Access Interfaceamento

Immunoassay System
LIS Vendor Information
Revision 101968X
Revised Aug 2005
Printed in U.S.A.
2005 Beckman Coulter, Inc.
BECKMAN COULTER, INC. FULLERTON, CA 92835
Publication Notes
Each page of this manual is identified with its revision and release date. For pages other than
the title page, revision information is located at the bottom of the page.
This manual includes pages from three revision releases.
The pages listed below are issued as revision 101968X, release date 8/05.
Title page and Publication Notes
Pages 21 through 24
The pages listed below were issued as revision 101968T, release date 1/02:
Pages 12 and 14
All other pages in this manual are issued as revision 101968P, release date 3/00.
This manual is intended for use with the Access Immunoassay System analyzer.
Ostase is trademark of Hybritech Incorporated, a subsidiary of Beckman Coulter, Inc.

Access, AccuTnI, Hybritech, and the BECKMAN COULTER logo are trademarks of
Beckman Coulter, Inc.
Table of Contents
Table of Contents
Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Frame Layer Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Physical Layer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Message Layer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Record Field Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Test Codes Used by the Access System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Sample Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Limits and Performance Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
2000 Copyright Beckman Coulter, Inc.
Access LIS Vendor Information
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Table of Contents
ii
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This document is intended as a guide to LIS vendors developing interfaces
that communicate with the Access Immunoassay System. Provide this
information to your LIS vendor prior to interfacing the Access System and
the LIS.
This document is based on the following ASTM standard protocols:
ASTM E1394-91
ASTM E1381-95
Refer to Appendix A, LIS Interface, of the Access Reference Manual for
information on configuration and use of the Access System LIS interface.
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Definitions
Definitions
The following is a list of terms used in this document and a definition of each
term as it is used by Beckman Coulter, Inc.
Term
Definition
<ACK>
Acknowledgment (ASCII Decimal 6).
[C1]
The most significant character of Checksum.
[C2]
The least significant character of Checksum.
[DATA]
The data contents of the record.
<ENQ>
Inquire (ASCII Decimal 5).
<ETB>
End of Transmission Block (ASCII Decimal 23). For use

only when a single record is too large to fit into one frame.
<ETX>
End of Text (ASCII Decimal 3). Required at the end of

each record.
[frame number]
Single digit frame number 0 to 7. Starts with 1.
<LF>
Line Feed (ASCII Decimal 10).
<NAK>
Negative Acknowledgment (ASCII Decimal 21).
<STX>
Start of Frame (ASCII Decimal 2).
Communications
packet
All framing required for transmission of data. This

framing includes: <STX>[frame number][DATA] [<ETB>
or <ETX>][C1][C2] <LF>.
Component Field
One of several related pieces of information within a field.
Download
The transmission of data from the LIS to the Access

System.
Field
A specific location within a record for a piece of

information, indicated by a field delimiter and position.
Frame
A complete communications packet.
LIS
Laboratory Information System.
Message
A collection of related information; a group of records

that begins with a Header record and ends with a
Terminator record. A single record could theoretically
constitute a message, but within this context, a message
always contains multiple records. Refer to the Message
Layer section and the descriptions of each type of record
included later in this document.
Receiver
The device that responds to the sender. The receiver in

this document is either the Access System or the LIS.
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Definitions
Term
Definition (continued)
<EOT>
End of Transmission (ASCII decimal 4).
<CR>
Carriage Return (ASCII decimal 13).
Record
In reference to the low level protocol, a record is the

message data (shown as [DATA]) as described within the
communications packet. If the data is longer than 240
characters, then it must be split into two parts and sent in
two communications packets. The intermediate packet
uses the <ETB> character, and the ending packet uses the
<ETX> character. No single communications packet
contains more than one record.
In reference to the message layer, a record can be one of
the following codes: H (header), P (patient), O (order),
R (result), L (terminator), C (comment). Refer to the
Message Layer section later in this document.
Repeat Field
An additional field of the preceding type when indicated

by a repeat delimiter. The Access System parses and may
produce repeat fields in the Universal Test ID field of a
Test Order Record, but ignores other occurrences of repeat
fields.
Sender
The device that has a message to send and initiates the

transmission process, in this case between the LIS and the
Access System. The sender in this document is either the
Access System or the LIS.
Session
A total unit of communication activity used in this

standard to indicate the events starting with the
Establishment phase and ending with the Termination
phase. Refer to Frame Layer Protocol later in this
document.
Test
A determination of a single analyte or a combination of

values from other determinations or observations from
which a variable or gradable result is derived.
Upload
The transmission of data from the Access System to the

