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for vitamin D molecules.3 Around 50% of the previtamin D3 can isomerize to vitamin D3
within 2.5 hours in the skin. Within 1224 hours after UVB exposure, the circulating
concentrations of vitamin D3 are at their maximum levels.3 If previtamin D3 is formed in
the skin, it can also undergo either photoisomerization to lumisterol, tachysterol, and
toxisterols, or it can be retransformed to 7-DHC.3 A broad overview of this process can be
seen in Figure 1.
Figure 1: The subcutaneous synthesis of vitamin D3 with the help of UVB and thermal
energy. Also shows alternate paths available for the compounds used in the synthesis that
may occur in the dermal layer.
A At this point, the vitamin D is biologically inactive and must be activated in the
liver and the kidneys. This is also the form vitamin D comes in through consuming
supplements.The vitamin D found in vitamin supplements is also in this inactive form.
(consumption). After the inactive vitamin D binds to the carrier proteins, vitamin Dbinding protein (DBP), itInactive vitamin D is transported to the liver where it is
enzymatically hydroxylated to 25-hydroxyvitamin D [25(OH)D].5 The hydroxylation into
25-hydroxyvitamin D [25(OH)D] is catalyzed by microsomal cytochrome P450
enzyme CYP2R1 and/or the mitochondrial cytochrome P450 CYP27A1 (see Figure 2),;
both of which are constitutively expressed.3,6 Cytochromes are hemoproteins that supply
ATP via electron transport to the molecules. Besides the CYP2R1 and CYP27A1, there
are also several other cytochrome P450 mixed function oxidases
(CYP2C11,, CYP3A4,, CYP2D25, and CYP2J3) that exhibit vitamin D 25-hydroxylase
activities.3 The normal circulating levels of 25(OH)D in the blood are between 25 nmol/L
and 200 nmol/L.3 Currently, vitamin D levels of about 30 ng/mL
(75 nmol/L) are considered to be optimal for health.
(homeostasis).
25-hydroxyvitamin D [25(OH)D] , bound
to DBP, is then transported to the kidneys and is
finally hydroxylated by CYP27B1 (25hydroxyvitamin D-1-hydroxylase or; 1OHase) at the
C1 position to hormonally active 1,25dihydroxyvitamin D [1,25(OH)2D].3 The overall activation
of vitamin D3 can be seen in Figure 2 where there are two
separate pathways 25(OH)D can take to form two
Figure 2: Pathway from vitamin D to
the active form (calcitriol, bottom) and
/inactive form (right) through the use
ofprocessing by various cytochromes.
Also shows where the cytochromes
Figure2:PathwayfromvitaminDtotheactiveform/inactiveformthroughtheuseofvarious
cytochromes.Alsoshowswherethecytochromestakeaction(liver,kidney)andshowsthechemical
compositionoftheproductofthechemicalpathway.
the GC gene residing on chromosome 4.1 DBP consists of 458 amino acids, including
numerous cysteine residues, which form multiple disulfide bridges within the protein.
