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In a clinical setting a lot can be required of attending nurses and medical assistants.
During a shift meds are passed, vitals are taken, and tests are ordered, all of which benefit the
patient's health. One of the best diagnostic tools is to have blood drawn from a patient, also
known as 'having labs ordered.' The blood can be viewed as concordance of the body, and
through blood a primary care provider can research and study different factors affecting the body.
To obtain blood is the first step in the process of studying a sample. In the following document
the steps in preparation, the drawing of the blood, the disposal of tools, and the proper way to
It is important to review some basic terminology and physiology of the blood system
before beginning with any procedure. As a technician using phlebotomy in the work force the
first time drawing blood can be a bit nerve wrecking to some, and cause some basic steps and
skills to be forgotten. This is completely understandable, but must be overcome in a short amount
of time to begin giving a high level of care and treatment for patients.
Each patient’s diagnosis will almost always be based upon their lab results. Lab results
give clarity due to the fact that so much of the body is made up of blood. The average person has
about 5 l of blood in them. The blood is made up of two components, plasma which is a liquid
derived from the intestines and organs of the body, and the cells that make up the solids in the
blood are originally formed by bone marrow. ( page 17)The cell part of the blood can be divided
into three main group, erythrocytes, leukocytes, and platelets or thrombocytes. Erythrocytes are
commonly known as red blood cells and leukocytes are commonly known as white blood cells.
The white blood cells can be further divided into two main subgroups, Granulocytes, and Non
granulocytes. (page 17) The granulocytes are made up of Neutrophils, Eosinophils, and
Basophils. The non granulocytes are made up of Lymphocytes and Monocytes.(page 17) All of
these groupings of the different types of cells in the body are important to know during the
diagnosis of blood samples. Each of these components of blood can undergo changes leading to
positive or negative results on the body that could potentially cause severe damage to organs and
The damages to the body are a result of some disorder in a component of the blood.
Frances Fischbach in her manual of laboratory tests states the disorders of red blood cells,
leukocytes disorders, and platelet disorders as an introduction to the importance of the different
parts of blood needing to be in the right range of quantity. “With red blood cells a patient can
suffer from anemia which is a severe reduction in the circulation of red blood cells or
polycythemia which is an abnormal increase in the red blood cell count. The white blood cells
can suffer from a increase number of leukocytes called leuokocytosis or a decrease in the number
hemorrhaging which ultimately is a major cause of a stroke. The other example of platelet count
number of platelets. This can cause an abnormality in the way the blood clots which could lead
The primary way to obtain blood from an individual is through venipuncture. The blood
is acquired from the preferred site of the antecubital vein. During your preparation of drawing
blood you will need to decide what size needle if most suitable for your patient. Fischbach states,
“The size of a needle and syringe is determined by the amount of blood needed as well as the
size and integrity of the vein being used. Furthermore, many hospitals use the Vacutainer system
because it is cheaper. The Vacutainer System consists of a vacuum tube (Vacutainer tube), a
holder, and a multi sample collecting needle.” After you have chosen the proper needle you will
need to gather the vacutainer tubes with the correct colored tops. The colors of the test tubes are
correlated with the test being ordered. For example, if a primary care provider asks a technician
to obtain a CBC with DIFF, then the proper colored top would be lavender. A two columned table
will be documented later in the instructions listing the tube tops matched with the tests that can
As a technician in the form of a nurse or medical assistant one must remember that
drawing labs from a patient is to be done carefully. In Fishbach’s manual, the author gives a
clinical alert to possible problems that may arise if the venipuncture is done incorrectly. Please
review the following outlined information from Frances Fischbach’s manual of laboratory and
diagnostic tests :
• If oozing from the puncture site is difficult to stop, elevate the area and apply a pressure
• Never draw blood for any laboratory test from the same extremity that is being used for
• In patients with leukemia or agranulocytosis and in others with lowered resistance, the
finger stick and earlobe puncture are more likely to cause infection and bleeding than
venipuncture. If a capillary sample is necessary in the patients, the cleansing agent should remain
in contact with the skin for at least 7 to 10 minutes. Alcohol is bactericidal; povodoneiodine is
• Applying sufficient pressure over the puncture site after completion of the procedure
With the understanding of the information listed above, and the general overview of
blood physiology the instructions can now be followed with a better understanding. The blood’s
components are diminutive yet must be balanced properly for the overall health and well being
of a patient. Medical technicians are responsible for making effective decisions towards how to
draw a patient’s labs, what tools will be needed, and how to properly treat the area of the
venipuncture. Keeping all these items in the foreground of one’s mind, the following set of
instructions, tables, and pictures will be easy to follow and helpful in one’s medical career and
treatment of patients.