Beruflich Dokumente
Kultur Dokumente
1251-1259
Copyright @ 1983 by The American Society of Tropical Medicine and Hygiene
Abstract. A total of 46 Trypanosoma rangeli stocks were isolated from naturally infected
mammals and triatomine vectors. Twenty-two stocks were from the common opossum (Didelphis marsupialis), one from the brown "4-eyed" opossum (Metachirus nudicaudatus), one
from the anteater (Tamandua tetradactyla), one from the coati (Nasua nasua), seven from
Rhodnius pictipes and 14 from Rhodnius robustus. Two stocks were also isolated from
recently fed sandflies (Lutzomyia sp., Shannoni group). The stocks were identified as T.
rangeli on the basis of natural or experimental salivary gland infections in Rhodnius, inoculative (anterior station) transmission to mice, morphological parameters in parasitemic mice
and compari sons of isozyme profiles with a known stock of T. rangeli isolated from mano
Three other trypanosome stocks from D. marsupialis, T. tetradactyla and the three-toed sloth
(Bradypus tridactylus) were morphologically similar to T. rangeli in culture but had quite
different isozyme profiles and were not identified. It is concluded that T. rangeli is widely
distributd in Amazonas,Par and Rondonia States of Brazil, and probably extendsinto other
regions where R. pictipes and R. robustus are known to occur. R. pictipes is light-attracted
into houses and occasionally transmits Chagas' diseaseto mano It is likely that T. rangeli is
also occasionally transmitted to man in the Amazon basin.
history and biology of T. rangeli have been reviewed in detail by both Hoare4 and D' Alessandro.1
J)' Alessandro acceptsrecords of T. rangeli from
triatomine bugs and mammals in the Americas as
being biologlca1lyproven only when substantiated
by salivary gland infections or by transmissionexperiments.1Many T. rangeli-like organisms which
may undergo somedevelopmentin triatomine bugs
have been reported, and several are na:rnedas distinct species,although their relationship to T. rangeli is unclear.1,4By D' Alessandro'scriteria p~pven natural vectors of T. rangeli are R. prolixus,
R. paUescens,R. ecuadoriensis, R. pictipes, R.
robustus, Triatoma dimidiata capitata, and possibly R. brethesi.1.5 Proven naturally infected
mammal hosts are Didelphis marsupialis (common opossum),Philander opossum(Grey, "4-eyed"
opossum),Tamanduatetradactyla (anteater),Cavia
proceUus(guinea pig), Oryzomysconcolor (rice rat),
Eira barbara (tayra), Procyon lotor (raccoon),and
the primares, Cebusspp. (capuchin), Saimiri spp.
(squirrel monkey), and Saguinus (=Leontocebus)
geoffroyi (tamarin).l The known distribution of:1
l'1251
,
-;j..
1252
MILES ET AL
AND METHODS
Studyareas
Field areas are listed in Table 1 and their geographicallocations are shown in Figure 1. These
gires were selected initially because they were of
interest for researchon the mammal reservoirsand
"\
(
"
."
1253
TABLE 1
I solation histories of48* Trypanosoma rangeli stocks examined
Localityt
Host speciesDidelphis
marsuPialis I
(common opossum)
AMAZONAS
BR364 km 20-65
km 111
Estrada Belnfunte km 11
M etachirus nudicaudatus
(brown, "4-eyed" opossum)
Tamandua tetradactyla
(anteater)
Nasua nasua
(coati)
Rhodnius pictipes
RONDONIA
BR364 km 54
RONDONIA
BR364 km 92
IM1269(B)
RONDONIA
BR364 km 92
1M 1249(A)
AMAZONAS
RONDONIA
BR364 km '23
robustus
AMAZONAS
Lutzomyia sp.
(Shannoni group)
RONDONIA
BR364 km 20
,..
* Stocksderived by different routes from lhe salDe bost are considered separately"
t Soe Figure 1.
* (*) indicates stocks of T rangeli tbat were mixed with T. c",zi. ,.
