Enzymes for Citrus

Processing
Novozymes
Customer Solutions
Status Nov 2012

Slide No. 2

Main Applications in Citrus Processing

Application

Benefit to customer

Enzyme used

Essiental Oil recovery *

Higher oil yield

Less contaminated waste water

NZ 33095 or Pectinex Utra SP-L

Pulp/Core wash (cloudy juice)

Easy and fast realase of juice

Higher yield

Pectinex Ultra Mash (non GMO) or

Pectinex Smash XXL

Peel extract/Natural cloudifier *

Peels (waste) can be used for by products

Less waste

Ultrazym AFP- L

Viscosity reduction

Avoid jellification
Higher concentration fold is possible

Pectinex Ultra Clear or Pectinex XXL

Clarification

Fast sedimentation
Easy to filter

Pectinex Ultra Clear or Pectinex XXL

Peeling

Highest standard of peeld fruits

Reduced manual labor costs

Pectinex Ultra SP-L or Viscozyme

* by products

Slide No. 3

The Use of Enzymes during Citrus Juice Processing

Essential Oil Extraction

Pulp-Wash/Core Wash
Cloudy Peel Extract
Viscocity reduction

Fruit
Juice

Juice

Pro-

Pulp-Wash
max 10%
Juice-Sacs
Cloudyfier
Peel-Pellets

Citrozym
Cloudy

By-

Solution

ducts
Water-Oil

+
E
+
Peel/Core

Fresh

20%

Pulp

Separators
Sludge

Water
20%

Oil

Slide No. 4

Recovery of Essential Oil

Oil Extraction

Citrozym CEO

Finisher

(or NZ 33095)

Citrozym CEO
(or NZ 33095)

Oil-water Emulsion

SOLIDS
SLUDGE

1st centrifuge
Oil-phase
Buffertank

SOLIDS
SLUDGE

centrifuge

2nd centrifuge

Essential Oil

Citrozym CEO
(or NZ 33095)

Slide No. 5

Advantages using enzyme during Recovery of Essential

Oil (1)
Higher yield due to easier emulsion breaking

improved oil recovery

10-15% more yield

optimise the efficiency of the centrifuges

Excellent oilquality

Lower water consumption, and less contaminated

waste water

reduction of fresh water consumption

Wast water easy to dump, due to lower BOD

no investments (only holding tank)

Slide No. 6

Essential Oil Extraction (2)

Oil yield lb./mt fruit
9.2

Citrozym CEO
(or NZ 33095)

8.7
8.2

3.77 kg/mt
7.7

(8.3
7.2

lb./mt)

control

6.7

3.27 kg/mt
Industrial trials on oranges,

6.2

150,000 boxes/day, Brazil

5.7
1

trial days

10 11 av

(7.2 lb./mt)

Result from an industrial trial

in Brazil during 2
weeks, processing 6000 tons
of oranges/day,
yielding +0,5 kg of oil/ton of
fruit.

Slide No. 7

Benefits from Citrozym CEO (or NZ 33095)

on essential oil extraction (3)

Parameters

Control

Citrozym CEO
(or NZ 33095)

Yield of oil
in kg/ton of fruits

3.27

3.77 (15% increase!)

Efficiency of centrifuges
(yield/capacity) in %

50

72

Efficiency of polishing centrifuges

(yield/capacity) in %

83

88

Water consumption
Status of waste water

minus 35%
Difficult to bio-degrade

Easy to bio-degrade

Enzyme cost: 100ppm Citrozym CEO (or NZ 33095)

1200 lt water/hr (water is recicled and needs a correction with 15% additional enzyme per hr)
1 FMC makes 3.8 tons of oranges/hr

Slide No. 8

Production of PW/CW/WESOS
Juice extraction
Finisher
pulp

juice

scroll mixers
several
Finisher

Enzymation

Citrozym Cloudy (or Pectinex SMASH XXL)

5-20 ppm / 45C / ~45 min

Pasteurization
Centrifugation
Concentration
PW concentrate
60Bx

Slide No. 9

Pulp & Core Wash or WESOS

Enzyme used for:

In-line or off-line PW/CW

Enzymes for extraction and/or viscosity reduction
limiting production of Gal.Acid
no separation and avoid clarification
inactivation with pasteurisation

Parameters for the Enzymation

Citrozym Cloudy (or Pectinex SMASH XXL or Ultrazym AFP-L)

