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Biology

Experiment
Photosythesis Process

Timothy Abdiel_8A@23

Test for Amylum / Starch


Aim
: To show the presence of starch in a leaf that has
photosynthesized
Skills
:
You will learn to :
- Investigate the presence of starch in leaves
- Test for starch in leaf using iodine solution
Apparatus & Materials :
1. A test tube
2. A beaker glass
3. A wire gauze
4. Alcohol
5. A tripod stand
6. A glass rod
7. A bunsen burner
8. Iodine solution
9. A pair of forceps
10. A petri disk
11. A small, fleshy plucked leaf which has been exposed to sunlight for a few
hours
Procedures :
Glucose is produced when green plant photosynthesis. Excess glucose is
convered into starch. One of the way to demonstrate that the plant has
photosynthesis is to test the laves for starch using iodine test
1. Heat half a beaker of water over the bunsen burner until the waer boils
2. Turn of the bunsen burner
3. Put the leaf into the hot water
- Why is this step necessary?
Non-activated the cells inside the leaf and made the leaf permeable to
iodine solution
4. Put the boiled leaf in a test tube/boiling tube containing some alcohol. Then,
place the test tube/boiling tube in the beaker of hot water
Never heat alcohol over bunsen burner
a. What was the color of the alcohol before the leaf was put into the beaker ?
Transparent
b. What was the color of the alcohol 10 minutes after the leaf was put into
the beaker?
Reddish
c. What was the alcohol done to the leaf?
To dissolve the chlorophyll

5. The leaf is now very brittle. Gently remove the leaf with a pair of forceps. Put
it back into hot water. This softlens the leaf and makes it mmore permeable
to iodine
6. Remove the leaf and place it in a Petri disk. Add a few drops of iodine
solution until the leaf is just covered.
Explain what you observe after a few minutes!
The part of leaf that not covered with allumunium foil become dark blue, but
the part of leaf that covered with allumunium foil become pale
Conclusion :
The part of leaf that covered with allumunium foil become pale because it cant
absorb the sunlight energy and cant do photosynthesis, so it cant make amylum.

Test for Oxygen


Aim
: To show the presence of oxygen from green plant that has
photosynthesized
Skills
:
You will learn to :
- Investigate the presence of oxygen from green plant
- Test for oxygen from green plant using match stick
Apparatus & Materials :
1.
2.
3.
4.
5.
6.

A beaker glass
A funnel glass
A boiling tube
Safety match
Hydrilla sp.
Water

Procedure

1. Put Hydrilla sp. Plant into a beaker glass that is filled by water, then cover it
with funnel glass and boiling tube
2. Put the apparatus on the place which gets enough sunlight
3. Pay attention to emerge Hydrilla sp. Plant after getting sunlight
4. After the gas in tube much enough, lift the boiling tube and cover the nozzle
of the tube with your thumb
5. Open the tube by lifting the thumb and soon put burning match closer to
nozzle of the boiling tube
6. Observe what happened to the burning match
Question

1. Where does bubble air in the tube come from?


From the branch of Hydrilla sp.
2. Why must the apparatus be on the place that get enough sunlight?
Because, if theres enough sunlight, the changes of water become oxygen
will faster
3. What happened if the apparatus is placed on the dark place during the
experiment?
Theres no photolysis of water become oxygen.
Conclusion

The fire on the match become bigger, because theres oxygen that made from
the photolysis of water (light reaction)