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dna
An NCBE / Unilever educational guide
to the reader
Work with DNA is central to many, if not most, developments
in modern biotechnology. There is growing public awareness
of DNA technologies, their possible applications and wider
implications. However, much of the essential debate about
current genetics has generated more heat than light.
Fortunately, the basic science upon which DNA technologies
are founded features in nearly every school examination
syllabus in biology or science. This booklet is intended
primarily for post-16 students of biology and their teachers.
The practical exercises described here provide an introduction
to some of the classical techniques of molecular biology in a
form suitable for the school laboratory. For reasons of safety
and expense, some the work in this booklet is not particularly
suited to open-ended practical investigations, but some ideas
that may provide starting points for additional work of your
own are given on pages 3637. More ideas are provided in
other NCBE publications such as: The Lambda Protocol;
acknowledgements
The production of this booklet was very kindly supported
by Unilever plc. The contents are entirely the responsibility
of the NCBE however. Unilevers only conditions in
sponsoring this booklet were that we made the publication
freely available via the Internet, and that we consulted our
colleague, Dr Malcolm Thomas, in the Department of
Education at the University of Aberystwyth. Malcolm made
many helpful comments, for which we are grateful. Marjorie
Smith of Dollar Academy was very supportive too, giving us
tips for making this booklet more palatable to those in
Scotland. The molecular structure data for several of the
figures came from the Protein Data Bank and the Nucleic
Acid Database; accession numbers are given by the figures.
Any errors in this booklet are the authors, of course.
2| ILLUMINATING DNA | Version 1.0 | June 2000
COPYRIGHT
The material in this booklet is copyright.
The author and illustrator, Dean Madden, has asserted his moral
right to be identified as the copyright holder under Section 77 of
the Copyright, Designs and Patents Act, UK (1988).
PHOTOCOPYING
Photocopies of this booklet, or selections from it may be made
for educational use, provided that the copies are distributed freeof-charge or at the cost of reproduction, and the author of the
materials is credited and identified as the copyright holder.
contents
04 DNA: molecule of the century
DNA, genes and chromosomes | The genetic code and protein synthesis | Enzymes, precise
molecular tools | Bacterial transformation | Genetically modified plants and animals | The
polymerase chain reaction | Marker genes and gene regulation | DNA gel electrophoresis
| Gene mapping | DNA sequencing | Using genetic data
14 Safety guidelines
Good microbiological laboratory practice
18 Practical activities
Modelling DNA
Physical and computer models are useful for studying molecular structures
18
Extracting DNA
Simple extraction of DNA from onions or fish sperm
20
DNA quantification
Diphenylamine gives a blue-coloured complex with deoxyribose
that can be used to measure the amount of DNA in a sample
22
24
-galactosidase induction
26
Bacterial transformation
Prove that DNA is the genetic material by transforming bacteria
28
30
32
34