Beruflich Dokumente
Kultur Dokumente
C6H12O6
+ 6O2
6CO2 + 6H2O
Glucose Metabolism
Glucose
Storage
Glycolysis: oxidation to 3-carbon compounds to provide ATP and metabolic
intermediates
Pentose phosphate pathway - oxidation to yield ribose-5-phosphate and
NADPH
Synthesis of glucose
Gluconeogenesis
Photosynthesis
Glycolysis
- A series of enzyme-catalyzed reactions in a pathway
- Overall conversion:
- These reactions are the way in which carbohydrate is broken down into
smaller units.
- Anaerobic metabolism: pyruvate is converted to lactate or ethanol
(fermentation)
- Aerobic metabolism: pyruvate is fed into the TCA cycle where
it is fully metabolized to carbon dioxide releasing more ATPs
Hexose stage:
(Preparatory phase)
Triose stage:
(Payoff phase)
Hexose stage: 1 to 4
Triose stage: 5 to 10
Hexokinases
- Hexose kinases
- Four isoenzymes isolated from mammalian liver
- Hexokinase I, II, III: Km for glucose = 10-6 10-4 M
- Hexokinase IV = Glucokinase: Km for glucose = 10-2 M
(more active at high blood glucose conc.)
PFK-1
(-D-fructofuranose form)
- The first committed step in glycolysis
Glucose
Glucose 6-P
other pathways
Fructose 6-P
PFK-1
other pathways
other pathways
Fructose 1,6-bisP
Glycolysis only
Aldolases
-
Fate of carbon atoms from the hexose stage to the triose stage:
Arsenate poisoning
- AsO43- replaces inorganic phosphate (PO43-) in phosphoryl transfer
reactions
Glyceraldehyde 3-P
+
AsO43+
NAD+
Glyceraldehyde 3-P
dehydrogenase
(No 1,3-pisphosphoglycerate
production)
2
3
Phosphoglycerate mutase
2
3
(PEP)
(enol form)
(keto form)
Regulation of glycolysis
1. Regulation of hexose transporters
- Membrane-embedded transporters (e.g. GLUT family transporters)
- Stimulation of cellular glucose uptake by insulin
(a) Hexokinase
Glucokinase
- Not inhibited by G-6-P
- the major hexokinase in liver
- Converts glucose to G-6-P in liver after a meal
- G-6-P is used for glycogen synthesis when glucose is sufficient in other tissues
- High Km value for glucose
- Never saturated with glucose
- Activity increases with increasing concentration of available glucose
Glucagon
(Phosphofructokinase-2)
Pyruvate + ATP
fructose 6-phosphate
PFK-1
(not in
some
bacteria)
fructose 1,6bisphosphate