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International Journal of Pharmacy and Pharmaceutical Science Research

Universal Research Publications. All rights reserved

ISSN: 2249-0337
Original Article
PHYTOCHEMICAL ANALYSIS OF LEAVES OF TRIGONELLA FOENUMGRAECUM L.FROM ANJANGAON, AMRAVATI REGION OF
MAHARASHTRA STATE
R.S.SHAIKH, F.S. SYED, D.T.TAYADE
Department of chemistry, Government Vidharbha Institute of Science and Humanities,
Amravati (M.S). PIN -444604, INDIA.
Received 06 June 2013; accepted 28 June 2013
Abstract
India is reach store house of medicinal plants.All natural product can be termed bioactive molecules, as every diverse
molecules possesses one kind or multiple kinds of biological oblique pharmacological activities. These small molecules
provide the source or inspiration for the majority of FDA-approved agents and continue to be one of the major sources of
inspiration for drug discovery. In particular, these compounds are important in the treatment of life-threatening
conditions.[1] . The beauty about natural products (Herbal Drugs) libraries is the uniqueness in there chemical structural
diversity and innovativeness that in compasses varied biological actions .Contribution of plants is remarkable in diversified
industries such as drugs and pharmaceuticals, fine chemicals, cosmetics, industrial raw materials etc. Some of the drugs
used presently are obtained from the different plant sources. The latest investigations show that 60% of the anticancer and
anti infective drugs are commercially obtained from the plant sources.Medicinal plants are finding a new, expanding
market as herbal components of health foods and preventative medicines, especially under the marketing term
"nutraceuticals. In the past, traditional peoples or ancient civilizations depended greatly on local flora and fauna for their
survival[2].Today India is a formidable force in generic world pharmaceutical market in the last 20 years ,Indian chemist
have made great contribution in developing cutting edge technology of active pharmaceutical ingredients .Hence taking all
this thinks into consideration it create sufficient interest to carry out phytochemical analysis of leaves of TRIGONELLA
FOENUM GRAECUM L. from ANJANGAON region in Amravati district of Maharashtra state of India, due to the
important pharmaceutical and medicinal properties of TRIGONELA FOENUMGRACUM.
2013 Universal Research Publications. All rights reserved
Keyword: - ANJANGAON,TRIGONELLA FOENUM GRAECUM L. Phytochemical analysis .
Introduction :
ANJANGAON is located in Amravati district in
Maharashtra state of India. This area is very rich of flora
and fauna having various type medicinal plants as it is
present in the ranges of Satpuda mountain. Trigonella
foenum graecum L., vernacular name fenugreek, is
anannual herb of Leguminosae. which is used as spice and
forage .It is cultivated in India, Pakistan and in the
Mediterranean countries .and considered as a medicinal
plant .which is known to have several pharmacological
effects
like
hypoglycaemic,
hypercholesterolemic,
antioxidative, laxative and fungicide effects and appetite
stimulation. Seeds are rich in magnesium, sodium and
phosphorus (3-9). Nowadays, fenugreek is widely cultivated
as a drug plant. The mucilaginous seeds are reputed to have
many medicinal virtues, as a tonic, emollient,
carminative,demulcent, diuretic, astringent emmenagogue
,expectorant, restorative, aphrodisiac and vermifugal
properties and were used to curemouth ulcers, chapped lips

94

and stomach irritation . In Iranian traditional medicine the

seeds are used as tonic and blood sugar lowering (10-11).
Fenugreek leaves and seeds have been used extensively to
prepare extracts and powders for medicinal uses. Fenugreek
is reported to have anti-diabetic, anti-fertility, anticancer,
anti-microbial, anti-parasitic and hypocholesterolaemic,
effects. In India, fenugreek is used as a lactation stimulant.
Fenugreek seed in powder or germinated form exhibits
anti-diabetic properties, hypocholesterolaemic effect, anticancer effect, effect onthyroxine-induced hyperglycaemia
and protective effect on ethanol toxicity (12-20).Fenugreek
has been referred to as a medicinal herb both in Indian
Ayurvedic and traditional Chinese medicines. . Medicinal
uses vary from wound-healing to bust enhancement and
from promotion of lactation in weaning mothers, to its use
as a sex stimulant or aphrodisiac. Fenugreek plant extract
was successful in complete removal of head lice from
infected patients within a week. .Recently in this laboratory
enormous work on natural products was carried out by one

