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Genetic material is first subject to restriction enzymes which cuts the DNA sequence in
accordance to their recognition sequences [restriction enzymes may be also used to introduce a
DNA fragment into a bacterial plasmid as plasmid will be cut over the same recognition
sequence - blunt ends/sticky ends]
DNA samples from the subjects alongside a control (for size measurement) are suspended
inside holes (made by a comb) within an agarose gel plate
A negative terminal is placed adjacent to a positive terminal on either side of the agarose gel
plate. The DNA samples are located at the negative terminal
Current is switched on, and segments of DNA from each subject proceed to move towards the
opposing pole at a pace in accordance with their size and charge
After a suitable period of time, current is switched off and a DNA profile from each subject can
be obtained, and matched if required