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Genetic Control:
DNA and RNA
Structure of DNA
and RNA
Nucleotides
DNA replication
Polynucleotides
DNA, RNA
Protein
Synthesis
Nucleic Acids
Phosphate
Phosphate
Sugar
Sugar
Pentose sugar
5-carbon pentose sugar.
Nucleotides have 2 kinds of pentose; units
of RNA contains ribose sugar and DNA
contains deoxyribose sugar
The base of a nucleotide is covalently
joined to C1 of the pentose through
glycosidic bond (N-1 of pyrimidine ; N-9 of
purine)
The phosphate of a nucleotide is covalently
joined to C5 (-OH) of the pentose through
phosphoester bond.
Nitrogenous base
Large, double ring molecule found in both
DNA and RNA.
Adenine (A) and Guanine (G)
N-9 of the ring will form covalent bond
(glycosidic bond) with C1 of the pentose in a
nucleotide.
Phosphate
Purine base
Phosphate
Sugar
Sugar
There are two types of bases - purine and pyrimidine you only really need to know that adenine and guanine
are the purine bases and that purine bases are larger.
Nitrogenous Base
Purine
Adenine
Pyrimidine
Thymine (DNA)
Guanine
Cytosine
Uracil (RNA)
Polynucleotide
To form both DNA
and RNA, many of
these nucleotides
are linked together
in a polynucleotide
chain.
The structure of this
poly nucleotide
chain is seen in this
picture
Polynucleotide
Nucleotides are
connected to each
other via the
phosphate on one
nucleotide (C5) and the
sugar (C3)on the next
nucleotide
A Polynucleotide
Sugar-phosphate bonds
(backbone of DNA)
A-T(U) and G-C
Phosphodiester
bond
Hydroxyl grp
covalently attached
to C3 of the pentose
sugar, hence -3 end
term is used.
DNA molecules
are simply two of
these strands next
to each other,
running in
opposite
directions held
together by
hydrogen bonds.
31.0
27.3
25.6
23.0
24.6
31.5
27.6
25.3
23.3
24.3
19.1
22.5
24.5
27.1
25.5
18.4
22.5
24.6
26.6
25.6
Polynucleotides
DNA molecules
forms a 3D double
helix
shape, bonded by
hydrogen,
whereas RNA
remains as single
strands of
polynucleotide.
The Rule:
Adenine always base pairs with Thymine (or
Uracil if RNA)
Cytosine always base pairs with Guanine.
This is because there is exactly enough room
for one purine and one pyramide/pyrimidine
base between the two polynucleotide strands
of DNA.
Types of RNA
There are three major classes of RNA. Each
class of RNA has its own unique size, shape
and function in protein synthesis
Ribosomal RNA (rRNA) alone with ribosomal
proteins, makes up the ribosomes, where proteins
are synthesised.
Messenger RNA (mRNA) takes a message from DNA
in the nucleus to the ribosomes in the cytoplasm
Transfer RNA (tRNA) transfers amino acid to the
ribosomes
RNA vs DNA
RNA is a nucleic acid like DNA, but with 4
differences:
RNA has the sugar ribose instead of deoxyribose
RNA has the base uracil instead of thymine
RNA is usually single stranded
RNA is usually shorter than DNA
Dianne Chabira
DNA Replication
dispersive
model
dispersive
model
dispersive
model
dispersive
model
Experimental proof
Nitrogen is a major constituent of DNA, and
there are two different isotopes - 14N and
15N.
14N is by far the most abundant isotope of
nitrogen, but DNA with the heavier 15N
isotope also works.
1.
2.
3.
4.
5.
6.
7.
8.
Replication fork
Helicase
Continuous synthesis
DNA polymerase III
Discontinuous synthesis
RNA primer
DNA polymerase I
DNA ligase
The Codon???
A triplet of bases that codes for a specific
amino acid is called a codon.
Hence, the complementary set of triplet bases
for the codon is knows as the anticodon.
Short piece
of DNA
carries the
instruction
to the cell.
Deciphering
the codes
of life!!
mRNA
RNA in protein
synthesis
rRNA ribosome
tRNA
Protein Synthesis
There is a direct relationship between the
base sequence of DNA and the sequence of
amino acids that makes up a protein
DNA
mRNA
tRNA
protein
triplet
codon
anticodon
Amino acid
CCA
GGU
CCA
Glycine
AAA
UUU
AAA
phenylalanine
GTG
CAC
GUG
histidine
Transcription
Transcribe means to
copy using DNA as a
template,
complementary RNA
nucleotides are joined
to make mRNA
Transcription
RNA polymerase
unwinds a short
section of the DNA
double helix near
the start of the gene.
This unwound
section is known as
the transcription
bubble.
Transcription
The RNA polymerase,
and therefore the
transcription bubble,
travels along the
coding strand in the 5'
to 3' direction, and
along the noncoding
strand in the opposite,
3' to 5', direction,
Transcription
as well as polymerizing a
newly synthesized strand
in 5' to 3' or downstream
direction. The DNA
double helix is rewound
by RNA polymerase at
the rear of the
transcription bubble.
Like how a zipper works,
only it unzips it and rezips
it without going back and
forth.
Transcription
Where the helix is unwound, the
coding strand consists of
unpaired bases, whilst the
template strand consists of an
RNA:DNA composite, followed
by a number of unpaired bases
at the rear.
This hybrid consists of the mostrecently-added nucleotides of
the RNA transcript,
complementary base-paired to
the template strand
Transcription
mRNA can now leave
the nucleus.
Each triplet code of
bases from DNA is now
represented by three
bases on mRNA called
a codon.
Stages of Translation
Structure of tRNA
Anatomy of a ribosome
Initiation of translation
Sequence in mRNA
is complementary
to a sequence in
the tRNA anticodon
in the small
ribosomal subunit
Translation elongation
Termination of translation