International Journal of Pharmacy and Pharmaceutical Science Research 2013; 3(3): 94-96

researches group of R.S.SHAIKH (21-28) especially

on proximate, phytochemical, physiochemical and
spectroscopical analysis of various plants and as a part of
research work, presently being undertaken in this
laboratory on natural products, hence it appeared sufficient
interesting to carry out phytochemical analysis of the leaves
of Trigonella Foenum Gracum L.
hytochemistry is branch of botany in which there is inter
disciplinary linkage with chemistry. In phytochemistry the
elemental analysis and individual organic compounds
present in the plants were investigated by conventional
chemical and chromatographic techniques. The literature
review reveals that now a days botanist are much more
interested to find out elements and organic compounds in
different parts of plants but chemists and researchers in
chemistry are not much more aware and interested in
phytochemistry or phytochemical analysis of leaves of
TRIGONELLA FOENUM-GRAECUML . by considering
this facts and the medicinal values of this plants
phytochemical analysis of leaves of TRIGONELLA
FOENUM-GRAECUM L.from ANJANGAON region was
carried out.
Preparation sample:
First the site for leaves collection in ANJANGAON was
decided. A farm of Mr. RATHOD. The total leaves were
collected from same region. The collection of sample was
done in between 11th February 2013 to 16th February
2013.The site of collection belong to ANJANGAON,
which situated in latitude 210 09 52 and longitude 77 0
1846.The plant OF TRIGONELLUA FOENUM
GRAECUM L. was carefully collected The leaves of
TRIGONELLUM FOENUA GRAECUM L. were separated
by scissor and air dried under shade . They were dried at
room condition .The leaves were pulverized in grinding
mill being a screen of 5mm diameter hole to achieve
particle size 40-60 mesh. This fine powder were treated as
sample and stored in an airtight container for further use.
All chemicals used are of A.R. grade.
Sr.No.
Element/Compound
Results
1
Magnesium
Absent
2
Calcium
Absent
3
Sulphur
Present
4
Iron
Present
5
Sodium
Present
6
Chlorine
Present
7
Glucosides
Present
8
Acubine type of Glucosides
Present
9
Cyanogenic Glucosides
Present
10
Phenol
Present
11
Flavanol
Present
S
Amino acids
Present
13
Syringic acid
Absent
14
Alkolides
Present
15
Steroides
Present
16
Tannin
Present
17
Polysaccarides
Present
18
Pectin
Present
19
Hemicellilous
Present
20
Fats
Present
21
Volatile oil
Present

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Determination of Mg, Ca, S, and Fe element:

Leaves sample was taken and kept in furnace at 400 0C
for12 hours. Then there is formation of ash. The ash was
transferred into conical flask to it 20% hydrochloric acid
was added, the reaction mixture was continuously shaken
vigorously for 1 hour and it was filtered. The filtrate was
taken to determine Mg, Ca, S and Fe elements. Qualitative
analysis was carried out by given literature method18 and
results is given in Table No.1
Determination of Glucosides, Cynogenic glucosides,
Acubin type of glucosides, Phenol, flavonols, amino acid,
Syringic acid, Alkaloides, steroids, Tannin, polysaccharide,
Pectin and Hemicelluloses, fats volatile oil was carried out
by known literature method(29) .
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Source of support: Nil; Conflict of interest: None declared

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International Journal of Pharmacy and Pharmaceutical Science Research 2013; 3(3): 94-96