ISO9001:2000 Certified

Material Safety Data Sheet

Methylene Blue Reagent
Section 1 - Chemical Product and Company Identification
MSDS Name:
Methylene Blue Reagent
Catalog Numbers:
LC16970
Synonyms:
Methylene blue reagent for surfactants
Company Identification:
LabChem, Inc.
200 William Pitt Way
Pittsburgh, PA 15238
Company Phone Number:
(412) 826-5230
Emergency Phone Number:
(800) 424-9300
CHEMTREC Phone Number:
(800) 424-9300

Section 2 - Composition, Information on Ingredients

CAS#
61-73-4

Chemical Name:
Methylene blue

Percent
0.003

7558-80-7

Sodium phosphate, monobasic

7664-93-9

Sulfuric acid

7732-18-5

Water

balance

Section 3 - Hazards Identification

Emergency Overview

Appearance: blue solution

Warning! Causes respiratory tract irritation. Causes eye and skin irritation. May be harmful if
swallowed. Reproductively active.
Target Organs: Blood, eyes.

Potential Health Effects

Eye:
Causes eye irritation. May cause chemical conjunctivitis.
Skin:
Causes mild skin irritation.
Ingestion:
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause hemolytic anemia.
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Material Safety Data Sheet

Methylene Blue Reagent
Inhalation:
May cause respiratory tract irritation. May cause methemoglobinemia, cyanosis (bluish
discoloration of skin due to deficient oxygenation of the blood), convulsions, tachycardia, dyspnea
(labored breathing), and death. Can produce delayed pulmonary edema.
Chronic:
Effects may be delayed. Laboratory experiments with methylene blue have resulted in mutagenic
effects. Repeated contact may cause corneal damage. May cause adverse reproductive effects.

Section 4 - First Aid Measures

Eyes:
Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper
and lower eyelids. Get medical aid.
Skin:
Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing
contaminated clothing and shoes. Wash clothing before reuse.
Ingestion:
Never give anything by mouth to an unconscious person. Get medical aid. Do NOT induce
vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water.
Inhalation:
Remove from exposure and move to fresh air immediately. If not breathing, give artificial
respiration. If breathing is difficult, give oxygen. Get medical aid. Do not use mouth-to-mouth
resuscitation.
Notes to Physician:
Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures

General Information:
Material will not burn. During a fire, irritating and highly toxic gases may be generated by thermal
decomposition or combustion. Wear appropriate protective clothing to prevent contact with skin and
eyes. Wear a self-contained breathing apparatus (SCBA) to prevent contact with thermal
decomposition products. Use extinguishing media appropriate to the surrounding fire.
Extinguishing Media:
Substance is noncombustible; use agent most appropriate to extinguish surrounding fire.
Autoignition Temperature:
No information found.
Flash Point:
No information found.
NFPA Rating:
Not available
Explosion Limits:
Lower: n/a
Upper: n/a

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Material Safety Data Sheet

Methylene Blue Reagent
Section 6 - Accidental Release Measures
General Information:
Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks:
Vacuum or sweep up material and place into a suitable disposal container. Clean up spills
immediately, observing precautions in the Protective Equipment section. Avoid generating dusty
conditions. Provide ventilation.

Section 7 - Handling and Storage

Handling:
Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with
eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Use with
adequate ventilation. Wash clothing before reuse.
Storage:
Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible
substances.

Section 8 - Exposure Controls, Personal Protection

Engineering Controls:
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety
shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the
permissible exposure limits.
Exposure Limits:

Chemical Name:

Methylene blue
Sodium phosphate
monobasic
Sulfuric acid
Water

ACGIH

NIOSH

OSHA

none listed
none listed

none listed
none listed

none listed
none listed

0.2 mg/m3 TWA

(thoracic fraction)
none listed

1 mg/m3 TWA
15 mg/m3 IDLH
none listed

1 mg/m3 TWA
none listed

OSHA Vacated PELs:

Methylene blue: No OSHA Vacated PELs are listed.
Sodium phosphate monobasic, anhydrous: No OSHA Vacated PELs are listed for this chemical.
Water: No OSHA Vacated PELs are listed for this chemical.
Sulfuric acid: 1 mg/m3 TWA

Personal Protective Equipment

Eyes:
Do not wear contact lenses when working with chemicals. An eye wash fountain should be
available in the immediate work area. Wear appropriate protective eyeglasses or chemical safety
goggles as described in 29 CFR 1910.133.
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Material Safety Data Sheet

Methylene Blue Reagent
Skin:
Wear appropriate protective gloves to prevent skin exposure.
Clothing:
Wear appropriate protective clothing to prevent skin exposure.
Respirators:
Follow the OSHA respirator regulations found in 29 CFR 1910.134. Use a NIOSH/MSHA
approved respirator if exposure limits are exceeded or if irritation or other symptoms are
experienced.

Section 9 - Physical and Chemical Properties

Physical State:
Color:
Odor:
pH:
Vapor Pressure:
Vapor Density:
Evaporation Rate:
Viscosity:
Boiling Point:
Freezing/Melting Point:
Decomposition Temperature:
Solubility in water:
Specific Gravity/Density:
Molecular Formula:
Molecular Weight:

Liquid
Blue
Odorless
Acidic
Not available
Not available
Not available
Not available
Not available
Not available
Not available
Soluble
1.1 (Water = 1)
Not applicable
Not applicable

Section 10 - Stability and Reactivity

Chemical Stability:
Stable under normal storage and handling conditions.
Conditions to Avoid:
Excess heat and light, incompatible materials.
Incompatibilities with Other Materials:
Oxidizing agents, reducing agents, bases, dichromates, alkali iodides, caustic alkalis.
Hazardous Decomposition Products:
Chlorine, nitrogen oxides, carbon monoxide, oxides of sulfur, oxides of phosphorus, irritating and
toxic fumes and gases, carbon dioxide.
Hazardous Polymerization:
Has not been reported.

