Beruflich Dokumente
Kultur Dokumente
Abstract
Background: When mineral nutrients are present in excess or in inadequate amounts, their effects
can be severe in plants and can be considered as abiotic stress. In this study, we report how
hormonal levels in barley cultivars respond to the toxic effect of boron, an essential plant
micronutrient.
Materials and Methods: Two different barley (Hordeum vulgare) cultivars (Vamik Hoca and Efes 98)
were used as a study material. Boron was applied in three different concentrations (0, 10, 20 ppm)
to plants that had grown from seeds for four weeks. Plants were harvested, stem-root length and
stem-root dry-fresh weight content were determined. For further analysis, chlorophyll, total protein,
endogenic IAA and ABA content analyses were carried out.
Results: According to the data obtained, plant growth and development decreased with increasing
boron concentrations. With increasing boron concentrations, soluble total protein increased in both
cultivars. Boron application led to increased endogenic IAA content in both cultivars. 10 and 20 ppm
boron application led to increased endogenic ABA content in Vamik Hoca cultivar whereas
endogenic ABA content decreased in Efes 98. Absence of boron application led to increased
endogenic IAA and ABA content in both cultivars.
Conclusions: As a result, the response to boron is different in the two cultivars and Efes 98 may be
more resistant to the toxicity than Vamik Hoca cultivar.
Keywords: Abscisic acid, boron toxicity, Hordeum vulgare, indole acetic acid.
Abbreviations: IAA: Indole acetic acid; ABA: Abscisic acid; TLC: Thin Layer Chromatography; D: Absorbance
values; cv: cultivated variety; B: Boron.
Ayvaz M, Koyuncu M, Guven A, Fagerstedt KV (2012) Does boron affect hormone levels of barley
cultivars? Eurasia J Biosci 6: 113-120.
DOI:10.5053/ejobios.2012.6.0.14
INTRODUCTION
Boron is an essential microelement for plant
growth and development (Warington 1923). On the
other hand toxicity and shortage range are very
narrow in plants (elik et al. 1998). Boron shortage
is widely known in soils around the world. On the
other hand toxicity is mostly seen on dry and semidry regions thus limiting plant growth and causing
yield losses (Nable et al. 1997).
In Central Anatolia boron toxicity is a problem in
agricultural soils (Torun et al. 2002). Boron toxicity
has also been reported in South Australia, in
Mediterranean countries, in California and in Chile
(Aquea et al. 2012), causing yield losses in barley
(Cartwright et al. 1984). Boron functions as a crosslinker for rhamnogalacturonan-II in the cell
membrane, and also as a component important for
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(D 663)].(V/1000. Weight)
mg total Chlorophyll/g tissue= [20.2 (D645)+8.02
(D663)].(V/1000. Weight)
Carotenoid determination
Carotenoid content was determined from the
acetone extract at 450 nm as described by Witham
et al. (1971) via a spectrophotometer. Absorbance
value (D) at 450 nm was placed in the below
equation. Carotenoid contents as mg in 1 g of plant
tissue were calculated.
mg Total carotenoid/g tissue= 4.07 (D645)
[(0.0435 Kla amount)+(0.367 Klb amount)]
Protein determination
Protein concentration was evaluated by the
method of Bradford (1976) using bovine serum
albumin as a standard.
RESULTS
Hordeum vulgare cv. Vamik Hoca and cv. Efes 98
cultivated in 0, 10 and 20 ppm boron concentration
were harvested after 4 w. According to our results;
root length and shoot height of excess boron
applied to Efes 98 and Vamik Hoca cultivars
decreased with increasing boron (Table 1).
Increasing boron conditions led to decreased fresh
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standart error, values are in centimeter (cm), cv: cultivated variety, B: boron
Table 2. Fresh and dry weight of barley cultivars grown under different boron concentrations.
standart error, values are in grams (g), cv: cultivated variety, B: boron
Table 3. Indole acetic acid and abscisic acid content of different barley cultivars grown under different boron
concentrations.
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DISCUSSION
One of the symptoms of boron toxicity is
inhibition of root growth (Nable 1988, Reid et al.
2004, Choi et al. 2007). Toxicity also causes yield
losses in barley (Cartwright et al. 1984). In a study of
optimizing growth conditions for Brassica oleracea, 1
ppm boron applied plants had the maximum fresh
weight (Shelp and Shattuck 1987). On the other
hand, plants grown in boron deficient conditions did
not show any negative effect on vegetative growth,
whereas plants faced problems in reproductive parts
(Mozafar 1993). According to our results, root length
and shoot height of excess boron applied Efes 98
and Vamik Hoca cultivars decreased with increasing
boron. In both cultivars under boron deficient
conditions, fresh weight did not change compared to
control. Therefore, our results are in line with the
above literature showing increasing boron
concentration led to decreased fresh weight in both
Ayvaz et al.
Table 4. Chlorophyll a, chlorophyll b content of barley cultivar leaves grown under different boron concentrations.
Table 5. Carotenoid and total chlorophyll content of barley cultivar leaves grown under different boron concentrations.
cultivars.
Triticum durum Desf. grown under boron
deficiency led to increased IAA content and it
tended to decrease with increasing boron
concentrations (Gemici et al. 2002). In sunflower
(Helianthus annuus L.) IAA content decreased under
boron stress in comparison to the control plants
(Akam-Oluk and Demiray 2004). According to
Dugger (1983) IAA and IAA oxidase levels changed in
boron deficient conditions: IAA oxidase activity
decreased and IAA contrarily increased (Paull et al.
1992, Bryant and Lane 1979). Under boron deficient
conditions, chlorogenic and caffeic acids accumulate
and this inhibits IAA oxidase activity, leading to auxin
accumulation in the plant tissue (Gupta 2006). In our
study, both cultivars of boron deficient group had
higher endogenic IAA levels than boron applied
groups. Our results are consistent with previous
studies indicating that in excess boron conditions,
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