Beruflich Dokumente
Kultur Dokumente
pubs.acs.org/JAFC
Department of Marine Sciences and Convergent Technology and Department of Molecular and Life Science/Bio-nanotechnology,
Hanyang University, Ansan 426-791, Republic of Korea
INTRODUCTION
Fishery products are among the chief internationally traded
food commodities.1 They improve nutrition, health, and wellbeing2 and, are a prime source of -3 fatty acids, vitamins,
minerals, trace elements, high-quality protein, and essential
amino acids. Acceleration and diversication of transport
(especially maritime trac), increased seafood trade and
consumption, and, greater demand for certain types of sh or
seafood have globalized the sheries trade. However,
concomitant uctuations in supply and inability to meet the
phenomenal demand have encouraged fraudulent practices of
substitution. As a huge number of shery species are either
consumed or utilized as raw material for dierent marketed
preparations, authentication (of species and its origin) becomes
quintessential for confronting deceptive practices. Accurate
labeling of shery products would deter commercial fraud and
prevent potential safety hazards caused by the introduction of
food ingredients that might be harmful to human health.
Fishery products represent a signicant market niche: they
include a wide variety of commercially valuable species such as
hairtail sh (belonging to the family Trichiuridae) and shrimp
(belonging to the superfamily Penaeoidae). The hairtail
comprises the sixth most important captured sh species,
with their present world catch exceeding 1.5 million tons
annually.3 On the other hand, shrimp (either harvested by
extractive shing or farmed in aquaculture facilities) account for
more than 30% of the worldwide demand of crustacean
species.4 Traditional identication and authentication of sh
and penaeid shrimps are carried out by examining morphological features (such as body shape, color, size, ns, and other
2015 American Chemical Society
Article
primera
sequence (5 to 3)
target groupb
reaction type
FP-1
RP-1
FP-2
RP-2
FP-3
RP-3
probe 1
probe 2
probe 3
AGAGCTAAGCCAACCAGGCT
AATTATCACGAAGGCATGGG
AGGCACAGCCTTAAGCCTTCT
GCTGTAACGATAACATTGTAAATTTGG
AACTGGCTTATCCCCCTAATGAT
GCGGAGGAGGCTAGGAGAAG
[FAM]- CCCTCCTGGGCGATGACCAA -[TAMRA]
[HEX]- CCGCGCAGAGCTAAGCCAGCC-[TAMRA]
[FAM]- CCGACATGGCCTTCCCCCG -[TAMRA]
G3
G3
G2
G2
G1
G1
G3
G2
G1
multiple
multiple
multiple
multiple
single
single
multiple
multiple
single
FP, forward primer; RP, reverse primer. bGroup 1 (G1): Korea, Japan, and China. Group 2 (G2): Senegal and Canada. Group 3 (G3): Thailand.
COI-V-F
COI-V-R
COI-In-F
COI-In-R
5-TCAACCAACCACAAAGACATTGGCAC-3
5- TAGACTTCTGGGTGGCCAAACAATCA-3
5- GTTCAACAAATCATAAAGATATTGG-3
5- TAAACTTCAGGGTGACCAAAAAATCA-3
DOI: 10.1021/acs.jafc.5b01469
J. Agric. Food Chem. 2015, 63, 55485556
Article
sequence (5 to 3)
target groupb
reaction type
FP-1
RP-1
FP-2
RP-2
probe 1
probe 2
AACTGGCTTATCCCCCTAATGAT
GCGGAGGAGGCTAGGAGAAG
AGGCACAGCCTTAAGCCTTCT
GCTGTAACGATAACATTGTAAATTTGG
[FAM]- CCGACATGGCCTTCCCCCG -[TAMRA]
[HEX]- CCGCGCAGAGCTAAGCCAGCC-[TAMRA]
G1
G1
G2
G2
G1
G2
single
single
multiple
multiple
single
multiple
a
FP, forward primer; RP, reverse primer. bGroup 1 (G1): Korea (farmed), Malaysia, and Ecuador. Group 2 (G2): India, Netherlands, Thailand, and
Indonesia.
