plate, Flat Bottom, 224-0096
Micro Pipette, 50L
Pipette Tips
50 ml beaker
Acid Yellow 99, Fisher AC19178-100
Distilled Water
Tween 20, 170-6531
Checkmark Reader Performance Check Plate, 170-6940 (Optional)
100-2414C 03/04
4-1
System Checkout
4.3
Summary of Tests and Specifications
The following is a summary of the tests and specifications for the Model 680 and 680 XR Microplate Readers.
Table 1: Summary of Tests and Specifications
Test
Specification
Firmware
Firmware Version
Record Version
Instrument Function
Keypad and Display
Proper Function
Internal Printer (optional feature)
Proper Function
Startup and Self Diagnosis
Proper Function
RS-232 Communication
Proper Communication
Mixing Feature
Proper Function
Incubator Set Point 37 C (optional feature)
37 C +/- 0.5 C
Air Read
< 0.005 OD
Accuracy
CV < 5%
Channel-to-Channel
CV < 5%
Stability and Drift
< 0.010 OD
System Checkout
4-2
Parameter
100-2414C 03/04
System Checkout
4.4
System Checkout Procedure
4.4.1
General
1.
All system checkout procedures in this section will be performed with Microplate Manager via PC
computer.
2.
The Checkmark Validation Program, if available, may be used for system checkout as an alternate
method (paragraph 4.5).
4.4.2
Setup
1.
Setup computer.
2.
Install Microplate Manager.
3.
Install HPK protection key.
4.
Connect RS-232 cable from computer to reader.
4.4.3
Preparing Yellow Dye Test Plate
1.
System checkout requires the preparation of a yellow dye pipetted into a microtitre plate. Pipetting
must be of extreme accuracy as the Model 680 reader is sensitive enough to detect pipetting errors.
2.
Prepare the yellow dye as follows:

Mix: 2 mg Acid Yellow 99
50 ml Distilled Water
0.01 ml Tween 20
3.
Pipette 200 microliters yellow dye into each well of the microtitre plate (96 total).
4.
The value of the prepared test plate is 0.424 - 0.444 OD.
4.4.4
Startup
1.
Turn ON the reader power switch. The LCD will display the version number of the on board firmware.
Record Firmware Version.
2.
After about three seconds, the instrument will perform a self test diagnosis that takes about ten seconds.
3.
When prompted by the Log In screen (Administrator), enter the password 00000. Press Enter.
4.4.5
Touch Pad/Mixing Function
1.
Open chamber door (leave open for this test).
2.
Press Memory Recall button.
3.
Select Protocol and press Enter.
4.
Select End Point and press Enter.
5.
Select End Point 01 and press Enter twice.
6.
Press the Start button.
7.
Observe that plate carrier vibrates for 3 seconds prior to read.
100-2414C 03/04
4-3
System Checkout
4.4.6
Verify Printer (Optional Feature)
1.
After completion of the above End Point 01 test, press the Print button.
2.
Select Reports and press Enter.
3.
Verify the printer is functioning properly.
4.4.7
Start Microplate Manager
1.
Start Microplate Manager.
2.
Select File...Select Reader. Select Model 680 or Model 680 XR and click OK.
3.
Select New Endpoint Protocol. The Microplate Manager window will appear (Figure 1).
Figure 1: Microplate Manager - Endpoint Protocol

4.
4-4
The instrument will be placed in remote mode. Remote Mode will appear on display and touch keys
will become nonfunctional.
100-2414C 03/04
System Checkout
4.4.8
Air Read
1.
This test is performed without the use of a microtiter plate.
2.
Press Show Template button.
3.
Click the Turn Off Autofill Icon.
4.
Click the Format as Unknowns Icon.
5.
Using the curser, click and drag highlighting all rows (A1 to G12).
6.
All wells on the template should be squares filled with 1s (see Figure 2).
Figure 2: Plate Template

7.
Minimize the plate template.
8.
Click on Reports.
9.
Check the Raw Data Report. Click OK. (See Figure 3).
Figure 3: Raw Data Report Setup
100-2414C 03/04
4-5
System Checkout
10. Check the Reading Parameters Single box.

11. Select Measurement filter: 490.
12. Click the Run button.
13. Plate will take a few moments to read and display the Raw Data Report.
14. Ensure that all values are < 0.005. Reference Figure 4.
Figure 4: Raw Data Report
4-6
100-2414C 03/04
System Checkout
4.4.9
Accuracy
1.
This test is performed with the use of the yellow dye test plate.
2.
Use the formatted plate template from the Air Read above.
3.
Click on Reports.
4.
Check the Raw Data Report and Unknown Concentrations. Click OK. See Figure 5.
Figure 5: Report Selection

5.
Check the Reading Parameters Single box.
6.
Select Measurement filter: 490.
7.
For Mix Time (sec) enter 3.
8.
Click the Run button.
9.
Ensure the CV is < 5% on the Unknown Concentrations Report (Figure 6) and target values are
within expected ranges (Mean OD).
Figure 6: Unknown Concentrations Results
100-2414C 03/04
4-7
System Checkout
4.4.10 Channel-to-Channel Variation

1.
This test is performed with the use of the yellow dye test plate.
2.
Press Show Template button.
3.
Click the Turn Off Autofill Icon.
4.
Click the Format as Unknowns Icon.
5.
Using the curser, click and drag highlighting rows A1 to A12. The number 1 should appear in each
box. Repeat highlighting rows B1 to B12. The number 2 should appear in each box. Repeat for the
remaining rows C1 to C12, D1 to D 12, etc. All wells on the template should appear as shown in Figure 7.
Figure 7: Channel to Channel Setup

6.
Minimize the plate template.
7.
Use the same format for reports (Raw Data Report and Unknown Concentrations), filter (490)
and mix time (3).
8.
Click the Run button.
9.
Plate will take a few moments to read and display the Raw Data Report and Unknown Concentrations Report.
10. Ensure the CVs are < 5% on the Unknown Concentrations Report (Figure 8) and target values are
within expected ranges (Mean OD).
4-8
100-2414C 03/04
System Checkout
Figure 8: Unknown Concentrations Report
4.4.11 Stability and Drift

1.
This test uses the same setup as specified in 4.4.11 Channel-to-Channel Variation above.
2.
Rerun the Channel-to-Channel variation test 3 times allowing 10 minutes between tests.
3.
Compare the data from the three tests. The average mean from each row shall be within 0.010 OD of
each other.
100-2414C 03/04
4-9
System Checkout
4.4.12 Verify Incubator Temperature (Optional Feature)

1.
Place checkmark in Incubator On box. Insure set point is 37 C.
2.
Place a standard 96 well microtitre plate filled with 200 microliters water in wells A1, A6, A12, D6, D9,
H1, H6, and H12 in the plate carrier. See Figure 9.
Figure 9: Designated Temperature Test Points

3.
Allow microtiter plate to warm for 30 minutes.
4.
After 30 minutes, immerse the thermoprobe in the designated well filled with the 200 microliters of
water without touching any portion of the inner well wall. The immersed probe must be in the middle
of the well and immersed in water. See Figure 10.
Figure 10: Well Temperature Measurement

5.
4-10
Verify that the actual well temperature is 37 C +/- 0.5 C for each well (A1, A6, A12, D6, D9, H1,
H6, and H12).
100-2414C 03/04
System Checkout
4.5
Checkmark Validation Program (Optional Method)
4.5.1
General
Figure 11: Checkmark Check Plate

