Food Testing

Food Testing
Food Safety and Quality Diagnostics

Bio-Rad Solutions
17933 - Rev H - User Guide Food Safety V10.indd 1
02/01/14 16:07
Contents
PART 1 Routine Testing - Quality Indicator
Preparation of dilutions
Coliforms - ISO standards
Total flora
Coliforms - Alternative method: RAPIDE.coli 2 Agar
Escherichia coli - ISO standards
Enterobacteriaceae
Escherichia coli - Alternative method:

RAPIDE.coli 2 Agar
Enterobacteriaceae - Alternative method:

RAPIDEnterobacteriaceae 9
PART 2 Pathogen Testing

Salmonella - ISO standard
10
10
Listeria spp. - Alternative method:

iQ-Check Listeria spp. real-time PCR Kit
22
11
Escherichia coli O157:H7 - Alternative method:

RAPIDE.coli O157:H7 Agar
23
12
STEC - iQ-Check STEC VirX and SerO

real-time PCR Kits
24
24
FDA-BAM Salmonella protocol 11

FSIS Salmonella MLG 4.07
Salmonella - Alternative method:
RAPIDSalmonella Agar double enrichment protocol
RAPIDSalmonella Agar short protocol
12

iQ-Check Salmonella II real-time PCR Kit

iQ-Check E.coli O157:H7 real-time PCR Kit
12
Campylobacter spp. - ISO standard
25
Cronobacter spp. - ISO standard
14
FDA-BAM Campylobacter spp. protocol
26
14
Campylobacter spp. - Alternative method:

iQ-Check Campylobacter real-time PCR Kit
26
15

RAPIDCampylobacter spp.
27
15
Staphylococcus aureus - ISO standards
28
16
FDA-BAM Staphylococcus aureus 29
FDA-BAM Cronobacter spp.

Cronobacter spp. - Alternative method:
RAPIDSakazakii Agar short protocol
iQ-Check Cronobacter spp. real-time PCR Kit
Listeria monocytogenes - ISO standards
FDA-BAM Listeria monocytogenes protocol 18

Health Canada Listeria monocytogenes
protocol MFHPB-30
Listeria monocytogenes - Alternative method:
RAPIDL.mono Agar
18
29
Sulfite-reducing bacteria
30
Clostridium perfringens 30
19

iQ-Check Listeria monocytogenes II real-time PCR Kit 20
Listeria spp. - Alternative method: RAPIDL.mono Agar 21
RAPIDListeria spp. Agar
Staphylococcus aureus - Alternative method:

RAPIDStaph Agar
Bacillus cereus
31
Shigella 31
Validations 32
Range for Food Testing
33
21
This booklet is only an indication provided by Bio-Rad for the use of Bio-Rad products.
It is not a contractual document.
02/01/14 16:07
Routine Testing Quality Indicator
Preparation of dilutions
PCA
Bacteria
Ordering Information
Catalog #
Description
NF EN ISO 6887-1 (09/1999)
NF EN ISO 4833 (05/2003)
Preparation of test samples, initial

suspension and decimal dilutions
for microbiological examination
General guidance for the

enumeration of micro-organisms
Part 1: General rules for

preparation of dilutions for
microbiological examination
Colony-count technique at 30C
m g or v ml of sample
+
9 x m g or 9 x v ml of
Tryptone Salt or Buffered
Peptone Water
(and following dilutions)
Buffered Peptone Water/Broth

Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Dehydrated
356-4684
500 g
Total flora
Initial suspension
and/or
decimal dilutions
PCA
Dilution 10-1
PCA (Plate Count Agar)/Agar

Ready to use
355-4459
100 ml x 6 bottles
355-4457
200 ml x 6 bottles
356-3989
90 mm x 20 dishes
Dehydrated
356-4475
500 g
Tryptone-Salt/Diluent
Ready to use
355-5754
9 ml x 25 tubes
355-5756
90 ml x 6 bottles
355-5796
3 L x 4 bags
Dehydrated
356-4544
30C
72 hrs
(and following dilutions)
Enumeration
500 g
Food Safety and Quality Diagnostics Bio-Rad Solutions |
| 3
02/01/14 16:07
Escherichia coli - ISO standards

Catalog #
Description

Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Dehydrated
356-4684
ISO 16649-2 (04/2001)
Horizontal method for the enumeration Horizontal method for the enumeration
of -glucuronidase-positive E. coli of -glucuronidase-positive E. coli
Part 1: Colony-count technique at Part 2: Colony-count technique at
44C using membranes and
44C using 5-bromo-4-chloro5-bromo-4-chloro-3-indolyl3-indolyl- -D-glucuronide
-D-glucuronide
500 g
EC Medium/Broth
Dehydrated
356-4764
500 g
Kovacs Reagent
(for Indole detection)
355-5313
15 ml x 2 bottles
Peptone Water (Indole Free)/Broth
Ready to use
355-4180
9 ml x 25 tubes
Dehydrated
356-4334
ISO 16649-1 (04/2001)
Dilutions
Dilutions
Dehydrated
356-4024
500 g
TBX/Agar
Ready to use
355-5309
355-5307
100 ml x 6 bottles
200 ml x 6 bottles
Dehydrated
356-4035
500 g
TBX
MMGA with membrane

Room
t 15 min
and 37C
4 hrs 1 hr
Resuscitation
500 g
RAPIDE.coli 2/Agar
Ready to use
355-5297
200 ml x 6 bottles
355-5299
100 ml x 6 bottles
Sample/
initial suspension
Sample/
initial suspension
(2 X)*
TBX
44C
18-24 hrs
Direct enumeration
44C
18-24 hrs
Direct enumeration
Tryptose Lauryl Sulfate/Broth

Dehydrated
356-4774
500 g
Ready to use
355-5754
9 ml x 25 tubes
355-5756
90 ml x 6 bottles
355-5796
3 L x 4 bags
* Please refer to ISO 7218: 2007
Dehydrated
356-4544 500 g
4 |
| Food Safety and Quality Diagnostics Bio-Rad Solutions
02/01/14 16:07
Escherichia coli
Alternative method:
RAPIDE.coli 2 Agar
ISO/TS 16649-3 (09/2005)
ISO 7251 (07/2005)
Horizontal method for the enumeration

of -glucuronidase-positive E. coli
General guidance for detection

and enumeration
of presumptive Escherichia coli
Part 3: Most probable number

technique using 5-bromo-4-chloro- Most probable number technique
3-indolyl- -D-glucuronide
Sample/initial suspension
Enumeration of Escherichia coli