Figure 1. Biplots of 13C and 15N values (mean SD) of (a) hairtail
sh and (b) shrimp based on their geographical origin. Grouping was
carried out based on the LDA analysis. Data represent the mean
SD of ve replicates. (a) Group 1 (G1, red circles) represents
samples from three countries: Korea [South Sea ID 102 (1), South
Sea ID 110 (2), South Sea ID 234 (3), Mokpo (MP) (4), and
Gunsan (GS) (5)], China (7), and Japan (8). Group 2 (G2, orange
inverted triangles) represents samples from Canada (6) and Senegal
(9). Group 3 (G3, yellow squares) represents samples from Thailand
(10). (b) Group 1 (G1, red circles) represents wild samples from
Korea [Taean (TA) (6) and Gunsan (GS) (7)]. Group 2 (G2,
orange inverted triangles) represents wild samples from Korea Guje
(GJ) (5). Group 3 (G3, yellow squares) represents wild samples
from Yeosu (YS), Korea (8). Group 4 (G4, green diamonds)
represents aquacultured samples from Korea [Gunsan (GS) (1),
Anmyoendo (AM) (2), Mokpo (MP) (3), and Ansan (AS) (4)] as
well as wild samples from Ecuador (12) and Malaysia (15). Group 5
(G5, light blue triangles) represents wild samples from India (9),
Indonesia (10), Thailand (11), and Netherlands (14). Group 6 (G6,
dark blue hexagons) represents wild samples from Argentina (13).
RESULTS
The mean isotopic values and standard deviation [when the
number of authentic samples (n) was 5] are provided in
Figure 1a. As evidenced in Figure 1a, distinct carbon and
nitrogen isotopic values of hairtail sh samples of various
geographic origins were obtained; that is, the 13C and 15N
() values of the sh diered signicantly based on their
origin.
The 13C values of the ve Korean hairtail sh ranged from
17.16 0.56 to 18.03 0.27, whereas the 13C
values of other, non-Korean hairtail sh were 15.55
0.46 (Canadian), 17.90 0.23 (Chinese), 15.43
0.56 (Japanese), 15.73 0.32 (Senegalese), and
17.80 0.14 (Thai). On the other hand, nitrogen
isotope (15N) values of the Korean hairtail sh ranged from
11.66 0.16 to 13.08 0.65, while the values for
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DOI: 10.1021/acs.jafc.5b01469
J. Agric. Food Chem. 2015, 63, 55485556
Article
Figure 2. Grouping of (a) hairtail sh and (b) shrimp based on LDA of stable isotopes (13C and 15N). Values in parentheses represent percentage
similarity.
DOI: 10.1021/acs.jafc.5b01469
J. Agric. Food Chem. 2015, 63, 55485556
Article
Figure 3. Evolutionary relationships of (a) 10 taxa (linearized) of hairtail sh and (b) 15 taxa (linearized) of shrimp, and neighbor-joining (NJ)
phylogenetic trees inferred from DNA sequences of mitochondrial gene COI.
DISCUSSION
Fishery products are considered as a staple food in Korea. In
fact, the per capita seafood consumption here is considerably
larger than average. In the sh industry, regulations regarding
labeling, product quality, and origin have been tightened.
Therefore, it is mandatory that sh processing rms must
produce proof of origin and traceability. This makes it
absolutely essential to formulate an eective traceability system
to track sh products throughout the supply chain (harvesting,
processing, and marketing). However, identication becomes a
rather daunting task in the case of analogous (closely related)
sh species. Proper identication of hairtail shes and their
origin is relatively dicult due to their morphological similarities. In Japan, consumer preference for Tachiuo and
Tenjikutachi vary with regard to organoleptic characteristics;
however, fresh ones are mainly used for raw consumption
(sashimi).12 In addition, most of the morphological characteristicss that are used for species identication (such as head
pattern, presence or absence of pectoral n, dorsal and anal n
ray counts) are lost upon processing; thus, identication of sh
species in processed llets is extremely dicult. This also holds
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DOI: 10.1021/acs.jafc.5b01469
J. Agric. Food Chem. 2015, 63, 55485556
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DOI: 10.1021/acs.jafc.5b01469
J. Agric. Food Chem. 2015, 63, 55485556
Article
stable isotopic values also vary with the country of origin; for
example, the shrimp P. borealis from Greenland has dierent
13C and 15N values (18.1 and 10.3)34 compared to
shrimp from the Mid-Atlantic Ridge (10.9 and 7) and
Japan (14.7 and 15.8).29,35 Ortea and Gallardo6
reported unique 13C and 15N values of shrimp species from
Argentina (16.12 and 16.86), North Atlantic (18.07
and 10.78), Mozambique (16.77 and 10.55), Nigeria
(17.87 and 10.34), and Senegal (16.80 and
8.72). These reported 13C and 15N values of various
shrimp species from dierent countries diered signicantly
from the values of shrimp obtained in our study. This reveals
that stable isotopic values could positively indicate the origin of
shrimp.
