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Volume 1; Issue - 3; Year 2015; Page: 205 212
Leelevinothan Pari / Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(3): 205 212
206
Induction of Experimental DM
DM was induced in overnight fasted rats by a
single intraperitoneal injection of STZ
(40
mg/kg body weight) dissolved in freshly prepared
citrate buffer (0.1 M, pH 4.5). STZ injected rats
were allowed to drink 20% glucose solution
overnight to overcome the initial drug-induced
hypoglycemic mortality. The induction of DM in
rats was confirmed by estimating the elevated
plasma glucose levels, 72 h after STZ injection.
Rats with fasting plasma glucose levels more than
250 mg/dL were considered diabetic and chosen
for the current study.
Experimental Design
A total of 42 rats (30 STZ-induced diabetic
rats and 12 normal rats) were used and they were
divided into 5 groups with 6 rats in each group as
follows:
Group I : Normal control rats.
Group
II
Group
III
Group
IV
Experimental Animals
Male albino Wistar rats, weighing about 180 220 g were procured from Central Animal House,
Group
Leelevinothan Pari / Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(3): 205 212
Group
VI
Group
VII
207
Leelevinothan Pari / Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(3): 205 212
208
Glucose (mg/dL)
86.70 6.60a
87.38 6.69a
271.39 20.66b
215.39 16.49c
185.85 14.23d
117.92 9.03e
102.61 7.85f
Normal control
Normal +Tyrosol (20 mg/kg bw)
Diabetic control
Diabetic + Tyrosol (5mg/kg bw)
Diabetic + Tyrosol (10mg/kg bw)
Diabetic + Tyrosol (20mg/kg bw)
Diabetic + Glibenclamide (600g/ kg bw)
Insulin (U/ml)
16.09 1.23a
16.34 1.25a
6.21 0.47b
8.23 0.63c
11.10 0.85d
12.93 0.99e
14.70 1.12f
Each value is mean S.D. for six rats in each group. In each group, means with different superscript letter (af) differ significantly
at p< 0.05 (DMRT).
c
a
ac
0
Normal
control
Normal +
Tyrosol
(20 mg/kg)
Diabetic
control
Diabetic +
Tyrosol
(20 mg/kg)
Diabetic +
Glibenclamide
(600 g/kg)
Groups
Each column is mean S.D. for six rats in each group. In each bar, means with different letter (ac) differ significantly at P<0.05
(DMRT)
-5
Plasma LOOH ( x 10 mmoles/dL)
16
14
12
10
c
a
ac
8
6
4
2
0
Normal
control
Normal +
Tyrosol
(20 mg/kg)
Diabetic
control
Diabetic +
Tyrosol
(20 mg/kg)
Diabetic +
Glibenclamide
(600 g/kg)
Groups
Each column is mean S.D. for six rats in each group. In each bar, means with different letter (ac) differ significantly at P<0.05
(DMRT)
Leelevinothan Pari / Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(3): 205 212
209
Vitamin C
Vitamin E
a
2.0
ac
c
a
a
ac
1.5
mg/dL
1.0
b
b
0.5
0.0
Normal
control
Normal +
Tyrosol
(20 mg/kg)
Diabetic
control
Diabetic +
Tyrosol
(20 mg/kg)
Diabetic +
Glibenclamide
(600 g/kg)
Groups
Each column is mean S.D. for six rats in each group. In each bar, means with different letter (ac) differ significantly at P<0.05
(DMRT)
25
a
ac
20
15
10
0
Normal
control
Normal +
Tyrosol
(20 mg/kg)
Diabetic
control
Diabetic +
Tyrosol
(20 mg/kg)
Diabetic +
Glibenclamide
(600 g/kg)
Groups
Each column is mean S.D. for six rats in each group. In each bar, means with different letter (ac) differ significantly at P<0.05
(DMRT)
Leelevinothan Pari / Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(3): 205 212
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Leelevinothan Pari / Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(3): 205 212
ed,
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Leelevinothan Pari / Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(3): 205 212
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