The three domains of the protein have many -helices; six of them on the first domain
form the binding site for vitamin D ligands.7
DBP plays an important role in maintaining stable supplies of
vitamin D for the body. When researchers knocked out the gene coding for
DBP in mice, the knockout mice did not seem to have any physiological
defects. However, when fed a vitamin D deficient diet, the knockout mice
showed signs of bone disease (a common symptom of vitamin D deficiency)
sooner than the wild-type mice, suggesting that they were less able to cope
with vitamin D depletion.1 Another study showed that the kidneys recover vitamin-D
bound DBP from urine, demonstrating the mechanism by which DBP aids retention of
vitamin D.1 7
Cells in the body have several ways of accessing the bodys supply of vitamin D
Figure 42: Binding of 1,25D to CYP24A1 located in the
inner mitochondrial membrane. The heme group (red dotted
sphere) reduces oxygen to hydroxylate 1,25D
vitamin D across the cell membrane. The level of free vitamin D in the body is governed
by the levels and affinity of DBP. Vitamin D can also enter the cell while still bound to
DBP through active-receptor-mediated uptake. The DBP-vitamin D complex binds to a
cell surface receptor called megalin (LRP2) and is internalized in a vesicle, where
vitamin D is released and DBP is denatured.71 Once inside cells, 1,25(OH)2D1,25D
directs vitamin D-dependent gene regulation through the vitamin D receptor (VDR),
while 25(OH)D is first converted into active 1,25(OH)2D 1,25D through hydroxylation of
the 1- carbon by cytochrome p450 27B1 (CYP27B1, also known as 1- hydroxylase),
usually located in the inner mitochondrial membrane.1,27,8 This oxidation reaction of
25(OH)D occurs when NADPH-CYP reductase captures an electron pair from the
conversion of NADPH to NADP and transfers it to CYP27B1, which has high specificity
for 25-hydroxylated steroids (i.e. 25(OH)D) and reduces oxygen via the heme group in its
active site to hydroxylate 25(OH)D (see Figure 2).2,36,8 The mechanism is likely similar to
the reduction of oxygen to water,
which involves heme D, the site of
oxygen reduction in many types of
bacteria.20 Another cytochrome,
CYP24A1, operates in a similar
fashion to catalyze the hydroxylation of
Figure 42: Binding of 1,25D to CYP24A1 located in the
inner mitochondrial membrane. The heme group (red dotted
sphere) reduces oxygen to hydroxylate 1,25D.22
can explain how vitamin D regulates both nongenomic and genomic response via specific
ligand-binding pockets.
VDR fits into the nuclear receptor superfamily, which isare a class of
transcriptional regulators in animals. Nuclear receptors are ligand-activated; in the case of
VDR, vitamin D would be the ligand that activates transcription. Tissues that contain
VDR (over 37) define specific locations where vitamin D can initiate biological
responses. Some of these responses include
the classic calcium homeostasis system,
Figure 4: Shapes of the optimal ligands for VDRmediated responses and for RR, as well as for vitamin D
binding protein (DBP). There is a characteristic ligand
shape for each type of response.
along with five other systems, including the brain, whichh we will be focused on later.
Thee ligand-receptor complex is what produces the biological reactions.
After the ligand binds to the VDR genomic pocket (GP), there is a conformational change
to allow it to serve as a platform for coactivator binding. The coactivator allosterically
stabilizes the VDR-RXR heterodimer, then allowing it to be phosphorylated by serine
protein kinases. This new complex can positively and negatively regulate gene
transcription by recognizing vitamin D response elements (VDREs) in DNA. The VDRRXR then recruits additional comodulators to help initiate transcription. There are many
hypotheses concerning how exactly thisof how exactly happensthis happens; Dr.
Hausslers team proposes that there is a simultaneous binding of multiple factors in a
from finding the genes that are directly regulated by this VDR complex. So far, at least
eleven genes that encode bone and mineral homeostasis (the traditional target of VDR)
have been found, including gene products that facilitate intestinal calcium intake. Another
network that has been found to be regulated by VDR is theare encoding factors that
impact cell life/cancer, the immune system, and metabolism. These come from inducing
and repressing various genes involved in diseases such as type I diabetes, multiple
sclerosis, and arthritis. It has even been found to blunt various genes involved in
inflammatory responses, thus reducing the risk of heart disease and Alzheimers. It is
clear that there are many areas regulated by VDR, and there will certainly be more to
come.
D showed the same binding affinities to VDR in the caveolae as observed with nuclear
VDR, and that vitamin D localized in vivo in the plasma membrane caveolae9.14
Finally, the VDR AP site was proposed to resolve the VDR paradox (see Figure
7).23 Traditionally, only a
single ligand binding
Figure 6: Different mechanisms by which vitamin D and VDR can induce chemical
responses in the body. On the left, the caveolae-related pathway leads to activation of the
secondary messenger system to elicit short term responses. On the right, 1,25D can
interact with VDR localized in the cell nucleus to produce genomic responses through
gene transcription.