LIS.
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Frame Layer Protocol
Frame Layer
Protocol
The recommended Low Level Protocol to use for transferring messages is based
on an ACK/WAIT protocol. The low level protocol transfers messages as
specified by the ASTM E1381-95 standard.
Establishment Phase
During the establishment protocol phase, the communication link between the
Access System and the LIS is set up.
Receiver State
Sender
Receiver Ready
<ENQ> -->
<-- <ACK>
Receiver Not Ready
<ENQ> -->
<-- <NAK>
Receiver
After an <ENQ> is sent, the state of the sender must then change to allow data to
be received.
If both the Access System and the LIS send an <ENQ> simultaneously, the Access
System has priority.
Transfer Phase
During the transfer protocol phase, data is sent back and forth between the Access
System and the LIS. The Access System uses data transfer conventions outlined in
the ASTM E1381-95 standard for the data layer protocol. Any conventions that are
not supported are noted and addressed. Refer to the Message Layer section later
in this document.
Termination Phase
During the termination protocol phase, a sequence of characters or conditions
causes communication between the Access System and the LIS to cease, either
normally or abnormally.
Transport Layer Sequence

The following tables illustrate a transport layer sequence:
Sender
Session Description
Normal Session:
<ENQ> ->
<<STX> [F1] [DATA] <ETX> [C1] [C2] <CR> <LF> ->
<<EOT> ->
Receiver
<ACK>
<ACK>
No Response
Expected
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Frame Layer Protocol
Session Description
Sender
Receiver
Failure Session (NAK):
<ENQ> ->
<-
<NAK>
(Delay 10 seconds)
(Repeat up to 6 times)
(Before 6 <NAK>s)
<ENQ> ->
<<EOT> ->
<ACK>
<ACK>
No Response
Expected
Session Description
Sender
Failure Session (Max <NAK>s):
<ENQ> ->
<<ENQ> ->
<<EOT> ->
(Delay 10 seconds)
(After 6 <NAK>s)
Receiver
<NAK>
<NAK>
No Response
Expected
Session Description
Sender
Failure Session (No Response):
<ENQ> ->
(Time-out after 15 seconds)
<EOT> ->
Receiver
No Response
No Response
Expected
Session Description
Sender
<ENQ> ->
<- <ACK>
<STX> [F1] [DATA] <ETX> [C1] [C2] <CR> <LF> ->

<(Delay 10 seconds)
(Before 6 <NAK>s)

<<EOT> ->
Receiver
<NAK>
<NAK>
<ACK>
No Response
Expected
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Physical Layer
Session Description
Sender
Receiver
<ENQ> ->
<-
<ACK>

(Delay 10 seconds)
<NAK>
<- <NAK>
(After 6 <NAK>s)
<EOT> ->
No Response
Expected
Physical Layer
All communications are expected to use the RS232 communication protocol,

based upon the Electronics Industries Association (EIA) standard RS232-C.
As part of the conformance to this standard, the Access System is configured as
Data Terminal Equipment (DTE).
The Access System is cabled to the LIS via the DB-9-M or DB-9-F connector on the
Access System (labeled LIS; located near the printer and terminal ports).
The DB-9 Connector provides RXD at pin 2, TXD at pin 3, and signal ground at
pin 5. No other connections are used for the ASTM E1381-95 protocol. The
following table shows the pin assignments for both the Access System and the
LIS.
Pin
Access LIS Port

Configuration
LIS Cable Must Provide
RXD
TXD
TXD
RXD
Ground
Ground
The operator defines the baud rate, choosing either 1200 or 2400 baud. The
number of data bits per character, parity bit, and number of stop bits are as
defined in ASTM standard E1381-95.
Message
Layer
This document references the ASTM standard E1394-91, and the recommended
support of the ASTM protocol. The intent of this section is to develop a complete
understanding of the particular records and fields as supported by the Access
System.
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Message Layer
The low level protocol communications are separate from the message level. It is
recommended that the ACK/WAIT protocol specified in the Frame Layer
Protocol section be used with this ASTM standard.
Message Content
The following is a list of the limitations and general considerations regarding
message content.
Allowed
Characters
Allowed are ASCII 7 (BEL), 9 (HT), 11 (VT), 12 (FF),

13 (CR), 32-126, 128-254. However, the message data
(shown as [DATA]) sent to the Access System must be
restricted to ASCII 32-126 for proper operation of the
Access System Software. Specific fields may further
restrict allowed characters.
Maximum Field
Length
No maximum field length is imposed within the

message-receiving mechanism. However, the message
parsing performed by the Access System/LIS Interface
Software enforces certain restrictions. Refer to the record
tables later in this document for specific field
restrictions.
Maximum Record
Length
No maximum record length is imposed within the

message-receiving mechanism. However, the message
parsing performed by the Access System Software
restricts records to 1024 characters in length.
Delimiters
Any of the allowable characters, as specified in ASTM

E1394-91, may be used as delimiters. Unless otherwise
documented, the following characters should be used:
Field delimiter
=
vertical bar (|)
Repeat delimiter
=
backslash (\)
Component delimiter =
caret (^)
Escape delimiter
=
ampersand (&)
Record Codes
The following codes are required in relation to the

ASTM standard:
Header Record
H
Patient Record
P
Test Order Record
O
Result Record
R
Comment Record
C
Scientific Record
S
Manufacturer Record
M
Request Information Record Q
Final Record
L
Fields with Null

