Beruflich Dokumente
Kultur Dokumente
Doering 6/2004
5. Run the plate. Open the tank and gently put your plate in, samples down. Avoid splashing into the
solvent, and prop the bottom edge against the glass spine and the top edge against the filter paper liner. Keep
an eye on the solvent front until you get to know the system, and remove the plate when it reaches your
solvent front line.
Some protocols call for several developments, to improve separation near the origin. In this case remove
the plate, place in a hood or under a hair dryer until it is completely dry, and then put it back in the tank.
(Keep the tank closed in between.)
6. Analyze the data. As appropriate scan the plate and/or spray with EnHance and expose to film for hot
samples. For non-radioactive samples spray with appropriate stain and visualize as directed in your protocol.
For hot samples you may want to draw small (~1 mm diam) circles at two places on the plate, then spot a
l of leftover hot liquid in that point after running but before spraying. These hot spots will show on
the film and allow you to align the film with the TLC plate to know where samples and standards were.