Beruflich Dokumente
Kultur Dokumente
Driving Questions
In this lab you will compare the respiration rates in dormant and germinating seeds.
Do both germinating and non-germinating beans undergo cellular respiration?
What is the effect of temperature on respiration rates in beans?
If Crickets are used What is the effect of temperature on respiration rates in crickets.
Background
The term respiration refers to the exchange of gases between and organism and its environment.
This intake of oxygen gas and exhalation of carbon dioxide gas is closely linked to the production of
ATP at the cellular level, a process called cellular respiration. ATP is generated by mitochondria
within the cell. During cellular respiration, the energy stored within macromolecules such as
glucose is released and harnessed to phosphorylate ADP, producing ATP. In the presence of oxygen,
glucose can be fully oxidized releasing large amounts of energy.
Ice
1 temperature probe
Glass beads
Lab Safety
Emphasize the need to keep water away from any non-waterproof electronics.
Keep water away from electrical outlets.
Sequencing Challenge
The steps below are part of the Procedure for this lab activity. They are not in the right order. Determine the
proper order and write numbers in the circles that put the steps in the correct sequence.
Determine the
amount of carbon
dioxide produced
by dry peas at
room temp.
Determine the
amount of carbon
dioxide produced
by germinating
peas at 10 C.
Determine the
amount of carbon
dioxide produced
by germinating
peas at room
temperature.
Determine the
amount of carbon
dioxide produced
by germinating
peas that have
been boiled (or
incubated at
some other
temperature.
___________________________________________________________________________________________
___________________________________________________________________________________________
10. Obtain the plastic bottle containing the 50 germinating beans that have been incubating in
on ice water bath, blot dry and place into the plastic bottle. Re-insert the CO 2 gas sensor into
the bottle.
11.
12.
After completing step 11, run a similar experiment for germinating peas at a temperature
besides 10C (on ice) or room temperature.
Data Analysis
16.
Determine the rate of CO2 production by the beans and crickets in each run. Record all
appropriate data in table 5.2 (below)
Table 5.2: Rates of cellular respiration for test subjects at varying temperatures.
Avg. Temp
Run
Minimum
Maximum
Respiration
Set-Up
During
#
[CO2] ppm
[CO2] ppm
Rate ppm/sec
run
1
Dry Peas
Germinating Peas
(room temp)
Germinating Peas
(chilled)
Germinating Peas
(alternate temp)
17.
Using a graphing template (See last page), prepare a graph of respiration rates for all peas in
all runs. Be sure label your axes with units, and set an appropriate scale. Include a legend
with your graph indicating the test subjects and the temperatures of each run.
___________________________________________________________________________________________
__________________________________________________________________________________________
__________________________________________________________________________________________
___________________________________________________________________________________________
2. Does temperature affect the rate of cellular respiration in crickets? Further, does
temperature affect the rate of cellular respiration in crickets? If so, what evidence do you
have to support your claims? ____________________________________________________________
__________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
__________________________________________________________________________________________
___________________________________________________________________________________________
3. Explain differences in the respiration rates of room temperature and refrigerator
temperature crickets. Make sure to include the role of enzymes in these metabolic
pathways as well as transport across cell membranes in your answer.
__________________________________________________________________________________________
___________________________________________________________________________________________
__________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
4. Explain why carbon dioxide production is a valid way to determine rates of cellular
respiration. ______________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
__________________________________________________________________________________________
5. Why did we use CO2 production instead of oxygen consumption as a metric of cellular
respiration?
___________________________________________________________________________________________
__________________________________________________________________________________________
__________________________________________________________________________________________
Synthesis Questions
Use available resources to help you answer the following questions.
1. _Imagine that we conducted the room temperature experiment with the same numbers
of different beans (Alaska peas, chick peas, lima beans). Would you anticipate that all
seeds would show similar rates of cellular respiration? Why or why not________________
___________________________________________________________________________________________
___________________________________________________________________________________________
__________________________________________________________________________________________
___________________________________________________________________________________________
_________________________________________________________________________________________
2. If you used a similar experimental design to test the effects of 10 C on a 25-g mammal,
and a 25-g reptile what results would you expect? Explain your reasoning. ___________
___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
__________________________________________________________________________________________
3. Now, take the same small mammal you stuffed in the experimental chamber during
synthesis question 2 and subject the little varmint to a temperature change of 21C to
4 C. What would happen to the CO2 concentration in the experimental chamber? Explain
your reasoning.
___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
_________________________________________________________________________________________
__________________________________________________________________________________________
4.
In the space below graph the rates of cellular respiration of beans that began germinating at
the following points in the past (12 hours ago, 24 hours ago, 48 hours ago, and 72 yours ago).
Offer a brief explanation for your predicted results.
______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
AP Biology Graphing Template
Fall 2010
AP lab 5
Title:_______________________________________________________________________