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Kultur Dokumente
ABSTRACT
Mango (Mangifera indica L.) is one of the most important tropical fruit crop. Most studies on exploitation of mango have been dealing with mango leaves, juice and bark, however little attention given to mango peel and latex. Biologically active compounds from
vegetal origins are a possible source of natural antifungic effect. Especially, unripe mango contains constitutive defence metabolites
against pathogens and induces quiescent. An n-hexane extraction was used to obtain bioactive metabolites from Mangifera indica L.
cv. Neelum unripe fruit peel and latex. Antifungal effectiveness was determined by challenging the extracts from the best extraction
treatment against two post-harvest fungal pathogens viz., Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The peel
and latex extract exhibited the broadest action spectrum against post-harvest pathogens. The n-hexane extract from Mangifera indica L. cv. Neelum unripe fruit peel and latex were subjected to TLC and GC/MS analysis for compound profiling. These compounds
were resolute as potential source of secondary metabolites with antifungal properties against invading fungal pathogens.
INTRODUCTION
Mango (Mangifera indica L.) is considered one
of the most popular fruits among millions of people in the
tropical area and increasingly in the developed countries.
Because of its delicious taste and high caloric value, it is
ranked as one of the good fruits in the international market.
This fruit has become an essential fruit crop in Asia, Southern
and Central America as well as in many parts of Africa.
Because of diverse production conditions and the vast area
grown, mango suffers from a number of diseases, some of
them taking heavy toll on the crop and representing limiting
factors. These diseases include anthracnose and stem end rot,
all largely caused by different fungi, mainly Colletotrichum
gloeosporioides and Lasiodiplodia theobromae. Synthetic
fungicides are essential to effectively controlling fungus
attacks on fruit. However, some of these fungicides are toxic
in the environment and to mammals that come into contact
with them, and their efficiency can be reduced as fungi
develop resistance due to improper application (Spalding,
1982). This demand has generated increased interest in the
potential of biological control of pathogens using vegetal
extracts containing secondary metabolites. Use of vegetal
extracts with antifungal properties has been a common
practice for thousands of years, for example, powders or
extracts of acacia, garlic, eucalyptus and mint all function
as fungicides capable of controlling different diseases.This
recent focus on natural management of phytopathogenic
fungi has been reflected in extensive research on biological
fungicides. Extracts from different mango tissues have been
shown to be bioactive. Aqueous extracts of leaves and unripe
fruit peel are known to have antifungal activity against
Colletotrichum gloeosporioides. The present study objective
was to describe the in vitro antifungal activity of n-hexane
extracts of mango harvest by products (leaves, seeds from
ripe and unripe fruit) and determine the minimum inhibitory
concentration of these extracts and qualitatively identify
some of the bioactive groups of compounds present in them.
A, PEEL
311
B, Latex
Mango fruits (cv. Neelum) at maturity were picked
with 5 cm long peduncle. The fruit surface and pedicel
were disinfected with 70% ethanol, and the fruit pedicel
was broken at the peduncle pedicel abscission point. Fruit
were inverted and the exuding latex was collected into a
glass vial. A drop of toluene was added to prevent microbial
growth. The latex was allowed to separate into an upper oily
and lower aqueous phase. The antifungal compounds in the
aqueous phase was extracted by n-hexane solvent infiltration
and concentrated in vacuo and used for further analysis.
Table. 1 In vitro testing of n-hexane extract against C. gloeosporioides and L. theobromae by poisoned food technique
Peel extract
C. gloeosporioides
L. theobromae
S.
(7 DAI)*
(5 DAI)*
Conc. (%)
No
Mycelial
Per cent
Mycelial
Per cent
growth inhibition over growth
inhibition
(cm)
control
(cm)
over control
0.40d
c
1
0.10
0.00
100.00
95.56
(3.67)
3.10c
2
0.05
0.00c
100.00
65.56
(10.15)
b
b
5.67
6.13
3
0.01
37.00
31.89
(13.77)
(14.33)
9.00a
9.00a
4
Control
(17.46)
(17.46)
Latex extract
C. gloeosporioides
L. theobromae
(7 DAI)*
(5 DAI)*
Mycelial
Per cent
Mycelial
Per cent
growth
inhibition
growth
inhibition
(cm)
over control
(cm)
over control
1.13d
c
0.00
100.00
87.44
(6.10)
1.89c
0.00c
100.00
79.00
(7.90)
b
b
4.78
2.36
46.89
73.78
(12.62)
(8.83)
9.00a
9.00a
(17.46)
(17.46)
RT
Peak
Area
(%)
S. No.
RT
Peak
Area
(%)
2.48
2,7,12,17-tetrabrom-(all-s)
cyclotetrathiophen(2,7,12,17tetrabromcycloocta[1,2-b:4,3-b':5,6b":8,7-b"']tetrathiophen
18.54
14
18.22
2-(Ethylenedioxy)
ethylamine,N-methyl-N-[4-(1pyrrolidinyl)-2-butynyl]
1.42
3.12
5"-(1,1-Dimethylethyl) 2,2',2",2"',
2""-pentamethoxy[1,1':3 ',1":
3",1''':3"'.1""-quinquephenyl]
3,3""-dimethanol
18.83
15
19.47
Dodecachloro-3,4benzophenanthrene
0.53
313
S.
