Beruflich Dokumente
Kultur Dokumente
LEE
Research Article
Mi Young KANG1, Kyu Hong HEO2, Joo Hee KIM2, Sun Sik CHO2, Pil Dae SEO2, Cyren Mendoza RICO1,
Sang Chul LEE2,*
1
Department of Food Science & Nutrition, Kyungpook National University, Daegu 702-701 - KOREA
2
Received: 28.01.2010
Abstract: The effects of mixture of rice hull charcoal and pyroligneous acid (MRPA) and mixture of wood charcoal
and pyroligneous acid (MWPA) with reduced rate of chemical fertilizer on the yield, and antioxidant and nutritional
quality of rice were investigated. Results showed that RF (Recommended N-P-K fertilizer application = 110-55-48 kg
ha-1) generally had higher agronomic and yield components than MWPA and MRPA treatments while MWPA was more
effective in improving the yield of rice than MRPA. The total phenolic compounds and 2,2-diphenyl-1-picrylhydrazyl
(DPPH) radical electron-donating ability were improved at higher application rate of MWPA while xanthine oxidase
(XO) inhibition was improved at lower MWPA. In the case of nutritional quality of rice, total amylose content increased
while lipid content decreased at lower MWPA. Fatty acid contents were generally high at the combined treatments of
organic material and chemical fertilizer than at chemical fertilizer alone. Furthermore, the results revealed that MWPA
was more appropriate to use than MRPA in increasing both the antioxidant property and nutritional quality of rice.
Key words: Carbonized rice hull, charcoal, pyroligneous acid, rice yield
Introduction
Environmental pollution and proper disposal of
organic agricultural wastes have prompted studies
on developing technologies that will reduce these
problems. In the process of charcoal making,
for example, emission of large amount of gas to
the atmosphere is avoided by trapping the gas to
make a condensate called pyroligneous acid or
smoke vinegar. Moreover, organic by-products of
agricultural produce that would just otherwise be
wasted are now effectively recycled and used in
* E-mail: leesc@knu.ac.kr
45
Effects of carbonized rice hull and wood charcoal mixed with pyroligneous acid on the yield, and antioxidant and nutritional quality
of rice
MRPA
pH
10.90
9.82
OM (%)
53.94
41.80
0.33
0.25
OC (%)
31.29
24.19
C:N
43.45
53.76
EC (dS m-1)
2.34
1.01
P2O5 (%)
0.17
0.22
K (cmol+ kg-1)
0.65
0.49
Ca (cmol+ kg-1)
5.75
5.02
Mg (cmol+ kg-1)
3.29
2.98
N (%)
Effects of carbonized rice hull and wood charcoal mixed with pyroligneous acid on the yield, and antioxidant and nutritional quality
of rice
Table 2. Effects of different mixtures of organic materials and chemical fertilizer on plant height (PH), tiller
number (TN), and chlorophyll content (CC) at maximum tillering and heading stages of rice.
Maximum tillering stage
Treatment
Heading stage
PH
(cm)
TN
CC
(SPAD unit)
PH
(cm)
TN
CC
(SPAD unit)
50% MWPA+50% RF
42.15 ab
21.8 abc
37.3 ab
99.7 a
14.6 a
33.8 a
75% MWPA+25% RF
39.53 bc
21.3 abc
36.5 bc
96.2 abc
13.1 ab
33.5 a
100% MWPA+25% RF
38.93 bc
26.0 a
35.4 cde
96.8 ab
13.8 ab
32.7 a
100% MWPA
35.93 c
19.9 bc
35.4 cde
93.8 bcd
13.0 ab
28.6 cd
50% MRPA+50%RF
36.12 c
18.5 c
35.4 cde
90.8 d
12.2 ab
33.4 a
35.85 c
18.6 c
34.1 de
91.7 d
12.6 ab
32.3 ab
37.97 bc
19.9 bc
35.9 bcd
91.2 d
12.3 ab
31.1 abc
100% MRPA
37.35 bc
20.0 bc
33.9 e
93.2 bcd
11.6 b
27.8 d
RF
44.30 a
25.0 ab
38.8 a
92.8 cd
12.1 ab
29.7 bcd
100% MWPA = 3,000 kg ha-1, 100% MRPA = 2,400 kg ha-1, RF = 110-55-48 kg ha-1.