NF VALIDATION according to ISO 16140
N BRD 07/1-07/93 and BRD 07/7-12/04
AOAC-RI approved N 050601**
NordVal approved N 020
Health Canada (Pending)
All food stuffs
Sample/
initial suspension
Sample/
initial suspension
Dilutions
Add 10 ml in
And subsequent dilution
3 tubes of double
Add 1 ml in 3 tubes
strength MMG of single strength MMG
Tryptose Lauryl
Sulfate
RAPIDE.coli 2
37C
24 hrs 2 hrs
Single
concentration
Subculture each tube presenting

yellow coloration on a dish of TBX
44C
20-24 hrs
(or)*
Double
concentration
44C/37C
18-24 hrs
37C
24-48 hrs
2 hrs
37C
24-48 hrs
2 hrs
EC Medium
Direct enumeration
of typical colonies*
44C
24-48 hrs
2 hrs
Each tube showing the presence of

blue colonies on TBX
should be considered positive
Peptone Water
44C
48 hrs
2 hrs
Indole test reading and calculation

Calculate the most probable number
from the number of positive tubes
at each dilution
* For detection method only
* Enumeration of typical colonies for E. coli

at 44C; for E. coli and coliforms at 37C
** Please refer to AOAC certificate for
validated matrices
| 5
02/01/14 16:07
Coliforms - ISO standards

ISO 4831 (08/2006)
Microbiology of food and animal feeding stuffs
Horizontal method for the detection
and enumeration of coliforms
VRLB
Coliforms
MPN technique
Catalog #
Description
BGBLB (Brilliant Green Bile

Lactose Broth)/Broth
Ready to use
357-8024
10 ml x 25 tubes
Sample
Dilutions

Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Dehydrated
356-4684
500 g
RAPIDE.coli 2/Agar
Ready to use
355-5297
200 ml x 6 bottles
355-5299
100 ml x 6 bottles
Dehydrated
356-4024
Tryptose Lauryl Sulfate
Single
concentration
(or)*
30C or 37C
24-48 hrs 2 hrs
30C or 37C
24 hrs 2 hrs
500 g
Tryptose Lauryl Sulfate/Broth

Dehydrated
356-4774
500 g
BGBLB
Ready to use
355-5754
9 ml x 25 tubes
355-5756
90 ml x 6 bottles
355-5796
3 L x 4 bags
Dehydrated
356-4544
Double
concentration
30-37C
24 hrs (+ 24 hrs)
500 g
Reading (gas +)
VRBL (Violet Red Bile Lactose)/

Agar
Ready to use
355-4799
100 ml x 6 bottles
Dehydrated
356-4594
6 |
500 g
02/01/14 16:07
Coliforms
Alternative method:
RAPIDE.coli 2 Agar
ISO 4832 (02/2006)
Microbiology of food and animal
feeding stuffs
Horizontal method
for the enumeration of coliforms
Enumeration of coliforms
N BRD 07/8-12/04
AOAC-RI approved N 050601**
Heath Canada (Pending)
All food stuffs
RAPIDE.coli 2
Colony-count technique
Sample/
initial suspension
Dilutions
E. coli
Other
Coliforms
Total Coliforms
Sample/
initial suspension
Dilutions
VRBL
RAPIDE.coli 2
(2 X)*
30-37C
24 hrs
2 hrs
Enumeration
of characteristic colonies
and confirmation
for atypical colonies
37C
18-24 hrs
Direct enumeration
of typical colonies*
Coliforms
Diameter 0.5mm
Purple with or without a red
zone of precipitated bile
* Enumeration of typical colonies for E. coli

at 44C; for E. coli and coliforms at 37C
** Please refer to AOAC certificate for
validated matrices
| 7
02/01/14 16:07
Enterobacteriaceae
ISO 21528-1 (08/2004)
Horizontal methods for the detection
and enumeration of Enterobacteriaceae
VRBG
Enterobacteriaceae
Part 1: Detection and enumeration by MPN technique

with pre-enrichment
Sample
Non selective
Pre-enrichment
Buffered Peptone Water

37C
18 hrs 2 hrs
Catalog #
Description

Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Dehydrated
356-4684
500 g
EE/Broth
Dehydrated
356-4794
500 g
EE
Nutrient (2.8%)/Agar
Ready to use
355-4478
8 ml x 25 tubes
Dehydrated
356-4484
500 g
Oxidase Test
355-3834

2 x vials of
50 oxidase disks
VRBG (Violet Red Bile Glucose)/

Agar
Ready to use
355-4239
100 ml x 6 bottles
Dehydrated
356-4584
500 g
8 |
37C
24 hrs 2 hrs
Selective
enrichment
VRBG
37C
24 hrs 2 hrs
Confirmation
Nutrient (2.8%)
37C
24 hrs 2 hrs
Biochemical confirmation
Oxidase
Dextrose BCP
37C
24 hrs 2 hrs
Yellow colour (+)
02/01/14 16:07
Enterobacteriaceae
Alternative method:
RAPIDEnterobacteriaceae
ISO 21528-2 (08/2004)
feeding stuffs
Horizontal methods for the detection
and enumeration of Enterobacteriaceae
Enumeration of
Enterobacteriaceae
NF VALIDATION according to
ISO 16140
N BRD 07124-11/13
Enterobacteriaceae
Non Enterobacteriaceae
Part 2: Colony-count technique
Dilutions
Sample/
initial suspension
Dilutions
VRBG
(2 X)*
Catalog #
37C
24 hrs
2 hrs
37C**
1C
24 hrs
2 hrs
Reading
Confirmation
Nutrient (2.8%)
Direct enumeration*
37C
24 hrs
2 hrs
RAPIDEnterobacteriaceae/
Agar
Ready to use
355-4012
200 ml x 6 bottles
Dehydrated
356-4004
500 g
Ready to use
355-5754
9 ml x 25 tubes
355-5756
90 ml x 6 bottles
355-5796
3 L x 4 bags
Dehydrated
356-4544 500 g
Biochemical confirmation
Oxidase (-)
Description
Dextrose BCP
37C
24 hrs
2 hrs
Yellow colour (+)

* Suitable with an automatic plate counter

** Alternatively, a temperature of 30C or
35C can be chosen
| 9
02/01/14 16:07
Salmonella - ISO standard

EN ISO 6579/A1 (02/2006)
Horizontal method for the detection of Salmonella spp.
Amendment 1 Appendix D: Detection of Salmonella spp. in animal faeces and in samples
from primary production stage
Sample
Non selective
pre-enrichment

