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BIOTECHNOLOGY CHALLENGE
DEVELOPING A
SUSTAINABLE ENERGY
SOURCE THROUGH
ENTERPRISING R&D
Technical performance: Technological differentiation and compatibility with current equipment and
industrial processes.
Environmental performance: Reducing greenhouse gas emissions, combating deforestation and
soil erosion, and protecting the natural environment
and the water cycle.
Social acceptability: Compatibility with the food
needs of populations and a driver of economic and
social development in host country communities.
Economic viability.
CO 2
PHOTOSYNTHESIS
Sugar
beets
PLANT
SUGARS
AND
STARCH
BIOCHEMICAL
PATHWAY
(pages 8 and 9)
EXTRACTION
Sugar
cane
Grains
SUGARS
Wood
LIGNOCELLULOSIC
SUGARS
Farming
waste
(straw,
bagasse
and corn
stover)
Energy
plants
(miscanthus)
PHOTOTROPHIC
PATHWAY
(pages 10 and 11)
MICROALGAE
Microalgae
Amyris, Futurol(2)
BIOETHANOL
FERMENTATION USING
GENETICALLY MODIFIED
MICROORGANISMS
ISOBUTANOL
Gevo
ADDITIVES
GASOLINE
FARNESENE
BIO-JET FUEL
Amyris
LACTIC ACID
BIODIESEL
Futerro(3)
LUBRICANTS
OILS
PLASTICS
DNA Analysis
Molecular Biology
Screening
Researchers seek to obtain a stable microorganism (such as a yeast strain or bacteria) that
can produce the molecule of interest for chemical applications. To do that, they must fully
identify the cellular metabolic pathways to be able to reprogram the microorganism, to optimize
production of the target molecule, as illustrated in the diagram above.
Biodiesel
The biodiesel fuels on the market today consist primarily of vegetable-oil fatty acid methyl esters, which
have different properties than conventional diesel,
limiting them to 7% of the final fuels energy. Our goal
is to market biodiesel with higher biofuel content than
current blends.
In Brazil, Amyris has initiated tests on the largescale use of farnesane; its marketing affiliate has
been supplying the Sao Paulo transit authority
with biofuel containing 10% farnesane since
September 2011.
Around
6%
10%
(compared to 2% today)
Percentage of biofuel in the energy used
fortransportation worldwide in 2035 (1)
BIOCHEMICAL PATHWAY:
HARNESSING THE ENERGY OFSUGAR
The biochemical pathway uses microorganisms (yeast or bacteria) toferment
biomass and convert it to a variety of molecules usable for fuel and chemical
production. One of the research goals is to develop microorganisms that are
genetically modified to produce new molecules, then optimize them to obtain
efficient, robust strains.
Gevo: Total has acquired an interest in the U.S. startup Gevo, which is developing a process to convert
sugar to isobutanol, for use in fuels or petrochemical
production. The first commercial plant started up in
Luverne, Minnesota in May 2012.
EXTRACTING
LIGNOCELLULOSIC
SUGARS FROM
THEPLANT
Crop
Plant cells
Sugar molecules
Hemicellulose
Cellulose
Lignin
Sourcing feedstock is a major challenge for biochemical biomass conversion processes. To promote sustainability, Total is actively researching the use of lignocellulose, the non-edible part of plants. Lignin,
an extremely hard-to-break-down molecule, is a fibrous component that helps give plants their structure.
Expensive, energy-intensive processes are required to break it and allow hydrolysis of the cellulose and
hemicellulose to release sugars, such as glucose and xylose. Once extracted, the sugars can be fermented
into farnesene-type molecules (see Amyris, page 6) or bioethanol.
PHOTOTROPHIC PATHWAY:
FROM MICROALGAE
TOMOLECULES OF INTEREST
10
Carbon dioxide
CO2 fixing
Oxygen
O2
Photons
BIO-JET FUEL
BIODIESEL
OILS
Photosynthesis
Water
(H2O)
LIPIDS
RS
GA
PR
OT
SU
EIN
S
LUBRICANTS
Nutrients
(inorganic salts: iron, nitrogen, phosphate, etc.)
AMICROALGAE CELL
AlgaePARC
QIBEBT
Total launched a research program with Chinas
Qingdao Institute of Bioenergy and Bioprocess to
screen and identify enzymes that can be efficiently
used to produce biofuels and feedstock for green
chemicals.
11
12
GLOSSARY
Biofuel: A liquid or gaseous fuel for transport produced from biomass
(European Union Directive 2009/28/EC).
Biochemical conversion: Conversion of energy sources (usually
biomass) through biological transformation (reactions in living organisms).
Examples include fermentation (in the presence of enzymes).
Biodiesel: A biofuel used in diesel engines andproduced by the
conversion of vegetable oils, sugars orsynthetic gas.
Bioethanol: A biofuel miscible in gasoline produced by fermenting or
distilling sugars from biomass (sugar plants or lignocellulose).
Bio-jet fuel or biokerosene: Abiofuel for aviation use.
Biomass: Biodegradable fraction of products, waste and residues of
biological origin from agriculture (including plant and animal substances),
forestry and related industries, including fisheries and aquaculture which,
through chemical transformation, can become beneficial molecules
(carbon molecules) for the production of fuels and specialty chemicals
(European Union Directive 2009/28/EC).
Biotechnology: The application of science and technology to living
organisms, as well as parts, products and models thereof, to alter living
or non-living materials for the production of knowledge, goods and
services (OECD2005).
Distillation: The process of separating a liquid mixture through
vaporization. Substances vaporize at different boiling points, allowing
them to be collected separately.
Fermentation: A biochemical reaction that converts the chemical energy
in a carbon source (often sugar) to another form of energy (alcohol)
through interaction with yeast or bacteria.
Hydrolysis: The breakdown of a substance by splitting water molecules
into hydrogen (H+) and hydroxyl (OH-) ions.
Lignocellulose: Lignocellulose makes up the wall of plant cells. Inthe
biofuel sector, this term is used to designate wood and straw,
tworesources that can be used for biofuel production. Lignocellulose
can be gasified (thermochemical conversion) or split into its basic
components (sugars from cellulose and lignin) in order to transform them
through biochemical conversion.
Photosynthesis: The chlorophyll found in plants is capable of capturing
light energy. The captured energy is used to make sugar molecules from
water taken from the soil and carbon dioxide found in the air. The sugars
produced are then distributed through the plant and the oxygen is
released into the air.
Phototrophic organisms: Living organisms such as plants and
microalgae that make their organic matter by drawing energy from light
via photosynthesis.
13
New Energies
Total Marketing Services
Headquarters:
24 cours Michelet
92069 Paris La Dfense cedex - France
Phone: +33 (0)1 41 35 40 00
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www.total.com
Design and Production: Studios Menthe&Chocolat. Photo Credits: Total Photo Library. E. Maillard, M. Roussel, F. Laeuffer, Stephan
Gladieu, Marco Dufour All rights reserved to Fetranspor, A. Guillaumot/DPPI Media, Amyris, Inc. All Right Reserved, AIRBUS S.A.S. - photo
by exm company / P. MASCLET All rights reserved to AZUL Linhas Areas, iStockphoto, Getty Images (N. Rowe, Imagebroker RF, LWA), X,
All rights reserved. Graphics: StudioV2. Printed on Satimat Green, a paper manufactured from 60% recycled fi bers and 40% FSC-certifi ed virgin
fi bers. Printed in France. September 2014.
See you at
www.total.com