Beruflich Dokumente
Kultur Dokumente
Diameter of Zone of
Inhibition (mm)
Resistan Intermedi Sensiti
t
ate
ve
Antibiotic
Symb
ol
Disc
Concentra
tion
Streptomycin
10 g
11
12-14
15
Tetracycline
Te
30 g
14
15-18
19
Nalidixic Acid
NA
30 g
13
14-18
19
Chloramphenicol
30 g
12
13-17
18
Rifampin
RA
5 g
16
17-19
20
Ampicillin
AM
10 g
13
14-16
17
Ceftriaxone
CRO
30 g
19
20-22
23
Cefuroxime
CXM
30 g
15
15-17
18
Ciprofloxacin
CIP
5 g
15
16-20
21
Doxycycline
DO
30 g
12
13-15
16
Erythromycin
15 g
<13
14-22
>23
Gentamicin
CM
10 g
12
13-14
15
Oxacillin
OX
1 g
<10
11-12
>13
Penicillin
10 g
Staphylococcus
28
29
Enterococcus
14
15
Streptococcus
19
20-27
28
Others
19
20
10
11-15
16
Trimethoprin/Sulfamethox
azole
SXT
25 g
NAME
S:
DATE
PERFORMED:
DATE
SUBMITTED:
used!)
* Pictures/Figures should be presented as appendix/ces.
Sample Table:
Gram Negative
Chemotherape
utic Agent
Escherichia coli
Average
zone size
(mm)
Pseudomonas aeruginosa
Susceptibilit
y
Average
zone size
(mm)
Susceptibilit
y
Ampicillin (AM)
Ceftriaxone
(CRO)
Cefuroxime
(CXM)
Ciprofloxacin
(CIP)
Doxycycline
(DO)
Erythromycin
(E)
Gentamicin (CN)
Oxacillin (OX)
Penicillin G (P)
Trimethoprin/
Sulfamethoxazo
le (SXT)
Gram Positive
Chemotherape
utic Agent
Ampicillin (AM)
Staphylococcus aureus
Average
zone size
(mm)
Susceptibilit
y
Bacillus subtilis
Average
zone size
(mm)
Susceptibilit
y
Ceftriaxone
(CRO)
Cefuroxime
(CXM)
Ciprofloxacin
(CIP)
Doxycycline
(DO)
Erythromycin
(E)
Gentamicin (CN)
Oxacillin (OX)
Penicillin G (P)
Trimethoprin/
Sulfamethoxazo
le (SXT)
Chemotherapeuti
c Agent
Susceptibility
Ampicillin (AM)
Ceftriaxone (CRO)
Cefuroxime (CXM)
Ciprofloxacin (CIP)
Doxycycline (DO)
Erythromycin (E)
Gentamicin (CN)
Oxacillin (OX)
Penicillin G (P)
Trimethoprin/
Sulfamethoxazole
(SXT)
DISCUSSION
State your interpretation of results. End the discussion by summarizing major
conclusions.
Study Questions:
Short but concise answers must be placed after every question.
Cite your references.
1. What factors affect the zone of inhibition?
2. What is the action of each chemotherapeutic agent? Which chemotherapeutic
agent is the most effective? The least effective? (to each test organism)
3. In which growth phase is the organism most sensitive to the antibiotic?
4. What are other methods of measuring the sensitivity of organisms to antibiotic?
5. What is MIC and MBC? How are they measured?
6. What is being measured in the agar-disk diffusion method, bactericidal or
bacteriostatic activity of the organism?
7. Why is the agar-disk diffusion method not a perfect indication on how well the
chemotherapeutic agent will perform in vivo?
8. What is McFarlands Standards? How is it useful to antibiotic sensitivity test?
9. Why is Mueller Hinton Agar used in this test and not other ordinary agar media?