Beruflich Dokumente
Kultur Dokumente
project
report
Certificate
This is to certify that Arpan Jain, student of class XII- C, Lovely Public
Sr. Sec. School has completed the project titled To analyse various
juices for detection of anions, cations, starch, fats, proteins during the
academic year 2015-2016 for the chemistry practical evaluation and
submitted satisfactory report as compiled in the following pages under
my supervision.
Acknowledgement
I would like to express my sincere gratitude to my chemistry teacher
Mrs. Mamta Rampal for the vital support, guidance and encouragement
without which this project would not have come forth. I would also like
to thanks my project partner Pranav Aswal for his co-operation.
Aim:
To analyse various juices (tomato, spinach, onion) for the purpose of
detecting anions, cations, sugars, starch and protein .
Materials used:
Test tubes, test tube stand, test tube holder, bunsen burner, spatula,
beaker.
JUICES:
Tomato juice
Spinach leaves juice
Onion juice
Chemicals:
Fehlings A and Fehlings B, distilled water, several other lab chemicals
and reagents.
INTRODUCTION
A balanced diet is essential for the purpose growth and maintenance of
the body. A balanced diet contains about 60% carbohydrate, 20% fat,
15% proteins and small amount of vitamins and minerals. Fruits and
vegetables also contain acids and minerals. The amount and kind of acid
present in these juices differ. Some contain complex organic molecule
and some flavoring agents like esters and aromatic compounds.
OBSERVATIONS:
Observation
Inference
Brisk effervescence
May be CO32-
CO32- confirmed
May be Cl-
White ppt.
Cl- confirmed
Juice + Na2CO3 +
conc. HCl + BaCl2
No ppt.
Sulphate
absent
Identification of Cations
(TOMATO JUICE)
Experiment
Observation
Inference
Juice + NaOH
No reaction
NH4+ absent
No reaction
Pb2+ absent
No reaction
Juice + NH4Cl
+ NH4OH
Al3+ confirmed
Juice + NH4Cl +
NH4OH filter
solution and pass
through H2S
No reaction
No reaction
Tests:
May be Al3+
Ba2+,Sr2+ , Ca2+
absent
(TOMATO JUICE)
Experiment
Juice + Fehling A and
Fehling B and heat
Observations
Red ppt.
Yellow colour
Insoluble
Inference
Sugar confirmed
Starch absent
Protein confirmed
Fat confirmed
ANALYSIS OF ONION
Identification of Anions
(Onion juice)
Experiment
Observation
No reaction
Inference
CO32-, F- , SO32- ,
NO2- absent
_
Juice + conc.
H2SO4
Brown gas
May be NO3 or Br _
NO3 confirmed
SO42- absent
PO43- present
Identification of Cations
(Onion juice)
Experiment
Observation
Juice + NaOH
Inference
NH4+ absent
No reaction
Pb2+ absent
No reaction
No reaction
Juice + NH4Cl +
Nh4oh
May be Al3+
Al3+ confirmed
No reaction
Ni2+,Po2+,Mn2+, Zn2+
absent
No reaction
Observation
Inference
Red colour
Sugar confirmed
No colour
Starch absent
Yellow colour
Protein present
Soluble
Fat absent
Fehlings test
Analysis of Spinach
IDENTIFICATION OF ANIONS
(Spinach leaves juice)
Experiment
Observation
Inference
No reaction
Juice + FeCl3
NO3 - confirmed
May be SO4 2-
SO42- confirmed
IDENTIFICATION OF CATIONS
(Spinach leaves juice)
Experiment
Observation
Inference
No reaction
NH4 + absent
No reaction
Juice + NH4Cl +
NH4OH
No reaction
Ni2+,Co2+
Mn2+, Zn2+ absent
No reaction
Experiment
Observation
Inference
Sugar absent
Starch absent
Yellow colour
Protein confirmed
Soluble
Fat absent
RESULT OF ANALYSIS
Topic
TOMATO
ONION
Spinach
Anions present
Cations present
23
34
CO , Cl
PO
Al3+
CO
2-4
, NO3
Al3+
NO3- , SO42-
Fe2+
Sugar
Starch
Protein
FAT
CONCLUSION
1. Proteins are present in all.
2. Sugar is present in onion and tomato.
3. Fat is present only in tomato.
PRECAUTIONS
1. Juice should be properly sieved.
2. Juice should be fresh.
3. Test tube should be clear and should be held in holders.
4. Cations should be tested systematically from the zero group
onwards.