Selective
enrichment
37C 1C
18 hrs 2 hrs
Appendix D Protocol
Rappaport
Vassiliadis Soya
MSRV
MKTTn
37C 1C
24 hrs 3 hrs
41.5C 1C
24 hrs 3 hrs
41.5C 1.5C
24 hrs 3 hrs
(+24 hrs for negative)
Plating-out
Selective
isolation
XLD
RAPIDSalmonella
or XLT 4
or Kristensen
or Hektoen
37C 1C
24 hrs 3 hrs
Confirmation
Nutrient 2.1%
37C 1C
24 hrs 3 hrs
Confirmation
Biochemical and serological confirmation

TSI Agar and other test
10 |
02/01/14 16:07
Pathogen Testing
FDA-BAM Salmonella protocol
FSIS Salmonella MLG 4.07
Sample
Sample
Non selective
pre-enrichment
Non selective
pre-enrichment
Pre-enrichment depend
of the matrice
Lactose Broth
or Trypticase Soy Broth with/without Ferrous Sulfate
or Universal Prenrichment Broth with/without Ferric
Ammonium Citrate
or Buffered Peptone Water
37C
24 hrs 2 hrs
Selective
enrichment
Rappaport
Vassiliadis Soya
and
Tretiationate
Broth
42C
24 hrs 2 hrs

or mTSB (for raw beef, carcass and environmental samples)
37C
18-24 hrs
Selective
enrichment
Modified
Rappaport
Vassiliadis Broth
Depending on the
food
and
Tetrathionate
Broth
42C
22-24 hrs
35C or 43C
24 hrs 2 hrs
Plating-out
Selective
isolation
Plating-out
Brillant Green
Sulfat Agar
Selective
isolation
XLD
Hektoen
and
XLT4 or
DMLIA
Bismuth
Sulfite
35C
18-24 hrs
37C 1C
24 hrs 3 hrs
Confirmation
Confirmation
TSI and LIA Agar

TSI and LIA Agar
| 11
02/01/14 16:07
Salmonella - Alternative
method: RAPIDSalmonella Agar
double enrichment protocol
Salmonella - Alternative
method: RAPIDSalmonella Agar
short protocol

iQ-Check Salmonella II
real-time PCR Kit
Detection of Salmonella spp.

ISO 16140
N BRD 07/11-12/05
AOAC-RI approved N 0507011*
All food stuffs and feed

Certification by NF VALIDATION
according to ISO 16140
N 07/11-12/05
AOAC-RI approved N 050701

N BRD 07/06-07/04
Health Canada approved N MFLP-38
All foods stuffs1,2, feed, and environment
including primary production
Sample
Sample
Sample

Buffered
Peptone Water

+
Salmonella Capsule
Supplement
37C
18 hrs
2 hrs
Non selective
pre-enrichment
Rappaport
Vassiliadis Soya
41.5C
1C
18 hrs
2 hrs
Selective
enrichment
41.5C
6-26 hrs
Selective
enrichment
37C
From 10 hrs 2 hrs
to 21 hrs 1 hr
Bacterial Lysis & DNA Extraction

(Standard or Easy)
Real-time PCR amplification and

detection
with iQ-Check Salmonella II
Plating-out
Plating-out
RAPIDSalmonella
RAPIDSalmonella
Selective
isolation
Selective
isolation
37C
24 hrs
2 hrs
Reading & confirmation

Standard tests
or Oxidase/OMNI-O/ONPG
or iQ-Check Salmonella II
Reading & confirmation

Salmonella Latex or Oxidase/
OMNI-O/ONPG
or iQ-Check Salmonella
* Please refer to AOAC certificate for

validated matrices
For more information on Bio-Rads real-time PCR kits and instruments, please
contact your local Bio-Rad sales representative.
1 - For AOAC-RI protocol, please contact your local Bio-Rad sales representative
2 - 8-hr enrichment protocol available for raw meat (AOAC and AFNOR validated)
12 |
37C
1C
24 hrs
2 hrs
Automatic result analysis

and interpretation
+/Confirmation of positive with

RAPIDSalmonella
validated matrices
Purchase of iQ-Check Kits includes an immunity from suit under patents specified in the product insert to use only the amount purchased solely in food
testing, environmental testing, industrial microbiology and also for the purchasers own internal research. No other patent rights (such as 5 Nuclease
Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting
the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
02/01/14 16:07
Pathogen Testing
Salmonella spp.
RAPIDSalmonella
Other
Enterobacteriaceae
MSRV (Semi-Solid Rappaport

Vassiliadis Medium)
Ready to use
355-6139
200 ml x 6 bottles
Catalog #
Description
Antiserum Salmonella
Omnivalent OMNI-O (A60)
356-0781
3 ml dropper bottle
Brilliant Green (Kristensen)/Agar
Dehydrated
356-4464
500 g
Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Dehydrated
356-4684
500 g
Hektoen/Agar
Ready to use
355-4386
100 ml x 6 bottles
356-3894
90 mm x 20 dishes
Dehydrated
356-4325
500 g
Novobiocin/Supplement
Dehydrated
356-4610
1g
Dehydrated
356-4485
500 g
ONPG Test
355-3822
Set of 50 disks
Oxidase Test
355-3834
2 x vials of

50 oxidase disks
RAPIDSalmonella/Agar
Ready to use
356-3961
90 mm x 20 dishes
356-3963 90 mm x 120 dishes
Dehydrated
356-4705
500 g
iQ-Check Salmonella II Kit

357-8123
96 tests
RAPIDSalmonella Capsules/
Supplement
356-4709
100 x quantity for 2.5 L
356-4710
100 x quantity for 250 ml
356-4712 1 x quantity for
100 analyses
MKTTN (Muller Kauffmann

Tetrathionate Novobiocin)/Broth
Ready to use
355-6140
10 ml x 50 tubes
RVS/Broth
(Rappaport Vassiliadis Soy)
Ready to use
355-5773
10 ml x 25 tubes
Dehydrated Base
356-4714
500 g
Dehydrated
356-4324
Dehydrated
356-4284
500 g
500 g
Salmonella Latex Kit

Detection of groups B to E and G
355-6710
75 tests
Salmonella Confirm Latex Kit
355-6711 50 tests
Selenite-Cystine/Broth
Complete medium
355-5746
10 ml x 25 tubes
TSB/Broth
Ready to use
(containing novobiocin)
355-5426
225 ml x 6 bottles
Dehydrated Base
356-4426
500 g
TSI (Triple Sugar Iron)/Agar
Dehydrated
356-4384
500 g
XLD (Xylose Lysine
Desoxycholate)/Agar
Ready to use
354-1751
90 mm x 20 dishes
Dehydrated
356-9124
500 g
XLT 4 (Xylose Lysine

Tergitol 4)/Agar
Ready to use
356-3654
90 mm x 20 dishes
| 13
02/01/14 16:07
Cronobacter spp. - ISO standard
Cronobacter spp.
ISO/TS 22964:2006
Milk and milk products Detection of Enterobacter
sakazakii
FDA BAM Cronobacter spp.

protocol
Samples
36C 1C
24 hrs 2 hrs
Incubation
RAPIDSakazakii
Other
Enterobacteriaceae
1:10
Sample preparation
Weigh z g of the test portion
in 9 x z ml Buffered Peptone
Water
40 ml
37C
18 hrs 2 hrs
Selective enrichment
Transfer 0.1 ml in 10 ml
of mLST/vancomycin medium
Catalog #
Description

Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Dehydrated
356-4684
44C
24 hrs 2 hrs
Streak onto RAPIDSakazakii

44C
24 hrs 2 hrs
500 g
iQ-Check Cronobacter spp.