Values
Nulls are sent when data need not be updated. A null

value does not conflict with existing data in most cases.
Erroneous data is the responsibility of the sender.
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Record Field Contents
Record Field
Contents
Manufacturer
Information
Record
The Manufacturer Information Record is ignored when

received by the Access System and not created or sent
by the Access System.
Scientific Record
The Scientific Record is ignored when received by the

Access System and not created or sent by the Access
System.
This section lists the following records and the fields they contain:
Message Header Record
Patient Information Record
Test Order Record
Result Record
Comment Record
Request Information Record
Message Terminator Record
Following each record is a list of the requirements and general considerations
regarding the contents of one or more fields of the record.
Message Header Record

Max.
Length
Access
Receive
Access
Send
Field
Repeat
Component
Escape
&
Description
Record Identifier
Delimiter
Example
Delimiters:
Message Control ID
Ignored
No
Access Password
Ignored
No
Sender Name or ID
40
Ignored
Yes
40
Ignored
Yes
System ID
LIS
* Required
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Message Header Record (continued)

Description
Delimiter
Example
Max.
Length
Access
Receive
Access
Send
Sender Street Address
Ignored
No
Reserved Field
Ignored
No
Sender Telephone Number
Ignored
No
Characteristics of Sender
Ignored
No
Receiver ID
Access
40
Yes
Yes
System1
40
Yes
Yes
Ignored
No
System ID
Comment or Special
Instructions
Processing ID
Yes
Yes
Version Number
12
Yes
Yes
Date and Time of Message
19971119161505
14
Yes
Yes
End of Record
<CR>
* Required
Processing ID
Indicates how this message is to be processed. The

following code is used:
P
Production: Treat message as an active

message to be completed according to
standard processing.
Sender Name
or ID
The Access System sends the word Access followed by a

System ID in a component field (for example, Access
System1). The System ID is entered in system
configuration in the Laboratory Information screen.
Receiver ID
Indicates the intended message receiver. If the Access

System will be the receiver, the word Access is used,
followed by an optional System ID. The System ID
should be designated only when there are multiple
Access Systems using the LIS, and the message is
intended only for the named Access System. The
message is rejected when Access is the Receiver ID and
the System ID component field does not match the
System ID entered and stored in the laboratory
information of system configuration.
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Patient Information Record

Description
Delimiter
Example
Max.
Length
Access
Receive
Access
Send
Record Identifier
Sequence Number
Practice Assigned Patient

ID
Smith, John
15
When
available*
Laboratory Assigned
Patient ID
Ignored
No
Patient ID #3
Ignored
No
Yes
No
Ignored
No
Patient Name:
30
Last Name
LASTNAME
First Name
FIRSTNAME
Middle Name
Suffix
JR
Title
DR
Mothers Maiden Name
Birthdate
19631124
Yes
No
Patient Sex
Yes
No
Patient Race
Ignored
No
Patient Address
Ignored
No
Reserved Field
Ignored
No
Patient Telephone
Ignored
No
Attending Physician ID
Ignored
No
Special Field 1
Ignored
No
Special Field 2
Ignored
No
Patient Height
Ignored
No
Patient Weight
Ignored
No
Patient Diagnosis
Ignored
No
Patient Medications
Ignored
No
Patient Diet
Ignored
No
* Required
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Patient Information Record (continued)

Description
Delimiter
Example
Max.
Length
Access
Receive
Access
Send
Practice Field #1
Ignored
No
Practice Field #2
Ignored
No
Admission and
Discharge Dates
Ignored
No
Admission Status
Ignored
No
Location
Ignored
No
Nature of Alt. Diag.

Code & Class.
Ignored
No
Alt. Diag. Code & Class.
Ignored
No
Patient Religion
Ignored
No
Marital Status
Ignored
No
Isolation Status
Ignored
No
Hospital Service
Ignored
No
Hospital Institution
Ignored
No
Dosage Category
Ignored
No
End of Record
<CR>
* Required
Practice Assigned
Patient ID
When this field is downloaded, it appears on the Test

Request screen in the Patient ID column. It is also sent
on uploads, if available. If the test order is entered at the
Access System and the operator does not enter a Patient
ID, this field is blank on uploads.
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11
Test Order Record

Description
Delimiter
Example
Max.
Length
Access
Receive
Access
Send
Record Identifier
Sequence Number
Specimen ID
SPEC1234
15
Ignored
No
Instrument Specimen ID:

Sample Tag
Tray Number
Ignored
Yes
Sample Position
10
Ignored
Yes
Universal Test ID:

Identifier
Ignored
No
Name
Ignored
No
Type
Ignored
No
Local Code: Test
Ferritin
Yes
Local Code: Replicate
Ignored
Yes
Priority
Yes
No
Requested Date and Time
Ignored
No
Collection Date and Time
Ignored
No
Collection End Time
Ignored
No
Collection Volume
Ignored
No
Collector ID
Ignored
No
Action Code
Yes
No
Danger Code
Ignored
No
Relevant Clinical Info.
Ignored
No
Date/Time Specimen Rcvd
Ignored
No
Yes
Yes
Ignored
No
Specimen Descriptor:
Specimen Type
Specimen Source
Serum
* Required
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Test Order Record (continued)