1.
The Checkmark program is used with the Checkmark Reader Performance Check Plate for validating the performance of the Model 680 Microplate Reader. It will test the following parameters:
Default Measurements
Accuracy
Precision
Linearity
Optional Measurements
2.
4.5.2
Plate Positioning
Cross-talk
Filter Check
0 OD and Overflow
IR Spectral Blocking
The Checkmark program resides in the Model 680 Microplate Reader programs.
Procedure
Entering Checkmark Plate Reference Data

1.
Turn on Model 680 Reader and enter password.
2.
3.
4.
Select Checkmark and press Enter. Press any key if instructed.
5.
Press the Edit button.
6.
100-2414C 03/04
4-11
System Checkout
7.
Select Serial/Report and press Enter
8.
Enter serial number of Checkmark plate. Select report type Simple or Full. Press Enter.
9.
Select Linearity and press Enter.
10. Enter appropriate data from Checkmark reference plate data. Press enter when complete.
11. Select Max CV and press Enter.
13. Select Filter Check Reference and press Enter.
Running the Test
4-12
1.
Open chamber door, insert Checkmark Reader Performance Check Plate, and close door.
2.
3.
4.
Select Checkmark and press Enter. Press any key if instructed.
5.
Press the Start button.
6.
Test will take about 3 minutes to read plate.
100-2414C 03/04
Preventative Maintenance
Section 5
5.1
Introduction
This section provides the preventative maintenance procedure for the Model 680 and 680 XR Microplate
Reader. Preventative maintenance is recommended at least once a year.
5.2
Tools and Materials
Small Hand Tools

Cleaning Materials (Lint Free Cloth, Lens Tissue, Isopropyl Alcohol)
5.3
5.3.1
Firmware
1.
Connect the power cord to the connection on the back if the instrument.
2.
Turn ON the power switch on the rear panel. The LCD will display the version number of the firmware.
3.
Record firmware version.
4.
Turn OFF instrument.
5.
Update to latest firmware if applicable (see Section 7.6).
5.3.2
Inspection of Instrument
1.
Remove top cover.
2.
Visually inspect for cleanliness of plate carrier. Clean plate carrier and rails with lint free cloth and
isopropyl alcohol.
Note: Do not use grease on the plate carrier or rails.

3.
Visually inspect for cleanliness of lenses. Clean as required with lens tissue and isopropyl alcohol
(see Section 5.3.3).
4.
Reassemble instrument.
100-2414C 03/04
5-1
5.3.3
Optics Cleaning
1.
Remove top cover.
2.
To access top lenses, remove the two screws securing the fiber optics to the cover (Figure 5-1).
Figure 5-1: Remove Screws Securing Fiber Optics

3.
Remove two hex standoffs securing top lens block.
4.
To remove lenses, remove ten securing screws (Figure 5-2).
Figure 5-2: Top Lens Block

5.
Clean lenses with lint free cloth and isopropyl alcohol.
6.
Reassemble lenses into holder and remount.
Note: Check for proper orientation when remounting.
5-2
100-2414C 03/04
7.
To access bottom lenses, remove cover (Figure 5-3).
Figure 5-3: Bottom Lenses

8.
Clean lenses as required. If removal of bottom lenses is required for cleaning, remove plate carrier
and analog board (see Section 6.3.12).
9.
Remove bottom lens block (Figure 5-4).
Figure 5-4: Bottom Lens Block Screws
100-2414C 03/04
5-3
10. Remove lenses from holder to clean (Figure 5-5).
Figure 5-5: Bottom Lens Holder

11. Clean with lint free cloth and isopropyl alcohol.
12. Reassemble instrument. Note proper orientation of bottom lens holder upon resaaembly.
5.3.4
1.
5-4
Perform System Checkout

Perform system checkout per Section 4.0 of this Service Manual.
100-2414C 03/04
Removal and Replacement
Section 6
6.1
Introduction
This section provides the parts removal and replacement procedures for the Model 680 and 680 XR Microplate
Reader.
6.2
Tools and Materials
Standard Hand Tools

Voltmeter
6.3
Removal and Replacement Procedures
6.3.1
Interference Filters
1.
2.
Rotate the filter wheel until the desired filter is in top position. Grasp top of filter frame to and pull up
to remove filter.
3.
To install filter, insert into desired slot until filter locks into place.
6.3.2
1.
Model 680 Lamp (1681006)

Caution: Surface of Cover may be Hot!
2.
Remove the two screws that hold the black plastic cover in place (Figure 6-1) and remove cover.
Figure 6-1: Screws Securing Cover
100-2414D 09/05
6-1
3.
Remove the screw which holds the metal cover in place (Figure 6-2) and remove cover.
Figure 6-2: Screw Securing Metal Cover (Caution: Hot)

4.
Carefully pull the lamp free and remove (Figure 6-3).
Figure 6-3: Lamp Removal

5.
6-2
Install new lamp in reverse order of the above steps.
100-2414D 09/05
6.3.3
1.
Model 680 XR Lamp (1681104)

Note: Surface of Cover may be Hot! Allow to cool before replacing lamp.
2.
Remove the two screws (arrows) that hold the black plastic cover in place (Figure 6-4) and remove
cover assembly.
Figure 6-4: Screws Securing Cover

3.
Remove the lamp (Figure 6-5).
Figure 6-5: Lamp Removal

4.
Install new lamp.
Note: Do not tough new lamp with fingers. Oil on lamp may reduce lamp life.
5.
Reassemble in reverse order of the above steps.
100-2414D 09/05
6-3
6.3.4
1.
Top Cover (9310161)

Remove the six screws from the underside of the instrument (Figure 6-6).
Figure 6-6: Remove Six Screws from Underside of Instrument

2.
Remove the top cover to left side of the instrument (Figure 6-7).
Figure 6-7: Remove Top Cover to Left Side of Instrument

3.
6-4
Install cover in reverse order of the above steps.
100-2414D 09/05
6.3.5
Printer Assembly (9310160)
1.
Remove top cover.
2.
Cut the cable tie securing the cable and remove cable from the circuit board (Figure 6-8).
Figure 6-8: Cut Cable Tie and Remove Cable

3.
Remove three screws securing printer assembly and remove assembly (Figure 6-9).
Figure 6-9: Three Screws Securing Printer Assembly

4.
Prior to installing printer assembly, set the jumpers on the printer control board as follows:
100-2414D 09/05
6-5
5.
Check printer head type on flexible cable (Figure 6-10).
Figure 6-10: Location of Head Type

6.
Configure the JP4 jumpers in accordance with the Figure 6-11.
Figure 6-11: JP4 Jumper Settings

7.
6-6
Install new printer in reverse order of the above steps.
100-2414D 09/05
6.3.6
Keypad Membrane (9310177, 9310277, 9310229)
1.
Remove top cover.
2.
Cut cable tie and disconnect three cables from keypad board (Figure 6-12).
Figure 6-12: Keypad Board

3.
Remove three screws and lift out keypad board (Figure 6-13).
Figure 6-13: Screws Securing Keypad Board

4.
Peel off keypad membrane (Figure 6-14).
Figure 6-14: Peel Off Keypad Membrane

5.
Install new keypad membrane in reverse order of the above steps.
100-2414D 09/05
6-7
6.3.7
Inlet Assembly (9310167)
1.
Remove top cover.
2.
Disconnect the terminals from board (Figure 6-15).
Figure 6-15: Disconnect Terminals

3.
Remove nuts from interface connectors and remove (Figure 6-16).
Figure 6-16: Remove Nuts from Connectors

4.
Remove four screws securing the fan (Figure 6-17).
Figure 6-17: Fan Mounting Screws
6-8
5.
Remove inlet assembly.
6.
Install new inlet assembly in reverse order of the above steps.
100-2414D 09/05
6.3.8
Plate Carrier Assembly and Analog Board
Note: Check the model and serial number for the correct replacement board.
Table 6-1: Analog Board Part Numbers
Analog Board
Model 680
Model 680 XR
9310182
9310289
1.
Remove top cover.
2.
Loosen the two screws which secure the fiber and pull out fiber (Figure 6-18).
Figure 6-18: Fiber Mounting

