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The differing baits analyzed are classified based on the preparation material and the
found substance.
Methods and materials.
All the data presented in this work were extracted from a total of received cases in our
laboratory that include suspicious animals died of food poisoning and potential
poisoned baits found in the environment.
As prescribed by law, an adequate follow-up of the process must be carried out in field
by Environmental Partners of the regional government or by SEPRONA( Protection of
Nature Service).
Subsequently, they were forwarded to the establi.shed entity, the Recovery Center of
Wildlife Los Hornos located in Sierra de Fuentes (Cceres), where specialized
personnel would evaluate each of the cases.
In the event of suspicious evidence of malicious poisoning, specialized personnel
concerned would provide us relevant samples along with a Record of Delivery (Acta de
Entrega) in which all details involved were outlined.
Once all samples were received at Toxicology Laboratory of the Veterinary Faculty of
Cceres), all data related to it were registered (assigned number of register, specie, data
and collection point).
Each of the samples were visually examined and were provided of attached information
containing details of every particular case. This would ascertain the suitable
toxicological analysis.
If visual examination and data seemed to suggest the opposite, analysis of
Anticholynesterasics compounds were routinely carried out over samples, as they are
often involved in intentional poisoning due to its simple handling, especially in granular
samples.[9].
For this analysis organic solvents extractions and purification processes were performed
that have varied over time from liquid-liquid or solid phase extraction cartridge to gel
permeation chromatography using the standardized methodology of Stahr[10].
If toxic material was applied on the skin surface of the bait, and made manual removing
possible, then it would be diluted in the appropriate solvent to its further analysis.
The obtained extracts would give a clear idea of quantification and determination of
toxics by employing diverse chromatographic techniques that are also varying over
time:
-Thin Layer Chromatography (TLC): used as an initial phase (screening), after
applying the sample on silica-gel plates and it reveals using butyrylcholinesterase
(BuChE) through Zoun and slightly modified Spierenburg.[11].
-High Resolution LiquidChromatography (HPLC) along with Diode Array(DAD)
detector.
-Gas Chromatography with spectrometry mass detector.( CG-MS) (Shimadzu GCMSQP2010 Plus) used since 2007.
In the event of any suspicion regarding to involvement of any other toxic, strychnine
and metaldehyde methodology described by Saldaa was specifically applied on
them (1981) [12].
Results
For the most part analyzed baits were constituted by a meat component (69% of the
cases studied). animal carcasses with remains of poison left behind could have
been sprayed with these chemicals ( in some cases they were visible at a
glance(Fig.1 ) or effect upon the animal organism would cause death leaving
remains of the living organism.
23 out of 80 cases, granular cells were visible at a glance(fig 1). In 9 cases where
substances in its commercial form were found (4 micro-granules cases, 2 were
rodenticide, and 3 were covered seeds).They were strongly associated to fauna
poisoning or preprepared baits from nearby areas.
In this study, anticholinesterasic compounds, especially granular cells (aldicarb and
carbofurano), were revealed as the most used substances for these kind of
malpractices (see table n1). In 6 cases combination of different compounds
showed up in a single bait.
One corresponded to 3 meat baits impregnated with chlorpyrifos, Methomyl, and
endosulfan; 2 corresponded to poorly buried covered seeds
(metiocarb+imidacloprid+metalaxil+thiram+atrazine), another was a white putty
wrapping a Malathion, demetn-o-metil and carbaryl; in the latter case, a commercial
anticoagulant rodenticide was found in the forms of blueish cereal grains in which the
presence of brodifacoum and difenacoum was detected.
Discussion.
The high percentage of meat baits makes clear that target species are carnivorous
mammals, birds of prey and scavengers;
These birds flyover a large sensible area where extensive farming prevails and where
poisons haven been traditionally used to avoid vermin attacks against livestock and
game animals.
Birds would eventually ingest the bait or consume carcasses of other victims.
Extensive using of meat baits in such high proportion points out its low economic cost,
easy management, and resistance to degradation when faced to weather
conditions(especially when it comes to sausages(cold meat).
There was no direct connection between the used fuel( higher fat content, elaborated
food or raw, etc.) and the wrapped substance content, unlike other similar studies[13].
Proof of this can be seen in data obtained in this study. This conflict may become more
notorious in areas where important game animal activity, extensive livestock, and fauna
richness of birds of prey and carnivorous mammals merge into a common area .
Therefore, some authors considerate old persecution regulations of predators ensue
terrible consequences that harm fauna conservation to this day.
In Spain 1/1989 law from march 27th, of the natural spaces conversation and fauna and
flora , stated prohibition in its 34th article, the holding, commercialization, and usage of
poisons to kill animals. After that, 10/1995 Organic Law from November 23rd [24],
stated in its 336th article of the Penal Code, the penalization in using poisons to either
hunting or fishing, as a non-selective medium.
The 5/2010 organic law reform adds the non-selective practice term. Likewise,
42/2007 Natural heritage and biodiversity law[26] in 62.3 article stablishes:
a) prohibition of all massive procedures or non-selective to capture or kill animals.
Use of poisons against fauna is an offence defined in the penal code to this day.
Subsequently, different regions have legislations in process intended to these
malpractices; particularly, in Extremadura, it is regulated by 8/1998 law from june 26th
of Conservation of Nature and Natural Spaces of Extremadura (art.66)[27] and 14/2010
law, from December 9th , of hunting in Extremadura(art. 35 and 87) [28].
Furthermore, this legal framework comes along with strategies and plans that at an
autonomical and national level have developed measures to prevent and prosecute this
offence.
Precisely, the estrategia extremea(extremeos strategy) to eradicate poisons usage in
nature, follows 5 crucial lineas that consists of: increasing and information flowing
readily , improvement of knowledge, development and executing of specific operations
intended to prevention and deterrence, research and prosecution of the offense, criminal
and administrative proceedings, as well as other complementary actions .[29].
A highly debatable topic during the latest years has been the use of poisons to pest
control,as the plague of voles that took place in Castilla y Len, mitigation measures
were applied making use of products containing chlorophacinone and used
as rodenticides in cereal grains in 2007 and bromadiolone in
Cereal grains and in form of commercial bait in 2013; these practices caused severe
damages over birds populations, hare, canines; amongst others[6-7]. Currently it is
Obvious that intentional application of poisoned baits keep posing a threat to fauna and
may entail far-reaching consequences over populations nationally and around the world.
[30,4,16].
Conclusions.
Poisoned baits against fauna remains a prevalent practice.
Legal prosecution of these malpractices may promote the conflict between economic
and environmental interests.
It is necessary to seek a solution based upon the exchange of information and
surveillance.