Section 11 - Toxicological Information

RTECS:
CAS# 61-73-4: SO5600000
CAS# 7558-80-7: WA1900000
CAS# 7664-93-9: WS5600000
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Material Safety Data Sheet

Methylene Blue Reagent
CAS# 7732-18-5: ZC0110000
LD50/LC50:
CAS# 61-73-4:
Oral, mouse: LD50 = 3500 mg/kg;
Oral, rat: LD50 = 1180 mg/kg
CAS# 7558-80-7:
Draize test, rabbit, eye: 150 mg Mild;
Oral, rat: LD50 = 8290 mg/kg
CAS# 7664-93-9:
Draize test, rabbit, eye: 250 ug Severe;
Inhalation, mouse: LC50 = 320 mg/m3/2H;
Inhalation, mouse: LC50 = 320 mg/m3;
Inhalation, rat: LC50 = 510 mg/m3/2H;
Inhalation, rat: LC50 = 510 mg/m3;
Oral, rat: LD50 = 2140 mg/kg
CAS# 7732-18-5:
Oral, rat: LD50 = >90 mL/kg
Carcinogenicity:
CAS# 61-73-4: Not listed by ACGIH, IARC, NTP, or CA Proposition 65.
CAS# 7558-80-7: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 7664-93-9:
ACGIH: A2 - Suspected Human Carcinogen (contained in strong inorganic acid mists)
California: carcinogen, initial date 3/14/03 (listed as Strong inorganic acid mists containing
sulfuric acid).
NTP: Known carcinogen (listed as Strong inorganic acid mists containing sulfuric acid).
IARC: Group 1 carcinogen
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
Epidemiology:
No information available.
Teratogenicity:
No information available.
Reproductive:
CAS# 61-73-4: Oral, rat: TDLo = 2500 mg/kg (female 1-22 day(s) after conception) Fertility - postimplantation mortality (e.g. dead and/or resorbed implants per total number of implants).
Mutagenicity:
CAS# 61-73-4: DNA Adduct: Mouse, Ascites tumor = 70 umol/L.; DNA Adduct: Mammal species unspecified Lymphocyte = 1830 nmol/L.
Neurotoxicity:
No information available.

Section 12 - Ecological Information

No information available.

Section 13 - Disposal Considerations

Dispose of in accordance with Federal, State, and local regulations.
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Material Safety Data Sheet

Methylene Blue Reagent
Section 14 - Transport Information
US DOT

Shipping Name: Not regulated.

Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information

US Federal

TSCA:
CAS# 61-73-4 is listed on the TSCA inventory.
CAS# 7558-80-7 is listed on the TSCA inventory.
CAS# 7664-93-9 is listed on the TSCA inventory.
CAS# 7732-18-5 is listed on the TSCA inventory.
SARA Reportable Quantities (RQ):
CAS# 7664-93-9: 1000 lb. final RQ; 454 kg final RQ
CERCLA/SARA Section 313:
Sulfuric acid is not at a high enough concentration to be reportable under Section 313.
OSHA - Highly Hazardous:
Not considered highly hazardous by OSHA.

US State

State Right to Know:

CAS# 61-73-4 is not present on state lists from California, Pennsylvania, Minnesota, Massachusetts,
Florida, or New Jersey.
CAS# 7558-80-7 is not present on state lists from California, Pennsylvania, Minnesota,
Massachusetts, Florida, or New Jersey.
CAS# 7664-93-9 can be found on the following state right to know lists: California, New Jersey,
Pennsylvania, Minnesota, Massachusetts.
CAS# 7732-18-5 is not present on state lists from California, Pennsylvania, Minnesota,
Massachusetts, Florida, or New Jersey.
California Regulations:
WARNING: This product contains Sulfuric acid, listed as `Strong inorganic acid mists containing
sulfuric acid', a chemical known to the state of California to cause cancer.

European/International Regulations

Canadian DSL/NDSL:
CAS# 61-73-4 is listed on Canada's DSL List.
CAS# 7558-80-7 is listed on Canada's DSL List.
CAS# 7732-18-5 is listed on Canada's DSL List.
CAS# 7664-93-9 is listed on Canada's DSL List.

Canada Ingredient Disclosure List:

CAS# 61-73-4 is listed on the Canadian Ingredient Disclosure List.
CAS# 7558-80-7 is not listed on the Canadian Ingredient Disclosure List.
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Material Safety Data Sheet

Methylene Blue Reagent
CAS# 7664-93-9 is listed on the Canadian Ingredient Disclosure List.
CAS# 7732-18-5 is not listed on the Canadian Ingredient Disclosure List.

Section 16 - Other Information

MSDS Creation Date: August 7, 2006
Revision Date: None
Information in this MSDS is from available published sources and is believed to be accurate. No warranty, express or implied,
is made and LabChem Inc. assumes no liability resulting from the use of this MSDS. The user must determine suitability of this
information for his application.

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