3.
Remove the nine mounting screws (Figure 6-19).
Figure 6-19: Plate Carrier Mounting Screws
100-2414D 09/05
6-9
4.
Lift the plate carrier assembly, disconnect the cables and remove assembly (Figure 6-20).
Figure 6-20: Disconnect Cables

5.
Remove two screws securing the cover over the analog board (Figure 6-21).
Figure 6-21: Analog Board Mounted to Plate Carrier
6-10
100-2414D 09/05
6.
Carefully remove fiber from mounting clips (Figure 6-22).
Figure 6-22: Clips Securing Fiber

7.
Remove four screws securing analog board and remove (Figure 6-23).
Figure 6-23: Analog Board Mounting Screws

8.
Install new plate carrier assembly or analog board in reverse order of the above steps.
100-2414D 09/05
6-11
6.3.9
CPU Board
Note: Check the model and serial number for the correct replacement board.
Table 6-1: CPU Board Part Numbers
Serial Number
CPU Board
Model 680
Old LCD
Model 680
New LCD
Model 680
Backlit LCD
Model 680 XR
10001 to 10735
10736 to 13850
13851 and higher
All
9310181
9310225
9310353
9310278
1.
Open top cover.
2.
Remove plate carrier assembly (see 6.3.8).
3.
Remove all cables to CPU board.
4.
Remove four mounting screws and remove board (Figure 6-24).
Figure 6-24: CPU Board Mounting

5.
Install new CPU board in reverse order of the above steps.
6.
Set the jumper on the CPU Board (#281) per the following table:
Table 6-2: JP2 Jumper Settings for CPU Board
Instrument
Configuration
6-12
Jumper
Jumper Placement
Without Incubator
JP2
Jump
With Incubator
JP2
No Jumper (Open)
100-2414D 09/05
6.3.10 Incubator Control Board (9310180)

1.
Open top cover.
2.
3.
Remove all cables to incubator control board.
4.
Remove four mounting screws and remove board (Figure 6-25).
Figure 6-25: Incubator Control Board Mounting

5.
Install new incubator control board in reverse order of the above steps.
6.
Perform incubator electronics adjustment as follows. Disconnect cable from plate carrier to CN52.
7.
Connect cable into Incubation Adjustment Tool, 9310183, 107.79 ohm connector on tool.
8.
Turn on instrument.
9.
Measure voltage between TP5 and TP0 (see Figure 6-26).
100-2414D 09/05
6-13
Figure 6-26: Incubator Board Adjustments

10. Adjust trim pot VR1 until the voltage measures 0.000V +/- 0.001V.
11. Connect cable into Incubation Adjustment Tool, 116.47 ohm connector on tool.
12. Measure voltage between TP5 and TP0.
13. Adjust trim pot VR1 until the voltage measures 5.528V +/- 0.006V.
14. If voltage is out of range, repeat steps 3 through 9.
6-14
100-2414D 09/05
6.3.11 Model 680 XR Lamp Control PCB (9310276)

1.
Open top cover.
2.
Remove ground screw connection and four mounting screws and lift up the filter wheel/lamp assembly (Figure 6-27).
Two Screws Backside (Not Shown)
Ground Screw
Figure 6-27: Lamp Control PCB is Located Under Lamp Assy

3.
For ease of disassembly, turn over assembly. To access lamp control board, remove the five screws
securing the lamp PCB cover (Figure 6-28).
Figure 6-28: Bottom View - Lamp PCB Cover Screws

4.
Remove cover.
Note: Heat sink will be removed as a result of removing the two side screws.
100-2414D 09/05
6-15
5.
Remove the two remaining screws securing the PCB (Figure 6-29).
Figure 6-29: Lamp Control PCB

6.
Remove three cables to lamp control PCB and lift out.
7.
Install new lamp control PCB by securing with two mounting screws and attaching the three cables.
8.
Install the cover and heat sink. For ease of assembly, position the heat sink in place prior to placing
over lamp control PCB (Figure 6-30).
Figure 6-30: Position Heat Sink

9.
Install the two screws heat sink side first, then the two remaining screws securing the cover.
10. Reassemble in reverse order of the above steps.
6-16
100-2414D 09/05
6.3.12 Power Supply, 12 Volt (9310178)

1.
Open top cover.
2.
3.
Remove four screws securing lamp housing and remove (Figure 6-31).
Figure 6-31: Lamp Housing Mounting

4.
Remove all cables to 12 volt power supply.
5.
Remove four mounting screws and remove power supply (Figure 6-32).
Figure 6-32: 12 Volt Power Supply Mounting

6.
Install new 12 volt power supply in reverse order of the above steps.
100-2414D 09/05
6-17
6.3.13 Power Supply, 24 Volt (9310179)

1.
Open top cover.
2.
3.
Remove four screws securing lamp housing and remove (Figure 6-33).
4.
Remove all cables to 24 volt power supply.
5.
Remove four mounting screws and remove power supply (Figure 6-33).
Figure 6-33: 24 Volt Power Supply Mounting

6.
6-18
Install new 24 volt power supply in reverse order of the above steps.
100-2414D 09/05
6.3.14 Photo Sensor - Plate Carrier/Analog Board (9310174)

1.
Remove top cover.
2.
Open the clip securing the optical fiber assembly. Remove the three screws securing the cover and
remove (Figure 6-34).
Figure 6-34: Remove Clip and Mounting Screws

3.
Remove the screw securing the photo sensor and remove (Figure 6-35).
Figure 6-35: Removing the Photo Sensor

4.
Install new photo sensor in reverse order of the above steps.
5.
Check plate positioning per Section 7.5.
100-2414D 09/05
6-19
6.3.15 Photo Sensor - Filter Wheel (9310174)

1.
Remove top cover.
2.
Remove cable from photo sensor. Remove screw securing photo sensor and remove (Figure 6-36).
Figure 6-36: Photo Sensor Removal

3.
6-20
Install new photo sensor in reverse order of the above steps.
100-2414D 09/05
6.3.16 Model 680 Light Balancing Filter (9310173)

1.
Remove top cover.
2.
Remove plastic cover and metal cover from atop lamp.
3.
Remove two screws securing lamp housing (Figure 6-37).
Figure 6-37: Lamp Housing

4.
Remove two screws securing the light balancing filter (Figure 6-38).
Figure 6-38: Light Balancing Filter

5.
Install new light balancing filter in reverse order of the above steps.
100-2414D 09/05
6-21
6.3.17 Model 680 XR Light Balancing Filter (9310275)

1.
Remove top cover.
2.
Remove two screws (arrows) securing lamp housing assembly (Figure 6-39).
Figure 6-39: Lamp Housing

3.
Remove two recessed screws securing the light balancing filter (Figure 6-40).
Figure 6-40: Light Balancing Filter

4.
6-22
Install new light balancing filter in reverse order of the above steps.
100-2414D 09/05
6.3.18 Plate Carrier Motor (9310172)

1.
Remove top cover.
2.
Remove the three cover screws and lift away (Figure 6-41).
Figure 6-41: Remove Inner Cover

3.
Remove the two screws from the rail cover and remove cover (Figure 6-42).
Figure 6-42: Cover Screws
100-2414D 09/05
6-23
4.
Loosen the screw to the belt and remove belt Figure 6-43).
Figure 6-43: Loosen Belt Adjustment Screw