No.
RT
Peak
Area
(%)
S. No.
RT
Peak
Area
(%)
6.55
2(3H)-Furanone, dihydro-4-hydroxy-
18.61
16
20.17
15-Bromo-4,4',12-tris(tbutyl)naphtho[1,2-f]
phenanthro[2,1-d]
0.53
8.31
15,31-Bis(dicyanomethylene)
-5,8,21,24-pentaoxa 2,11,18,
27tetrathiatricyclo[19(12,17)]
14.37
17
20.76
2-(tert-Butyl) -5-phenyl
dihydroisoxazolepentacarbonylchromium
0.51
9.56
N-Methoxy-N-ethylpropionamide
7.33
18
22.32
1.68
9.98
Methanol, triethylsilyl
7.33
19
24.54
2H-1-Benzopyran-4-ol,
3,4-dihydro-2-phenyl-
1.56
10.23
3,5-Diphenyl-2-(3',4'dimethoxyphenyl)-pyrrole
1.93
20
26.67
Spiro[N-Benzylpyrrolidin
-2-one-3,9'-xanthene]
1.56
12.43
Supinine
1.93
21
28.58
(Z)-[(Phenylthio)methyl]
1.56
13.26
Styrene-7,8-oxide
1.93
22
29.36
1,2-Bis(t-tributylsilyl)-1,2diphenylcyclotrisilan
0.94
10
13.87
0.68
23
31.28
Ejap-13
0.94
11
15.34
4-Phenyl-1-buten-3-yne
3.15
24
33.83
Lipo-3-episapelin A
0.94
12
16.78
10-Methyldodecan-5-olide
1.42
25
37.01
5,10-Dibutyltetra
benzoporphyrin
1.00
13
17.21
5-Cyclotetradecyn-2-one, 8-hydroxy14-penyl-1-oxa
1.42
26
41.19
3,4,5,6-Tetrahydro-7acetoxy-2-(1,3-dithian-2-yl)
2,6-methano-2H-1-benzoxocin
1.31
RT
3.22
6.98
Peak
Area
(%)
S.
No.
RT
2.99
15
26.08
Methyl -dgalactopyranoside
1.26
26.79
3-Methoxymethoxy3,7,16,20-tetramethylheneicosa-1,7,1
1,15,19-pentaene
1.31
2.99
314
16
Peak
Area
(%)
S.
No.
RT
Peak
Area
(%)
S.
No.
RT
8.65
4H-Pyran-4-one, 2,3-dihydro-3,5dihydroxy-6-methyl
2.68
17
31.34
-Cedrol
1.31
9.12
2,3-Dihydro-3,5-dihydroxy-6methyl-4H-pyran-4-one
2.68
18
31.76
7-epi-cis-sesquisabinene
hydrate
1.31
11.50
alpha-(2-(1,3,2methyldioxazano))
-isobutyric acid methyl ester
2.68
19
32.17
-D-Glucopyranose,
4-O--Dgalactopyranosyl
3.14
12.02
N-(1-Methoxycarbonyl-1methylethyl)-4-methyl-2-aza-1,3dioxane
2.68
20
32.69
Diethylvinylsilane
3.12
13.87
l-Alanyl-l-alanine ethylamide
2.68
21
33.43
2(3H)-Furanone,
5-ethoxydihydro-
3.14
15.08
9-Oxabicyclo[3.3.1]nonane-2,6diol
1.08
22
33.89
Betaxolol
2.18
17.55
-Pyrrolidone,
5-[3hydroxybutyl]-
1.08
23
35.20
Quinic acid
8.50
10
19.90
Pyrrolidine,1,2-dedihydro-5-[3acetoxy-1-butyl]-2-methylthio-
1.08
24
35.24
Desulphosinigrin
8.15
11
20.34
1.35
25
35.78
Phenol, 4-(3-hydroxy-1propenyl)-2-methoxy-
1.65
12
21.71
8-n-Hexyl-cis-7 thiabicyclo[4.3.0]
nonane
1.35
26
36.87
13
22.31
4-Azido-3-methylfuroxan
1.35
27
37.11
14
23.54
1.20
28
37.69
These results suggests that n-hexane extracts from
Mangifera indica L. cv. Neelum proved that the, antifungal
compounds in the peel and latex of mango is a potent
source for constitutive defence against Colletotrichum
gloeosporioides and Lasiodiplodia theobromae. Antifungal
compounds have previously been implicated for quiescence
on mango. This study revealed the presence of several
antifungal compounds in the unripe mango fruit peel and
latex. Antifungal activity was greater in immature fruits than
in a mature fruits.
REFERENCES
(+-)-2-Phenethanamine,
1-methyl-N-vanillyl
1-Methyl-8-phenyl-3,4dihydropyrrolo[1,2-a]
pyrazine
Doxepin
Peak
Area
(%)
1.65
1.19
2.02
315
316