357-8137
96 tests
14 |
DFI
Agar
Incubation
44C
48 hrs
500 g
Trypto-Casein-Soy (TSA)/Agar
Ready to use
356-3884
90 mm x 20 dishes
355-5947
200 ml x 6 bottles
355-5944
8 ml x 25 inclined tubes
Spread and
streak plates
100 l
R&F
Agar
PCR PCR
Transfer 1 to 5 colonies on TSA
RAPIDSakazakii/Agar
Ready to use
356-3971
90 mm x 20 dishes
Dehydrated
356-4976
Centrifuge 3.000 x g for 10 min.

decant supernatant
Resuspend pellet in 200 l PBS
Perform identification
tests on yellow colonies
36C 1C
18-24 hrs
Typical colonies from each agar

are confirmed with real-time PCR
and other technique
02/01/14 16:07
Pathogen Testing
Cronobacter spp. - Alternative

method: RAPIDSakazakii Agar
short protocol

iQ-Check Cronobacter spp. real-time PCR Kit
RAPIDSakazakii short protocol

ISO 16140
N BRD 07/22-05/12
Sample preparation
Weigh z g of the test portion
in 9 x z ml BPW
37C
18 hrs 2 hrs
Detection of Cronobacter spp.

Infant Formulas and environment
NF VALIDATION pending
Environmental sample

37C
18 hrs 2 hrs
Infant formula
Suppl. Buffered Peptone Water

37C
20 hrs 2 hrs

37C
4 hrs 1 hr
Streak onto RAPIDSakazakii

44C
24 hrs 2 hrs

(Standard or Easy)
Real-time PCR amplification and detection

with iQ-Check Cronobacter spp.
Transfer 1 to 5 colonies on TSA
25C
48-72 hrs
Perform identification
tests on yellow colonies
Automatic result analysis and interpretation
+/Confirmation of positive with RAPIDSakazakii
| 15
02/01/14 16:07
Listeria monocytogenes - ISO standards

EN ISO 11290-1/A1 (02/2005)
Horizontal method for the detection and enumeration of Listeria monocytogenes
Part 1: Detection method
Sample
Fraser
Primary enrichment
Isolation
Secondary enrichment
Fraser 1
(0.1 ml culture/10 ml)
Other solid
selective medium
Agar Listeria according

to Ottaviani and Agosti
37C 1C
24 hrs 3 hrs
(+28 hrs 2 hrs)*
35C or 37C
48 hrs 2 hrs
30C-35C-37C
Isolation
(depending
of the media)
Agar Listeria
according
to Ottaviani
and Agosti
24-48 hrs 2 hrs
Reading and
confirmation
Listeria spp.
-
TSYEA agar
Catalase +
Gram
Mobility
30C
24 hrs 2 hrs
Listeria
monocytogenes
-
Other solid
selective
medium
37C 1C
24 hrs 3 hrs
(+24 hrs 3 hrs)
TSYEB
Hemolysis
Carbohydrates
Camp test
30C-35C-37C
(depending
of the media)
24-48 hrs 2 hrs
Reading and
confirmation
* If low or absent colony growth observed after 24 hours.
16 |
02/01/14 16:07
Pathogen Testing
Catalog #
EN ISO 11290-2/A1 (02/2005)

Horizontal method for the detection and enumeration
of Listeria monocytogenes
Supplement 1
356-4201
100 capsules

(1 capsule qsp 250 ml)
356-4041
10 vials
Sample
Supplement 2
356-4042
10 vials
Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Dilution
Fraser without LiCl

and selective supplement
or Buffered Peptone Water
Dehydrated
356-4684
20C 2C
1 hr 5 min
Agar Listeria according

to Ottaviani and Agosti
Enumeration and confirmation tests
TSYEA agar
Catalase +
Gram
Mobility
Listeria
monocytogenes
-
TSYEB
Hemolysis
Carbohydrates
Camp test
500 g
Fraser /Broth
Complete medium
355-5797
225 ml x 6 bottles
355-5794
3 L x 4 bags
355-5792
5 L x 2 bags
Dehydrated Base
356-4604
500 g
37C
24 hrs 3 hrs
(+ 24 hrs 3 hrs)
Dehydrated Base
356-4043
500 g
Base in vials (to be supplemented)
355-5200
237.5 ml x 6 bottles
Part 2: Enumeration method
Listeria spp.
Description
AL (Agar Listeria according

to Ottaviani and Agosti)/Agar
Ready to use
356-3695
90 mm x 20 dishes
356-3965
90 mm x 120 dishes
Supplement
356-4616
10 vials
Fraser 1/Broth
Complete medium
355-4569
10 ml x 25 tubes
Dehydrated Base
356-4604
500 g
Supplement
356-4615
10 vials
Oxford/Agar
Ready to use
356-3664
90 mm x 20 dishes
Palcam/Agar
Ready to use
356-3674
90 mm x 20 dishes
Dehydrated Base
356-4754
500 g
Supplement
356-4752
10 vials
| 17
02/01/14 16:07
FDA-BAM Listeria monocytogenes protocol
Health Canada
Listeria monocytogenes
protocol MFHPB-30
Sample
Sample
BLED
LEB
30C
4 hrs
30C
24 hrs
30C
24 hrs
30C
48 hrs
+ supplements
30C
24 hrs 2 hrs (48 hrs 2 hrs)*
RAPIDL.mono
Agar Listeria
according to
Ottaviani and
RAPIDL.mono
Agosti
Oxford
or
Palcam
Modified Oxford
or
and
(MOX)
30C
24 hrs (+24 hrs)
0.1 ml MFB
35C
24 hrs
48 hrs
0.1 ml MFB
35C
24 hrs
48 hrs
RAPIDL.mono
RAPIDL.mono
or
30C
24 hrs (+24 hrs)
30C
24 hrs
(+ 24 hrs)
30C
24 hrs
(+ 24 hrs)
30C
24 hrs
(+ 24 hrs)
Reading, isolation and confirmation