Description
Delimiter
Example
Max.
Length
Access
Receive
Access
Send
Ordering Physician
Ignored
No
Physicians Phone Number
Ignored
No
User Field #1
Ignored
No
User Field #2
Ignored
No
Laboratory Field #1
Ignored
No
Laboratory Field #2
Ignored
No
Date/Time Results
Reported/Mod.
Ignored
No
Inst. Charge to Computer

System
Ignored
No
Inst. Section ID
Ignored
No
Report Types
Ignored
Yes
Reserved Field
Ignored
No
Loc. Specimen Collection
Ignored
No
Noscomial Infection Flag
Ignored
No
Specimen Service
Ignored
No
Specimen Institution
Ignored
No
End of Record
<CR>
* Required
Specimen ID
Alphanumeric characters are accepted (0-9, A-Z both

upper and lower case, /, +, and -). Spaces and
punctuation are not allowed.
Instrument
Specimen ID
The three components are: Sample Tag, Tray Number,

and Sample Position. Tray Number and Sample Position
are uploaded to the LIS but are ignored on downloads.
The Tray Number is 1-9999, and the Sample Position is
the position within the sample tray (1-10). Sample Tag is
unused.
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Universal Test ID
The first three components of this field are not used by

the Access System, and should be left blank by the LIS
as they are ignored. The fourth component is required,
representing the test code, and should be the last
component specified. Refer to the Test Codes Used by
the Access System section later in this document.
On uploads, the Access System includes a fifth
component field indicating the test replicate number.
If one replicate of a test is ordered, the replicate number
is 1. If two replicates of a test are ordered, the test
appears in the test order record twice, with replicate
numbers of 1 and 2. Replicate numbers are ignored on
test order downloads.
The Access System parses repeat delimiters in this field
for received Test Order Records. The Access System may
also produce repeat delimiters in this field when
sending Test Order Records, to list each test code for
each Result Record to follow for a specific sample
position.
Repeat delimiters are not parsed or produced for this
field in the Result Record.
Priority
Test priority codes are as follows:

S
A
R
C
P
Stat
As Soon as Possible
Routine
Callback
Preoperative
Although the system accepts up to nine characters, the

priority code is the first character in the field.
Code S (Stat) is treated as a Stat patient sample by the
Access System. Any other priority code, including no
priority code, is treated as a routine patient sample by
the Access System.
Action Code
This field indicates what will be done with the

specimens accompanying or preceding this test order
request received by the Access System. The following
codes are used:
C
A
14
Cancel request for the test. The test is removed

from any test request and from the LIS pool.
Add the requested test to the existing
specimen with the patient and specimen
identifiers given in this record. If the specimen
does not exist, create it.
New requests accompanying a new specimen.
If the specimen already exists, the request is
considered invalid and is rejected.
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Specimen
Descriptor
Only the Specimen Type portion of the Specimen

Descriptor is used. The following types are supported
by the Access System: Serum, CSF, Urine, Blood,
Plasma, Other.
Report Types
The following codes are used by the Access System

when uploading only:
F
X
Final results.
Results cannot be done, request canceled.
Result Record
Description
Delimiter
Record Identifier
Sequence Number
Example
Max.
Length
Access
Receive
Access
Send
No
No
Universal Test ID:
No
Identifier
No
Name
No
Type
No
Local Code
Ferritin
Value
105.6
No
Yes
Interpretation
10
No
Yes
Data Measurement/ Value:
Units
ng/mL
No
Yes
Reference Ranges
23.9 to 336.2
21
No
Yes
Reference Type
Normal
No
Yes
Result Abnormal Flags
No
Yes
Nature of Abnormality
Testing
No
No
Result Status
No
Yes
Date of Change
No
No
Operator Identification
No
No
Date/Time Test Started
No
No
* Required
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15
Result Record (continued)

Delimiter
Example
Max.
Length
Access
Receive
Access
Send
Date/Time Test
Completed
19971119165845
14
No
Yes
Instrument
Identification
No
No
End of Record
<CR>
No
Description
* Required
Universal Test ID
For considerations regarding this field, see the Test

Order Record descriptions in this document.
Result Abnormal
Flags
This field indicates the status of the result. The

following codes are used:
L
H
LL
HH
>
N
<
Result Status
The following codes are used on result uploads:

