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The Study Proposal

A. Preliminary Page
1. Cover or Title Page - consists of proposed study title, submission
statement, degree statement, authors or authors name(s), month and
year of expected completion.
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VANCOMYCIN RESISTANT Staphylococcus aureus AMONG


MEDICAL TECHNOLOGY INTERNS AT A TERTIARY
HOSPITAL IN MANILA, PHILIPPINES
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]
10 - 12 single spaces
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Undergraduate Thesis
Submitted to the Faculty of the
Department of Medical Technology
College of Nursing
Cavite State University
Indang, Cavite
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10 single spaces
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In partial fulfillment
of the requirements for the degree
Bachelor of Science in Medical Technology
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10 - 12 single spaces
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MARY LYNETH C. APURA arranged
REY A. JAVIER JR.
alphabetical
PETER PAUL M. RAMOS
ly
1 single space only
April 2014
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2. Approval sheet - contains the university logo with the letterhead, name of
college, department, authors or authors name(s), proposed study title,
names of signatories and dates.
----------------------------------------------------------------------------------------------------------------------------------------Republic of the Philippines
CAVITE STATE UNIVERSITY
Don Severino de las Alas Campus
Indang, Cavite
(046) 4150-013 loc 232
www.cvsu.edu.ph
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2
COLLEGE OF NURSING
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2
Department of Medical Technology
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3 - 4 single spaces
]
Authors:
APURA, MARY LYNETH C.
JAVIER JR., REY A.
RAMOS, PETER PAUL M.
1
2
Title:
VANCOMYCIN RESISTANT Staphylococcus aureus AMONG MEDICAL
TECHNOLOGY INTERNS AT A TERTIARY HOSPITAL IN MANILA,
PHILIPPINES
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2
3
APPROVED:
4 - 7 spaces below the
1
proposed title
2
3
RONALYN S. SANCHEZ, RMT
_____
ADELAIDA E. SANGALANG, PhD _____
Adviser
Date
Technical Critic
Date
1
2
3
FLORDELIZA A. DE GUZMAN, RMT, MAT
_____ NENITA B. PANALIGAN, RN, MAN _____
Department Chairman
Date
College Research Coordinator
Date
1
2
3
4
LENILA A. DE VERA, RN, MPH, MAN _______
Dean
Date
Note: Original copy to be filed in the University Library and one copy to be distributed to the following: Adviser,
Technical Critic. University Research Center, College Research Coordinator, College Library and Thesis students.

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B. Body of the Proposal


1. Introduction
- give an overview of the study and catch the attention of the reader
- contains the study title, authors or authorsname(s) and manuscript
ID
a. Background of the study or introductory paragraph
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ANTIMICROBIAL PROPERTY OF Bauhinia monandra LEAF EXTRACTS


AGAINST SELECTED PATHOGENS

Junee Elleigh C. Oway


Yrhiz F. Oway
Lindsay Marie V. Reyes

A undergraduate thesis proposal submitted to the faculty of the Department of Medical


Technology, College of Nursing, Cavite State University, Indang, Cavite in partial fulfilment of
the requirements for the degree of Bachelor of Science in Medical Technology with Contribution
No. ________________, Prepared under the supervision of Dr. Adelaida E. Sangalang.

INTRODUCTION

Plants were being used up in various studies to uncover any therapeutic property to fight
off diseases especially to eradicate the microorganisms which cause such diseases. These studies
were made on plants which are locally available. Bauhinia monandra is a native of south eastern
Asia and is widely planted in the Philippines as an ornamental flowering tree. It grows 3 to 15.2
m in height and 0.5 m in diameter. The leaves are deeply lobed and are shaped like butterfly
wings. The smooth gray bark of the tree becomes scaly and reddish brown as it grows older
(Connor, 2002).

