Beruflich Dokumente
Kultur Dokumente
1. What are Protoplasts? Discuss different methods for isolation & purification of
Protoplast. [16]
2. Write a short note on permeabilization of cells. Explain how it can be used for
secondary metabolites production? List out some of the advantages. [16]
3. Discuss the various strategies involved in the production of rare fatty acids in plants.
Explain with examples. [16]
(a) Turbine
(b) Chemostat
(c) Turbidostat
(d) Rheology [4+4+4+4]
6. What are haploids?Explain how anther and pollen cultures are useful for the pro-
duction of Haploids? [16]
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1
Code No: 45056 R07 Set No - 2
III B.Tech I Semester Regular Examinations,Nov/Dec 2009
PLANT BIOTECHNOLOGY
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????
6. Write about Somoclonal variation in detail Discuss the merits and demerits of
Somoclonal variation. [16]
7. Discuss the techniques of selecting cell lines for high yields of secondary compounds.
[16]
8. What are various methods used to measure growth of cells in cell suspension
cultures? [16]
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2
Code No: 45056 R07 Set No - 3
III B.Tech I Semester Regular Examinations,Nov/Dec 2009
PLANT BIOTECHNOLOGY
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????
1. Explain
4. Explain the various strategies for insect resistance by Bacillus thuringienesis. [16]
5. Write short notes on molecular farming of proteins? What are different products
produced by this method and the economic area. [16]
7. Write notes on
8. Enumerate how the Agrobacterium mediated gene transfers are different from other
viral vectors mediated gene transfer. [16]
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3
Code No: 45056 R07 Set No - 4
III B.Tech I Semester Regular Examinations,Nov/Dec 2009
PLANT BIOTECHNOLOGY
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????
2. Explain the different abiotic stresses involved in creating a water deficit. [16]
(a) Glyphosate
(b) Phosphinothricin
(c) PPT
(d) Atrazine [4x4]
4. Explain with neat diagram how molecule is synthesized by transgenic plants. [16]
8. “Plant tissue culture can act as alternative for the production of secondary metabo-
lites” justify the statement with suitable examples. [16]
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