F
X
16
Below normal ref. low limit. Indicates the

Access System ORL flag.
Above normal ref. high limit. Indicates the
Access System ORH flag.
Below panic low. Indicates the Access System
CRL flag.
Above panic high. Indicates the Access
System CRH flag.
Out of range high. Indicates the Access
System OVR flag.
Normal result. Result not flagged ORH, ORL,
CRL, CRH, or OVR.
Under for positive slope assays (or over for
negative slope assays) the threshold limit
defined in the assay protocol file. Indicates the
Access System UNR flag.
Final results.
Results cannot be done, request is not
honored.
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Comment Record
Description
Delimiter
Example
Max.
Length
Access
Receive
Access
Send
No
Record Identifier
Sequence Number
No
Comment Source
No
Yes
Comment Text
CEX; PEX
160
No
Yes
Comment Type
No
Yes
End of Record
<CR>
No
* Required
Comment Source
Used to indicate source of comment. The comment

record is only sent immediately following a result
record when the result contains one or more flags.
The following code is used:
I
Comment Text
Clinical Instrument System
Used to indicate result record flags. Not all flags apply

to all results. Some flags are currently not used, but are
listed here for future reference. The following codes are
defined:
CEX Curve expired.
CRH Result higher than critical (panic) reference
range.
CRL Result lower than critical (panic) reference
range.
EDT Result edit (currently unused).
GRY Qualitative result in gray zone.
IND Result is indeterminant.
LEX Lot expired.
LOW Result lower than the minimum reportable
result.
NCR No curve.
NRV Insufficient reagent, reaction vessels, wash,
substrate, waste, or reaction vessel waste.
OLD Sample old.
ORH Result higher than normal reference range.
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ORL Result lower than normal reference range.

OVR Result out of range of curve.
PEX Pack expired.
QCF Control is outside QC limits.
QEX Control lot expired.
QNS Quantity not sufficient.
RLU Relative light unit (RLU) value is outside of
the acceptable luminometer measuring range.
SYS
Instrument error.
TMP One or more of the following is outside the

temperature specification: Incubator, wash/
read carousel, substrate, reagent.
UNR The measured sample signal (RLU) is less than
an RLU threshold limit for the least
concentrated calibrator or calibration control
for a positive slope assay, or is greater than the
least concentrated calibrator for a negative
slope assay, as defined in the assay protocol
file.
WVL Quality control sample result was generated
using a different reagent vial lot number than
the vial lot number used to calibrate the assay.
Comment Type
Used to indicate type of comment. The following codes

are used:
G
Generic/Free Text Comment (for error

messages)
Instrument Flag Comment (for test result

flags)
Request Information Record

Description
Delimiter
Record Identifier
Sequence Number
Example
Max.
Length
Access
Receive
Access
Send
No
No
No
No
No
Yes
Starting Range ID Number:

Patient ID Number
Specimen ID Number
Samp45
15
*Required
18
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Request Information Record (continued)

Description
Delimiter
Example
Max.
Length
Access
Receive
Access
Send
Ending Range ID Number
No
No
Universal Test ID
No
No
Nature of Request Time

Limits
No
No
Beg. Request Results

Date/Time
No
No
Ending Request Results

Date/Time
No
No
Requesting Physician
Name
No
No
Requesting Physician
Telephone Number
No
No
User Field No. 1
No
No
User Field No. 2
No
No
Request Info. Status Codes
No
Yes
No
End of Record
<CR>
*Required
Starting Range ID
Number
Used by the Access System to query the LIS. This field

contains a single specimen ID which corresponds to the
Access Systems 15-character sample ID. The specimen
ID follows the unused patient ID in a component field.
Ending Range ID
Number
This field is unused, since the Access System does not

query for a range of specimen ID numbers.
NOTE
The Access System queries the LIS whenever a tray containing samples that have
no test requests is loaded. The following example displays how the Access
System queries the LIS for test information:
Tray 14 appears as follows at the time of the query:
Position
1
2
Sample ID
QN195823
KNH483736
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Tests Requested
Folate, Ferritin
TSH
19
Position
3
4
5
6
7
8
9
10
Sample ID
Samp45
F049498
456
SS51269
91292IGM
34543
D2SE3
JA456789
Tests Requested
TU
TotT4
TotT4, TU
Folate
TbhCG2
When tray 14 is loaded, the Access System identifies Samp45, 456, and
34543 as samples that require tests. The Access System queries the LIS for the
test information for each sample, starting with Samp45. The following is an
example of a query for the test information for Samp45:
H|\^&|||Access|||||LIS||P|1|19970901085833
Q|1|^Samp45||ALL||||||||O
L|1|F
After sending this message, the Access System waits for a response. The Access
System interprets the next message it receives as the response to its query. If the
LIS does not have any tests pending for Samp45, it should reply in a form
similar to the following:
H|\^&|
L|1|F
The Access System waits one minute for a response to the query. If the LIS does
not respond with a message in this time, the Access System assumes that no tests
are pending for Samp45. It then sends the LIS a query for 456. If the LIS
responds to the first query, the Access System immediately sends the next
pending query.
The Access System continues this cycle until it has issued a query for all sample
IDs that require test requests. If the host LIS sends a test request for a sample for
which the Access System has already prepared (but not yet sent) a query, the
Access System still sends the query.
20
3/00
Rev 101968P
Test Codes Used by the Access System
Message Terminator Record

