Beruflich Dokumente
Kultur Dokumente
H O S T E D BY
ORIGINIAL ARTICLE
a,*
, Mohamed M. El Shafei
Forensic Medicine & Clinical Toxicology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt
Diagnostic Radiology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt
KEYWORDS
Forensic anthropology;
Sex;
The 12th thoracic vertebra;
Lumbar vertebra;
Multi-slice computed
tomography;
Egyptian population
Abstract Introduction: Identication of the dismembered and skeletal remains has been a challenge for forensic anthropologists.
Aim: Therefore, the aim of the present study was to assess the sexual dimorphism from the 12th
thoracic and the rst lumbar vertebra measurements data obtained from reformatted images of
multi-slice computed tomography (MSCT) and to derive equations for sex determination in the
Egyptian population.
Subjects and methods: The study was conducted on 120 adult Egyptian patients (54 males and 66
females) with a mean age of 37.1 6.01. Twenty-four linear measurements were taken from the
12th thoracic and L1 vertebrae and then four ratios were calculated. The data was analyzed by
the statistical package for social sciences (SPSS) version 20. Accuracies and regression equations
for sex determination were then derived.
Results: Fourteen measurements out of the 24 linear measurements showed signicant sex differences when using the 12th thoracic vertebra. As regards to the rst lumbar vertebra, only seven linear measurements and one ratio of the posterior height of the vertebral body/anterior height of the
vertebral body (VBHp/VBHa) were sexually dimorphic. The percentage of accuracy of the 12th thoracic vertebra was found to be 93.1%, while that of the rst lumbar was 68.0%. When combining
the two vertebrae the accuracy increased to above 95% (96.3%).
Conclusion: Finally, it was concluded that the 12th thoracic vertebra is more accurate for sex
determination than the rst lumbar vertebra in the Egyptian population, which means that bone
dimensions are population specic.
2014 The International Association of Law and Forensic Sciences (IALFS). Production and hosting by
Elsevier B.V. All rights reserved.
* Corresponding author. Address: Department of Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Alexandria University,
Champollion Street, El-Khartoum Square, Azarita Medical Campus, Alexandria, Egypt. Tel.: +20 1001988116, +20 1002606166.
E-mail address: fatmabadreldine@yahoo.com (F.M.M. Badr El Dine).
Peer review under responsibility of The International Association of Law and Forensic Sciences (IALFS).
http://dx.doi.org/10.1016/j.ejfs.2014.07.005
2090-536X 2014 The International Association of Law and Forensic Sciences (IALFS). Production and hosting by Elsevier B.V. All rights
reserved.
Sex determination from the 12th thoracic and the rst lumbar vertebra measurement data
1. Introduction
Identication of the dismembered and skeletal remains has
been a challenge for forensic anthropologists especially in cases
of mass disaster and high intensity explosions, when severe
fragmentation, burning or commingling of human remains
has occurred.1
The determination of sex is an important starting point in
developing a biological prole for human skeletal remains as
it reduces the number of possible matches by 50%.2
Forensic scientists and physical anthropologists have traditionally employed morphological and anthropometric methods
for the estimation of sex from bone samples.3,4 The pelvis and
skull exhibiting prominent sexually dimorphic characters are
reliable indicators of sex estimation in cases where a complete
skeleton is available for identication. In the presence of complete skeleton, sex could be assessed with nearly 100% accuracy. This estimation rate is 98% for the pelvis and the skull
together and 95% for the pelvis only.3
Physical anthropologists are frequently asked to provide a
reliable estimation of the sex of unknown individuals represented by only a few bones or when several bones are missing
or are broken due to the effect of carnivores and environmental conditions. Therefore, exploration of sex differences in less
commonly used bones is also needed.5 Many previous studies
have attempted to determine sex by metric analysis of femur,6
humerus,7 patella,8 talus,9 calcaneus,10 metacarpals,11 metatarsals, tarsals,9 phalanges,11 clavicle,12 scapula13 and sternum.14
The rst cervical15 and the second cervical vertebrae can be
used to correctly classify sex with nearly the same level of
accuracy as the other traditionally used bones.16
In studies conducted by Pastor et al.17 and Yu et al.18 it was
found that the 12th thoracic and the rst lumbar vertebrae presented signicant sex differences and could be used in the
determination of sex from the fragmentary remains.