Reading, confirmation
and (optional)
Listeria spp.
-
TSYEA agar
Catalase +
Gram
Mobility
Listeria
monocytogenes
-
TSYEB
Hemolysis
Carbohydrates
Camp test
Real-time
PCR
amplification
or
- Motility
- Hemolysis,
- Mannitol, rhamnose
and xylose (or other
approved methods
Compendium
considered equivalent
for these steps)
- Real-Time PCR
Amplification
- Catalase
- Gram
- Serology
* If low or absent colony growth observed after 24 hours
18 |
02/01/14 16:07
Pathogen Testing
Listeria monocytogenes - Alternative method: RAPIDL.mono Agar

Detection of Listeria monocytogenes
N BRD 07/4-09/98
NordVal approved
All food stuffs and environment
Enumeration of Listeria monocytogenes

ISO 16140
N BRD 07/05-09/01
Sample
Sample
Resuscitation
Fraser Broth
Buffered
Peptone Water
30C
24 hrs
2 hrs
20C
1 hr
Isolation on
RapidL.mono
Isolation on
RapidL.mono
37C
24 hrs
2 hrs
37C
24 hrs
2 hrs
Reading and
confirmation tests
Direct
enumeration
Standard Tests
Spot on
Agar Listeria
- TSYEB
or according to
- Hemolysis
Ottaviani and
- Carbohydrates
Agosti
- Camp test
iQ-Check
or Listeria
monocytogenes II
or Rhamnose
Test
* Please refer to AOAC certificate for validated matrices
For a NF VALIDATION validated

enumeration method, in the event
of a positive result using
RAPIDL.mono for enumeration,
confirmation is not necessary when the
presence of Listeria monocytogenes
has been confirmed during detection.
For more information on Bio-Rads real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.
1-F
or AOAC-RI protocol, please contact your local Bio-Rad sales representative
| 19
02/01/14 16:07

iQ-Check Listeria monocytogenes II real-time PCR Kit
Detection of Listeria monocytogenes
N BRD 07/10-04/05
L. mono
L. ivanovii
RAPIDL.mono
L. innocua
Sample
L. welshimeri
Catalog #
(1)
Description
Listeria Special Broth (LSB) or
(2)
Fraser Broth
30C
23 hrs 1 hr (1)
or 25 hrs 1 hr (2)

Ready to use
355-4179 225 ml x 6 bottles
355-5795 3 L x 4 bags
355-5790 5 L x 2 bags

Standard (Fraser and LSB) or Easy (LSB)
Dehydrated
356-4684 500 g
iQ-Check
Listeria monocytogenes II Kit
357-8124 96 tests
Real-time PCR amplification and detection with

iQ-Check Listeria monocytogenes II
LSB (Listeria Special Broth)

Ready to use
355-5703 225 ml x 6 bottles
355-5793 5 L x 2 bags
Dehydrated
356-4703 500 g
356-4753 5 kg
RAPIDL.mono/Agar
Ready to use
356-3964 90 mm x 120 dishes
356-3694 90 mm x 20 dishes
355-5294 Kit: 190 ml agar + 2 suppl.
+/-
Dehydrated Base
356-4293 500 g
Supplement 1
356-4294 10 vials
Confirmation of positive with RAPIDL.mono
Supplement 2
356-4746 10 vials
Rhamnose Test
355-3669 28 tests
20 |
02/01/14 16:07
Pathogen Testing
Listeria spp.
Alternative method:
RAPIDL.mono Agar
Listeria spp. - Alternative method: RAPIDListeria spp. Agar
Detection of Listeria spp.

ISO 16140
N BRD 07/4-09/98

N BRD 07/12-12/06
Sample
Sample
Fraser Broth
Fraser Broth or UVM

30C
24 hrs 2 hrs
30C
24 hrs
2 hrs
Isolation on
RapidListeria spp.
Isolation on
RapidL.mono
37C
24 hrs 2 hrs
37C
24-48 hrs
Reading and
confirmation tests
Reading and confirmation tests
Standard Tests
- TSYEA
- Gram
- Catalase
or iQ-Check
Listeria spp.
Standard Tests
- Gram
- Catalase
Spot on
or RAPIDL.mono or Spot on Palcam or iQ-Check
+ Gram test
+ Catalase
Listeria spp.
| 21
02/01/14 16:08

iQ-Check Listeria spp. real-time PCR Kit
N BRD 07/13-05/07
Health Canada Validation - MFLP-39
Catalog #
Description
Fraser /Broth
Ready to use
355-5797 225 ml x 6 bottles
355-5794 3 L x 4 bags
355-5792 5 L x 2 bags
Dehydrated Base
356-4604
500 g
Sample
Supplement
356-4616
10 vials
Listeria Special Broth (LSB)

30C
23 hrs 1 hr (1)
or 25 hrs 1 hr (2)

((1) Standard or (2) Easy)
Real-time PCR amplification and detection with

iQ-Check Listeria spp.
iQ-Check Listeria spp. Kit

357-8113
96 tests
LSB (Listeria Special Broth)
Ready to use
355-5703 225 ml x 6 bottles
355-5793 5 L x 2 bags
Dehydrated
356-4703 500 g
356-4753 5 kg
RAPIDListeria spp./Agar
Ready to use
356-3950
20 x 90 mm dishes
Dehydrated Base
356-4744 500 g
Supplement 1
356-4745 10 vials
Supplement 2
356-4746
+/Confirmation of positive with RAPIDListeria spp. or RAPIDL.mono

* Please refer to AOAC and Health Canada certificates for validated matrices
10 vials
RAPIDL.mono/Agar
Dehydrated Base
356-4293
500 g
Supplement 1
356-4294
10 vials (1 vial qsp 500 ml)
Supplement 2
356-4746
For more information on Bio-Rads real-time PCR kits and
instruments, please contact your local Bio-Rad sales representative.
*F
or AOAC-RI protocol, please contact your local Bio-Rad sales
representative.
22 |
02/01/14 16:08
Pathogen Testing

RAPIDE.coli O157:H7 Agar
Detection of Escherichia coli O157:H7
N BRD 07/14-09/07
RAPIDE.coli O157:H7
E. coli O157:H7 Other E. coli
Catalog #
Description

Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Dehydrated
356-4684
500 g
iQ-Check E.coli O157:H7 Kit