5.
Remove two screws securing motor (Figure 6-44). Remove and unplug motor.
Figure 6-44: Motor Mounting Screws

6.
6-24
Install new motor in reverse order of the above steps.
100-2414D 09/05
6.3.19 Optical Fiber Assembly (9310169)

1.
Open top cover.
2.
3.
Open the clip securing the optical fiber assembly. Remove the three screws securing the cover and
remove (Figure 6-45).
Figure 6-45: Removing Optical Fiber Assembly

4.
Remove two screws securing the cover over the analog board
5.
Carefully remove fiber from mounting clips (Figure 6-46).
Figure 6-46: Remove Cover Screws
6.
Install new optical fiber assembly in reverse order of the above steps.
100-2414D 09/05
6-25
7.
When re-installing fiber into filter wheel housing, ensure that end is protruding 2 mm from plate (Figure 6-47).
Figure 6-47: Fiber Installation
6-26
100-2414D 09/05
6.3.20 Filter Wheel (9310170) & Filter Wheel Motor (9310171)

1.
Open top cover.
2.
3.
Cut cable tie and remove two screws securing the filter wheel assembly (Figure 6-48).
Figure 6-48: Filter Wheel Assembly Mounting

4.
Unplug cable from motor to analog board.
5.
Remove two screws securing filter wheel to motor (Figure 6-49).
Figure 6-49: Filter Wheel Mounting

6.
Remove two screws securing motor to mounting bracket.
7.
Install new filter wheel or motor in reverse order of the above steps.
100-2414D 09/05
6-27
6.3.21 Fan (9310168)

1.
Open top cover.
2.
3.
Remove 4 screws and move lamp assembly in order to facilitate unpluging of fan cable (Figure 6-50).
Figure 6-50: Lamp Assembly Mounting

4.
Remove four mounting screws and remove fan (Figure 6-51).
Figure 6-51: Fan Mounting

5.
6-28
Install new fan in reverse order of the above steps.
100-2414D 09/05
6.3.22 Lithium Battery (9006020)

1.
Open top cover.
2.
3.
Replace the lithium battery located on the CPU board (Figure 6-52).
Figure 6-52: Lithium Battery

4.
Install new lithium battery in reverse order of the above steps.
100-2414D 09/05
6-29
Adjustments, Calibration and Checks
Section 7
7.1
Introduction
This section provides the adjustments, calibration and checks applicable to the Model 680 and Model 680 XR
Microplate Readers.
7.2
Tools and Materials
Standard Hand Tools

Voltmeter
9310183
Incubator Adjustment Tool
9310184
RS-232C Cable
1002446
Firmware CD
7.3
Electronics Adjustment
7.3.1
Setting of Jumper on the CPU Board (#281)
1.
Open the top cover and remove plate carrier assembly to access CPU Board (#281).
2.
Locate JP2 (Figure 7-1).
Figure 7-1: Jumper JP2

3.
Set the jumper on the CPU Board (#281) per the following table:
Table 7-1: JP2 Jumper Settings for CPU Board
100-2414E 02/06
Instrument
Configuration
Jumper
Jumper Placement
Without Incubator
JP2
Jump
With Incubator
JP2
No Jumper (Open)
7-1
7.3.2
Setting of the Jumpers on the Printer Control Board (#284)
1.
Open the top cover to access Printer Control Board (#284).
2.
Check printer head type on flexible cable (Figure 7-2).
Figure 7-2: Location of Head Type

3.
Configure the jumpers to JP4 in accordance with the Figure 7-3.
Figure 7-3: JP4 Jumper Settings
7-2
100-2414E 02/06
7.3.3
Incubator Electronics Adjustment (Board #284)
Note: This procedure is used to set the proper temperature set points for the incubator.
1.
Open the top cover and remove plate carrier assembly to access Incubator board (#284).
2.
Disconnect cable from plate carrier to CN52.
3.
Connect cable into Incubation Adjustment Tool, 9310183, 107.79 ohm connector on tool.
4.
Turn on instrument.
5.
Measure voltage between TP5 and TP0 (Figure 7-4).
Figure 7-4: Incubator Board Adjustments

6.
Adjust trim pot VR1 until the voltage measures 0.000V +/- 0.001V.
7.
Connect cable into Incubation Adjustment Tool, 116.47 ohm connector on tool.
8.
Measure voltage between TP5 and TP0.
9.
Adjust trim pot VR1 until the voltage measures 5.528V +/- 0.006V.
10. If voltage is out of range, repeat steps 3 through 9.
100-2414E 02/06
7-3
7.4
Adjusting Plate Carrier Belt Tension

1.
This procedure is used to adjust the belt tension of the plate carrier.
2.
Open the top cover.
3.
Remove fiber optics cover (Figure 7-5)
Figure 7-5: Remove Fiber Optics Cover

4.
Remove two screws securing the belt cover as shown in Figure 7-6.
Figure 7-6: Remove Belt Cover
7-4
100-2414E 02/06
5.
Loosen the screw and adjust the belt tension. Tension should be firm but not overly tight.
Figure 7-7: Belt Tension Adjustment
100-2414E 02/06
7-5
7.5
Alignment of Plate Carrier
7.5.1
Checking Plate Alignment
1.
This procedure is used to check positioning and alignment of the plate carrier.
2.
Open the top cover.
3.
Install Checkmark Plate into reader. Make sure plate is properly seated.
4.
Apply clear tape over small holes in columns 1 and 12 (Figure 7-8).
Figure 7-8: Placement of Tape

5.
Use QC software to move plate beneath optics path per Section 7.7.3.
6.
Check that the light is centered about the holes in the Checkmark Plate in columns 1 and 12 (Figure
7-9).
Figure 7-9: Checking Plate Position at Columns 1 & 12
7-6
100-2414E 02/06
7.5.2
Adjusting Plate Alignment - Y Direction
1.
This procedure is used to adjust the Y direction (top/bottom) position of the plate carrier by repositioning of the rails.
2.
Open the top cover.
3.
Loosen the 12 screws shown in Figure 7-10 and adjust the rails as required.
Figure 7-10: Y Direction Adjustment - Reposition Rails
7.5.3
Adjusting Plate Alignment - X Direction
1.
This procedure is used to adjust the X direction (left/right) position of the plate carrier by repositioning
the photo sensor.
2.
Open the top cover.
3.
Loosen the screws shown in Figure 7-11 and reposition the photo sensor as required.
Figure 7-11: X Direction Adjustment - Reposition Sensor
100-2414E 02/06
7-7
7.6
Firmware
7.6.1
General
1.
Downloading firmware is achieved through a PC (Windows 95, NT, 2000, XP) and an RS-232 cable.
2.
The communication requirements are as follows:

RS232C
Port: COM1
Baud Rate: 9600, 57600
Data: 8 bit 1 stop
3.
The downloading program and additional files are required for downloading:
m680_up.exe
comdlg32.ocx
mscomm32.ocx
comcat.dll
oleaut32.dll
vb6stkit.dll
4.
mscomctl.ocx
msvbvm60.dll
asycfilt.dll
mci32.ocx
olepro32.dll
The current firmware files are:

M680_V171OL.txt for Original Lens models (SN 10001 through 10735)
M680_V171NL.txt for New LCD models (SN 10736 through 13850)
M680_V171.txt for Backlit LCD models (SN 13851 and higher)
M680_V171.txt for Chinese version instruments
M680XR_V163-XR.txt (for Model 680XR readers)
7-8
5.
When updating firmware versions 1.60 and earlier with m680_up.exe, plate reading results, security
password, Checkmark reference values, Checkmark reading results and laboratory name will be lost.
Protocol settings (except for #5-8) and filter wavelengths will be retrieved and restored in the update
process. Date and time will need to be reset.
6.
When updating firmware versions 1.61 and later with m680_up.exe, all data and settings except for
security password, Checkmark reading results, Protocol settings (except for #5-8) and Plate Reading
results (#5-8) be retrieved and restored in the update process. Date and time will need to be reset.
100-2414E 02/06
7.6.2
Firmware History
Table 7-2: Firmware History
Instrument
Style
Firmware
Version
Original LCD
Serial Numbers
10001 - 10735
1.59
Compatibility
History
Original
LCD
New
LCD
Backlit
LCD
Initial release (compatible with serial numbers 10001 through 10735)
Yes
No
No
1.60
Minor corrections (compatible with serial

numbers 10001 through 10735)
Yes
No
No
1.62
Added TSE protocols (compatible with serial

Yes
No
No
1.63
Changed TSE validation criteria (compatible

with serial numbers 10001 through 10735)
Yes
No
No
1.71OL
Added functionality for ESC/P printer.