All values are means of 30 measurements in 3 replicates.
Same letters are not significantly different at P < 0.05 level by LSD.
Table 3. Effects of different mixtures of organic materials and chemical fertilizer on the yield components of rice.
Panicle length
(cm)
Panicle
number
Ripening ratio
(%)
1000 Grain
weight (g)
Yield
(kg ha-1)
50% MWPA+50% RF
21.2
12.9 ab
78.9 ab
27.2 a
6,240 ab
75% MWPA+25% RF
20.3
13.8 ab
77.0 abc
26.9 a
5,689 bc
100% MWPA+25% RF
20.2
12.3 abc
77.3 ab
26.4 ab
6,021 ab
100% MWPA
20.8
11.5 bcd
72.5 bcd
25.7 ab
5,127 cd
50% MRPA+50%RF
20.6
10.2 cde
74.7 abcd
26.7 ab
4,416 de
21.4
75.5 abcd
26.7 ab
4,759 de
21.1
10.4 cde
74.6 abcd
25.8 ab
4,617 de
100% MRPA
19.8
9.8 de
70.1 d
24.9 b
4,067 e
RF
21.6
14.5 a
70.7 cd
25.7 ab
6,573 a
Treatment
9.0 e
100% MWPA = 3000 kg ha-1, 100% MRPA = 2400 kg ha-1, RF = 110-55-48 kg ha-1.
All values are means of 30 measurements in 3 replicates.
Same letters are not significantly different at P < 0.05 level by LSD.
Table 4. Effects of different mixture organic materials and chemical fertilizer on the total phenolic compound,
DPPH radical scavenging activity, and xanthine oxidase inhibition of brown rice.
Treatment
Xanthine oxidase
inhibition (%)
Vitamin C
91.36 0.03 a
Vitamin E
90.38 0.10 b
Butylated hydroxytoluene
90.55 0.10 ab
100% MWPA
18.78 0.08 b
32.16 1.65 f
14.39 1.54 e
100% MWPA+25% RF
20.02 0.12 a
44.45 0.53 c
14.26 0.24 e
75% MWPA+25% RF
18.27 0.02 f
36.78 1.06 d
11.97 0.52 f
50% MWPA+50% RF
18.57 0.03 e
30.90 0.73 g
44.79 1.47 a
100% MRPA
14.32 0.02 h
14.04 1.81 i
19.78 1.47 d
100% MRPA+25% RF
16.81 0.06 g
30.81 0.64 g
18.86 0.12 d
75% MRPA+25% RF
18.63 0.05 d
28.07 0.29 h
25.10 0.56 c
50% MRPA+50% RF
18.70 0.07 c
31.21 1.19 g
43.31 2.11 b
RF
18.27 0.06 f
34.25 0.98 e
25.59 0.17 c
100% MWPA = 3000 kg ha-1, 100% MRPA = 2400 kg ha-1, RF = 110-55-48 kg ha-1.
All values are means of 3 replicates.
Same letters are not significantly different at P < 0.05 level by LSD.
49
Effects of carbonized rice hull and wood charcoal mixed with pyroligneous acid on the yield, and antioxidant and nutritional quality
of rice
Discussion
The higher agronomic character and yield in RF was
quite expected since RF contained high amounts
of readily available nutrients needed by the plants.
MRPA treatments had a decreasing trend in the values
in panicle number and ripening ratio compared
to those observed in the MWPA treatments. This
trend resulted in lower yields recorded in the MRPA
treatments compared to those obtained in the
MWPA treatments. This could also be attributed to
the generally high nutrient components like organic
matter, organic carbon, nitrogen content, and
exchangeable cations in MWPA than in MRPA.