357-8114
96 tests
iQ-Check STEC SerO

357-8140
32 tests
Sample
mTSB + Novobiocin
41C
16-24 hrs
Immuno-magnetic-separation
iQ-Check STEC VirX

357-8139
96 tests
mTSB/Broth
Ready to use
(containing novobiocin)
355-5426
225 ml x 6 bottles
Base
356-4426
Isolation on RAPIDE.coli O157:H7
500 g
Dehydrated
356-4610
1g
RAPIDE.coli O157:H7/Agar
Base
356-4748
100 g
37C
24 hrs 2 hrs

Standard tests or Latex or
iQ-Check E.coli O157:H7
SEB (STEC Enrichment Broth)/Broth

356-4001
500 g
356-4002
5 kg
For more information on Bio-Rads real-time PCR kits and
instruments, please contact your local Bio-Rad sales representative.
RAPIDE.coli O157:H7 can also be used as a second medium in the ISO 16654 standard
*F
or AOAC-RI protocol, please contact your local Bio-Rad sales
representative.
| 23
02/01/14 16:08
STEC
iQ-Check STEC VirX and SerO
real-time PCR Kits

iQ-Check E.coli O157:H7 real-time PCR Kit
Detection and Isolation

of non-O157 Shiga Toxin-Producing
Escherichia coli (STEC)
from ground beef
Detection of Escherichia coli O157:H7

NF VALIDATION according to ISO 16140 N BRD 07/15-06/08
Raw beef
Raw ground beef, fresh spinash, apple cider
USDA No Objection Letter
Sample
Sample
SEB enrichment
41.5C
10-15 hrs
41.5C
8-24 hrs

Bacterial Lysis &
Easy DNA Extraction
detection with
iQ-Check STEC VirX
(Automatic results analysis and
interpretation)

with iQ-Check E.coli O157:H7

detection with
iQ-Check STEC SerO
(Automatic results analysis and
interpretation)
Result analysis and interpretation
+/+/Confirmation of posiitve with RAPIDE.coli O157:H7
24 |
02/01/14 16:08
Pathogen Testing
Campylobacter spp. - ISO standard

ISO 10272-1:2006
ISO/TS 10272-2:2006

Horizontal method for detection and enumeration
of Campylobacter spp.

Horizontal method for detection and enumeration
of Campylobacter spp.
Part 1: Detection method
Part 2: Enumeration method
Sample
Sample
Dilution
Bolton Broth
Isolation 0.1 ml
mCDD (x2)
(microaerobic
37C
+ 41.5C
44 4 hrs atmosphere)
4-6 hrs
41.5C
40-48 hrs
Innoculate a steril loop
(microaerobic
atmosphere)
Select 5 colonies
mCDD Agar
Other solid
selective medium
Isolation Columbia blood Agar
(microaerobic
41.5C
41.5C
24-48 hrs
Reading and confirmation

Isolation Columbia blood Agar
41.5C
24-48 hrs
Examination of morphology and motility
Reading and confirmation
Examination of morphology and motility

Study of growth
Columbia blood Agar
(microaerobic
25C
Study of growth
Columbia blood Agar
41.5C
44 4 hrs
Study of growth
Columbia blood Agar
(microaerobic
25C
Study of growth
Columbia blood Agar
41.5C
44 4 hrs
(aerobic
atmosphere)
Oxidase
(aerobic
atmosphere)
Oxidase
| 25
02/01/14 16:08
FDA-BAM Campylobacter spp. protocol

Most samples
(except: Shellfish, milk, frozen dairy products)
25 g sample (50 g vegetables)
100 ml Bolton Broth with antibiotics in net-lined bag

iQ-Check Campylobacter spp. real-time PCR Kit
Detection of Campylobacter jejuni, Campylobacter coli
and Campylobacter lari
For chicken carcass rinses, turkey swabs
and raw ground chicken
Food Sample
Suppl. Bolton Broth
Rinse gently shaking 5 min. (25 rpm on bench top shaker)

Remove net-lined bag
37C + 41.5C
20 hrs
4 hrs
Carcass rinse or sponge

2X BF-BEB
42C
24 hrs
Bacterial Lysis and DNA Extraction

(Easy)
Preenrichment
Microaerobic
or
Microaerobic
Except: bubbling at 30C)
37C
4 hrs
Microaerobic

with iQ-Check Campylobacter
37C
4 hrs
Enrichment
42C
20-44 hrs
Result analysis and interpretation
Streak 24 and 48 hrs enrichments to isolation agar
+/Abeyta-Hunt-Bark agar or mCCDA

Incubate
37C or 42C*
24-72 hrs
* in microaerobic
Identification
Purchase of iQ-Check Kits includes an immunity from suit under patents specified in the product insert to
use only the amount purchased solely in food testing, environmental testing, industrial microbiology and also
for the purchasers own internal research. No other patent rights (such as 5 Nuclease Process patent rights)
are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be
obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City,
California 94404, USA.
26 |
02/01/14 16:08
Pathogen Testing
Campylobacter spp. - Alternative method: RAPIDCampylobacter spp.

Detection of
Campylobacter spp.
Enumeration of
Campylobacter spp.
ISO 16140 (pending)
RAPIDCampylobacter
Campylobacter spp.
Sample enrichment
Suspension/Dilution
Dehydrated
356-4684
500 g
41.5 C 1C
24 hrs 2 hrs
Isolation on
RAPIDCampylobacter
(microaerobic
atmosphere)
(microaerobic
atmosphere)
41.5 C 1C
24-48 hrs

Campylobacter Confirm Latex
or
iQ-Check Campylobacter
or
Standard tests
Description

Ready to use
355-4179
225 ml x 6 bottles
355-5795
3 L x 4 bags
355-5790
5 L x 2 bags
Preston Broth
Isolation on
RAPIDCampylobacter
Catalog #
41.5 C 1C
24-48 hrs

Campylobacter Confirm Latex
or
iQ-Check Campylobacter
or
Standard tests
Confirmation
356-3784 Columbia + Sheep
Blood/Agar,
90 mm x 20 dishes
355-4164 Physiologic Sterile Water
(0.85%), 9 ml x 25 tubes
355-3834 Oxidase test,
2 x 50 disks
355-4154 Sterile Distilled Water,
9 ml x 25 tubes
356-4297
Campylobacter Confirm
Latex kit, 50 tests
iQ-Check Campylobacter/Kit
357-8135 96 tests
RAPIDCampylobacter/Agar
Dehydrated Base
356-4295
500 g
Supplement
356-4296
Tryptone Salt/Diluent
Ready to use
355-5756
90 ml x 6 bottles
355-5754
9 ml x 25 tubes
355-5747
9 ml x 100 tubes
355-5796
3 L x 4 bags
Dehydrated
356-4544
500 g
| 27
02/01/14 16:08
Staphylococcus aureus - ISO standards

