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Constructed wetland treatment systems offer a practical alternative to the chemical treatment of drainage
from certain coal-related sources (Brodie et al., 1989;
Nu-Hoai et al., 1998). The advantages include low costs
of construction, operation, and maintenance, with effective removal of many contaminants such as heavy metals
and metalloids (National Rivers Authority, 1992; NuHoai et al., 1998). In addition, the systems are usually
better able to cope with fluctuating water flow rate and
variable contaminant concentrations than conventional
treatment systems (Bastian and Hammer, 1993). The
success of wetlands has resulted in the construction of
more than 400 wetlands in the USA for the purpose of
treating drainage water from coal mines alone (Perry
and Kleinmann, 1991).
Wetlands are capable of removing large quantities of
trace elements from wastewater passing through them.
However, there is considerable variation among metals,
and also between wetlands, in the degree to which each
metal is removed by the wetland vegetation. For example, in a review of constructed wetlands in the USA,
Skousen et al. (1994) reported that the removal of iron
(Fe) from acid mine drainage varied widely between 28
and 99%. In the case of manganese (Mn), where the
range of removal rates was between 8 and 98%, the
mean removal rate was actually less than 30%. The
mechanisms of removal for each contaminant from
wastewater, and the interrelatedness of these different
mechanisms, are not well understood (Faulkner and
Richardson, 1989; National Rivers Authority, 1992). It
is thought that the cleanup of water is accomplished
through a variety of physical, chemical, and biological
processes operating independently in some situations,
and interactively in others (Dunbabin and Bowmer,
1992; Hammer, 1993).
Physicochemical mechanisms of metal retention in
the sediments include immobilization via oxidative precipitation, sedimentation of metal-laden particulates,
adsorption on cation exchange sites, complexation with
organic matter, and sulfate reduction (Wieder, 1988;
Perry and Kleinmann, 1991; Brodie et al., 1993). Precipitation of metal oxides, following microbially mediated
oxidation, is thought to be one of the most important
removal mechanisms in wetlands (National Rivers Authority, 1992; Skousen et al., 1994). Indeed, 40 to 70%
of the total iron removed from acid mine drainage by
some wetlands was found as ferric hydroxides from the
hydrolysis of ferric iron or from microbial oxidation of
ferrous iron (Henrot and Wieder, 1990).
Abbreviations: APS, Allegheny Power Services; EC, electrical conductivity; DO, dissolved oxygen; NPDES, National Pollution Discharge Elimination System.
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Fig. 1. The Allegheny Power Services (APS) treatment system for coal combustion by-product leachate.
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Water Sampling
Influent and effluent waters were sampled quarterly from
May 1996 through May 1997, and then monthly from July
1997 to June 1998. The pH, temperature, electrical conductivity (EC), and dissolved oxygen (DO) content of the surface
water of each cell were measured in situ at the wetland using
a Corning Checkmate Modular System (Fisher Scientific,
Pittsburgh, PA). Water samples for trace element analysis
were collected from the inlet of Cell 1 and from the outlet of
Cell 4 of the vegetated cells. In March, April, and June 1998,
water samples were also collected from the outlet of Cells 1,
2, and 3. In each case, three replicate 250-mL grab samples
were collected using the bottle submersion method (Byrnes,
1994), and acidified immediately with 1 mL of concentrated
nitric acid. All samples were transported to the laboratory at
4C for chemical analysis.
Samples of pore water in the 0- to 10-cm sediment layer
were collected at the same time as the influent and effluent
waters. Pore water was collected from the same quadrats as
the vegetation sample, using 0.1-m Rhizon soil moisture filters (Ben Meadows, Canton, GA) connected to 20-mL Vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ), which
were allowed to fill to capacity. This method provided a sample
from the composite 0- to 10-cm sediment profile. The sample
was transported to the laboratory at 4C for chemical analysis.
Plant Sampling
Plant samples were collected on a monthly basis from May
1996 through June 1998. Plant species composition and plant
species density were sampled in three randomly selected 1-m2
quadrats in each vegetated cell. Three replicate whole-plant
Sediment Sampling
Sediments were sampled quarterly over the first year (May
1996 to May 1997), and an additional collection was made at
the end of the study (November 1998). Three replicate sediment samples were collected from the top 15 cm of sediment
in each cell using an AMS (American Falls, ID) sediment
recovery probe with replaceable 5- 25-cm butyrate liners.
The sediments were transported to the laboratory in the
capped liners at 4C. Sediments were then removed, cut into
three 5-cm profile sections, air-dried, and ground to a fine
powder.
Chemical Analysis
All samples were analyzed for total trace elements. Water
samples were digested with HNO3, H2O2, and HCl according
to USEPA Method 3010A (USEPA, 1992). Trace element
concentrations were determined by inductively coupled
plasma atomic emission spectrometry (ICPAES). Elemental
analysis of plants was carried out by acid digestion of dry
samples (Zarcinas et al., 1987), followed by measurement of
total concentrations of all elements of interest in the acid
digest using ICPAES. Sediment samples were acid digested
with HNO3, H2O2, and HCl using USEPA Method 3050B
(USEPA, 1996) before chemical analysis by ICPAES.