The vertebral column is comprised of a combination of
outer dense cortical bone and an inner cancellous bone that
assist in the weight bearing function of the vertebrae. In addition, the structural integrity of the vertebrae is maintained
throughout the taphonomic process.19,18 The 12th thoracic
vertebra is easily identiable in a disarticulated skeleton
because of its unique morphology. Its place as a transitional
vertebra results in the morphological characteristics between
both thoracic and lumbar vertebrae.20
Over the past decade, modern cross-sectional imaging techniques have revolutionized forensic medicine. Virtual anthropology obtained by the 3D imaging techniques such as
computer tomography (CT) and magnetic resonance imaging
(MRI) allow us to visualize almost every anatomical and
pathological structure with high resolution and quality.21
Magnetic resonance (MR) imaging and especially multi-slice
computed tomography (MSCT) are becoming more and more
widely used for post-mortem examinations.22
In certain cultural circles where a conventional autopsy is
stigmatized or even forbidden, a virtual autopsy would allow
sound medicolegal practice without violating religious
prohibitions or personal reservations.23
Despite the evidence of sexual dimorphism in the last thoracic and the rst lumbar vertebrae according to these previously published studies, the validity of these two bones for
sex determination has not been explored in the Egyptian
83
84
Table 1
Measurements
Code
Denition
EPDu
EPDl
EPWu
EPWl
VBHa
VBHp
FDs
FDc
PH
Pedicle width
PW
MPL
APH
ADm
TDm
SPH
SPL
Vertebral length
VL
Distance from the anterior edge of the endplate to the most concave point of the
posterior edge of the endplate. (Upper and lower surfaces)
Distance from the most left edge of the endplate to the most right edge of the
endplate. (Upper and lower surfaces)
Anterior height of vertebral body from left bisecting plane at the anterior part of
the vertebral body at the point, which can get the largest height
Posterior height of vertebral body from left bisecting plane at the posterior part
of the vertebral body at the point, which can get the largest height
Sagittal diameter of vertebral foramen on median plane
Coronal diameter of vertebral foramen on median plane
Height of right and left vertebral pedicle, from the superior border to the
inferior border
Width of vertebral pedicle (right and left) on superior plane, distance between
the two edges of the pedicle from a superior view
Length of mammillary process and pedicle; (right and left) distance from the
most posterior edge of the endplate to the most posterior edge of the
mammillary process
Height of superior and inferior left and right articular process from the vertebral
pedicles superior border to the most superior edge of the superior articular
process from a posterior view
Distance between articular processes from the most left to the most right from a
posterior view
Maximum distance between transverse processes from the most left edge of the
transverse to the most right edge
Height of spinal process from lateral view, from the superior border to the
inferior border at the posterior 1/3 point along anterior posterior axis
Length of spinal process on median plane, from posterior border of the
vertebral foramen to the most posterior edge of the spinal process
Sagittal length of the vertebra on superior or inferior plane, distance from the
anterior edge of vertebral body to the posterior edge of vertebral spinal process
Sex determination from the 12th thoracic and the rst lumbar vertebra measurement data
85
Figure 1 Reconstructed 3D images of the vertebra showing all the studied measurements. A and B, lateral view. C, inferior aspect. D
and E, superior aspects. F, anterior view. G, posterior view.
4. Discussion
Table 2 Descriptive statistics of the subjects by means of age
(N = 120).
Sex
Minimum
Maximum
Mean
SD
Male (n = 54)
Female (n = 66)
Total (n = 120)
26
27
26
45
44
45
38.11
36.09
37.1
6.04
5.85
6.01
86
Measurements
Measurements
EPDu
EPDl
EPWu
EPWl
VBHa
VBHp
FDs
FDc
PH right
PH left
PW right
PW left
APH right superior
APH left superior
APH right inferior
APH left inferior
ADm
TDm
MPL right
MPL left
SPH
SPL
VL superior
VL inferior
EPWu/EPDu
EPWl/EPDl
VBHp/VBHa
FDs/FDc
Male
Female
Mean
SD
Mean
SD
32.80
34.66
44.26
48.72
28.00
26.91
20.13
18.42
19.46
19.44
10.55
10.49
16.00
29.56
11.75
11.96
37.63
53.18
24.76
26.11
18.00
23.93
76.79
75.64
1.351
1.405
1.35
1.22
0.69
0.97
0.52
1.23
1.26
2.23
1.77
2.27
1.26
0.99
0.36
0.37
3.22
10.53
4.75
4.50
1.56
4.77
0.55
3.24
1.50
2.77
0.82
9.99
0.23
0.685
0.10
0.09
28.68
29.59
38.81
42.06
25.37
25.39
18.31
16.87
19.22
19.14
9.65
9.89
15.72
31.15
9.63
9.67
35.19
50.31
23.87
25.86
16.10
21.10
70.50
66.15
1.348
1.422
1.39
1.12
0.49
0.47
0.82
0.91
0.48
1.09
0.68
1.37
1.16
1.08
0.54
0.30
2.34
7.26
3.17
3.16
1.51
0.97
0.72
1.93
0.86
1.49
0.57
15.67
0.16
0.106
0.09
0.06
0.0001*
0.0001*
0.0001*
0.001*
0.001*
0.068
0.01*
0.01*
0.133
0.159
0.059
0.066
0.288
0.164
0.012*
0.001*
0.041*
0.058
0.071
0.084
0.01*
0.01*
0.0001*
0.0001*
0.22
0.336
0.061
0.225
To the best of my knowledge, there is no published literature on sex determination from anthropometric measurements
of the 12th thoracic and rst lumbar vertebrae among the
Egyp-tian population using Multi-Slice CT.
