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3-Tyagi R.K. and Prakash S. 2004. Genotype-and sex-specific protocols for in vitro micropropagation and
medium-term conservation of jojoba. Biologia Plantarum. 48:19-23.
4-Mills D. Yanqing Z. and Benzioni A. 2004. Improvement of jojoba multiplication in vitro by
ventilation. In vitro Cell. Dev. Biol. Plant. 40: 396-402.
5-Saghari, Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard R.W. 1984. Ribosomal DNA spacerlength polymorphism in barley: Mendelian inheritance, choromosomal location, and population dynamics.
Proc Natl Acad Sci USA. 81: 8014-8018.

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The 6th National Biotechnology Congress of Iran

13-15 Aug, 2009, Milad Tower Conference Hall, Tehran-Iran

Determination of suitable method for DNA extraction from leaf tissue of

jojoba shrub
Hosseini, F1*,**. Shahsavand Hassani, H2. Arvin, M. J2,3, Mohammadinejad, Gh2. Baghizade, A3.
1
Agricultural Biotechnology MS.c student of Shahid Bahonar Univercity of Kerman, Kerman, Iran; 2 college of
agriculture, Shahid Bahonar Univercity of Kerman, Kerman, Iran; 3 International Center for Science, High Technology
and Environmental Science, Kerman, Iran

Abstract
Jojoba is an evergreen and dioecious shrub, that commercially valuable for it,s seed. The seed containing a
characteristic liquid wax of economic importance in industry, nutrition and medicine (e.g. cosmetics,
anticancer compounds). In recent years, the breeding and biotechnological programs were expand on this
shrub. The extraction of DNA is the first step for such programs. In this study, the optimum method
investigated for genomic DNA extraction, among of two modified CTAB and dellaporta methodes, ttest to
be shown that suitable method for genomic DNA extraction from jojoba leaf is CTAB.
Key words: CTAB, Jojoba, Dellaporta method, Ttest, DNA extraction