Figure 7: The proposed VDS-VDR conformational ensemble model. The left panel
shows the conformational flexibility of VDS, the middle panel shows the different
Figure 7: The proposed VDS-VDR conformational ensemble model. The left panel shows the
binding sites on VDR, with the yellow oval showing overlap between the two regions.
conformational flexibility of VDS, the middle panel shows the different binding sites on VDR,
The right panel shows specific conformational dynamics of VDR AF2 domain; the
with the yellow oval showing overlap between the two regions. The right panel shows specific
Boltzmann distribution is altered depending on the nature of the ligand, changing the
conformational dynamics of VDR AF2 domain; the Boltzmann distribution is altered depending
energy landscape of VDR ensemble members to bias a specific downstream event.
on the nature of the ligand, changing the energy landscape of VDR ensemble members to bias a
specific downstream event.
binding site. Computational work showed that there is an alternative binding site
available. This conformational model was proposed by Dr. Haussler and his team,
whereby the VDR could accommodate differently shaped ligands to initiate both genomic
and rapid responses. The steroid hormone would essentially test the waters and form a
receptor-hormone complex with the receptor species that provided the highest affinity
and most stability. Figure 86 shows the main differences between the genomic and rapid
response pathways.150. It seems as though the two categories are vastly different, but a
small portion of VDRs at the membrane is now believed to regulate the expression of
genes, thus regulating the activity of many kinases, phosphatases, and ion channels.
However, more research needs to be done to further elucidate the mechanisms behind this
process.
Implications of
Vitamin D/VDR
Figure 86: Different mechanisms by which vitamin D and VDR can induce chemical
responses in the body. On the left, the caveolae-related pathway leads to activation of the
secondary messenger system to elicit short term responses. On the right, 1,25(OH) 2D
1,25D can interact with VDR localized in the cell nucleus to produce genomic responses
through gene transcription.
and Sleep:
Since VDR has recently been found in the brain, some interesting new hypotheses
have emerged concerning the function of vitamin D in the brain.181. One of these concerns
the role of vitamin D in sleep. Normally, sleep is highly organized. Humans typically go
to sleep at the same time every night, going through specific phases, such as REM, slowwave, etc. Waking up from sleep is involuntary and also occurs in a fairly stable manner.
This seems to imply that sleep is not caused by a buildup of sleep-inducing hormones and
substances; rather, it is a result of a circadian rhythm type function where sleep is highly
coordinated by the time of day. This implies that one of the reasons for sleep problems
stems from brain chemistry.
Normally, many different hormones are secreted before sleep, leading to
drowsiness a warning, so to speak. Antidiuretic hormone is produced to limit urine
production, melatonin levels increase, and so on. The brain also induces paralysis as deep
sleep arrives, activating specific neurotransmitters to turn off the signals responsible for
wakefulness. These two categories, timing and paralysis, are essential to sleep. Saper and
colleagues suggest an on-off switch mechanism, which is responsible for sleep; one part
of the brainstem is active while the other is suppressed.162 Specifically, the hypothalamus
is thought to be involved because the stimulation of the posterior hypothalamus induces
arousal, while stimulation of the anterior hypothalamus and adjacent basal forebrain
region causes sleep.1317. Now, how does this tie in with vitamin D? Vitamin D targeted
neurons have been discovered in specific brain and spinal cord locations in multiple
animals. This suggests a possible role of vitamin D in regulating sleep. A 2-year
uncontrolled trial of vitamin D supplementation in 1500 patients with neurological
complaints and sleep problems saw improvements in both these functions.14 18 Further
research needs to be done in this area, as sleep is also influenced by sociological and
psychological factors. For example, pain has been shown to influence the quality of sleep,
but pain has also been linked to vitamin D.195 Therefore, vitamin D may ameliorate the
quality of sleep through a multitude of factors not only through chemical pathways in
the brain, but also through alleviating pain. There is a delicate balance between these
factors; vitamin D could directly impact sleep, which could then improve feelings of
pain. Vitamin D could also improve a multitude of variables, including mood, quality of
life, etc. which could also improve pain (a subjective feeling that could be influenced by
psychology). Further research needs to be done to elucidate the function of vitamin D in
these processes.