Statistical Analysis
Sample means and standard errors were determined from
the replicate water, plant, and sediment samples. The data
were analyzed statistically using one-way analysis of variance
(ANOVA); significant differences between individual means
were determined using the Tukey multiple comparisons test
(Zar, 1984).
RESULTS
Physical Characteristics of Surface Water
There were no significant differences (P 0.05) between the four cells in the pH, EC, DO, and temperature
of the surface water. The values are therefore averaged
over the four cells (Fig. 2). Over the 26-mo study period,
the pH of the surface water averaged 7.2, and ranged
from 6.2 to 7.7. The average EC was 2.2 mS cm1, ranging from 1.9 to 3.0 mS cm1. There was some seasonal
variation in the DO concentration in the surface water,
with concentrations ranging from 5 to 14 mg L1. The
lowest values were in the summer (July through September), and the highest in the winter. The water temperature varied greatly from 0 to 30C during a year. The
DO and temperature were inversely proportional; high
temperatures resulted in the lowest concentrations of
DO (Fig. 2). The lowest DO values were most likely
due to the lower solubility of oxygen in summer and to
the greatest microbial activities when water temperature
was high.
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Fig. 2. Temporal changes in the surface water pH, electrical conductivity (EC), dissolved oxygen (DO), and temperature. Values are
averaged over the four vegetated cells for each month. Error bars
indicate standard errors (SE). Dashed horizontal line denotes overall average.
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Fig. 3. Temporal changes in the concentration of Fe, Mn, Co, and Ni in the inlet water to Cell 1 (hatched bars), and the outlet water from Cell
4 (filled bars). Values are means plus SE, n 3.
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Fig. 4. Concentrations of Fe, Mn, Co, and Ni in the inlet water to Cell 1 and the outlet water from Cells 1, 2, 3, and 4 in 1998. Solid line denotes
the percentage reduction in concentration. Values are means plus SE, n 3.
DISCUSSION
Metal Removal Efficiency of the Vegetated Cells
The water flowing into Cell 1 of the vegetated part
of the wetland contained Fe and Mn concentrations
above the 1996 NPDES permissible limits. Previous
studies showed that constructed wetlands are effective
in removing Fe from metal-polluted waters, but are less
successful in removing Mn (Stillings et al., 1988; Fennessy and Mitsch, 1989; Hedin, 1989; Skousen et al.,
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Table 1. Metal concentrations (mg kg1 ) at different depths in the sediment profile. Data are means (SE, n 6) of samples collected
from all four cells between May 1996 and November 1998. Differences between depths were determined by analysis of variance
(ANOVA); letter codes indicate significant differences (Tukey multiple comparisons test, P 0.05).
Metal
Fe
Mn
Co
Ni
Depth
cm
05
510
1015
05
510
1015
05
510
1015
05
510
1015
Cell 1
Cell 2
Cell 3
Cell 4
mg kg1
35 200 1 900
31 300 1 500
31 300 1 100
3 310 1 080a
1 390 270b
1 080 80b
31 5a
18 1b
16 1b
59 11a
37 3b
36 3b
31 300
32 000
31 600
1 350
960
890
21
16
16
45
38
37
1 100
1 500
800
170
100
40
2
1
1
4
3
2
32 600 1 000
31 800 1 400
32 500 1 000
830 71
832 51
859 42
17 1
16 1
16 1
40 2
39 2
39 2
32 600
33 300
32 100
872
856
892
17
17
16
42
41
37
1 200
900
1 200
75
74
76
1
1
1
2.5
3.0
1.4
Fig. 5. Concentrations of Fe, Mn, Co, and Ni in the top 15 cm of sediment in four vegetated wetland cells. The gray bars show the concentration
of each element in the substrate before the water treatment began (data from Electric Power Research Institute, 1998). Values are monthly
averages of all four cells plus SE, n 4.
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Cell 1
Cell 2
Cell 3
Cell 4
mg kg1
Cattail fallen litter (n 10)
Cell 1
Cell 2
Cell 3
Cell 4
kg1
Fe
Mn
Co
Ni
268
2 010
0.76
2.0
47
170
0.18a
0.2
mg
Aboveground tissues
178 26
222 58
2 350 230
2 440 230
0.33 0.07b
0.32 0.06b
1.9 0.2
2.4 0.4
Fe
Mn
Co
Ni
7 440
1 650
11
16
Belowground tissues
6 430 520
7 320 760
915 84
1 300 180
4.0 0.7b
3.9 0.6b
9.6 1.3
12 2
805
170
1.7a
2
218 48
2 060 180
0.28 0.04b
2.2 0.3
8 000
940
3.1
11
1 470
180
0.5b
2
Fe
Mn
Co
Ni
7 010
11 800
94.6
94.3
1 120a
1 800a
27.2a
19.7a
Fe
Mn
Co
Ni
36 200 13 700a
23 900 3 560a
325 92a
299 92a
1 690
6 800
24.3
47.7
Chara (n 8)
1 310
4 050
3.3
38.7
180b
530c
1.1c
7.2b
9 280 1 760b
11 900 2 170b
14 5b
66 26b
Mn
Co
Ni
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