Added protocols for TSE Sheep/Goat and
Rabies (compatible with serial numbers
10001 through 10735)
Yes
No
No
1.60NL
Released for new LCD display (SN 10736

through 13850). Minor corrections.
No
Yes
No
1.60cNL
Released for Chinese version readers (SN

11201 through 13850).
No
Yes
No
1.62NL
Added TSE protocols (compatible with serial

No
Yes
No
1.63NL
Changed TSE validation criteria (compatible

with serial numbers 10736 through 13850)
No
Yes
No
1.71NL
Added functionality for ESC/P printer.

Rabies (compatible with serial numbers
10736 through 13850)
No
Yes
No
Model 680XR
1.63XR
For Model XR functionality only
No
Yes
No
Backlit LCD
Serial Numbers
13851 and higher
1.70
Released for backlit LCD and integration of

English and Chinese version firmware.
Added functionality for ESC/P printer (effectivity serial number 13851 and higher).
No
No
Yes
1.71

Rabies (effectivity serial number 13851 and
higher).
No
No
Yes
New LCD
Serial Numbers
10736 - 13850
100-2414E 02/06
7-9
7.6.3
Integration of English and Chinese Firmware Versions
Note: With firmware version 1.70, the English and Chinese versions have been integrated. A language choice
has been added with this version.
1.
To enter the Select Language screen, press the 0 key and 4 key and turn ON instrument power.
Hold for approximately 5 seconds and the Select Language screen will appear.
2.
Use Up and Down arrow keys to select language choice. Press Enter.
3.
Reader resets automatically and restarts with the selected language.
Note: If the language selected is different, the reader will clear all memory and restore default values.
7-10
100-2414E 02/06
7.6.4
1.
Downloading Firmware
Load the m680_up.exe program. The screen shown in Figure 7-12 will appear.
Figure 7-12: Microplate Reader Model 680 Update Screen

2.
Click on Model 680 Update and the following screen will appear:
Figure 7-13: Click Yes after Log-in

3.
Connect a RS-232C cable between Model 680 and PC COM1 port.
4.
Turn ON Model 680 reader and input password for log in (default 00000). Press enter.
5.
When complete, press Yes button on screen.
100-2414E 02/06
7-11
6.
The following screen will appear:
Figure 7-14: Confirmation Screen

7.
Press Yes to update to new version. If the instrument is at the latest version, the screen will indicate
no update is required.
8.
The screen shown in Figure 7-15 will indicate the status as existing data and settings are retrieved
from the Model 680 reader.
Figure 7-15: Retrieving Data Screen
7-12
100-2414E 02/06
9.
When data retrieving is complete, the following screen will appear:
Figure 7-16: Firmware Update Screen

10. The following steps are also indicated on the screen shown in Figure 7-16
11. Turn OFF the Model 680 Reader.
12. While pushing both Main and 0 key on the keypad, turn on reader power switch. After about 10
seconds, the following screen on LCD appears:
Firmware update
Are you sure?
Yes: Start Key
No: 7 Key
13. Push Start/Stop key switch on the Model 680 reader to begin reader firmware erasing.
14. After the erasing finishes, the LCD screen RS Receive will appear.
15. Click the Start button on PC screen. The firmware will begin uploading and bar graph at the bottom
of the screen will display status. This will take approximately 7 minutes.
16. When the firmware update operation is complete, the LCD on the reader will displayed Finished
and the restore previous data screen will appear (Figure 7-17).
100-2414E 02/06
7-13
Figure 7-17: Restore Previous Data Screen

17. Turn OFF reader power switch then turn ON. A Battery Low error message will occur. This is normal; press Enter.
18. At the Calendar settings screen, enter Date and Time and press Enter.
19. Enter password and press Enter.
20. After completion of log-in press the Yes button on the PC screen.
21. The restoring data screen will appear (Figure 7-18).
Figure 7-18: Storing Data Screen
7-14
100-2414E 02/06
22. When the update is complete, the firmware complete message will appear (Figure 7-19).
Figure 7-19: Firmware Update Complete

23. Click OK to continue.
24. A password change reminder screen will appear (Figure 7-20).
Figure 7-20: Change Password Reminder Screen

25. Follow on screen instructions to change password or select Exit to quit.
100-2414E 02/06
7-15
7.7
QC Software
Note: There are 4 modes in this program; Measure Mode, Monitor Mode, Extension Mode and Incubator. The
instructions in this section apply to the Monitor Mode which allows step-by-step positioning of the plate carrier.
7.7.1
Entering QC Software
1.
While pressing the 0 and Upper key buttons, turn ON power switch. Continue to hold down buttons until QC Software screen appears.
2.
Select YES by pressing the 1 key.
3.
Input the password 00000 and press Enter.
4.
Press the Dot key . to enter the QC Mode menu (Figure 7-13).
QC
1: Measure Mode
2: Monitor Mode
3: Extension Mode
4: Incubator
Figure 7-21: QC Mode
7.7.2
1.
Monitor Mode
Select Monitor Mode from the menu by pressing 2 (Figure 7-14).
MON:
V
DEC:
CH: A PLT: 12
FL: 1 Gain=1.1.1.0
Figure 7-22: Monitor Mode
Definitions:
MON = AD Value (voltage conversion)
DEC = AD Value (decimal)
CH = Row Position
PLT = Plate Column Position
FL = Filter Number
Gain = Gain A, B
Commands:
Main = Back to QC Mode Screen
Change of Gain A
Change of Gain B
Change of Plate Position
Change of Filter Position
. (Dot) = Change of Channel
7-16
100-2414E 02/06
7.7.3
Plate Positioning
1.
To use the QC software to check for plate positioning, enter the QC Mode and press 2 for Monitor
Mode.
2.
The reader will initialize the plate carrier and place column 12 in the optics path.
3.
Press the down arrow key to change filter position for desired filter position.
4.
Press the up arrow key to step the plate through column positions 11 to 1.
7.8
External Printer Setup
Note: The Model 680 Reader with firmware version 1.70 added support for ESC/P external printer mode.
Previous versions of firmware support only ASCII print mode.
1.
To change the external printer print mode, perform the following steps:
a. Select Edit from the top screen menu
b. Select Printer
c. Select desired print mode: ASCII or ESC/P
d. Press Enter
2.
Connect printer and check print application by performing any run.
100-2414E 02/06
7-17
Troubleshooting
Section 8
Troubleshooting
8.1 About this Section
This section of the manual provides information on troubleshooting the Model 680 and 680 XR Microplate
Reader. The information in this section will help diagnose the common operating difficulties and component
failures. If additional assistance in resolving a problem is required, please contact the Technical Service
Department at 1-800-424-6723.
8.2 Troubleshooting
The following troubleshooting tables are intended as a guide to assist in diagnosing some common problems.
Table 8-1: General Problems
Problem
Will not communicate with computer
Possible Cause
Solution
Improper COM Port selected
Check that COM1 port is used.
Improper serial cable
Check for proper serial cable .
CPU board problem
Replace CPU board.
Fuse Blown
Check Fuse. If bad, replace
Cable connection
Check display cable and reseat.
Bad LCD
Replace LCD.
Memory Error
Bad CPU Board
Replace CPU Board.
Calendar Error
Data error
Reset time and date. Reboot.
Bad CPU Board
Replace CPU Board.
Printer malfunction
Replace printer.
Will not print to external printer
Check printer setup Section 7.8

(ESC/P requires firmware version 1.70 or later)
Battery dead
Replace battery.
Old version firmware
Download new firmware.
LCD Hardware Error
LCD malfunction
Replace LCD.
A/D Conversion Error
Loose cable connection
Check cables to CPU board and

Analog Board
Bad A/D convertor
Replace Analog Board.
Carrier binding
Check for source of binding.