Description
Delimiter
Example
Max.
Length
Access
Receive
Access
Send
Record Identifier
Sequence Number
Termination Code
Ignored
Yes
End of Record
<CR>
* Required
Termination Code
Provides explanation for end of session. The following

code is used:
F
Test Codes
Used by the
Access
System
Last request for information processed.
The following list contains typical test codes used by the Access System. This list
is subject to change without notification. Tests listed may or may not be available
for use with commercial systems. Contact your Beckman Coulter representative
for more information on test availability.
NOTES
You can view the list of available test codes on the Access System by
displaying the Configure Tests screen. Operators should provide the
vendor with a list of the test codes to be set up in the LIS.
All test codes are case-sensitive and must be entered as displayed on the
Configure Tests screen.
Access Test Codes

Access
Code
DL2000
Code
Assay Name
AbHBsII
A01
Hepatitis B Surface Antibody II (Not available in

the US)
AFP
A02
Alpha-Feto Protein (cancer and ONTD utility)
AgHBs
A64
Hepatitis B Surface Antigen (Not available in the

US)
8/05
Rev 101968X
21
Access Test Codes (continued)

Access
Code
22
DL2000
Code
Assay Name
AgHBsBlk
A65
Hepatitis B Surface Antigen Blocking (Not

available in the US)
AgHBsCtl
A66
Hepatitis B Surface Antigen Control (Not

BR15-3Ag
A04
BR Monitor
CEA
A06
Carcino-embryonic Antigen
Chl-Ag
A07
Chlamydia Antigen
ChlBlock
A08
Chlamydia Blocking
CK-MB
A49
Creatine Kinase - MB
Cortisol
A10
Cortisol
cTnI
A67
AccuTnI
DHEA-S
A73
Dehydroepiandrosterone Sulfate
Dig
A11
Digoxin
Dil-hCG2
A18
Diluted Human Chorionic Gonadotropin
ESTRDL
A14
Estradiol
FastTSH
A78
Thyroid Stimulating Hormone, Fast (2nd Gen)
Ferritin
A19
Ferritin
FOL2
A75
Folate (new formulation P/N A14208)
Folate
A20
Folate (previous formulation P/N 33010)
freePSA
A63
Free Prostate Specific Antigen
FreeT3
A21
Free Triiodothyronine (previous formulation

P/N 33850)
FT3
A80
Free Triiodothyronine (new formulation P/N A13422)
FT4
A61
Free Thyroxine
FTIndex
A24
Free Thyroxine Index (Calculated using TotT4

and TU results; do not order this test)
GI19-9Ag
A05
GI Monitor
HAVAb
A26
Hepatitis A Virus Total Antibody (Not available

in the US)
HAV-IgM
A25
Hepatitis A Virus Immunoglobulin M (Not

8/05
Rev 101968X

Access
Code
DL2000
Code
Assay Name
HAV-IgM
A25
Hepatitis A Virus Immunoglobulin M (Not

HBc-Ab
A27
Hepatitis B core Total Antibody (Not available in

the US)
HBc-IgM
A28
Hepatitis B core Immunoglobulin M (Not

HBsAg
A31
Hepatitis B Surface Antigen (previous

formulation)
HBsAgBlk
A32
Hepatitis B Surface Antigen Blocking (previous

formulation)
HBsAgCtl
A33
Hepatitis B Surface Antigen Control (previous

formulation)
HCVPLUS
A34
Hepatitis C Virus Antibody Plus (Not available in

the US)
hFSH
A36
Human Follicle Stimulating Hormone
hGH
A22
Human Growth Hormone
HIVAbNew
A37
HIV 1, 2 New (Not available in the US)
hLH
A39
Human Luteinizing Hormone
IFAb
A72
Intrinsic Factor Antibody
IgG-Toxo
A53
Toxoplasmosis Immunoglobulin G
IgM-Toxo
A13
Toxoplasmosis Immunoglobulin M
Insulin
A40
Insulin
MYO
A30
Myoglobin
Ostase
A12
Ostase
OV125Ag
A03
OV Monitor (CA 125 Antigen)
PRL
A42
Prolactin
Prog
A43
Progesterone
PSA-Hyb
A62
Hybritech Prostate Specific Antigen
RBC2
A76
Red Blood Cell Folate (new formulation P/N A14208)
RBCFol
A45
Red Blood Cell Folate (previous formulation P/N

33010)
8/05
Rev 101968X
23

Access
Code
24
DL2000
Code
Assay Name
Rub-IgG
A46
Rubella Immunoglobulin G
Rub-IgM
A47
Rubella Immunoglobulin M (Not available in the

US)
SpIgE
A58
Specific Immunoglobulin E
TBhCG2
A17
Total Beta Human Chorionic Gonadotropin
Testo
A16
Testosterone
Tg
A23
Thyroglobulin
TgAb
A68
Thyroglobulin Antibody
Theo
A48
Theophylline
TotIgE
A51
Total Immunoglobulin E
TotT3
A50
Total Triiodothyronine
TotT4
A52
Total Thyroxine
TSH
A57
HYPERsensitive Thyroid Stimulating Hormone

