Beruflich Dokumente
Kultur Dokumente
www.standardia.com
Chikungunya IgM
General Information
The word 'Chikungunya' is derived from the Makonde language in southeastern
Tanzania and means "bent down or become contorted," which indicates the
classic posture the patient adopts because of severe joint pain.
Chikungunya fever
Chikungunya fever is a viral disease transmitted through
the bite of infected Aedes aegypti and Aedes albopictus
mosquitoes.
http://www.cdc.gov/ncidod/dvbid/Chikungunya/
CH_Globalmap.html
Incubation period
Time from the infected bite to the onset of illness can be
3-12 days, but is usually 4-7 days.
Treatment
Currently specific drug treatment and vaccines are not
available.
Africa
Asia
Europe
Italy
--> The differential diagnosis of these two infections is essential for clinical
managent and epidemiological study in the tropics.
CHIK: Chikungunya
Detection of Chikungunya IgM in acute phase
Early detection is important in order to stop further transmission and case
management is necessary to prevent severe development of the disease.
Mosquito
Bite
viral RNA
Day 0
IgM
Day 4 to day 7
Clinical
symptoms
Day 15
Excellent performance
Internal Evaluation
Sensitivity : 97.1% (vs. ELISA)
Specificity : 98.9% (vs. ELISA)
Clinical study
According to the research performed in southern Thailand(Chikungunya outbreak, 2008), SD BIOLINE
Chikungunya IgM shows equivalent performance when compared to RT-PCR for collected CHIKV
positive samples after onset of fever. RT-PCR for CHIKV was positive in every serum obtained from
patients within the first three days after the onset of fever whereas the IgM antibody may become
detectable after 3 days of the onset of fever. In this research, author emphasizes that serological or
molecular methods are required to arrive at a definite diagnosis of either infection. RT-PCR and serology to
detect IgM antibodies or paired sera for IgG against CHIKV ought to be performed in all patients with
presumed hemorrhagic fever to detect outbreaks of CHIKV early. This precaution would help control global
epidemics of this virus.
Specification
Specimen : Serum, Plasma (50l) , Whole blood (100l)
Assay Time : 10 minutes
Detection : Chikungunya IgM antibody
Sensitivity : 97.1%, Specificity : 98.9% (vs. ELISA)
Component : Test device/ Assay diluent/ Disposable droppers
Test Procedure
Serum or Plasma
Negative
1 drop
(50l)
1 drop
10 min.
Positive
Whole Blood
Invalid
2 drops
(100l)
Reference
http://www.searo.who.int/LinkFiles/Chikungunya_FactSheet_Chikungunyafever.pdf
http://www.who.int/mediacentre/factsheets/fs327/en/
http://www.searo.who.int/LinkFiles/Chikungunya_Draft_Prevention&Control_CHIK_25_Nov.pdf
http://ecdc.europa.eu/pdf/071030CHK_mission_ITA.pdf
http://www.cdc.gov/ncidod/dvbid/Chikungunya/CH_GlobalMap.html
Ordering Information
Rapid Test
Cat. No
Product
Specimen
Type
Pack size
Storage
46FK10
Chikungunya IgM
Serum/Plasma/
Whole blood
Device
1T x 25/Kit
24 months
1~30 C
ELISA available
Cat. No
Product
Specimen
Type
Pack size
Storage
46EK10
Chikungunya IgM
ELISA
Serum
Well
96wells/Kit
12 months
2~8 C
Rev. 1310-00
Cat. No. LEF46EN