Beruflich Dokumente
Kultur Dokumente
ORIGINAL ARTICLE
ABSTRACT
The aim of the study was to ascertain the role of ethanolic extract of Cynodon dactylon against hepatic
complications in streptozotocin (STZ) induced type 2 diabetic models. Effect of the pre identified most effective
dose of 500 mg/kg body weight was studied on hepatic injury caused by chemically induced diabetes by 55
mg/kg body weight i.p. injection of STZ in male Wistar rats. The dose of 500mg/kg body weight given once
daily for 14 days reduced the levels of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate
transaminase, alkaline phosphatase, creatinine and urine sugar significantly (P<0.05) with increase in total
protein, haemoglobin and body weight was increased. High LD50 validates its high margin of safety.
KEY WORDS
Antidiabetic effect, Cynodon dactylon, Diabetes, Hepatoprotective.
INTRODUCTION
The popularity of plant-based drug in the modern system of
medicine is growing day by day as they are claimed to be
safe, economical and yet efficacious (1-3). The adverse drug
reaction of synthetic medicines in the treatment of diabetes
mellitus has restricted in a growing demand for the use of
herbal drug or Phytomedicines (4). However, there is still an
unmet need for improved oral drugs from medicinal plants for
Diabetes Mellitus.
The Cynodon dactylon (Family: Poaceae) commonly known
as Doob; Hindi Aroogum pillo; Tamil, Garike; Telulgu and
Bermuda grass; English in India is a creeper. It is a weed
and has been regarded to possess varied medicinal properties
(5). The aqueous fluid extract of the rhizome is used as anti-
Table 1: Effect of administration of aqueous extract of Cynodon dactylon on SGOT and SGPT levels in normal and severely diabetic rats
Groups
Treatment
SGOT (U/L)
SGPT (U/L)
Before treatment
Group I
Distilled water
66.7 3.2
65.8 3.8
29.2 1.4
28.8 2.4
Group II
Extract
73.7 2.8
58.2 3.4*
27.9 1.2
20.8 1.8*
Group III
Distilled water
103.8 2.8
120.5 3.5*
51.7 6.5
63.5 5.1*
Group IV
Extract
122.6 4.2
85.5 4.1*
52.2 7.3
44.5 1.5*
* P < 0.05 as compared to initial value. Group 1 Normal Control (Treated with distilled water), Group 2 Normal (Treated with 500 mg/kg extract),
Group 3 Diabetic Control (Treated with distilled water), Group 4 Diabetic (Treated with 500 mg/kg extract).
Table 2: Effect of administration of aqueous extract of Cynodon dactylon on serum alkaline phosphatase and
serum creatinine levels in normal and severely diabetic rats
Groups
Treatment
ALP (U/L)
Before treatment
CREA (mg/dl)
Before treatment
Group I
Distilled water
77.1 5.6
78.2 5.2
0.98 0.14
1.10 0.16
Group II
Extract
72.9 6.4
48.8 7.2*
1.070.18
0.88 0.15*
Group III
Distilled water
147.6 3.2
154.8 7.2*
1.70 4.2
2.10 3.4*
Group IV
Extract
145.2 6.5
102.5 8.2*
1.61 2.2
0.94 3.8*
* P < 0.05 as compared to initial value. Group 1 Normal Control (Treated with distilled water), Group 2 Normal (Treated with 500 mg/kg extract),
Group 3 Diabetic Control (Treated with distilled water), Group 4 Diabetic (Treated with 500 mg/kg extract).
411
Table 3: Effect of administration of aqueous extract of Cynodon dactylon on haemoglobin and protein levels in
normal and severely diabetic rats
Groups
Group I
Treatment
Distilled water
Before treatment
Before treatment
13.2 1.6
13.5 1.7
7.5 3.2
7.8 2.3
Group II
Extract
12.8 2.1
14.6 2.4
6.8 5.6
7.4 3.2
Group III
Distilled water
12.1 3.5
10.5 1.6
7.8 3.4
7.1 6.4
Group IV
Extract
12.5 6.5
14.8 4.1*
7.5 1.2
8.9 4.2*
*P < 0.05 as compared to initial value. Group 1 Normal Control (Treated with distilled water), Group 2 Normal (Treated with 500 mg/kg extract),
Group 3 Diabetic Control (Treated with distilled water), Group 4 Diabetic (Treated with 500 mg/kg extract).
DISCUSSION
Cynodon dactylon is traditionally used in India as a therapeutic
agent to control diabetes mellitus. Our earlier reports on
Table 4: Effect of administration of aqueous extract of Cynodon dactylon on body weight and urine sugar levels in
normal and severely diabetic rats
Groups
Group I
Treatment
Distilled water
Before treatment
Before treatment
190.0 8.6
220.8 9.4
Nil
Nil
Group II
Extract
198.6 7.8
224.4 8.2
Nil
Nil
Group III
Distilled water
216.5 8.2
165.2 5.2
+4
+4
Group IV
Extract
188.2 6.2
205.6 5.8*
+4
+1**
*P < 0.05 as compared to initial valve; ** P < 0.01 as compared to initial value. Group 1 Normal Control (Treated with distilled water), Group
2 Normal (Treated with 500 mg/kg extract), Group 3 Diabetic Control (Treated with distilled water), Group 4 Diabetic (Treated with 500 mg/kg
extract).
412
4.
5.
Kritikar KK, Basu BD. Indian Medicinal Plants, 2nd ed. India:
1980: 2650pp.
6.
7.
8.
9.
ACKNOWLEDGEMENTS
REFERENCES
1.
Rai PK, Rai NK, Rai AK, Watal G. Role of LIBS in elemental
analysis of P. guajava responsible for glycemic potential.
Inst Sc Tech 2007b; 35 (5): 507-22.
2.
3.
413