Vitamin D has clearly grown in importance over the last half century. The
involvement of vitamin D in vital bodily processes, from bone health to even regulating
gene expression, reveals how potent this single hormone is to human health. New models
to elucidate the nature of vitamin D and VDR binding will further this cause and may
even reveal new routes for drug development and helping curethe curing of diseases, one
of the most fundamental concerns for the human race.
References
T.H.J. Burnes, and J.J. McGrath, Frontiers of Neuroendocrinology 2013, 34, 4764.
12.
Haussler, Mark R., and Kerr Whitfield. "Molecular Mechanisms of
Vitamin D Action." Calcified Tissue International 92.2 (2013): 7798. Springer Link. Web. 15 May 2013.
<http://link.springer.com/article/10.1007%2Fs00223-012-9619-0>.
13.
Vitamin D receptor: molecular signaling and actions of nutritional
ligands in disease prevention Mark R Haussler, Carol A Haussler, Leonid Bartik,
et al., Nutrition Reviews 2008, 66, S98-S112.
14.
Johanna A. Huhtakangas, Christopher J. Olivera, June E. Bishop, Laura P.
Zanello, and Anthony W. Norman. The Vitamin D Receptor Is Present in
Caveolae-Enriched Plasma Membranes and Binds 1 ,25(OH)2-Vitamin D3 in
Vivo and in Vitro Molecular Endocrinology 2004 18: 2660-2671;
doi:10.1210/me.2004-0116
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The Vitamin D SterolVitamin D Receptor Ensemble Model Offers
Unique Insights into Both Genomic and Rapid-Response Signaling Mathew
T. Mizwicki and Anthony W. Norman Sci. Signal 2009, 2 (75), re4. [DOI:
10.1126/scisignal.275re4]
16.
Saper, Cliff B., and Patrick M. Fuller. Sleep State
Switching. Neuron 2011 6.68, 1023-42. Print.
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1997. Web. 15 May 2013.
<http://www.sleepsources.org/uploads/sleepsyllabus/e.html>.
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Gominak, S.C., and W.E. Stumpf. The world epidemic of sleep disorders
is linked to vitamin D deficiency. Medical Hypotheses 2012 79.2: 132-35. Print.
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Roehers, Timothy, and Thomas Roth. Sleep and Pain: Interaction of Two
Vital Functions. Seminars in Neurology 2005, 2.1: 106-116. Print.
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ECMDB: The E. coli Metabolome Database. Guo AC, Jewison T, Wilson
M, Liu Y, Knox C, Djoumbou Y, Lo P, Mandal R, Krishnamurthy R, Wishart DS.
Nucleic Acids Res. 2012 Oct 29. [Epub ahead of print] PMID: 23109553.
21.
Crystal structures of the vitamin D-binding protein and its complex with
actin: Structural basis of the actin-scavenger system Ludovic R. Otterbein,
Christophe Cosio, Philip Graceffa, et al. PNAS 2002, 99, 8003-8008.
22.
Crystal Structure of CYP24A1, a Mitochondrial Cytochrome P450
Involved in Vitamin D Metabolism Andrew J. Annalora, David B. Goodin, WenXu Hong, et al. Journal of Molecular Biology 2010, 396, 441-451.
23.
D
1 http://www.cancer.gov/cancertopics/factsheet/prevention/vitamin-D
2 http://ajcn.nutrition.org/content/95/6/1357.long
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JOSHUA A. DESOTELLE, MELISSA J. WILKING , AND AHMAD NIHAL,
PHOTOCHEMISTRY AND PHOTOBIOLOGY 2012, 88, 1037-1047.
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NEUROPSYCHIATRIC DISEASE D.W. EYLES, T.H.J. BURNES , AND J.J.
MCGRATH, FRONTIERS OF NEUROENDOCRINOLOGY 2013, 34, 47-64.