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A laboratory report submitted in partial fulfillment of the requirement in ZOO 11
Fundamentals of Zoology under Prof. Maria Claret Lauan-Tsuchiya2nd Semester 2015-2016
Abstract
The animal cell is composed of multiple parts and each one performs a different and vital
function.
This scientific paper targets to explain the observation made by the researchers and explain the
mechanism that regulates the flow of materials in and out of the cell through the cell membrane
and differentiate diffusion from osmosis.
Introduction
Cell is the basic unit of life. There are many different types of cells and its functions varies in its
form, it can be classified according to its shape and arrangements and further categorize in to two
types: Prokaryotic and Eukaryotic. Prokaryotic cells like bacteria and cyanobacteria have no
organized nucleus because they lack nuclear membrane and usually do not have membrane
bound organelles. Eukaryotic cells like plant cell and animal cells are more complex and exhibit
a well- defined nucleus surrounded by a numerous membrane bound organelles. In higher form
of multicellular animals, eukaryotic cells may be further categorized into two types: germ cells
or reproductive cells are cells with haploid set of chromosomes and somatic cell or general body
are cells with diploid set of chromosomes.
Cells may contain several other types of organelles, which includes cell wall, cell membrane,
nucleus nuclear membrane, cytoplasm, mitochondria, the endoplasmic reticulum, the Golgi
apparatus, ribosomes, and lysosomes, vacuole and some might even have a chloroplasts Each of
these organelles performs a specific function critical to the cell's survival. Most cells size range
between 1 and 100 micrometers and can only be seen through the help a microscope. (Cell
structure, n.d)
Part 2. Diffusion and Osmosis. Section 1 Diffusion. To see the process of diffusion a petri dish
was filled with water, a small portion of ink was then dropped and the movement of the ink was
observed.
Section 2 Osmosis. In the observation of osmosis a small portion of a shell, just enough to fit the
end of glass tubing is removed from an egg by chipping a circular opening on the rounded end of
the egg to expose the membrane lining. At the bottom part of the egg another larger portion of
the shell is removed, making sure not to pierce the membrane lining. The lower part of the egg
where the bigger portion where the egg shell is removed is mounted on a clay triangle inside a
1000 ml beaker and the glass tubing was inserted just about an inch inside the egg and a melted
wax was poured to prevent escape of the egg content. A 600 ml of water is then poured to the
beaker semi submerging the egg. After 8 hours, the changes in the rise of egg white was observed
and counted with the help of a ruler.
Section 3 Hemolysis and Crenation of human re blood cells. To be able to observe the process
of hemolysis and crenation of human red blood cell two drops of distilled water, 0.9% sodium
chloride and 10% of sodium chloride was placed onto individual slides and each slide was added
with one drop of human blood. Each slide is then observed under the microscope.
Section 2. Structure of the plasma membrane. The plasma membrane is the boundary between
the cell and its environment. It regulates what enters and exits the cell.
n.d.) every time the experiment is repeated the membrane that formed on the surface of the milk
seems to be growing thicker and darker.
Once the membrane lining of the egg was exposed and submerged under distilled water the water
slowly diffuses into the cell because of greater osmotic pressure outside the membrane. After 8
hours the researchers once again observed the change in the height of the displaced egg albumin
with the help of the ruler.
Fig. 4. Osmosis
Section 3. Hemolysis and crenation of human red blood cells. The human plasma has a solute
concentration of 0.9% sodium chloride. If a blood is placed in a hypertonic solution water enters
the cell and causes it to burst and if blood is placed in a hypertonic solution water leaves the cell
and causes it to shrink. In the experiment where the blood cell is put in a distilled water, water
(hypotonic) enters the cells causing it to burst, In the experiment with 10% sodium chloride the
water in the blood cell moved out of the cell causing the cell to shrink, and In the experiment
with
0.9% sodium chloride the water move in and out the cell with ease.
References:
10
CK-12. (n.d.). Prokaryotic and Eukaryotic cell. Retrieved from. http://www.ck12.org/biology/Prokaryoticand-Eukaryotic-Cells/lesson/Prokaryotic-and-Eukaryotic-Cells/
CellStructure.(n.d.) retrieved from. http://www.biologyjunction.com/cell_functions.htm
Instant answer. Retrieved from: http://www.instantanswer.org/why-dont-oil-and-water-mix_479.html
What is diffusion? Retrieved from: http://www.austincc.edu/biocr/1406/labm/ex5/prelab_5_1.htm
MilkFacts. Retrieved from: http://www.milkfacts.info/Milk%20Composition/Fat.htm