Beruflich Dokumente
Kultur Dokumente
Marsh,
Deborah 1. Jacobs,
and Hans Veening
Bucknell University
Lewisburg, Pennsylvania 17837
rk!!,
1.5V
Batt.
Switch
SPST
E
25K ohm
DPDT
Switch
Pt Indicator
Electrodes
The excess bromine is then converted to iodine by reaction witb potassium iodide. Finally, the iodine (triiodide)
produced by this reaction is titrated with standard thiosulate using starch indicator.
The unknown samples issued to the students consisted
of commercially available vitamin C tablets with ascorbic
acid levels ranging from 100-500 mg per tablet. The unknown tablet is weighed to the nearest *0.1 mg and
transferred to a 250.0-ml volumetric flask, dissolved in approximately 100 ml of distilled water (dissolution takes
about one-half hour), diluted to volume and mixed. The
resulting solution will be somewhat cloudy due to the
presence of inert substances in the tablet. The solution
must he kept out of the light as much as possible and
must be titrated within 2 hr or significant deterioration
will result.
Coulometric Titration of Ascorbic Acid at Constant
Current
The determination of ascorbic acid is carried out in this
part of the experiment by using a slightly modified version of the coulometric procedure reported by Evans (11).
The apparatus includes a constant current amperostat of
the type described by Vincent and Ward (12). A milliammeter was used to monitor the curr?nt through the generator electrodes. The titration flask and the generating and
indicator electrodes were identical to those previously described (11). The amperometric end point detection sysPresented at the 165th National Meeting of the American Chemical Society, Dallas, Texas, April, 1973.
~~
OH
HO-CH,-CH-C.
H
I/\/O
.C
Br;
ascorbic acid
dehydraascorbic acid
Reagents and Procedure
me Ascorbic
A G in~sample (Manu- mg Ascorbic Number
of
facturer's
Acid
Found
Trials
Label)
curve
Sam&
Rexall Chewable
Vitamin C Tablets
(Lot No. K12003)
Squibb Ascorbic Acid
Tablets (U.S.P.)
(Control No. 037223
Purepac Vitamin C
Tablets
(Lot No. 0170K3)
Squibb Ascorbic Acid
Tablets (U.S.P.)
(Control No. 0L561)
Purepae Vitamin C
Tablets
(Lot No. 0167K3)
C. P. Reagent Grade
Ascorbic Acid
(Eastman)
100
101f5
100
108 f 2
250
255 + 6
250
259 f 6
500
499 f 2
562
563 f 1
504
469
504 + 1
468 1
3
3
Sample
Squibb Ascorbic Acid
Tablets (U.S.P.)
(Control No. 0B695)
Squibb Ascorbic Acid
Tablets (U.S.P.)
(Control No. 0E722)
Squibb Ascorbic Acid
Tablets (U.S.P.)
(Control No. 0L567)
Purepae Vitamin C
Tablets
(Lot No. 0167K3)
mg Ascorbic
Acid in samole (Manu- ma Ascorbic Number
'faiturer's
- Acid
of
Label)
Found
Trials
50
50.4 + 1
100
101 + 1
250
255 -L. 4
500
498 + 2