(3rd generation)
TU
A59
Thyroid Uptake
uE3
A15
Unconjugated Estriol
VitB12
A60
Vitamin B12
8/05
Rev 101968X
Sample Messages
Upload Example 2
The Access System reports results for Sample ID 47G. One replicate of Folate,
Ferritin, VitB12, and Rub-IgG were requested. The test request was entered at the
keyboard, without a Patient ID. The Rub-IgG test did not obtain a printable result.
+_?A ___$FFHVV_____/,6__3__
3__
2__*_AA_AAA)RODWHA?AAA)HUULWLQA?AAA9LW%A?AAA5XE,J*A___________6HUXP__________)
5__AAA)RODWHA__QJP/__1__)____
5__AAA)HUULWLQA__QJP/__1__)____
5__AAA9LW%A__SJP/__1__)____
5__AAA5XE,J*A_1R9DOXH_5/8__1__)____
&__,_5/83(;_,
/__)
Upload Example 3
The Access System operator cancels the TSH and Theo tests for Sample ID W4,
Patient ID 346;SIS.
+_?A ___$FFHVV_____/,6__3__
3__6,6
2__:_AA_AAA76+A?AAA7KHRA___________6HUXP__________)
5__AAA76+A______;
5__AAA7KHRA______;
/__)
Upload Example 4
The Access System reports results for Sample ID 002. Two replicates of Chl-Ag
and two replicates of ChlBlock were requested.
+_?A ___$FFHVV_____/,6__3__
3__$GDPV-RKQ
2___AA_AAA&KO$JA?AAA&KO$JA?AAA&KO%ORFNA?AAA&KO%ORFNA___________6HUXP__________)
5__AAA&KO$JA_A1RQ5HDFW_6&2__1__)____
&__,_&(;_,
5__AAA&KO$JA_A5HDFWLYH_6&2__1__)____
&__,_&(;_,
5__AAA&KO%ORFNA_A1RW&RQI_6XSS__1__)____
&__,_&(;_,
5__AAA&KO%ORFNA_A&RQILUPHG_6XSS__1__)____
&__,_&(;_,
/__)
3/00
Rev 101968P
25
Sample Messages
Upload Example 5
The Access System reports results for Sample ID 661. Two replicates of TotT4 and
two replicates of TU were requested. The FTIndex test was not explicitly ordered,
but is generated automatically when valid TotT4 and TU results are obtained for a
sample, regardless of the setting of the FTI Ratio option in system configuration.
+_?A ___$FFHVV_____/,6__3__
3__'2(MDQH
2___AA_AAA7RW7A?AAA7RW7A?AAA78A?AAA78A?AAA)7,QGH[A__________6HUXP__________)
5__AAA7RW7A__XJG/__1__)____
5__AAA7RW7A__XJG/__1__)____
5__AAA78A____1__)____
5__AAA78A____1__)____
5__AAA)7,QGH[A__XJG/__1__)____
/__)
Upload Example 6
The Access System sends rejection notification messages for Sample IDs W3 and
W4. These Sample IDs already exist in the Access System LIS pool and therefore
cannot be accepted as new test orders (action code N in the test order download
record).
+_?A ___$FFHVV_____/,6__3__
3__'5&
2__:__AAA7KHR___________6HUXP__________;
&__,_1HZRUGHULQYDOLGVSHFLPHQDOUHDG\H[LVWV_*
3__'5&
2__:__AAA76+___________6HUXP__________;
3__,'*
2__:__AAA7KHR___________6HUXP__________;
3__,'*
2__:__AAA76+___________6HUXP__________;
/__)
26
3/00
Rev 101968P
Sample Messages
Request Information (Query) Example

The Access System sends a query to the LIS for test information for sample ID
Samp45, after the tray containing this sample is loaded onto the analyzer.
+_?A ___$FFHVV_____/,6__3__
4__A6DPS__$//________2
/__)
Query Response Test Available

The LIS responds to a query for test information for sample Samp45 with a
message indicating the TSH test.
+_?A ___+RVW/,6_____$FFHVV__3__
3__$EHO&LQG\
2__6DPS__AAA76+_5______$____6HUXP
/__)
Query Response Test Unavailable

The LIS responds to a query for test information for sample Samp45 with a
message indicating the no tests are available for the sample.
+_?A _
/__)
3/00
Rev 101968P
27
Limits and Performance Guidelines
Limits and
Performance
Guidelines
The following table lists the limits for messages downloaded from the LIS to the
Access System. To ensure good system performance, use the Good Performance
Limits or Optimal Performance Guidelines rather than the Absolute Limits.
The Absolute Limit column lists system maximums. Messages
exceeding any of these limits are not processed completely and/or
properly. DO NOT violate these limits.
The Good Performance Limit column lists limitations that result in good
system performance. These limits are generally easily met in clinical
environments.
The Optimal Performance Guidelines column lists limitations that
afford the Access System the best performance with the LIS.
A description of each of the specifications, and an example of an optimal
download message follow the table.
Specification
Absolute Limit
Good Performance
Limit
Optimal Performance
Guidelines
Max. Tests per Sample
99
40
10
Max. Data Characters per