Clean and lubricate plate carrier
rods as necessary.
Blank LCD upon Power Up
Printer Error
Battery Error
Grinding Sound or Plate Error
100-2414E 02/06
8-1
Troubleshooting
Table 8-2: Error Codes

Problem
Possible Cause
Solution
Code 8071- Invalid Command
Received data error
Resend command
Code 8072 - Parameter Out of

range
Received command parameter is

out of range
Resend command and parameter
Code 8073 - Not in Remote

Mode
Instrument is not in Remote

Mode
Send the AQ command to

acquire Remote Control Mode
Code 8074 - Device Busy
Instrument is busy
Wait until ready
Code 8075 - Filter Disk Jammed
CPU board bad
Replace CPU board
Motor bad
Replace motor
Photo interrupter for Filter disk

damaged
Replace photo interrupter
Filter disk damage
Replace the filter disk
Code 8076 - Carrier Jammed

After Reading
Plate is not properly seated
Reseat plate
Interference or blockage
Check for Interference or blockage
Code 8077 - Light Bulb Error
Lamp defective
Replace lamp
Lamp out of alignment
Check that lamp is properly

seated
Optical fiber damaged
Replace optical fiber
Bad analog board
Replace Analog Board
Code 8082 - Filter Wheel
Filter wheel jammed
Check filter wheel
Code 8083 - Incubator Error
Thermo sensor damaged
Replace plate carrier
Incubation control board bad
Replace Incubation control board
Photo interrupter for plate carrier

damaged
CPU board bad
Replace CPU board
Reseat plate
Interference or blockage
Check for Interference or blockage
Photo interrupter for plate carrier

damaged
CPU board bad
Replace CPU board
Motor bad
Replace motor
Code 8084 - Carrier Home Sensor Closed Status Error
Code 8085 - Carrier Home Sensor Open Status Error
8-2
100-2414E 02/06
Troubleshooting
Table 8-2: Error Codes

Problem
Code 8086 - Carrier Jammed
Before Reading
Possible Cause
Solution
Reseat plate
CPU board bad
Replace CPU board
Motor bad
Replace motor
Code 8087 - Plate Carrier Position
Plate carrier jammed after reading
Check plate carrier for obstruction.
Code 8088 - Light Level Error

Between Channel
Dirty Lenses
Clean lenses
Optical fiber damaged
Replace optical fiber
Bad analog board
Code 8089 - Filter Missing
Filter missing from filter wheel

allowing light to exceed acceptable range
Check filter wheel and install filter
Code 8090 - Security Code in

Error
Security code did not match
Remove security lock
Code 8091 - RS-232C Error
Data communication problem
Check RS-232 cable
Code 8092 - A/D Conversion

Error
Bad A/D convertor
Code 8093 - Stop Key Pressed
Stop key pressed during measurement
User error. Reset command
100-2414E 02/06
8-3
Troubleshooting
Table 8-3: Firmware Downloading

Problem
Possible Cause
Solution
Checksum Error
Corrupted Firmware File
Repeat using uncorrupted file
Communication Time Out
RS-232 not connected
Ensure RS-232 is properly connected.
Wrong button clicked
Repeat download procedure
RS-232C Error
Parity, Overrun or Framing errors

occurred
Ensure RS-232 is properly connected. Repeat download procedure
Verify Error
Communicated data and written

data is different

(make sure instrument power is
cycled)
Flash Write Error
Flash memory could not be written

cycled)
Flash Erase Error
Flash memory could not be

erased

(cycle power; first screen to
appear is downloading screen)
Erase Check Error
No erasing memory exists

cycled)
Battery Error
Occurs after firmware download
Normal. Press Enter. Reset date,

time and filter data.
8-4
100-2414E 02/06
Troubleshooting
Table 8-4: Checkmark Plate Failures

Problem
Fails Accuracy, Linearity or
Precision
Possible Cause
Erratic or non-uniform well-to-well
OD values
Solution
Check for cleanliness of optics
and lenses. Clean as required.
Check lamp. Reseat or replace
as necessary.
Check filter. Reseat or replace as
necessary.
Replace fiber optics assembly
Replace analog board
Replace CPU board
Fails Plate Positioning
Failed Cross Talk
Plate binding
Check for proper movement of

plate.
Belt tension too tight or too loose
Check belt tension and tighten/

loosen as necessary per Section
7.4
Plate carrier out of alignment
Check plate alignment and correct as necessary per Section 7.5
Wells A2, C2, E2, G2, B3, D3, F3,

and H3 are exceeding 0.003 OD
due to some light path blockage.
Check for cleanliness of optics

and lenses. Clean as required.
Check lamp. Reseat or replace
as necessary.
Check filter. Reseat or replace as
necessary.
Check for proper movement of
plate.
Check belt tension and tighten/
loosen if necessary per Section
7.4
Replace fiber optic assembly
Fails Filter Check
Filter deterioration
Replace filter
Fails 0 OD/Overflow
Not detecting infinite absorbance

Replace analog board
Fails IR Blocking
100-2414E 02/06
Detected IR light passing through

plate; not blocked by filters
Check filter for damage or deteriation. Replace as necessary.
8-5
Parts Information
Section 9
Parts Information
This section of the manual provides information on the field replaceable parts and service tools for the Model
680 and 680 XR Microplate Readers. Information on parts and tools ordering is also included in this section.
9.2 Parts Orders

9.2.1 US Domestic Operations
Parts may be ordered through our Service Parts department. You can reach the Service Parts department
during Pacific time regular office hours by calling 1-510-741-5038 or 5058. You can help to make processing
your order more efficient by having the following information before you call:
The part number(s) or catalog number(s) of the parts you want to order
Shipment information
Your account or inventory location number
9.2.2 International Subsidiaries

The procedures for ordering parts vary from region to region outside the United States. Please contact your
Bio-Rad Export Sales Representative through your regular sales and service channels.
9.3 Returning Defective Parts

9.3.1 US Domestic Operations
Defective Assemblies returned for evaluation and repair are an important part of the logistics and inventory
control process. Returned parts and assemblies are refurbished when possible, tested and returned to service stock. Maintaining the effectiveness and efficiency of this process enables us to deliver the high standard
of service that our customers expect, at a reasonable cost that allows us to remain competitive.
You are an integral part of our inventory process. When you return parts or assemblies to Bio-Rad to be evaluated and repaired, please attach a tag to each assembly. The tag should contain the following information:
Model and serial number of the unit which the part was removed
The part number or catalog number of the part being returned
The date of removal
A description of the problem or symptoms exhibited
Your name and other identification information
This information will help our service centers to make repairs efficiently. When you receive replacement parts,
save the packaging material for returning the defective parts for repair.
100-2414E 02/06
9-1
Parts Information
9.3.2 International Subsidiaries

