Beruflich Dokumente
Kultur Dokumente
ISSN: 2231-3354
Received on: 09-12-2011
Revised on: 17:12:2011
Accepted on: 20-12-2011
ABSTRACT
Thimmayan Baluprakash,
Karuppannan Arumugasamy,
Subramaniam Paulsamy,
Madathupatti Ramanathan
Udhayasankar and Uthaman Danya.
PG and Research Department of
Botany, Kongunadu arts and science
college (Autonomous), Coimbatore,
Tamilnadu, India.
Keywords: Exacum wightianum, Callus culture, multiple shoot regeneration, BA+ NAA.
INTRODUCTION
For Correspondence
Karuppannan Arumugasamy,
Email:
arumugasamy_kasc@yahoo.co.in
Table 1. Effect of different concentration of growth regulators on callus induction from leaf, node and shoot tip explant of Exacum wightianum
Growth regulators (mg/l)
BA
0.5
1.0
1.5
2.0
2.5
3.0
0.5
1.0
1.5
2.0
2.5
3.0
0.5
1.0
1.5
2.0
2.5
3.0
0.5
1.0
1.5
2.0
2.5
3.0
NAA
0.03
0.03
0.03
0.03
0.03
0.03
-
2,4-D
0.3
0.5
1.0
1.5
2.0
2.5
-
Kn
0.1
0.3
0.5
0.8
1.0
1.5
Node
530.02
570.08
670.03
720.13
780.16
730.06
540.12
590.07
760.11
820.14
690.08
540.02
560.01
720.06
780.06
710.02
680.09
570.08
590.03
520.06
720.02
760.05
670.08
540.05
Axial bud
640.02
670.04
690.08
780.14
810.19
740.05
670.15
720.04
790.14
850.21
670.09
620.04
740.04
780.02
830.07
760.07
640.02
590.04
580.05
620.04
690.02
790.09
610.07
580.04
Node
WF
WF
LGF
GF
GC
GF
GC
WF
LGF
GC
GC
GC
WF
WF
LGF
WF
GF
LGF
WF
GF
LGF
WF
LGF
WF
Axial bud
LGF
LGF
WF
GC
WF
WF
GF
GF
GC
GC
LGF
GF
GC
LGF
GC
GF
WF
WF
WF
GF
GC
LGF
WF
GF
WF- White coloured, fragile. LGF- Light green coloured ,fragile. GC- Green coloured ,compact. GF- Green coloured ,fragile
Shooting
response
(%)
No. of
shoots/callus
Height of
Shootlets (cm)
0.0 0.0
AP
NAA
Kn
0.0 0.0
0.5
480.02
2.8 0.83
0.8 0.1
1.0
600.05
6.8 0.32
3.4 0.14
1.5
720.09
8.2 1.0
5.2 0.8
2.0
760.04
18.2 0.7
9.68 0.5
2.5
840.10
21.5 0.3
10.26 0.3
3.0
690.12
2.6 041
0.7 0.2
0.0 0.0
0.0 0.0
0.5
0.3
390.14
3.4 0.5
0.9 0.2
1.0
0.3
520.08
4.9 0.2
3.8 0.31
1.5
0.3
760.07
7.8 0.6
6.2 0.9
2.0
0.5
880.02
25.1 0.1
14.6 0.4
2.5
0.5
730.06
7.2 0.4
5.4 0.1
3.0
0
0.5
1.0
1.5
0.5
-
0
0.1
0.2
0.3
560.04
0
490.12
580.14
710.08
5.8 0.7
0.0 0.0
2.9 0.6
4.1 0.5
7.9 0.6
3.4 0.8
0.0 0.0
0.6 0.1
2.9 0.4
5.3 0.2
2.0
0.4
780.09
14.25 0.1
9.47 0.3
2.5
0.5
650.04
10.4 0.5
7.9 0.1
3.0
0.6
520.01
8.6 0.2
6.2 1.0