In the present study, the overall mean values collected from
the vertebrae of males are greater than that of females; this
indicates the presence of sexual dimorphism in the measurements of Egyptian vertebrae.
In the present study 14 measurements out of the 24 linear
measurements showed signicant sex differences when using
the 12th thoracic vertebra, and the predictive accuracy ranged
from 49.0% to 85.5%, with 3 variables having more than 80%
predictive accuracy lower end plate depth (EPDl), upper end
plate width (EPWu) and superior vertebral length (VLs), while
the articular process distance (APD) was the least accurate
(49%). In the study conducted by Yu et al.18 the predictive
accuracy ranged from 56.4% to 90.1%.
As regards the rst lumbar vertebra, only seven measurements and one ratio showed sexual dimorphism with a predictive
accuracy ranging from 47% to 79.0%. Only the upper end plate
depth (EPDu) showed an accuracy above 75% (79.0%). This is
lower than the reported results of Zheng et al.24 (57.186.6%).
Variations between results of different researches on different populations conrm the existence of population specic
differences exhibited in the vertebrae. Many factors such as,
EPDu
EPDl
EPWu
EPWl
VBHa
VBHp
FDs
FDc
PH right
PH left
PW right
PW left
APH right superior
APH left superior
APH right inferior
APH left inferior
ADm
TDm
MPL right
MPL left
SPH
SPL
VL superior
VL inferior
EPWu/EPDu
EPWl/EPDl
VBHp/VBHa
FDs/FDc
Male
Female
Mean
SD
Mean
SD
35.10
35.31
45.99
46.05
27.75
30.25
22.83
15.30
22.06
21.22
8.64
8.62
16.05
29.03
9.63
9.41
31.06
67.91
27.11
28.11
18.00
21.68
77.55
77.76
1.310
1.305
1.26
1.04
4.55
3.28
6.33
3.96
3.60
1.25
5.05
1.73
1.98
1.76
1.02
0.72
2.57
6.91
3.93
3.71
1.26
4.94
4.10
5.33
2.47
2.10
3.27
3.36
0.223
0.21
0.17
0.14
31.95
33.29
45.66
46.73
29.49
30.43
25.11
15.63
21.73
20.89
8.82
8.73
15.73
27.47
10.55
10.20
30.36
69.62
26.31
27.19
18.51
21.17
76.08
77.66
1.429
1.403
1.35
1.09
2.84
1.94
7.08
4.82
3.54
1.60
5.02
2.05
2.04
1.78
1.15
0.80
2.83
7.72
4.67
4.55
1.29
5.03
5.18
4.66
3.60
3.13
3.01
3.92
0.421
0.198
0.22
0.16
0.001*
0.001*
0.3931
0.2052
0.0043*
0.2425
0.0075*
0.1710
0.1860
0.1570
0.092
0.2262
0.2656
0.1249
0.1270
0.1532
0.0018*
0.0318*
0.256
0.278
0.1910
0.1526
0.005*
0.4419
0.652
0.225
0.011*
0.0805
Table 5 Cut off values (in mm) for sex differentiation using
the most signicant Egyptian individual variables in the 12th
thoracic vertebra.
Measurements
Cut o point
EPDu
EPDl
EPWu
EPWl
VBHa
FDs
FDc
APH Right inferior
APH Left inferior
APD
SPH
SPL
VL superior
VL inferior
Female
Female
Female
Female
Female
Female
Female
Female
Female
Female
Female
Female
Female
Female
<30.5<
<31.5<
<42.6<
<46.2<
<26.5<
<19.0<
<17.1<
<10.6<
<10.5<
<36.1<
<17.1<
<22.1<
<74.5<
<70.0<
Male
Male
Male
Male
Male
Male
Male
Male
Male
Male
Male
Male
Male
Male
Sex determination from the 12th thoracic and the rst lumbar vertebra measurement data
Table 6 Cut off values (in mm) for sex differentiation using
the most signicant Egyptian individual variables in L1
vertebrae.