The procedures for returning parts vary from region to region outside the United States. Contact your Bio-Rad
Export Representative through your regular service channels.
9-2
100-2414E 02/06
Parts Information
9.4 Service Parts

The following is a list of service parts for the Model 680 Microplate Reader.
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Description
Part Number
Battery, Lithium, CR2032, 3V
900-6020
Cable, Serial, RS-232C
931-0184
Cover Assembly, Top (Model 680)
931-0161
Cover Assembly, Top (Model 680 XR)
931-0333
100-2414E 02/06
9-3
Parts Information
Description
9-4
Part Number
Cover Assembly, Top (Model 680

Chinese)
9310230
Cover, Bottom
9310162
Cover, Rear
9310163
Door
9310164
Fan
9310168
100-2414E 02/06
Parts Information
Description
Part Number
Fiber Holder
9310274
Filter Wheel
9310170
Fuse, T3.15A, 250V, 20mm x 5mm
9000231
Inlet Assembly
9310167
Keypad Membrane (Model 680)
9310177
100-2414E 02/06
9-5
Parts Information
Description
9-6
Part Number
Keypad Membrane (Model 680 XR)
9310277
Keypad Membrane (Model 680

Chinese Keypad))
9310229
LCD Display Assembly (Note: for serial

numbers 10001 to 10734, the CPU board
9310225 must also be ordered)
9310224
LCD Backlit Display Assembly (Note: for

serial numbers 13851 and higher)
9310351
LCD Backlit Display Kit - includes new

hardware (Note: for retrofitting serial
numbers 10001 to 13850; the CPU board
9310353 must also be ordered)
9310352
100-2414E 02/06
Parts Information
Description
Part Number
LCD Display Assembly (Model 680 XR)
9310332
Lens Block
9310273
Light Balancing Filter, Model 680
9310173
Light Balancing Filter, Model 680 XR
9310275
Motor, Filter Wheel
9310171
100-2414E 02/06
9-7
Parts Information
Description
9-8
Part Number
Motor, Plate Carrier
9310172
Optical Fiber Assembly
9310169
Optical Assembly (Model 680 XR)
9310331
PCB, Analog Board (#282), Model 680
9310182
PCB, Analog Board (#301), Model 680

XR
9310289
100-2414E 02/06
Parts Information
Description
Part Number
PCB, CPU Board (for Model 680 serial

numbers 10001 to 10735)
9310181

numbers 10736 to 13850)
9310225

numbers 13851 and above)
9310353
PCB, CPU Board (for Model 680 XR)
9310278
PCB, Incubation Control Board (#286)
9310180
100-2414E 02/06
9-9
Parts Information
9-10
Description
Part Number
PCB, Lamp Control Board, Model 680 XR
9310276
Photo Sensor, Plate Carrier
9310174
Photo Sensor, Filter Wheel
9310348
Plate Carrier Assy without Incubator
9310175
Plate Carrier Assy with Incubator
9310176
100-2414E 02/06
Parts Information
Description
Part Number
Power Supply, 12 Volt
9310178
Power Supply, 24 Volt, Incubator
9310179
Printer Assembly
9310160
Rail, Right
9310165
Rail, Left
9310166
Shield Cover, CPU PCB
9310360
100-2414E 02/06
9-11
Parts Information
Table 9-2: Consumables

Description
9-12
Part Number
Lamp, Model 680, 12V, 20W
1681006
(9310152)
Lamp, Model 680 XR, 6V, 20W
1681104
(10000562)
Paper Roll
1681007
(9310153)
100-2414E 02/06
Parts Information
9.5 Filters
The following is a list of custom filters available:
Table 9-3: Filters
Wavelength
Part Number
Wavelength
Part Number
340 nm*
345 nm*
350 nm*
355 nm*
360 nm*
365 nm*
370 nm*
375 nm*
380 nm*
385 nm*
390 nm*
395 nm*
400 nm
405 nm
410 nm
415 nm
420 nm
425 nm
430 nm
435 nm
440 nm
445 nm
450 nm
455 nm
460 nm
465 nm
470 nm
475 nm
480 nm
485 nm
490 nm
495 nm
500 nm
505 nm
510 nm
515 nm
520 nm
525 nm
530 nm
535 nm
540 nm
545 nm
550 nm
555 nm
560 nm
565 nm
570 nm
1681105
1681106
1681107
1681108
1681109
1681110
1681111
1681112
1681113
1681114
1681115
1681116
1681010
1681011
1681012
1681013
1681014
1681015
1681016
1681017
1681018
1681019
1681020
1681021
1681022
1681023
1681024
1681025
1681026
1681027
1681028
1681029
1681030
1681031
1681032
1681033
1681034
1681035
1681036
1681037
1681038
1681039
1681040
1681041
1681042
1681043
1681044
575 nm
580 nm
585 nm
590 nm
595 nm
600 nm
605 nm
610 nm
615 nm
620 nm
625 nm
630 nm
635 nm
640 nm
645 nm
650 nm
655 nm
660 nm
665 nm
670 nm
675 nm
680 nm
685 nm
690 nm
695 nm
700 nm
705 nm
710 nm
715 nm
720 nm
725 nm
730 nm
735 nm
740 nm
745 nm
750 nm
755 nm*
760 nm*
765 nm*
770 nm*
775 nm*
780 nm*
785 nm*
790 nm*
795 nm*
800 nm*
1681045
1681046
1681047
1681048
1681049
1681050
1681051
1681052
1681053
1681054
1681055
1681056
1681057
1681058
1681059
1681060
1681061
1681062
1681063
1681064
1681065
1681066
1681067
1681068
1681069
1681070
1681071
1681072
1681073
1681074
1681075
1681076
1681077
1681078
1681079
1681080
1681117
1681118
1681119
1681120
1681121
1681122
1681123
1681124
1681125
1681126
*Model 680 XR only
100-2414E 02/06
9-13
Parts Information
9.6 Tools
The following is a list of special tools and software required for servicing the Model 680 Microplate Reader.
Table 9-4: Special Service Tools and Firmware
Tool Description
9-14
Part Number
Incubator Adjustment Tool
9310183
Checkmark Reader Performance

Check Plate
1706940
Firmware
1002446
100-2414E 02/06
Parts Information
9.7 Manuals
The following is a list of reference manuals.
Table 9-5: Manuals
Tool Description
Microplate Manager Version
5.2.1 Software Users Manual
Part Number
1709520
(Parallel Port Hasp)
1709523
(USB Hasp)
Checkmark Reader Performance

Software Instruction Manual
100-2414E 02/06
4000186
9-15
Drawings
Section 10
Drawings
This section contains drawings for the Model 680 and 680 XR Microplate Reader.
10.2 Drawings
Block Diagram
Wiring Diagram
100-2414C 03/04
10-1
Model 680 Block Diagram

