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FORM A

BCH 3053 Introductory Biochemistry Spring, 2014


Exam 1, January 31
Choose the best answer from the multiple choices.
Question #21 is Extra Credit

pH= pK a +log

[ conjugate base ]
[ Conjugate acid ]

+
H

pH =log

FORM A

G=H T S

FORM A

1. A spontaneous reaction is:


a. Exergonic
b. Endergonic
c. At equilibrium
d. Always rapid
e. Always accompanied by a negative enthalpy
2. Which of the following processes results in a decrease on entropy?
a. A liquid is volatilized
b. An ice cube melts
c. Water freezes
d. Urea dissolves in water
e. This reaction occurs: CaCO3(s) CaO(s)+CO2(g)
3. What is the pH of an acetic acid solution where the concentration of acetic acid is 2mM and
concentration of sodium acetate is 10mM. The pKa of acetic acid is 4.76.
a. 3.07
b. 5.46
c. 4.06
d. 6.21
e. 3.76
4. Pick the correct conjugate acid for the conjugate bases shown below in bold:
Bases

(HO CH2)3C-NH2

H3C-CHOH-COO-

NH3

Na2HPO4

a.

(HO CH2)3C-NH4+

H3C-CHOH-COO2-

There is none

NaH2PO4

b.

((H2+) O CH2)3C-NH4+

H3C-CHOH-COOH

NH2-

NaH3PO4

c.

(HO CH2)3C-NH4+

H4+ C-CHOH-COO-

There is none

NaH3PO4

d.

(HO CH2)3C-NH

e.

(HO CH2)3C-NH3+

H3C-CHOH-COO

2-

H3C-CHOH-COOH

NH3

H3PO4

NH4+

NaH2PO4

5. Refer to figure 1. Which of the groups could not act as a


proton acceptor in a hydrogen bond
a. I
b. II
c. III
d. IV
e. All can accept a hydrogen in a hydrogen bond
BOTH ANSWERS WILL BE CORRECT

Figure 1

H3N-CH2-COO-

H3N-CH2-COOH
H3N-CH2-COO-

H4N-CH2-COOH
H2N-CH2-COO-

H3N-CH2-COOH

FORM A

6. An HCl solution has a pH=3. If you dilute 10mL of the solution to 1000mL, the final pH will be
a. 1.0
b. 2.0
c. The pH does not change
d. 4.0
e. 5.0
7. Calculate the final pH of a solution made by the addition of 10 mL of a 0.5 M NaOH solution to 500 mL
of a weak acid, HA. The concentration of the conjugate acid is 0.2M, the pH is 5.00 and the pKa = 5.00.
Neglect the change in volume
a. 6.10
b. 5.04
c. 7.00
d. 5.99
e. 6.91
8. Which of the following statements about hydrogen bonds is false?
a. The donor is a hydrogen atom bonded to a less electronegative atom than hydrogen.
b. The more linear the bond, the stronger the attraction.
c. The acceptor must contain a non-bonded pair of electrons.
d. They are a type of covalent bond
e. They are the result of dispersion forces
BOTH ANSWERS WILL BE CORRECT
9. What is the net charge of the following peptide at pH 1.00?
Cys-Ala-Gly-Arg-Gln-Met
a. +1
b. +2
c. -1
d. 0
e. -2
10. Given the R-groups in the peptide, ALA-GLN-ARG-SER-HIS, it would likely be:
a. Very water soluble
b. Somewhat water soluble
c. Not very water soluble
d. Not soluble at all
e. You cannot tell from the sequence
11. Which amino acid would migrate furthest toward the anode during electrophorese?
a. Aspartic acid
b. Arginine
c. Tyrosine
d. Histidine
e. Glutamine

FORM A

12. What is the isoelectric point of tyrosine?


a. 2.20
b. 5.65
c. 9.11
d. 10.07
e. 7.13
13. Which of the following amino acids has a net charge of +1 at pH 4 and a net charge of 0 at pH 8?
a. Glu
b. Arg
c. His
d. Tyr
e. Two of the above
14. What is the ratio of conjugate base to conjugate acid in Asp at pH = 5.2?
a. 7.6
b. 0.02
c. 12.3
d. 6.72
e. 35.48
15. Which of the following forces are involved in maintaining the primary structure of a protein?
a. Covalent bonds
b. Hydrogen bonds
c. Ionic interactions
d. Hydrophobic interactions
e. Dipole-dipole interactions
Use figure 2 to answer the questions below

Figure 2

FORM A

16. The type of bonding labeled L in figure 2 is: EVERYONE CREDITED


a. Hydrogen bonding of the peptide backbone
b. Hydrogen bonding involving the R-groups
c. Hydrophobic interactions
d. Metal ion coordination
e. Electrostatic attraction
17. The type of bonding labeled M in figure 2 is:
a. Hydrogen bonding of the peptide backbone
b. Hydrogen bonding involving the R-groups
c. Hydrophobic interactions
d. Metal ion coordination
e. Electrostatic attraction
18. The type of bonding labeled O in figure 2 is:
a. Hydrogen bonding of the peptide backbone
b. Covalent bonding involving the R-groups
c. Hydrophobic interactions
d. Metal ion coordination
e. Electrostatic attraction
19. Quaternary structure is associated with
a. The overall shape of the polypeptide chain
b. The sum of secondary and tertiary interactions
c. Simple proteins with only one subunit
d. The sequence of amino acids
e. The relative orientation of one polypeptide to another polypeptide in a multisubunit
protein
20. Which of the following statements regarding hemoglobin (Hb) and myoglobin (Mb) is true?
a. Mb transports oxygen while Hb stores it.
b. Mb has quaternary structure but Hb does not.
c. Mb displays simple kinetics of binding while Hb displays cooperativity.
d. Mb exhibits allosteric control, whereas Hb does not.
e. None of the above
21. In allosteric interactions
a. Proteins that consist of a single polypeptide chain form aggregates.
b. Disulfide bonds are broken.
c. When a small molecule binds to one site of a protein it causes changes at a distant site.
d. Metal ions always bind to the protein.
e. The sequence of amino acids is altered.