In the present investigation, shrimp from the natural environment showed higher isotopic variability and their mean 13C
and 15N values were statistically dierent than the farmed
ones. The 13C values of Korean aquacultured shrimp ranged
from 20.23 to 21.72, whereas the 13C values of
Korean natural shrimp ranged from 14.02 to 18.69. In
contrast, the 15N values of aquacultured shrimp (5.60
6.20) were lower than the 15N values (11.5312.28) of
natural shrimp; this was probably because of dierences in diet.
Gamboa-Delgado et al.36 also reported distinct 13C and 15N
values for the shrimp species L. vannamei from Ecuador (open
sea 16.6 and 9.76, estuary 18.32 and 4.25, semiintensively farmed 19.86 and 6.02) and L. vannamei
from the Gulf of Mexico (open sea 17.14 and 12.19,
estuary 18.59 and 5.88, semi-intensively farmed
19.69 and 8.32, intensively farmed 21.56 and
8.69). Aquaculture feeds are frequently formulated with
varying dietary levels of plant meals. These plant meals are
mostly derived from terrestrial plants having C3 photosynthesis
and are less enriched in 13C (mean 13CVPDB = 29) as
compared to C4 plants (mean 13CVPDB = 13).36 Also, the
C3 plants are less enriched as compared to several marinederived ingredients such as sh meal (13CVPDB = 17). For
example, some shrimp feeds used in Mexico have 13CVPDB
values ranging from 23.6 to 22.3 and 15N values
ranging from 5.8 to 9.7; the 13CVPDB values of feed are
transferred to the farmed organisms, thereby causing contrasting 13C values (more isotopically depleted) as compared
to the wild type.36 Thus, stable isotopic values could help
distinguish farmed and natural shrimp.
The unique isotopic values obtained for hairtail sh and
shrimp obtained from various regions, viewed through the
present study, assert that 13C and 15N could be used as
discriminatory variables to distinguish between various geographic origins.
Although several schematic reports and strategies have been
outlined to identify shery products, determine their geographical origin, and preserve domestic shery products
without adulteration, most of the techniques involved discuss
animal meat (beef and lamb). But this study brings forth the
applicability of isotopic analysis in the traceability of marine sh
products. The identied dierences in isotopic patterns of
samples from various origins obtained in this study elucidate
the potential of stable isotope analysis for validation of
geographic authenticity. However, the identied patterns will
have to be conrmed in another detailed study comprising a
larger number of samples from certied origins and over a time
course. Nevertheless, it is a fact that isotopic ratios of shes are
bound to be more stable as compared to other smaller life
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DOI: 10.1021/acs.jafc.5b01469
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AUTHOR INFORMATION
Corresponding Author
Funding
REFERENCES
(1) Ortea, I.; Pascoal, A.; Canas, B.; Gallardo, J. M.; BarrosVelazquez, J.; Calo-Mata, P. Food authentication of commercially
relevant shrimp species: From classical methods to foodomics.
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(2) Tacon, A. G. J.; Metian, M. Fish matters: Importance of aquatic
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(3) http://www.dpi.nsw.gov.au/__data/assets/pdf_le/0008/
375902/Hairtail-and-Frostsh.pdf (accessed on July 27, 2014).
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J. M.; Calo-Mata, P. Molecular identification of the black tiger shrimp
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