Interference Filters
Color Conversion Filter
Halogen Lamp
Motor
Plastic Fiber
Refrence
AC Input
Convex Lenzs
Microplate
Heater & Pt Sensor
(option)
Motor
Ball Lenzs
Analog Board
( Amplifier )
( A/D converter )
Power Supply
RS232C
Fan
Main Board
( CPU controller )
( Motor driver )
Printer Port
Power Supply
(Option)
Internal
Printer
(Option)
Incubation
(Option)
LCD & Key Switch
Photo Diodes
Inlet
Fuse
5p
CN1
Power Supply
12V
CN2
4p
Power SW
No.283-2
Option
Lamp
Dsub 25p
Internal
Printer
CN43
Membrane SW
Dsub 9p
LCD
CN22
CN44
30p
25p
CN45
Printer
Control
Board
12p
CN62
No.284
20p
CN63
No.283-1
Fan
CN61
34p
6p
M1
M2
Shield Case
(Filter)
CN08
3p
CN07
PI1
CPU Board
Power Supply
24V
(Plate Carrier)
7p
34p
CN06
(Plate Carrier)
4
PI2
4p
5p
2p
CN33
No.281
4p
CN2a
CN1a
3p
CN01
No.286
CN53
2p
9p
CN02
CN11
10p
26p
CN04
CN05
CN51
30p
CN42
CN09
5p
CN52
5p
No.286-2
Heater
CN54
Incubation
Control
Board
CN10
Option
PT Sensor
34p
(Filter)
34p
A
CN81
Base Plate
No.282
Measurement
C
D
Analog Board
Photo
Diodes
Reference
Title
F
G
Document Number
$4'
Date:
/QFGN/KETQRNCVG4GCFGT
9KTKPI&KCITCO
June 4, 2001
Rev
;CUWUJK-QEJK[COC
Sheet 1 of
5
Appendix
Appendix
Appendix A - Model 680 System Checkout Report
Appendix B - Model 680 XR System Checkout Report
Appendix C - Checkmark Check Plate
Appendix D - Error Flow Diagrams
100-2414C 03/04
Appendix A

System Checkout Report
Customer:
Instrument Serial Number:
Case Number:
AC Input Voltage:
Test
Configuration
Specification
Results
168-1000 (No Temp Control, No Internal Printer)
N/A
168-1001 (With Temp Control, No Internal Printer)

168-1002 (No Temp Control, With Internal Printer)
168-1003 (With Temp Control, With Internal Printer)
Firmware
Firmware Version
Instrument Function
System Checkout
Record__________
Pass
Fail
Keypad and Display

Printer Function (if applicable)
Self Diagnosis and Start Up
Mixing Feature
Incubator (if applicable)
Pass
Fail
Air Read (all wells < 0.005)
Pass
Fail
Accuracy (CV 5.0%)
Pass
Fail
Channel-to-Channel (CV 5.0%)
Pass
Fail
Stability and Drift (< 0.010 OD)
Pass
Fail
The above referenced instrument has been tested and meets all performance specifications.
______________________________
Bio-Rad
_____________
Date
Appendix B

System Checkout Report
Customer:
Instrument Serial Number:
Case Number:
AC Input Voltage:
Test
Configuration
Specification
Results
168-1100 (No Temp Control, No Internal Printer)
N/A
168-1101 (With Temp Control, With Internal Printer)
Firmware
Firmware Version
Instrument Function
System Checkout
Record__________
Pass
Fail
Keypad and Display

Printer Function (if applicable)
Filter Installation
Self Diagnosis and Start Up
Mixing Feature
Incubator (if applicable)
Pass
Fail
Air Read (all wells < 0.005)
Pass
Fail
Accuracy (CV < 5.0%)
Pass
Fail
Channel-to-Channel (CV < 5.0%)
Pass
Fail
Stability and Drift (< 0.010 OD)
Pass
Fail
The above referenced instrument has been tested and meets all performance specifications.
______________________________
Bio-Rad
_____________
Date
Appendix C
Appendix C
Checkmark Reader Performance Check Plate
The Checkmark program is used with the Checkmark Reader Performance Check Plate for validating the
performance of the Model 680 Microplate Reader. It will test reader accuracy, precision and linearity. In
addition it will provide information on plate positioning, cross-talk, filter wavelength, overflow and 0 OD, and
infrared (IR) blocking for diagnostic purposes.
The Bio-Rad Catalog number is 170-6940. The Checkmark program and Check Plate is manufactured by:
Anthos Labtec Instruments
Lagerhausstrasse 507
A-5071 Wals/Salzburg, Austria
Tel: +43 (0) 662 857 220
Fax: +43 (0) 662 857 223
e-mail: anthos@anthos-labtec.com
Web Site: www.anthos-labtec.com
100-2414C 03/04
C-1
Appendix D Error Flow Diagrams

Hardware error detection upon power turn on.
Memory error
Calendar error
Printer error
Battery error
LCD hardware error
Turn
on
the
power switch
Self-diagnosis.
D/A conversion error
A/D conversion error

Error code : 8092
Carrier home sensor open status error
Error code : 8085
Carrier home sensor closed status error
Error code : 8084
Carrier jammed error before reading
Error code : 8086
Carrier jammed error after reading
Error code : 8087
Filter disk jammed
Error code : 8075
Instrument displays error message

on LCD.

Hardware error detection upon plate read.
Printer error
Light level error between cannel

Error code : 8088
Light bulb error
Error code : 8077
D/A conversion error
A/D conversion error

Error code : 8092
Plate reading
Carrier home sensor open status error

Error code : 8085
Carrier home sensor closed status error
Error code : 8084
Carrier jammed error before reading
Error code : 8086
Carrier jammed error after reading
Error code : 8087
Filter disk jammed
Error code : 8075
Filter missing
Error code : 8089

on LCD or returns error code to
PC.

Hardware error detection upon incubator activation.
Incubator
activated
Incubator error
Error code : 8083.

on LCD or returns error code to
PC.

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Life Science Group

Model 680 and 680 XR

Part Number 100-2414 Revision E

Copyright 2002-2006 by Bio-Rad Laboratories, Incorporated. All rights reserved.

About this manual

document is part of Life Science Groups commitment to provide

We welcome and appreciate any suggestions you may have to improve

Revision Control List

Updated Checkmark Plate procedure

Added optics cleaning procedure

Added part numbers to sections; Updated Figure 6-9

Added Checkmark Plate; Updated Figure 7-3

Added Section Alignment of Plate Carrier

Added Table 8-4 Checkmark Plate Failures

Manual Updated to Add Model 680XR

Added 6.3.3 Model 680 XR Lamp

Added Table 6-1

Added 6.3.17 Model 680 XR Light Balancing Filter

Added Table 2 Firmware History

Updated Table 8-1

Added check of plate position

Updated parts information

Added Backlit LCD to Table 6-1

Updated to reference Section 7.7

Updated Firmware Table 7-2 and added 7.6.3

Added Section 7.7 QC Software

Updated Firmware version 1.71; Table 7-2 update

Updated 7.6.4 Firmware Downloading

Added Section 7-8 External Printer Setup

Updated troubleshooting printer

Added Shield Cover

Section 2 System Overview

Memory Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

RS-232 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

2.6 Instrument Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

Section 4 System Checkout

Preparing Yellow Dye Test Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

Touch Pad/Mixing Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

Verify Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

Start Microplate Manager . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

Air Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5

4.4.10 Channel-to-Channel Variation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8

Section 5 Preventative Maintenance

Inspection of Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

Perform System Checkout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

Section 6 Removal and Replacement

Interference Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

Model 680 Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

Model 680 XR Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3

Top Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4

Printer Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5

Keypad Membrane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-7

Inlet Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-8

Plate Carrier Assembly and Analog Board (#282) . . . . . . . . . . . . . . . . . . . . . . . . 6-9

CPU Board (#281) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-12

6.3.10 Incubator Control Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-13

6.3.15 Photo Sensor (Filter Wheel) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-20

Section 7 Adjustment and Calibration

Setting of Jumper on the CPU Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1

Setting of Jumpers on the Printer Control Board . . . . . . . . . . . . . . . . . . . . . . . . . 7-2

Incubator Electronics Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3

7.4 Adjusting Plate Carrier Belt Tension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4