NF EN ISO 6888-1/A1 (01/2004)
NF EN ISO 6888-2/A1 (12/2003)
NF EN ISO 6888-3 (06/2003)
Horizontal method for the

enumeration of coagulase-positive
Staphylococci (Staphylococcus
aureus and other species)

enumeration of coagulase-positive
aureus and species)

enumeration wof coagulase-positive
aureus and species)
Technique including confirmation

of colonies
Technique using Rabbit plasma

Fibrinogen agar medium
Detection and MPN technique

for low numbers
and following dilution
and following dilution
Giolitti and Cantoni Giolitti and Cantoni
Broth
Broth
(or)*
Simple concentration Double concentration
Baird-Parker
37C
24 hrs 2 hrs
(+24 hrs 2 hrs)*
Baird-Parker
for RPF
+ RPF sup.
(2 X)*
35C or 37C
24 hrs 2 hrs
(+ 24 hrs 2 hrs)**
(2 X)*
Baird-Parker
Enumeration
35C or 37C
18-24 hrs
and 48 hrs
Confirmation
Brain-Heart Infusion
Direct enumeration of typical

coagulase-positive staphylococci
35C or 37C
24 hrs 2 hrs
Confirmation of
typical colonies
on BHI
Direct
enumeration
of typical
coagulasepositive colonies
Rabbit Plasma
Typical Staphylococci
Grey to black colonies
with a clear halo around
and a white opaque zone
28 |
37C
24-48 hrs
37C
24 hrs
Coagulation reading

** If low or absent colony growth observed
after 24 hours.
Baird-Parker
for RPF
+ RPF sup.
37C
24-48 hrs
Rabbit Plasma
35C or 37C
4-6 hrs or 24 hrs
or
37C
4-6 hrs
or 24 hrs
Reading

* If low or absent colony growth observed
after 24 hours.
02/01/14 16:08
Pathogen Testing
Staphylococcus aureus
Alternative method:
RAPIDStaph Agar
FDA BAM
Sample
Dilutions
Spread 1 ml to 3 plates
Baird Parker
Enumeration of Coagulase-positive
Staphylococci
N BRD 07/9-02/05
All food stuffs, feed and environment
Catalog #
Description
Additives
Egg yolk with potassium tellurite
355-4201
5 ml x 1 vial
355-4205
25 ml x 1 bottle
Sulfamethazine solution (0.2%)
356-2682
2.5 ml x 1 vial
35C
45-48 hrs
Baird-Parker/Agar
Complete medium
356-3991
90 mm x 20 dishes
Base
356-4814
Confirmations
RAPIDStaph
37C
24 hrs 2 hrs
Enumeration and Confirmation
500 g
Baird-Parker for RPF/Agar

Complete medium
357-8618
90 ml x 6 bottles

+ 6 vials of suppl.
356-3996
90 mm x 20 dishes
Dehydrated Base
356-4814
500 g
RPF Supplement
356-4618
10 vials
Pastorex
Staph Plus
or
Spot on
Baird-Parker
for RPF
BHI (Brain-Heart Infusion)/Broth

Ready to use
355-3664
10 ml x 25 tubes
Dehydrated
356-4014
500 g
Pastorex Staph Plus Latex

355-6356
50 tests
355-6353
5 x 50 tests
Rabbit Plasma
Freeze-dried
355-6352
Pack for 20 reactions
Special broth
355-3544
* For AOAC-RI protocol, please contact
your local Bio-Rad sales representative
validated matrices
9 ml x 25 tubes
RAPIDStaph/Agar
Ready to use
356-3960
90 mm x 20 dishes
Base
356-4704
500 g
| 29
02/01/14 16:08
Sulfite-reducing bacteria
TSC
Clostridium perfringens
ISO 15213 (05/2003)
NF EN ISO 7937 (02/2005)

feeding stuffs
enumeration of sulfite-reducing
bacteria growing under anaerobic
conditions
Horizontal method for

the enumeration of
Food sample/initial suspension
Colony-count technique
Heat
treatment
20 min 75C
TSC
(add D-Cycloserine)
Dilution
(2 X)*
Catalog #
TSC
Anaerobes
condition
Description
Lactose Sulfite/Broth
Base
356-4914
100 g
Sheep Blood/Agar
Ready to use
356-3784
90 mm x 20 dishes
Dehydrated Base
356-4674
500 g
(2 X)*
Thermophilous
bacteria
suspicion
50C 24-48 hrs
37C
24-48 hrs
anaerobic
condition
Dehydrated
356-4084
30 |
37C
18-24 hrs
Anaerobes
condition
Lactose
Sulfite
Confirmation
Mobility
Nitrate
46C
24 hrs
Clostridium
perfringens
(black
precipitate/
gas+)
500 g
TSC without D-Cycloserine/Agar Base Medium

Ready to use
355-4419
100 ml x 6 bottles
Base
356-9644
Enumeration
of typical black colonies
Confirmation
of 5 isolated colonies with
Thioglycolate +Resazurin
Reading
Sheep Blood Supplement

355-6652
50 ml vial
Thioglycolate + Resazurin/Broth
Ready to use
356-3613
10 ml x 25 tubes
37C
20 hrs 2 hrs
Clostridium
perfringens
(red
coloration/
gas+)
Lactose
Gelatine
37C
18-24 hrs
Clostridium
perfringens
(yellow/
gas+)
500 g
02/01/14 16:08
Pathogen Testing
Bacillus cereus
Shigella
NF EN ISO 7932 (07/2005)
NFEN ISO 21567 (03/2005)
General guidance for the

enumeration of Bacillus cereus
Horizontal method
for the detection of Shigella spp.
Colony-count technique at 30C
Mossel (MYP)
Mossel (MYP)
Shigella Broth + Novobiocin

PH 7 0.2
41C
16-20 hrs
(2 X)*
Plating-out
30C
18-24 hrs
(+24 hrs)
Confirmation test
on 5 well isolated colonies
Catalog #
XLD
Hektoen
30C
24 hrs 2 hrs
Reading and interpretation
37C
20-24 hrs
(+48 hrs)*
Spot on typical colonies on

