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Product Information PrimeQ™ KSOM Mouse Embryo Culture Medium (1X) w/ AA and Phenol Mouse embryo culture

Product Information PrimeQ™ KSOM Mouse Embryo Culture Medium (1X) w/ AA and Phenol

Mouse embryo culture medium. Sterile-filtered.

Catalog No.

 

GSM-5140

Unit Size

 

50 mL / 1X

Shipping

 

Frozen on dry ice

Storage

 

Store frozen at -20ºC to -80 ºC Do not use a frost-free freezer. The product should be used immediately after thawing, but can be stored at 2-8ºC for 1 week.

 

Shelf Life

 

1.5 Year at -20C to -80(frozen); 1 week at 4C (thawed)

Quality

     

Testing

Mouse embryo assay (MEA ) for assessing toxicity and ability of the media to support the development of one- and two-cell mouse embryos to expanded blastocysts;

 

Independently tested by Charles River Laboratories

 

Safety

 

BioSafety Level: 1

Information

Safety conditions pertaining to well characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment should be used. Lab coats and gloves should be worn at all times. Face protection is recommended when thawing a frozen vial stored in a liquid nitrogen freezer.

 

Intended Use KSOM mouse embryo medium is commonly used for in-vitro mouse embryo culturing KSOM and has been shown to support the development of mouse preimplantation embryos in culture. Additional studies have also shown that KSOM supplemented with amino acids increases the rate of blastocyst stage development. 1,2,3 For Research Use Only (RUO). Caution: Not intended for human or animal diagnostic or therapeutic uses.

Directions for Use Thaw frozen bottle of medium in a refrigerator (2C to 8C) overnight or slowly at room temperature. Gently swirl thawed medium to mix and warm to 37C prior to use. Perform all actions using aseptic culture conditions. Once thawed, the medium can be stored for 1 week at 2C to 8C. Do not use past expiration date. Medium used past the recommended expiration date may not perform optimally.

Note: The pH of sodium bicarbonate (NaHCO3) only buffered formulations will rise upon exposure to air and should be adjusted as needed prior to use.

Equivalent to Millipore Cat. No. MR-021-D

References:

1 JA Lawitts and JD Biggers, (1993) Culture of preimplantation embryos. Methods Enzymol., 225, 153-164. 2 GT Erbach, et al., (1994) Differential growth of the mouse preimplantation embryo in chemically defined media. Biol. Reprod., 50, 10271033.

3 Y Ho, et al., (1995) Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Mol. Reprod. Dev., 41, 232238