(A), stocksisolated by culture of cardia.: blood from lhe natural vertebrate host on biphasic blood-agar medium;2' (B), stocksobtained by culture of
naturally infected triatomine bug fetos, salivary glands, or fetos of xenodiagnosisbugs; in addition, two stocks isolated by culture of sandfly intestinal
tra.:tz. INT = culture of intestinal tra.:t or fetos; GL ~ culture of salivary glands; (C), stocksobtained by transmissionto mito during feeding of naturally
infected bugs or by intraperitoneal inoculation of infected xenodiagnosisbug fetos, and subsequentlycultured from infected mouse cardia.: blood.
" Prevalence of T. rangeli infection in D. marsupialis was 10-30% in localities listed.
Rhodnius
251
ESULTS
MILES ET AL.
Enzymeelectrophoresis
Bulk growth of T. rangeli stocks was in large
tubes of blood-agar medium.21 Harvesting of organisms, preparation of enzyme extracts and enzyme electrophoresis were as used for the char-
1255
TABLE 2
Morphological comparisonsbetweenBrazilian T. rangeli stocks,T. rangeli isolatedfrom man, and published records
Stock code
No.
mea- .
sured*
Parameterst
L
PK
KN
20
28.3-36.7
27
27.7-35.3
25
27.0-37.0
10
25.7-33.3
(30.8)
(3.2)
(9.9)
(7.9)
R1625 (man)
23
28.0-36.0
2.7-4.0
9.3-12.7
6.3-11.7
(3.3)
(10.7)
(8.8)
(32.7)
(31.7)
(29.1)
(32.1)
Various
Variousll
(27.0-32.2)
25.0-37.0
2.7-4.3
7.0-11.7
7.0-12.0
(3.4)
(9.4)
(10.4)
2.7-4.3
8.0-10.7
6.7-12.3
(3.5)
(9.6)
(9.2)
1.7-5.7
7.0-12.7
6.6-11.7
(3.3)
(9.2)
(9.1)
2.7-4.0
7.0-12.0
5.0-10.6
7.0-11.0
(9.6)
7.3-13.0
(9.3)
5.0-10.3
(7.5)
7.3-12.7
NI
1.0-1.8
KI
1.3-1.5
(1.3)
(1.4)
1.0-2.0
1.3-1.5
(1.5)
0.9-2.2
(1.4)
1.2-1.5
(1.4)
(1.4)
1.-2.7
1.2-1.5
(9.7)
(1.8)
(1.3)
7.0-10.7
1.1-2.2
1.2-1.4
(9.4)
1.8-7.0
---(7.9-9.5)8.2-10.0
(1.6)
(1.2-1.7)
25.0-37.0
1.8-7.0
8.2-10.0
5.0-12.0
5.0-11.0
-1.1-2.8
(26.4-33.8)
(3.4-4.4)
(9.5-9.7)
(6.9-8.9)
(8.1-9.5)
-(1.6-2.0)
(1.3)
(1.6-2.0)
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MILES ET AL.
FIGURE2. Examples of isozyrneprofiles. The enzyrnesshown are: (a) ME; (b) ASATj (c) PGM; .and (d) GPI.
In each casethe trypanosomestocks are (from left to right): I, R 1625-Trypanosoma rangeli from man, EI Salvador;
2, IM92-T. rangeli from Didelphis marsupialis, Rondonia State, Brazilj 3, Bug 333/9 GL-T. rangeli from Rhodnius PictiPes, Manaus, Amazonas State, Brazil; 4, Bug 1752 GL-T. rangeli from Rhodnius robustus, Manaus,
Amazonas State, Brazil; 5, IMI087-T. rangeli from Lutzomyia sp. (Shannoni group), Rondonia State, Brazilj 6,
IM418-unidentified, from Bradypus tridactylus, Manaus, Amazonas State, Brazilj 7, 1M 1269-T. rangeli from
Tamandua tetradactyla, Rondonia State, Brazil. Scale: the application threads at the origin measureapproximately
1 cm.
tion.
t INPA Manaus stock code numbers.
DISCUSSION
zil.
1257
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MILES ET AL.
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