Frame
240
240
240
Max. Total Characters per

Frame
247
247
247
Max. Record Length
1024 characters
1024 characters
240 characters
Max. Message Size
102,400 characters
122 frames
5 frames
Max. Samples in Database
1000
N/A
N/A
Max. Tests in Database
2200
N/A
N/A
28
Max. Tests per

Sample
The maximum number of tests that can be ordered on a

particular sample is 99.
Max. Data
Characters per
Frame
The maximum number of characters that can be

contained in the [DATA] portion of the frame. This
number is determined by ASTM Standard E1381-95.
Records containing more than 240 characters must be
spread over multiple frames.
Max. Total
Characters per
Frame
The maximum total number of characters that can be

contained in a frame, as determined by ASTM Standard
E1381-95. This number includes the 240 data characters
plus the 7 characters associated with sending a frame
(i.e., <ETX>).
3/00
Rev 101968P
Max. Record
Length
The maximum number of data characters that can be

contained in one record. A 1024 character record
requires 5 frames: 4 of 240 characters each, and 1
containing 64 characters.
Max. Message
Size
The maximum sum total of all the data characters in a

message. Since frames vary in length, the exact number
of frames that the maximum number of characters
requires cannot be determined, but messages
approaching the maximum are highly discouraged.
Max. Samples in
Database
The Access System allows a maximum of 1000 sample

records in the sample database. A sample record is
created in the database for each Order record received.
Sample records are cycled through the database on a
first-in-first-out basis, with unloaded sample records
being removed before those that have not yet been
loaded.
Max. Tests in
Database
The Access System allows a maximum of 2200 test

records in the sample database. A test record is created
in the database for each test ordered on a sample.
Sample records are cycled through the database on a
first-in-first-out basis, with unloaded sample records
being removed before those that have not yet been
loaded.
Optimal Download Example

+_?A ___+RVW/,6_____$FFHVVA6\VWHP__3__
3_____%DNHUA7RPA0A6A0U___0
2____AAA76+?AAA76+_5______$____6HUXP
2____AAA&RUWLVRO_5______$____6HUXP
/__)
3/00
Rev 101968P
29
Printed in U.S.A.
30
3/00
Rev 101968P

Access Interfaceamento

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Immunoassay System

LIS Vendor Information

Ostase is trademark of Hybritech Incorporated, a subsidiary of Beckman Coulter, Inc.

Frame Layer Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Record Field Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Test Codes Used by the Access System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Limits and Performance Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

2000 Copyright Beckman Coulter, Inc.

Access LIS Vendor Information

2000 Copyright Beckman Coulter, Inc.

Access LIS Vendor Information

LIS Vendor Information

LIS Vendor Information

2000 Copyright Beckman Coulter, Inc.

Access LIS Vendor Information

Acknowledgment (ASCII Decimal 6).

The most significant character of Checksum.

The least significant character of Checksum.

The data contents of the record.

Inquire (ASCII Decimal 5).

End of Transmission Block (ASCII Decimal 23). For use

End of Text (ASCII Decimal 3). Required at the end of

Single digit frame number 0 to 7. Starts with 1.

Line Feed (ASCII Decimal 10).

Negative Acknowledgment (ASCII Decimal 21).

Start of Frame (ASCII Decimal 2).

All framing required for transmission of data. This

One of several related pieces of information within a field.

The transmission of data from the LIS to the Access

A specific location within a record for a piece of

A complete communications packet.

Laboratory Information System.

A collection of related information; a group of records

The device that responds to the sender. The receiver in

2000 Copyright Beckman Coulter, Inc.

Access LIS Vendor Information

End of Transmission (ASCII decimal 4).

Carriage Return (ASCII decimal 13).

In reference to the low level protocol, a record is the

An additional field of the preceding type when indicated

The device that has a message to send and initiates the

A total unit of communication activity used in this

A determination of a single analyte or a combination of

The transmission of data from the Access System to the

2000 Copyright Beckman Coulter, Inc.

Access LIS Vendor Information

Frame Layer Protocol

Receiver Not Ready

Transport Layer Sequence

2000 Copyright Beckman Coulter, Inc.

Access LIS Vendor Information

Frame Layer Protocol

Failure Session (NAK):

Failure Session (Max <NAK>s):

Failure Session (No Response):

(Time-out after 15 seconds)

Failure Session (Max <NAK>s):

<STX> [F1] [DATA] <ETX> [C1] [C2] <CR> <LF> ->

<STX> [F1] [DATA] <ETX> [C1] [C2] <CR> <LF> ->

2000 Copyright Beckman Coulter, Inc.

Access LIS Vendor Information

Failure Session (Max <NAK>s):

<STX> [F1] [DATA] <ETX> [C1] [C2] <CR> <LF> ->

All communications are expected to use the RS232 communication protocol,