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Not much information is known about Bauhinia monandra but discovery of this plants
uses is still in progress. It is used traditionally as a treatment for diabetes in Brazil. This
hypoglycemic activity was evaluated in a study conducted by Alade et al. (2011).
---------------------------------------------------------------------------------------------------------------------------------------b. Statement of the problem
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Statement of the Problem


This study determined the hypoglycemic effects of Angelica keiskei koidzumi (ashitaba)
and Annona squamosa Linn. (atis) on alloxan-induced diabetic male mice.
Specifically, it answered the following questions:
1. What are the blood glucose concentrations of the mice before and after weekly

administration of ashitaba tea and atis tea?


2. Is there a significant difference between the effects of ashitaba tea and
---------------------------------------------------------------------------------------------------------------------------------------c. Objectives of the study
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Objectives of the Study


Generally, this study aims to determine the antimicrobial property of Bauhinia monandra
against selected microorganisms.
Specifically, this study aims to:
1. determine the constituents of Bauhinia monandra through phytochemical analysis;
2. determine which plant extract will be more potent as an antimicrobial agent; and
3. determine which pathogen will be most susceptible to the plant extract.

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---------------------------------------------------------------------------------------------------------------------------------------d. Significance of the study


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Significance of the Study


This study can be used to establish the antimicrobial property of Bauhinia monandra
against selected pathogens which will be utilized as the test subjects. This plant is a convenient
source of antimicrobial agent as it can be seen locally. It can also be used as a basis of new drugs
and in providing natural remedy to diseases caused by pathogens developing resistance to
multiple drugs.
People of the community will benefit from this study because it is just planted along the
highways as ornamental plant. If proven effective, they can continue using the leaves of this
plant as a treatment for certain
---------------------------------------------------------------------------------------------------------------------------------------e. Time and place of the study
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Time and Place of the Study


The leaves of Bauhinia monandra will be collected at Tagaytay City, Cavite beside
Tagaytay Sports Complex. Samples will be sent to University of the Philippines Los Baos
Botanical Herbarium for authentication in Los Baos, Laguna. Then, the samples will be
submitted to the Department of Science and Technology in Taguig, Metro Manila for
phytochemical analysis. Rotary evaporation method will be done at De La Salle University
Dasmarias Interdisciplinary Science and Research Unit in Dasmarias, Cavite to concentrate
the crude extract. The selected pathogens used in the study will be procured from the National

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Institute of Molecular Biology and Biotechnology, University of the Philippines Los Baos. The
experimentation will be conducted at the laboratory of the Department of Medical Technology,
College of Nursing, Cavite State University, Indang, Cavite from February to October 2015.
---------------------------------------------------------------------------------------------------------------------------------------f. Scope and limitation of the study
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Scope and Limitations of the Study


This study will only use hexane and ethanol leaf extracts of Bauhinia monandra against
selected pathogens to serve as test subjects which includes Escherichia coli, Bacillus subtilis,
Candida tropicalis and Fusarium oxysporum. This will be conducted in view of obtaining
pertinent information on the antimicrobial property of Bauhinia monandra leaf extracts.
---------------------------------------------------------------------------------------------------------------------------------------g. Definition of Terms
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Definition of Terms
To explain the following terms simply, the following are defined:
Hypoglycemic effect refers to the components present in the plant or drug that can lower
blood sugar levels.
Angelica keiskei koidzumi or simply ashitaba is a herbal plant native in the island of
Hachijo in Japan. Its yellow sap contains chalcones (4-hydroxyderricin and xanthoangelol) with
hypoglycemic effects.
Annona squamosa Linn. or simply atis is a tree native to the West Indies and South
America. Its leaves have quercetin-3-O-glucoside that possesses hypoglycemic property.