The higher application rate of MWPA and
lower dose of chemical fertilizer could increase the
antioxidant quality in terms of radical scavenging
activity of rice compared with RF. This observation
was also reported in other studies (Rico et al. 2007;
Kang et al. 2008). The higher antioxidant quality of
rice in MWPA than MRPA could also be attributed
to the higher nitrogen and organic matter contents
of MWPA. However, higher application rate of both
MWPA and MRPA at lower dose of chemical did not
Table 5. Effects of different mixture organic materials and chemical fertilizer on the
starch amylose and lipid contents of brown rice.
Starch amylose
(%)
Lipid content
(mg g-1)
100% MWPA
0.2231 0.01 de
15.18 0.01 a
100% MWPA+25% RF
0.2329 0.01 cd
13.95 0.01 g
75% MWPA+25% RF
0.2061 0.01 f
14.27 0.02 e
50% MWPA+50% RF
0.2773 0.00 a
14.98 0.02 b
100% MRPA
0.2281 0.01 de
14.38 0.20 d
100% MRPA+25% RF
0.2160 0.02 ef
14.27 0.01 e
75% MRPA+25% RF
0.2294 0.01 d
14.14 0.02 f
50% MRPA+50% RF
0.2560 0.00 b
14.39 0.01 c
RF
0.2428 0.00 c
13.16 0.01 h
Treatment
100% MWPA = 3000 kg ha-1, 100% MRPA = 2400 kg ha-1, RF = 110-55-48 kg ha-1.
All values are means of 3 replicates.
Same letters are not significantly different at P < 0.05 level by LSD.
50
0.34 0.01 a
0.29 0.03 c
0.33 0.01 ab
0.29 0.02 c
0.33 0.02 ab
0.32 0.00 b
0.33 0.00 ab
0.32 0.17 b
100% MWPA+25% RF
75% MWPA+25% RF
50% MWPA+50% RF
100% MRPA
100% MRPA+25% RF
75% MRPA+25% RF
50% MRPA+50% RF
RF
17.02 0.44 de
17.46 0.11 cd
17.46 0.57 cd
17.90 0.33 bc
16.84 0.60 e
17.75 0.22 bc
1.28 0.05 ab
1.23 0.06 ab
1.32 0.17 bc
1.20 0.15 ab
1.29 0.02 c
1.33 0.06 a
1.24 0.02 ab
1.23 0.07 ab
1.10 0.11 c
Stearic acid
(C18:0)
Same letters are not significantly different at P < 0.05 level by LSD.
100% MWPA = 3000 kg ha-1, 100% MRPA = 2400 kg ha-1, RF = 110-55-48 kg ha-1.
18.16 0.14 b
0.29 0.02 c
100% MWPA
18.90 1.14 a
Palmitic acid
(C16:0)
Myristic acid
(C14:0)
Treatment
17.39 0.41 ab
17.02 0.44 ab
17.46 0.11 bc
17.46 0.57 ab
17.90 0.33 c
18.84 0.60 a
17.75 0.22 ab
18.90 1.14 a
18.16 0.14 bc
Palmitoleic acid
(C16:1)
35.65 0.24 bc
35.40 0.07 c
34.81 0.30 d
35.42 0.66 bc
34.25 0.21 e
36.46 0.23 a
35.73 0.10 b
35.43 0.20 bc
35.66 0.23 bc
Oleic acid
(C18:1)
44.91 0.04 ab
45.21 0.18 a
43.70 0.76 e
43.76 0.14 de
42.87 1.10 f
43.63 0.09 e
Linoleic acid
(C18:2)
Table 6. Effects of different mixture organic materials and chemical fertilizer on the fatty acid contents (%) of brown rice.
1.05 0.04 c
1.05 0.07 c
1.05 0.08 c
1.06 0.15 c
0.93 0.10 d
1.19 0.05 a
1.09 0.05 bc
1.09 0.14 bc
1.15 0.01 ab
Linolenic acid
(C18:3)
51
Effects of carbonized rice hull and wood charcoal mixed with pyroligneous acid on the yield, and antioxidant and nutritional quality
of rice
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