5 - 15 ppm for Pulp Wash

10 - 20 ppm for Core Wash


45C


40 min

Slide No. 10

Production of Natural Cloudy Peel

Products Cloudyfier

Peel of
Orange, Lemon
or Grapefruit

Milling
Water addition
water : peel = 1:1
ev. pasteurization
Citrozym Cloudy (or Pectinex SMASH XXL
20 - 30 ppm

Cloudy peel products are

obtained from citrus
peels. They are
extracted by water and
Citrozyme cloudy (or
Pectinex SMASH XXL)
and afterwards
concentrated to approx
50Bx. Natural
cloudyfier are used as
basics for cloudy soft
drinks industry as as
they are extreme cloud
stable.

Enzymation
1 hour, 45C
stirring

Separation
Decanter
Citrozym Cloudy (or Pectinex SMASH XXL)
10 - 30 ppm

Enzymation
30 - 60 min, 45C

Pasteurization

Centrifugation

Concentration

Cloudyfier 50 BX
Natural Cloudifier

Viscosity reduction orange juice (Navels)

Slide No. 11

Oranges
Juice extraction

Finisher

Enzymation tank

Pasteurization
Centrifugation
Concentration
Cloudy 65 Bx
OJ Concentrate

Citrozym Cloudy (or Pectinex Ultra Clear)

5-20 ppm
20- 45 C, 15 - 30 min.

Slide No. 12

Clarification of lemon/lime juice

Lemons
Limes
Juice
Extraction

Finisher

Enzymation
tank

Citrozym Ultra L
(or Pectinex Ultra CLear)
30 - 70 ppm
15 - 40 C
3 - 8 hours

Clarification
silica sol
Centrifugation/
trad. Filtration
Pasteurization
Concentration
Clear
LJ Concentrate

UF

Slide No. 13

Clarification of lemon and lime juice

fresh juice or past.& rediluted
pH (1.8) 2.3 - 2.9
ambient temp. (max. 45C)
as lower the pH, as lower temp.
has to be used

3 - 6 hours

trad. filtration or UF

Juice pectin negative and stable

Good filterability
Low arabian content

Citrozym Ultra L (or Pectinex

Ultra CLear)
for production of clear lemon
concentrate

Citrozym Ultra L (or NZ 33095)

Slide No. 14

Slide No. 15

A closer Look at the Technologies

Whole Peeled Fruit & Fresh-Cut Fruit Segments :
Orange & Grapefruit using Vacuum Infusion

Pectinex
Ultra SP-L

Slide No. 16

Whats it like by enzymatic peeling

Pectinex Ultra SP-L results in:

- Highest possible quality
- Reduced losses during
processing
- Less manual labor costs

Citrus fruit processing

Reduced viscosity and avoidance of problems in the
concentration (jellification of concentrated cloudy
material)

Improved extraction of solids

Maintained cloud stability, if necessary

The new NZ 33095 for citrus processing

Due to its robustness (lowest pH, low and high
temperatures) extremly suitable for lemon
clarification

More and stronger balanced enzyme activities and
better heat stability even at 60 C (10-60 C)

Better than competitor non gmo derived enzymes

Suitable for organic production

Suitable for oil extraction

Product range Juice 2010

Pectinex YieldMASH, Pectinex XXL, Amylase AG XXL our
preferred choice together with Pectinex UF for highest yields and
capacities and clear stable juices and concentrates

Pectinex Ultra MASH, Pectinex Ultra Clear (NZ 33105), Amylase
AG 300 L and Pectinex UF for good yield and capacity and clear
and stable juices and concentrates

Several other enzymes, e.g. Pectine Ultra AFP (NZ 33109),
Pectinex Ultra SP-L, Ultrazym AFP-L, Pectinex BE XXL and
Pectinex Ultra Colour (NZ 33103) to full fill your needs

20

02/05/2013

NOVOZYMES PRESENTATION

We find the magic of nature in a handful of soil or a

compost heap. Then we turn it into solutions for
detergents, pharmaceutical products, the energy sector and
countless other applications.

Novozymes is the world leader in bioinnovation.

Together with our partners and with juice producers
around the world we create tomorrows industrial
biosolutions, improving our customers business
and the use of our planets resources. Read more
at www.novozymes.com/juice
fruitjuice@novozymes.com,

THANK YOU FOR

YOUR ATTENTION