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FDA ICR (Food and Drug Administration Institute of Cancer Research) strain Mus
musculus is a ten to twelve week old albino mice purchased at the Food and Drugs
Administration in Alabang, Metro Manila.
---------------------------------------------------------------------------------------------------------------------------------------h. Theoretical and/or conceptual framework of the study
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---------------------------------------------------------------------------------------------------------------------------------------2. Review of Related Literature


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REVIEW OF RELATED LITERATURE

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Bauhinia species
As cited by Sousa Menezes et al. (2007), Bauhinia species encompass 300 plants cultivated
worldwide in tropical areas. In Brazil and in other countries, aqueous extracts of leaves, stems,
and roots of Bauhinia species specifically B. cheilantha, B. rufescens, B. ungulata, B. forficata,
B. splendens, and B. glabra are widely used for the treatment of several diseases including
infections, pain processes and diabetes. In Africa, Asia, South America and Central America,
several Bauhinia species are utilized as folk medicines. A wide range of compounds have been
isolated and identified, mainly lactones, flavonoids, terpenoids, glicolipids, glucosyl steroids,
steroids, tannins and quinines.
Studies on the Other Species of the Genus Bauhinia
In the study conducted by Sardessai et al. (2013), the n-hexane extract of the stem bark of
Bauhinia purpurea was screened for phytochemical and antimicrobial property. The presence of
fatty acids, triterpenoids, steroids, alkaloids and phytol esters was revealed in the preliminary
phytochemical studies. They used Well Diffusion and Tube Dilution Method in carrying out the
antimicrobial screening of n-hexane extract with 6 bacterial strains (Bacillus subtilis,
Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium,
Klebsiella species) and 2 fungal strains (Aspergillus niger and Claviceps purpurea) as test
subjects. The study found the n-hexane extract to be significantly effective towards bacterial as
well as fungal strains. They attributed the antimicrobial activity of the plant to its bioactive
constituents.

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---------------------------------------------------------------------------------------------------------------------------------------3. Methodology
Experimental research
a. Materials
b. Methods
i. Experimental units
ii. Experimental design
iii. Treatments
iv. Lay-out of the experiment
v. Data to be gathered
vi. Statistical treatment of data
---------------------------------------------------------------------------------------------------------------------------------------Please check the different style of presenting the methodology that will fit in your
study.
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Social research
b. Research design
c. Hypothesis
d. Sources of data
e. Participants of the study
f. Sampling technique
g. Data to be gathered
h. Statistical treatment of data

4. References
- Use APA 6th edition or Zotero app in citing your references.
- All references should be arranged alphabetically.
C. Appendices
1.
2.
3.
4.
5.
6.

Research instruments
Data sheets
Letter requests
Memorandum of agreement
Timetable of activities
Budgetary requirements of the study

OTHER CONCERNS
1. All research paper should be computerized and printed in a letter size white
bond paper.

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2. The font style should be Times New Roman, size 12. Font size less than 12
can be used in tables with numerous entries.
3. The left margin of each page should be 1.5 in, while the top, bottom and right
should be 1 inch each.
4. Each paragraph should be indented 5 single spaces (or one tab) from the left
margin.
5. Three single spaces should be used between the chapter headings and the
text that follows. The rest should be double space.
6. Each subtopic title should be written 2 single spaces below the last part of
the preceding topic.
7. The references should be written in hanging indent format, single-spaced and
flushed to the left margin.
8. The page numbers should be places at the top right of the page. The first
page of every chapter should have imaginary page numbers,
9. For tables, follow the format seen below. Use 1 point line for all horizontal
lines and maintain 1.5 spaces between entries. In case that the tables are
split in separate pages, the next half should be titles Table #. Continued..
10.For the figures, follow the format seen below. In case that the figure is too
large, it can be reduced.
11.All non-English terms and scientific names should be italicized.
12.SI units should be used. If the units are not preceded by Arabic numbers, it
should be spelled out.
13.Always spell out numbers 1 to 9, while 10 and above should be written in
Arabic numerals. Cardinals are also spelled out.
14.If the sentence begins with a figure, it should be spelled out.
15.Fractions should be spelled out except when preceded by a whole number.
16.All acronyms and abbreviated terms in parentheses should occur only after
their first reference in the text.
17.All proper nouns are capitalized.
18.Use present tense for presenting theories, philosophies, generalizations and
conclusions. Use past tense in discussing the results of the study. Use future
tense when writing your outline for proposal defense.

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