Nutrient (2.1%)
37C
20-24 hrs
Biochemical and serological

confirmation
* If low or absent colony growth observed

after 24 hours.
500 g
Mac Conkey + Crystal Violet/Agar

Ready to use
356-3617
90 mm x 20 dishes
Dehydrated
356-9084
Sheep Blood/Agar
Description
Hektoen/Agar
Ready to use
355-4386
100 ml x 6 bottles
356-3894
90 mm x 20 dishes
Dehydrated
356-4284
Mac
Conkey
Bacillus cereus
500 g
Dehydrated
356-4610
1g
Dehydrated
356-4485
500 g
Mossel/Agar
(Mannitol Egg Yolk Polymixin)
Dehydrated
356-9604
500 g
XLD (Xylose Lysine
Desoxycholate)/Agar
Ready to use
354-1751
90 mm x 20 dishes
Dehydrated
356-9124
500 g
| 31
02/01/14 16:08
Validations
PCR Methods
iQ-Check Solutions
AOAC
NF
Validation
Health
Canada
Chromogenic Methods
RAPID Solutions
NordVal
AOAC
NF
Validation
Health
Canada
NordVal
Campylobacter spp.
(pending)
Coliforms
(pending)
Cronobacter spp.
Escherichia coli
Escherichia coli O157:H7
Enterobacteriaceae
Listeria
monocytogenes
Detection
Enumeration
Listeria spp.
Salmonella Enteritidis
Salmonella spp.
Salmonella Typhimurium
STEC 7 major O-groups*
STEC virulence genes*
* No objection letter delivered from USDA
32 |
02/01/14 16:08
Bio-Rad Solutions
Range for Food Testing

Solution Segment
Microorganism
Meat
& Poultry &
Fish Products
Water
& Wine
Beverages
Dairy
Products
Environment
Processed
Foods
Vegetables
Produce
Bacillus cereus
Campylobacter
Coliforms
Cronobacter sakazakii
Enterobacteriacea
Escherichia coli
Escherichia coli O157:H7
STEC
Lactobacillus
Listeria spp.
Listeria monocytogenes
Pseudomonas aeruginosa
Salmonella spp.
Salmonella Enteritidis
Salmonella Typhimurium
Staphyloccocus aureus
Yeasts and Molds

RAPIDChromogenic method
iQ-Check PCR method
Classical media
| 33
02/01/14 16:08
34 |
02/01/14 16:08
Real-Time PCR Methods Food Testing
Start, Run, Results

iQ-Check Prep Solution, when the power
of automation meets real-time PCR
Reliable walk-away automation for real-time PCR testing
Designed to fit lab workflow without disrupting it
Increased confidence with improved reproducibility
Optimized to handle all major food pathogens
For more information: www.foodscience.bio-rad.com
iQ-Check Prep, CFX96 Deep Well and iQ-Check Kits
The Bio-Rad PCR Solution, Your Solution
02/01/14 16:08
Bio-Rad, S.N.C. au capital de 50 000 000 Euros, Locataire-Grant, 449 990 712 RCS Nanterre - N Intracommunautaire FR 62 449 990 712 - Siret 449 990 712 00019
Bio-Rad
Laboratories, Inc.
Life Science
Group
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 31 3689 6600
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Malaysia 60 3 2117 5260 Mexico 52 555 488 7670
The Netherlands 0318 540666 New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700
Russia 7 495 721 14 04 Singapore 65 6415 3170 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700
Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 Thailand 66 2 6518311 United Kingdom 020 8328 2000
Printed in France with soy-based inks on paper from sustainably managed forests
Bio-Rad 25 17933 Rev H 12/2013
02/01/14 16:08

Food Testing

Hochgeladen von

Dokumentinformationen

Copyright

Verfügbare Formate

Dieses Dokument teilen

Dokument teilen oder einbetten

Freigabeoptionen

Stufen Sie dieses Dokument als nützlich ein?

Sind diese Inhalte unangemessen?

Copyright:

Verfügbare Formate

Food Testing

Hochgeladen von

Copyright:

Verfügbare Formate

Food Testing

Food Safety and Quality Diagnostics

17933 - Rev H - User Guide Food Safety V10.indd 1

Coliforms - ISO standards

Coliforms - Alternative method: RAPIDE.coli 2 Agar

Escherichia coli - ISO standards

Escherichia coli - Alternative method:

Enterobacteriaceae - Alternative method:

PART 2 Pathogen Testing

Listeria spp. - Alternative method:

Escherichia coli O157:H7 - Alternative method:

STEC - iQ-Check STEC VirX and SerO

FDA-BAM Salmonella protocol 11

Salmonella - Alternative method:

Escherichia coli O157:H7 - Alternative method:

Campylobacter spp. - ISO standard

Cronobacter spp. - ISO standard

FDA-BAM Campylobacter spp. protocol

Campylobacter spp. - Alternative method:

Campylobacter spp. - Alternative method:

Staphylococcus aureus - ISO standards

FDA-BAM Staphylococcus aureus 29

FDA-BAM Cronobacter spp.

FDA-BAM Listeria monocytogenes protocol 18

Listeria monocytogenes - Alternative method:

Staphylococcus aureus - Alternative method:

17933 - Rev H - User Guide Food Safety V10.indd 2

Routine Testing Quality Indicator

NF EN ISO 6887-1 (09/1999)

NF EN ISO 4833 (05/2003)

Preparation of test samples, initial

General guidance for the

Part 1: General rules for

Colony-count technique at 30C

Buffered Peptone Water/Broth

PCA (Plate Count Agar)/Agar

(and following dilutions)

Food Safety and Quality Diagnostics Bio-Rad Solutions |

17933 - Rev H - User Guide Food Safety V10.indd 3

Escherichia coli - ISO standards

Buffered Peptone Water/Broth

ISO 16649-2 (04/2001)

ISO 16649-1 (04/2001)

MMGA with membrane

Tryptose Lauryl Sulfate/Broth

| Food Safety and Quality Diagnostics Bio-Rad Solutions

17933 - Rev H - User Guide Food Safety V10.indd 4

Routine Testing Quality Indicator

ISO 7251 (07/2005)

Horizontal method for the enumeration

General guidance for detection

Part 3: Most probable number

Enumeration of Escherichia coli

Subculture each tube presenting

Each tube showing the presence of

Indole test reading and calculation

* For detection method only

* Enumeration of typical colonies for E. coli

Food Safety and Quality Diagnostics Bio-Rad Solutions |

17933 - Rev H - User Guide Food Safety V10.indd 5

Coliforms - ISO standards

BGBLB (Brilliant Green Bile

Buffered Peptone Water/Broth

Tryptose Lauryl Sulfate

* Enumeration of typical colonies for E. coli

* Enumeration of typical colonies for E. coli

* Suitable with an automatic plate counter

* Please refer to AOAC certificate for