Measurements
Cut o point
EPDu
EPDl
VBHa
FDs
APD
TDm
VL sup
VBHp/VBHa
Female
Female
Female
Female
Female
Female
Female
Female
<33.5<
<34.1<
<28.1<
<23.5<
<30.8<
<68.1<
<75.3<
<1.28<
Male
Male
Male
Male
Male
Male
Male
Male
Unstandardized
coecient
EPDu
EPDl
EPWu
EPWl
VBHa
FDs
FDc
APH right inferior
APH left inferior
APD
SPH
SPL
VL superior
VL inferior
.018
.129
.049
.157
.018
.0154
.053
.039
.114
.022
.305
.142
.007
.006
Accuracy (%)
Male
Female
Total
75.0
85.5
82.5
78.5
76.8
62.5
69.8
61.8
79.8
42.6
74.2
74.5
80.0
75.8
80.5
85.0
85.0
75.5
77.5
66.1
64.5
60.1
80.2
52.1
77.5
72.5
82.5
77.1
78.5
85.0
84.0
76.0
76.9
65.0
68.0
61.0
80.0
49.0
76.0
73.0
82.0
76.0
Unstandardized
coecient
Accuracy (%)
Male
Female
Total
EPDu
EPDl
VBHa
FDs
APD
TDm
VL sup
VBHp/VBHa
.040
.008
.031
.031
.087
0.034
.346
.005
78.5
75.2
58.8
68.9
52.5
45.5
65.5
65.1
80.1
74.6
62.1
72.8
55.0
48.5
66.2
67.2
79.0
75.0
60.0
70.6
53.0
47.0
65.0
66.0
Table 9
87
In the study conducted by Zheng et al.24 on the Chinese population; 25 out of 34 traits were sexually dimorphic with 5 variables having a predictive accuracy more than 80%. These different results may be due to the difference in the sample size.
The sex was correctly assigned using cut off values for the
most signicant measurements of 12th thoracic and the rst
lumbar vertebrae. This study has shown that measurements
of the vertebrae of Egyptians are sexually dimorphic. The level
of accuracy obtained from the use of 12th thoracic vertebra by
regression analysis was 93.1%. This result is similar to that of
Yu et al.18
On the other hand, the level of accuracy obtained from L1
was 68.0%, this is much lower than that obtained by Zheng
et al.24 (88.6%). This could be explained by the difference in
sample size and the number of measurements used.
By a combination of the 12th thoracic and L1 vertebrae,
only ve variables occurred in the equation that predicted
sex with a high level of accuracy (96.3%).
The level of accuracy provided by the 12th thoracic vertebra alone is near that obtained by the 12th thoracic & L1
combined; this means that the 12th thoracic vertebra could
be used as a single bone for sex determination especially in
cases of fragmentary forensic assemblages.
5. Conclusions
In conclusion, the sexual dimorphism is present in different
regions of the vertebral column. The accuracy of its assessment
can vary among populations. A large number of variables were
assessed to counteract the problems related to damage of the
bone material. It was observed that the 12th thoracic vertebra
provides a better determination of sex among Egyptians than
the rst lumbar vertebra. So the rst lumbar vertebra should
not be used alone in sex estimation in forensic caseworks.
However, the percentage accuracy increases when the two vertebrae are used together which encourages the use of these
parameters and equations as predictors in sex determination
problems.
6. Recommendations
Further researches for Egyptians must be done using a larger number of subjects with more age groups.
Further researches for other populations using CT for vertebrae are recommended.
The current study has used only the last thoracic and the
rst lumbar vertebrae for sex determination; therefore more
studies should be done on other vertebrae.
Accurate and reliable modern imaging techniques such as
MSCT should pioneer postmortem investigations.
Formulae for sex determination based on multiple regression analysis of vertebral measurements.
Vertebra used
Equation
Accuracy
(%)
Y = 13.156 (0.187 VL sup) (0.10 EPDl) + (0.092 EPWl) + (0.003 VL inf) + (0.025 APD)
Y = 4.503 (0.043 VL superior) (0.023 EPDl) + (0.040 VBHa)
Y = 13.156 (0.187 VL sup) (1.0 EPDl) + (0.092 EPWl) + (0.003 VL inf) + (0.025 APD)
93.1
68.0
96.3
88
Table 10
Authors
Population
Vertebrae
Research subjects
Number of sample
Accuracy
Present study
Egyptian
CT
120
Marino (1995)25
Wescott (2000)5
Yu et al. (2008)18
Marlow et al. (2011)16
Zheng et al. (2012)24
Yu et al. (2012)26
Bethard (2013)27
American
American
Korean
UK
Chinese
Chinese
American
The
The
The
The
The
The
The
Real
Real
CT
Real
CT
CT
Real
Funding
None.
Conict of interest
None declared.
Informed consent
The Ethics Committee of Alexandria Faculty of Medicine
approved this study, and all patients included signed an
informed consent document.
Ethical approval
The Ethics committee of Faculty of Medicine, Alexandria University, for human experimentation, approved this work
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